Review on Nanoparticle Probes with Surface Enhanced
Raman Spectroscopic Tags (SERS Dots) for Cellular
Cancer Targeting
Dae Hong Jeong(Department of Chemistry Education, Seoul National University; jeongdhts'snu.ac.kr)
Abstract
This article is a review version of the article published on Oct. 2006 at
Analytical Chemistry, 78, 6967-6973. We have developed biocompatible,
photostable, and multiplexing-compatible surface-enhanced Raman
spectroscopic tagging material (SERS dots) composed of silver
nanopartic1e-embedded silica spheres and organic Raman labels for cellular
cancer targeting in living cells. SERS dots showed linear dependency of
Raman signatures on their different amounts, allowing their possibility for
the quantification of targets. In addition, the antibodyconjugated SERS dots
were successfully applied to the targeting of HER2 and CDIO on cellular
membranes and exhibited good specificity. SERS dots demonstrate the
potential for high-throughput screening of biomolecules using vibrational
information.
Key Words: SILVER NANOPARTICLES, SURFACE-ENHAl'\JCED
RAMAN SCATTERING, ANTIBOTY- ANTIGEN REACTION
Review on Nanoparticle Probes with Surface Enhanced
Raman Spectroscopic Tags (SERS Dots) for Cellular
Cancer Targeting
01 ~~ ~ 2006\i 10-%1. ~~:A1 Analytical Chemistrys] 78~ 6967-6973~O1l
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(b) MPTS7} ]i~01] ~lcj~ ~, (c) 2. g £~~ ~, (d) 2. 0] £~B ~.Q-] ~
rJ1B A}~.
-=:2 13 3. SERS dots.Q-] TEM A}~: (a) 4-MT7} ]i7:] B SERS dots,
(b) 2-NT7} ]iAl B SERS dots, (c) TP7} ]i7:l B SERS dots.
-=:213 4. ~cj51} ~g AJ}7] Zi.Q-] SERS dots~ SERS ~~E~:
(a)4-MT, 2-NT, TP Zf ]i~ SERS dots,
(b) TP2] *£01] u:j-~ A~E'iJ2] A-J]7] ~}0l.
-=:2 ttl 5. ~ cj 51} ~ g ~ ~~ SERS dots.Q-] SERS ~~ E ~:
(a) 4-MT, 2-NT, TP Zf ]i~ SERS dots,
(b) TP7} ]i7:] B SERS dot.Q-] OJoj] u:]-~ ~~ E ~.Q-] A-J] 7] ~}o]
-=:213 6. A-J].:£oJ]Al '5J~1l-'4 <i1~B SERS dot.Q..s. HER2 ~ CDIO ~A-J].:£ p}71
~ ~~~ ~JJl-:
(a) SERS dot4MT-HER2-'4 l:lR 0J~ MCF-7,
(b). SERS dotTP-CDlOJJl- l:lR 0J~ SP2/0,
(c) SERS dotTP-CDlOJ1} l:lRoJ~MCF-7,
(d) SERS dot4MT-HER2-'4 l:lRoJ~ SP2/0,
(e) SERS dot4MT-HER2-'4 l:lRoJ~ NHBE,
(f) SERS dotTP-CDlOJ1} l:IB 0J~ NHBE
Ag-embeddedsilica
OMe
Meo-rMe
HS
Ramanlabel
AgNO,NH40B
Ethyleneglycol
Rednction
\oO-ki~SH
?/
oHS~Si:_O
o\
MeOMeO-Si~SH
IOMe
Silica(180-210nm)
H,N5vol%APTS
NH,~,~-----
SERSDots
Sodiumsilicate
TEOS
l. Spacer
2MIPNS
3. Antibodies (Ber2,CDIO)
Targeting
Cell Membrane
:J..~ 2.
:J..~ 3.
400 600 800 1000 1200 1400 1600 1800
(b)
Raman Shift/ em-I
10nM
1nM
100 pM
400 600 800 1000 1200 1400
Raman Shift fernl
1600 1800
::L ~ 5.
(a)
4-MT T 10000counts
~
==~ 2-NT T8000 counts.....
;<;::'"=...-=-
T9000countsTP
400 600 800 1000 1200 1400 1600 1800
Raman Shift / em·t
.:-:/
o 5 10
SERSDot Concentration (mg/ml)
180
.~~ 900
..:
NNo
(J)(J)(J)
(b)
1000 1100-I
Raman Shift / em
.=:L~ 6
(a)
§.40
40
Length {f.tm
AS
FS
600 800 1000 1200 1400 1600 1800
Raman Shift I cm'
(b)
§.40
40
Length !1Ull
lsl/h/)l11600 900 1200 1500
Raman Shift I cm'
(c)
40
Length I urn
600 SOD 1200 \400 1600 1800
Raman Shift I ern"
(d)
(e)
3J 3S 40 45 Sl 55
I
\L.........-..--.. _
600
600
800 1000 1200 1400
Raman Shift I ern"
900 1200 1500
Raman Shift I cm'1800
203
m~ 20
Length I urn
ABC 0
,-~~~-----
---,-----.--,-~......---'r-·,..·~--·~--T-----..--·
1200 1~C'0 I·;C'~' 1:00
Raman Shift I cm '