Protein-RNA complexes, ALS and aging
Jernej Ule, ulelab.info
iCLIP: nucleotide-resolution crosslinking and immunoprecipitation
Konig et al., NSMB, 2010Haberman et al, Gen biol, 2017
Tollervey et al., Nat Neuro, 2011data at http://icount.biolab.si
TDP-43 regulates splicing via UG repeat sequences
regulated exon
Splicing of TDP-43-regulated alternative exons in human brain
Tollervey et al., Genome Res, 2013
disease-specific
aging-associated
Alternative splicing affected by aging and/or disease in human brain
Tollervey et al., Genome Res, 2013
disease-specific
aging-associated
Analysis of cell-type specific gene expression
Tollervey et al., Genome Res, 2013
Soreq et al., Cell Rep, 2017
Analysis of cell-type specific gene expression upon aging
Soreq et al., Cell Rep, 2017
Shifts in regional expression profiles of glia-specific genes upon aging
Summary
Analysis of splicing changes revealed a common component of aging and neurodegeneration
Dramatic decrease of neuron-specific genes is a hallmark of neurodegeneration (as expected)
Distinct changes in cell type-specific expression profiles are a hallmark of aging
Microglia-specific genes increase, and neuron-specific genes slightly decrease their expression
Astrocyte and oligodendrocyte-specific genes shift their regional expression patterns
Cell-type specific gene patterns in aging
Mina Ryten, John Hardy,UK Brain Expression Consortium
James Rose, Colin Smith,
University of Edinburg
LilachSoreq
RickiePatani
Chris ShawKCL, London
James Tollervey
Aging & TDP-43 in ALS