The Effects of Gamma
Radiation on Hop
By Brooke GettyMasters Student-OSU Aroma Hops Breeding
Program
Overview of Hops➢ Hop (Humulus lupulus L.)➢ Dioecious, clonally
propagated➢ Lupulin- bittering and
flavoring agent in beer
The Problem
➢ Complex chemical profile➢ Development time ~10-14 yrs➢ Limited germplasm➢ Specialized equipment➢ Long juvenility period
Mutation Breeding
Mutation Breeding➢ Induced Mutagenesis: The use of radiation
or chemical mutagens to develop variants with desirable traits
➢ Physical - radiation➢ Chemical - EMS- Ethyl methanesulfonate➢ Used since the 1930’s, ➢ Currently > 3,000 mutant cultivated varieties
(http://mvgs.iaea.org)
Mutation Breeding➢ Previous literature suggested ~5.0 Gy to
induce mutation.○ Al-Safadi,(2000)- Potatoes: 2.5 Gy (microtuber
formation)○ Y.Lokko, H.Amoatey(1998)- Pineapple: 45 Gy (Salt
tolerance)○ Charbaji et al., (1999)- Grape vine: 2,5,7 Gy
(Growth)
Goals
➢ Develop replacement hop cultivars
➢ Find markers associated with important traits in collaboration with Dr. John Henning
Objectives
➢ Determine effect of 3 gamma radiation doses(2,3,4 Gy) on Cascade and Centennial hops.○ Chemical and sensory profile○ Survival○ Morphological traits○ Disease and insect resistance
➢ Obtain markers for important traits
Materials and MethodsMicropropagation and irradiation of hops
Materials and MethodsGrowth, observation & transfer into sterile potting mix
Field Nursery
Previous Observations
Irradiation ResultsCascade
Field Observations
Field Observations
Overview
➢ Difficult to breed replacement cultivars due to complex chemical profile
➢ Working towards developing mutation breeding as a potential tool
➢ Obtain markers for important traits
THANK YOU!➢ Dr.Townsend➢ Dr.Henning➢ Indie Hops➢ Dr. Reed➢ Dr.Shellhammer➢ Dr.Zemetra➢ German monks for
putting hops in beer!!!!!
Literature CitedSax,K.1963.The stimulation of plant growth by ionizing radiation. Radiation Botany, 3(3): 179-186.
http://mvgs.iaea.org