Cell Reports, Volume 26
Supplemental Information
PDGF-B Is Required for Development
of the Glymphatic System
Anne Sofie Munk, Wei Wang, Nicholas Burdon Bèchet, Ahmed M. Eltanahy, Anne XiaoanCheng, Björn Sigurdsson, Abdellatif Benraiss, Maarja A. Mäe, Benjamin TravisKress, Douglas H. Kelley, Christer Betsholtz, Kjeld Møllgård, Anja Meissner, MaikenNedergaard, and Iben Lundgaard
FigureS1.VentralviewoftracerinfluxinP1,P7andP14mice,RelatedtoFigure1
DorsalviewofwholebrainsshowingdistributionofCSFtracersFITC-dextran(dextran-3kDa)
and ovalbumin-Alexa647 (OA-45 kDa) 60 min after injection into the cisterna magna. P1
mousebrain(toppanel)showsdistributionoftracersaroundtheCircleofWillisandalongthe
proximalpartofthemiddlecerebralartery(MCA). Imagesofdextran-3kDaandOA-45kDa
tracersinP7(middlepanel)andP14brain(bottompanel)showtracerdistributionaroundthe
CircleofWillisandtheMCAs.
FigureS2.WesternblotsofAQP4vessel–andparenchymalfractionsduringdevelopment,
RelatedtoFigure3
A-D) RepresentativeWestern blots of parenchyma fractions and isolated vessel fractions
incubatedwithprimaryantibodiesagainstaquaporin4(AQP4,38kDa);andb-tubulin(52kDa).
A)Theblotwascoveredstartingfromthedottedlineandupwardstoavoidstrongsignalfrom
off-targetbands.CTX,cortex;HPC,hippocampus;P,postnatalday;M,proteinmarker;CTR
Ag.,AQP4controlantigen.
FigureS3.WesternblotsofAQP4vessel–andparenchymalfractionsfromPdgfbret/ret
mice,RelatedtoFigure6
A-B) Representative Western blots of parenchyma fractions and isolated vessel fractions
incubatedwithprimaryantibodiesagainstaquaporin4(AQP4,38kDa);andb-tubulin(52kDa).
A)Theblotwascoveredstartingfromthedottedlineandupwardstoavoidstrongsignalfrom
off-targetbands.CTX,cortex;HPC,hippocampus;M,proteinmarker;CTRAg.,AQP4control
antigen;WT,wildtype;R/R,Pdgfbret/ret;R/+,Pdgfbret/wt.
