siRNA

-24 hrs

Thymidine Release siRNA Thymidine

0

Release

-39 hrs -20 hrs -15 hrs-44 hrs 9 hrs

Mitosis

± Reversine 0.5 µMor AZ3146 2 µM

15 hrs

Wash-out15 hrs5 hrs 4 hrs 5 hrs 15 hrs

Bub1RNAi

Mad2RNAi

Rev0.5µM

AZ2µMCtrl

100 KDa

37

20

15

WB

33 hrs0

Drug Treatment

Bub1RNAi

Mad2RNAi

Rev0.5µM

AZ2µMCtrl

WB

57 hrs0

Drug Treatment

LC3-ILC3-II

Mad2

Actin

Bub1

B

A

100 KDa

37

siRNA Ctrl Bub1 Mad2

20

15 LC3-ILC3-II

Mad2

Actin

Bub1Ctrl Bub1 Mad2+ ZM447439

C

DIMR-90

15 KDa

37 Actin

Ctrl RevLC3-ILC3-II

Lymphoblastoid

15 KDa

100 Vinculin

Ctrl RevLC3-ILC3-II

LC3-ILC3-II

Actin

Ctrl Rev AZBJ-hTERT

15 KDa

37

E

F

Santaguida_Supplemental Figure 3