The Alfin-like PHD Zinc Finger Transcription Factor
Family in Arabidopsis Thaliana
Melissa Borkenhagen8 May 2006
HC70ALDR. Bob Goldberg
Gene AT5G20510
5’ UTR
EXONINTRON
T-DNA
LBb1FW
Chromosome 5
T-DNA INSERT ACCORDING TO SALK
3’ UTR
Gene AT3G42790
3’ UTREXONINTRON
T-DNA
LBb1
FW
Chromosome 3
T-DNA INSERT ACCORDING TO SALK
5’ UTR
In which plant organs are the genes active?
Gene AT5G20510
Gene AT3G42790
Consistent with GeneChip Data, both genes appear to be active in these plant organs.
Gene AT5G20510 Plant Genotypes
Strange double bandsWhat are the genotypes?
Only one T-DNA Insert
Plant 1 is the only Homozygous Mutant Plant
Separation of primers
Gene AT3G42790 Plant Genotypes
Plants 1 and 4 are Homozygous Mutants
Plant 5: Heterozygous?
Are there any observable phenotypic differences between
wild type and mutant plants?
No significant differences observed between wild type and mutant plants.
Wild Type
Mutant
Is continued work on this family of transcription factors
important?Research has shown that members of the
alfin-like PHD zinc finger transcription factor family may be influential in plant
development. Some appear to be particularly influential during seed
development, with a possible role in the differential seedling development in darkness and light. Thus, continued work on these proteins is important!