RESTRICTION ENZYME ANALYSIS
IMBB 2013
RE: recap
NEBcutter-online tool (free)
• This tool will take a DNA sequence and find the
largest, non-overlapping ORF.
• Can select restriction enzymes that cut the
sequence from a wide range of commercial RE.
• Can customize restriction digestion.
• Can visualize restriction fragments on a virtual
agarose gel.
• http://tools.neb.com/NEBcutter2/
RFs