SUPPLEMENTARY FIGURE LEGENDS
Supplementary Figure 1. (a) NECs and CECs isolated from all five patients were incubated on Matrigel for 16 h after irradiation with 4 Gy, and
then photographed. (b) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, RT-PCR was used to
assess the mRNA expression levels of MMP2, plasminogen and the 18S rRNA. The experiments were repeated in triplicate and the data shown
represent typical results. (c) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, zymography was
used to analyze the activities of MMP-2 and MMP-9. The experiments were repeated in triplicate, and the data shown represent typical results. (d)
NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was used to analyze the generation of
angiostatin. The results from three independent experiments are expressed as means ± SEM.
Supplementary Figure 2. (a) NECs and CECs isolated from all five patients were irradiated with 4 Gy. After incubation for 24 h, ELISA was
used to analyze the generation of IL-6. The results from three independent experiments are expressed as means ± SEM.
Supplementary Figure 3. NECs and CECs isolated from patient A were incubated on Matrigel for 16 h after irradiation with various doses of IR
(2, 3, 4, 6, 8 or 10 Gy), and then photographed.
NECs
CECs
Cont 4 Gy
Patient A
Cont 4 Gy
Patient B
Cont 4 Gy
Patient C
Patient D Patient E
Cont 4 Gy Cont 4 Gy
Supplementary figure 1.
NECs
CECs
(A)
4 Gy
A B C D E
NECs
18S rRNA
MMP2
Patient
- + - + - + - + - +
18S rRNA
Plasminogen
CECs
A B C D EPatient
4 Gy - + - + - + - + - +
Supplementary figure 1.
Zymography
MMP2
4 Gy - + - + - + - + - +
NECs
A B C D EPatient
MMP9
(B)
(C) (D)
A B C D EPatient A B C D E
NECs CECs
Generation of angiostatin
Supplementary figure 2.
Generation of IL-6
A B C D EPatient A B C D E
NECs CECs
Supplementary figure 3.
NECs
Control 2 Gy 3 Gy 4 Gy 6 Gy 10 Gy
CECs
8 Gy