Application Cost Platform/Protocol Note
3’ mRNA-SeqCell capture/RT/library prep $1,990/Sample 10x Genomics
Chromium 500-10,000 cells/sample
5’ V(D)J + mRNACell capture/RT/library prep
(B and T cell enrichment)$2,460/Sample 10x Genomics
Chromium 500-10,000 cells/sample
Sequencing for 10x Genomics3’ single-cell library $1,990 for 350M reads* Illumina NovaSeq Custom 91bp sequencing
Sequencing for 10x Genomics3’ single-cell library $3,780 for 400M reads* Illumina NextSeq Custom 98bp sequencing
Sequencing for 10x Genomics5’ V(D)J + 5’ mRNA single-cell library $3,060 for 350M reads* Illumina NovaSeq 2x150bp sequencing
Full transcript single cell mRNA-SeqCell lysis/RT/QC
Library prep and QCSequencing (2M reads/cell)Sequencing (1M reads/cell)
$23/cell$10/cell$10/cell
$5.25/cell
SMART-Seq A minimum of2x96-well plates
* Price includes bioinformatics QC, alignment, quantification and visualization. For libraries generated by Genomics Core.
Single Cell Genomics
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Application Cost* Sample Minimum Sample Requirements Platform Read
TypeAverage Output
mRNA-Seq$349/Sample
$298/sample
6
> 40
RIN > 8500 ng – 1 µg NextSeq 1x75bp 25M reads
Ultra low-input mRNA-Seq $375/Sample 6 RIN > 8
50 ng – 400 ng NextSeq 1x75bp 25M reads
Total RNA-Seq $599/Sample 6 No mimimum RIN750 – 2 µg NextSeq 1x75bp 50M reads
RNA-Seq for FFPE $349/Sample 6DV200 > 30%
Total RNA: 50 – 400 ngFFPE: 250 – 500 ng
NextSeq 1x75bp 10M reads
Small RNA-Seq(UMI quantification) $375/Sample 6 RIN > 8 preferable
300 ng – 1.5 µg NextSeq 1x75bp 10M reads
*Price includes sample QC, library preparation, library QC, sequencing and basic bioinformatics quantification.
Next-Gen Sequencing-RNA
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Application Cost* Sample Minimum
SampleRequirements Platform Read
TypeAverage Output
Human Whole Genome
Sequencing (FFPE compatible)
$1,200/sample 61 -2 µg non-degraded gDNA
1 - 3 µg FFPE DNA; fragments > 1500 bp
NovaSeq 2x150bp 30X coverage
Human Whole Exome Sequencing(FFPE compatible)
$499/Sample
$449/sample
8
> 40
0.5 – 1 µg non-degradedgDNA
1 - 2 µg FFPE DNA; fragments > 1500 bp
NovaSeq 2x150bp 120X coverage
ATAC-Seq$489/sample
$829/sample8
5,000 – 50,000 cells in culture media; > 90%
viability
NextSeq
NovaSeq
2x75bp
2x50bp
2x40M reads
2x120M reads
16S Microbiome Amplicon-Seq $60/Sample** 50
10 µl/sample in an Eppendorf twin.tec plate
in columnsMiSeq 2x300bp 10K reads
ITS Mycobiome Amplicon-Seq $60/Sample** 50
10 µl/sample in an Eppendorf twin.tec plate
in columnsMiSeq 2x300bp 10K reads
*Price includes sample QC, library preparation, library QC, sequencing and basic bioinformatics quantification.** Price includes library preparation, pooled library QC and sequencing.
Next-Gen Sequencing-DNA
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Sequencing Platform Read Output Read type Cost*
NextSeq 400M 1x75bp $1,870/Sequencing run
NextSeq 2x130M 2x75bp $1,280/Sequencing run
NextSeq 2x400M 2x75bp $3,540/Sequencing run
NovaSeq 2x1300M 2x50bp $7,290/Sequencing run
NovaSeq 2x1300M 2x150bp $11,490/Sequencing run
NovaSeq 2x3300M 2x50bp $13,590/Sequencing run
NovaSeq 2x3300M 2x150bp $21,290/Sequencing run
MiSeq 2x15M 2x25bp $1,080/Sequencing run
MiSeq 2x25M 2x300bp $2,180/Sequencing run
*Only pooled libraries accepted. Full runs only (no shared runs). Price includes fragment analysis, fluorometric quantification, and sequencing of pooled library. One-time demultiplexing/FASTQ generation is included.
Sequencing Only
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Application Reaction Cost Platform
Gene expression 48 (targets*) x 48 (samples) $500/Plate Fluidigm BioMark HD
Gene expression 96 (targets*) x 96 (samples) $1,400/Plate Fluidigm BioMark HD
Genotyping 48 (targets*) x 48 (samples) $550/Plate Fluidigm BioMark HD
Genotyping 96 (targets*) x 96 (samples) $1,100/Plate Fluidigm BioMark HD
Human/rodent miRNA gene expression panel 745 (genes) x 3 (samples) $980/Plate QuantStudio 12K
*Primer pairs must be provided.
High-Throughout qPCR
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DNA Type Assay Concentration Range Size Range Cost Platform
dsDNA High Sensitivity 50 pg/µl – 5 ng/µl 25 – 5000 bp$20 for row + $5/sample
up to 12 samplesFragment Analyzer
dsDNA High Sensitivity 100 pg/µl – 10 ng/µl 50 – 7000 bp$60 for chip +
$5/sample up to 11 samples
Agilent Bioanalyzer
dsDNA Standard Sensitivity 5 ng/µl – 100 ng/µl25 – 5000 bp $20 for row + $5/sample
up to 12 samplesFragment Analyzer
dsDNA DNA 1000 0.5 ng/µl – 50 ng/µl 25– 1000 bp$20 for chip +
$5/sample up to 12 samples
Agilent Bioanalyzer
gDNA High Sensitivity 50 pg/µl – 5 ng/ml 50 – 40,000 bp$20 for row + $8/sample
up to 12 samplesFragment Analyzer
gDNA Standard Sensitivity 25 ng/µl – 250 ng/µl 75 – 48,500 bp$20 for row + $8/sample
up to 12 samplesFragment Analyzer
DNA Fragment Analysis &RNA Integrity
RNA Type Assay Concentration Range Cost Platform
Total RNA High Sensitivity 50 pg/µl – 5 ng/µl$20 for row + $5/sample up to
12 samplesFragment Analyzer
Total RNA RNA Pico 50 pg/µl – 5 ng/µl$60 for chip + $5/sample up to
11 samplesAgilent Bioanalyzer
Total RNA RNA Nano 5 – 500 ng/µl$20 for chip + $5/sample up to
12 samplesAgilent Bioanalyzer6
Assay Range Cost Platform
dsDNA High Sensitivity10 pg/µl – 100 ng/µl;
1-20 µl$6/sample Qubit
dsDNA Broad Range100 pg/µl – 1 µg/µl;
1-20 µl$6/sample Qubit
ssDNA*50 pg/µl – 200 ng/µl;
1-20 µl$6/sample Qubit
RNA High Sensitivity250 pg/µl – 100 ng/µl;
1-20 µl$6/sample Qubit
Fluorometric Quantification
*Also detects dsDNA and RNA. For accurate ssDNA concentration, the dsDNA and RNA assay kits must be used to detect dsDNA and RNA contamination.
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Application Type* Cost
Human cell line authentication** Genomic DNA $70/sample
Human cell line authentication** Dried cell pellet $90/sample
Human cell line authentication Analysis $25/sample
Mycoplasma contamination detection Genomic DNA $70/sample
Mycoplasma contamination detection Dried cell pellet $90/sample
* Inquire about fresh or frozen cells, or if you wish to do both Cell Line Authentication and Mycoplasma Contamination Detection on the same sample.** Human cell line authentication includes a mouse marker for cross-contamination detection.
Cell Line Authentication &Mycoplasma Contamination Detection
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Service Cost
RNA-Seq bioinformatics package$1,000 for first pairwise comparison for internal sequencing ($1,350 for external sequencing); $600 each for additional
comparisons
Custom bioinformatics service $125/hour; 8 hour minimum
Extra demultiplexing/FASTQ generation $60
Custom laboratory service $60/hour
Bioinformatics Analysis &Self-Use Equipment
Instrument Cost
ABI QuantStudio 12K Real-Time PCR System $20/hour
Eppendorf Vacuum Centrifuge $5/hour
Covaris M220 Focused Ultrasonicator $4/hour
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