PHAGE STATUS REPORT #2
Anna Phipps and Janna Anderson
PHAGE NAME AND REASONING
We decided name our phage Banana7 because Banana rhymes with both our names.
SOURCE OF OUR PHAGE
We got our phage from Dr. K’s clean compost sample
PLAQUE MORPHOLOGY
i. Size: 1.5 mm
Ii. Appearance: Our phage is clear without a halo
TITER OF HTL
The titer of our HTL is 1.95*10 ^7
DNA ISOLATION
A. Concentration: 1.95µg/µl
B. Yield: 19.5µg
C. : 0.01733
D.: Restriction Digest Results: PstI:2cm, SalI:2cm, EcoRv: 2cm, NcoI:3cm,
2ND DNA GEL RESULTS
Our second round of using restricting enzymes on our gel was very faint, but the results were interesting.
Controle: 1cm, BanHl:0.5cm, Clal: 1cm, EcoRl:1cm, Haell &Hindll: 1cm.
DOES YOUR P HAGE LOOK L IKE ANY OTHER PHAGES FROM THE CLASS OR
DATABASE?
As far as we have looked, our phage does not look like any others from the class or the database.
A N Y T H I N G E L S E T H AT I S I N T E R E S T I N G A B O U T Y O U R P H A G E ?
Our phage is a siphoviridae. It looks like the head has been broken into and it is now
dead.
WHY SHOULD THE CLASS SELECT YOUR PHAGE AS THE ONE TO
SEQUENCE?
I would say that our phage should be picked because of its clarity and awesome name.