H3C O
O
NCH3
CH3
CH3
acetylcholine
Target agent: VX
S
POO
O
NO2
parathion
S
POS
O
CO2Et
CO2Et
malathion
VX
O
PH3C SO
N
CH3
CH3
CH3
CH3
O
PH3CFO
sarin
CH3
CH3
O
PH3CCN
N
tabun
CH3
CH3
O
PH3CF
O
somanCH3
CH3
CH3
CH3
Other chemical agents:
Pesticides:
H3C O
O
NCH3
CH3
CH3
acetylcholine
Target agent: VX
VX
O
PH3C SO
N
CH3
CH3
CH3
CH3
Acetylcholinesterase
S
POO
O
NO2
parathion
S
POS
O
CO2Et
CO2Et
malathion
O
PH3CFO
sarin
CH3
CH3
O
PH3CCN
N
tabun
CH3
CH3
O
PH3CF
O
somanCH3
CH3
CH3
CH3
Other chemical agents:
Pesticides:
Organophosphorus Compounds
Peptides that recognize VX
NH2-Ser-GG-Arg-GG-His-GG-His-PheNH2-Arg-GG-Ser-GG-His-GG-Ser-PheNH2-Trp-GG-His-GG-His-GG-Trp-PheNH2-Arg-GG-Tyr-GG-Arg-GG-Ser-PheNH2-Tyr-GG-Trp-GG-Tyr-GG-Trp-PheNH2-His-GG-His-GG-His-GG-Arg-Phe
HO
O
N
NH
NH2
HO
O
NH2
OH HO
O
NH2
NH
NH
NH2 HO
O
NH2 OH
HO
O
NH
NH2
HisSer Arg Tyr Trp
Dr. Cyndi Wells, Dr. Ben Warner, Los Alamos Natl. Lab.
Selectivity and Recognition: Peptide/metal receptors
S
PHO OHOH
O
PH3C OHOH
O
PHO OHOH
phosphates from soil methylphosphonic acidfrom nerve agents
thiophosphatesfrom pesticides
differentiate by peptide differentiate by metal
Peptide: hydrophobic/hydrophilic, shape, sizeMetal: functional group discrimination
O
PH3C S
O
N
O
PH3CHS
HON
OHOH
H2O +++
VXmethylphosphonic
acidmercaptoamine ethanol
Degradation Products:
