Puccinia triticina Puccinia triticina virulencevirulence
phenotypes phenotypes present in Uruguay present in Uruguay
during 2004-2010during 2004-2010
Silvia GermánSilvia Germán
La EstanzuelaLa Estanzuela
2011 North American Rust Workers Meeting2011 North American Rust Workers MeetingSt. Paul, Minnesta, USASt. Paul, Minnesta, USA
June 12, 2011 June 12, 2011
Wheat leaf rustWheat leaf rust((Puccinia triticinaPuccinia triticina))
SamplesSamples• Trap nurseries Trap nurseries • ExperimentsExperiments• CropsCrops• 1 – 2 single pustule isolates per sample 1 – 2 single pustule isolates per sample
Differential setDifferential setLr 1 2a 2c 3Lr 1 2a 2c 3Lr 9 16 24 26Lr 9 16 24 26Lr 3ka 11 17 30Lr 3ka 11 17 30Lr 10 20 .....Lr 10 20 .....
NomenclatureNomenclatureLong and Kolmer, 1989Long and Kolmer, 19893 letter3 letter Prt Prt code + code +Virulence to Virulence to Lr10 andLr10 and Lr20Lr20 MDTMDT
MCR-10MCR-10 MFP-20MFP-20
TDD-10,20TDD-10,20
METHODOLOGYMETHODOLOGY
Race 2004 2005 2006 2007 2008 2009 2010 Total
Total Ner of isolates 100 175 134 122 124 81 134 870
No of races 19 30 24 28 20 16 12 69
No of races freq > 5% 5 5 4 3 6 6 8 15
No races freq >10% 3 3 3 3 5 4 4 12
Number of Number of P. triticinaP. triticina isolates studied, tolal numer of races isolates studied, tolal numer of racesand races present with more than 5 and 10% frequencyand races present with more than 5 and 10% frequency
in Uruguay. 2004-2010.in Uruguay. 2004-2010.
Avirulence/virulence formulae of more frequent races, Avirulence/virulence formulae of more frequent races, and year of first detectionand year of first detection
First
detection
MCD-10 2a,2c,3ka,9,11,16,20,24,30 / 1,3,10,17,26 2001
MCD-10,20 2a,2c,3ka,9,11,16,24,30 / 1,3,10,17,20,26 1999
MCP-10 2a,2c,9,11,16,20,24 / 1,3,3ka,10,17,26,30 2000
MDP 2a,2c,9,10,11,16,20,26 / 1,3,3ka,17,24,30 2002
MFP 2a,2c,9,10,11,16,20 / 1,3,3ka,17,24,26,30 2001
MDP-10,20 2a,2c,9,11,16,26 / 1,3,3ka,10,17,20,24,30 2004
MFP-10,20 2a,2c,9,11,16 / 1,3,3ka,10,17,20,24,26,30 2004
MDP-20 2a,2c,9,10,11,16,26 / 1,3,3ka,17,20,24,30 2004
MFP-20 2a,2c,9,10,11,16 / 1,3,3ka,17,20,24,26,30 2005
MDR-10,20 2a,2c,9,16,17,26 / 1,3,3ka,10,11,20,24,30 2003
MDT 2a,2c,9,10,16,20,26 / 1,3,3ka,11,17,24,30 2010
MDT-10,20 2a,2c,9,16,26 / 1,3,3ka,10,11,17,20,24,30 2007
MFT-10,20 2a,2c,9,16 / 1,3,3ka,10,11,17,20,24,26,30 2007
TDT-10,20 9,16,26 / 1,2a,2c,3,3ka,10,11,17,20,24,30 2010TFT-10,20 9,16 / 1,2a,2c,3,3ka,10,11,17,20,24,26,30 2010
Race Avirulence / Virulence formula
Frequency of important Frequency of important Puccinia triticinaPuccinia triticina isolates isolatesand affected cultivares Uruguay. 2004-2010.and affected cultivares Uruguay. 2004-2010.
First
detection 2004 2005 2006 2007 2008 2009 2010 Epidemic on
MCD-10 2001 15.0 8.0
MCD-10,20 1999 6.0 2.3 0.7 3.3 I Mirlo
MCP-10 2000 28.0 15.4 1.5 1.2 K.Don Enrique
MDR-10,20 2003 17.0 32.0 20.1 3.3 1.6I.Churrinche I.Torcaza
MDP 2002 4.0 3.4 0.7 4.1 7.3 13.6 10.4
MFP 2001 6.0 0.6 1.6 4.0 21.0 30.6
MDP-10,20 2004 4.0 10.9 18.7 16.4 11.3 3.7 5.2
MFP-10,20 2004 1.0 2.2 1.6 16.9 18.5 6.7
MDP-20 2004 1.0 5.1 6.0 0.8 2.4 3.7 6.7
MFP-20 2005 1.7 28.4 4.1 12.9 13.6 1.5 I Tero
MDT-10,20 2007 23.8 17.7 8.6 2.2
MFT-10,20 2007 13.9 11.3 6.2 0.7
TDT-10,20 2010 14.9 Atlax
TFT-10,20 2010 11.9 Atlax
MDT 2010 7.5
Race
Frequency
Virulence formulae and year of first detection of other Virulence formulae and year of first detection of other Puccinia triticinaPuccinia triticina races present in Uruguay races present in Uruguay
during 2004-2010.during 2004-2010.
First First
Race Virulence formula detection Race Virulence formula detection
CCT 3,3ka,11,17,23,26,30 1997 MFD-10,20 1,3,10,17,20,24,26 2002
CGT 3,3ka,11,16,17,23,30 1991 MFR-10,20 1,3,3ka,10,11,20,24,26,30 2004
CHT 3,3ka,11,16,17,23,26,30 1997 MFT 1,3,3ka,11,17,24,26,30 2009
MBD-10,20 1,3,17,10,20 1998 MFT-20 1,3,3ka,11,17,20,24,26,30 2008
MBP-10,20 1,3,3ka,10,17,20,30 2006 MHP-10 1,3,3ka,10,16,17,26,30 2002
MCB-10 1,3,10,26 2004 MMD-10 1,3,9,10,17,26 2006
MCG-10 1,3,10,11,26 1989 MMD-10,20 1,3,9,10,17,20,26 2006
MCP-10,19 1,3,3ka,10,17,19,26,30 2006 MRD-10 1,3,9,10,16,17,26 2008
MCP-10,20 1,3,3ka,10,17,20,26,30 2002 SNG-10 1,2a,2c,9,11,24 2003
MCR-10 1,3,3ka,10,11,26,30 1989 SPG-10 1,2a,2c,9,11,20,24,26 2002
MCT-10 1,3,3ka,10,11,17,26,30 1992 TDD-10,20 1,2a,2c,3,10,17,20,24 1995
MDD-10,20 1,3,10,17,20,24 2002 TDG-10,20 1,2a,2c,3,10,11,20,24 1999
MDP-10 1,3,3ka,10,17,24,30 2006 TDP-10,20 1,2a,2c,3,3ka,10,17,20,24,30 2007
MDR-10 1,3,3ka,10,11,24,30 2005 TFP 1,2a,2c,3,3ka,17,24,26,30 2007
MDR-20 1,3,3ka,11,20,24,30 2002 TPR-20 1,2a,2c,3,3ka,9,11,20,24,26,30 2008
Year 1 2a 2c 3 9 16 24 26 3ka 11 17 30 10 20 19
2004 99.0 3.0 3.0 100.0 0.0 0.0 43.0 68.0 71.0 27.0 72.0 71.0 88.0 41.0 0.0
2005 98.9 3.4 3.4 100.0 1.7 0.0 65.1 39.4 81.1 46.3 57.7 81.1 86.3 65.1 0.0
2006 94.8 1.5 1.5 99.3 3.0 0.7 85.1 50.0 94.8 32.8 73.9 94.8 59.7 85.1 4.5
2007 100.0 12.3 12.3 93.4 9.8 0.0 88.5 42.6 86.9 55.7 83.6 86.9 82.0 82.0 1.6
2008 100.0 5.6 5.6 100.0 7.3 4.0 93.5 56.5 95.2 36.3 92.7 95.2 68.5 82.3 0.0
2009 97.5 3.7 3.7 100.0 3.7 3.7 93.8 67.9 98.8 21.0 97.5 98.8 42.0 59.3 0.0
2010 100.0 26.9 26.9 100.0 0.0 0.0 100.0 53.0 100.0 38.8 100.0 100.0 41.8 50.0 0.0
B 0-20% BI 20-40% I 40-60% IA 60-80% A 80-100%
% virulence% virulenceLr3bg Lr3bg high (2006 +)high (2006 +)Lr14a Lr14a 100100Lr14b Lr14b high highLr21 Lr21 00Lr23Lr23 int-highint-highLr25 Lr25 0 (2006 +, mixture)0 (2006 +, mixture)Lr39/41 Lr39/41 2009 need confirm2009 need confirmLr42 Lr42 2009 need confirm2009 need confirmLr47 Lr47 0 (2009 +)0 (2009 +)
APR genesAPR genesLr12Lr12 inefectiveinefectiveLr13Lr13 ineffectiveineffectiveLr22aLr22a effectiveeffectiveLr22b Lr22b ineffectiveineffectiveLr35 Lr35 effectiveeffectiveLr37Lr37 ineffectiveineffectiveLr34Lr34 50-60 M 50-60 MLr46Lr46 70-80 MSS 70-80 MSS
Virulence frequency to Virulence frequency to LrLr genes. 2004-2010. genes. 2004-2010.
Field effectiveField effectiveLr29 Lr29 00Lr32 Lr32 40 MRMS40 MRMSLr33 Lr33 60 MRMS60 MRMSLr36 Lr36 00Lr45 Lr45 00Lr47 Lr47 00Lr51 Lr51 00
Foto grupo de trabajoFoto grupo de trabajo
RichardRichardGarcíaGarcía
NoeliaNoeliaPerezPerez
FernandoFernandoPereiraPereira
Thank youThank youGraciasGracias