Innovative LC Column Technology for
Formats, Selectivities and Tools for MaximizedTechnology for
Resolution and SpeedTools for Maximized Productivity
Paul BoguszewskiEMEAI Product Specialist
Novi Sad, Serbia
Outline
• Introduction
• Background to Zorbax particle technologyBackground to Zorbax particle technology
• Chemistry/Selectivity Overview
• Format overviewFormat overview
• Sub 2 micron technology (RRHD and RRHT)
– Speed vs Resolutionp
• Superficially Porous Technology (PoroShell)
– Invigorating older LC systems
41 Years ago….• Hewlett Packard acquires the assets of Hupe & Busch/Germany.• The HP1010 is the first ever HPLC Instrument available
• The HP1010 Liquid Chromatograph weighs more than half a ton.• The columns are packed with the latest 57 μm packing material.
Zorbax Base
Confused?
Too Many Columns!
ZORBAX Eclipse Plus Columns: First Choicef f•Eclipse Plus Excellent first choice for
method development
• Long life from pH 2-9g p
• Superior peak shape with basic compounds
• High resolution and efficiency with 1.8, 3.5 and 5 μm columns
• Rigorous QA/QC testing for greater long• Rigorous QA/QC testing for greater long-term reproducibility
Eliminate Tailing and Maximize Resolution with Eclipse Plus ColumnsEclipse Plus Columns
Eclipse Plus C18, 4.6 x 50mm1
Mobile Phase: 65% ACN: 35% 25 mM phosphate buffer (pH 7.4)
Tf = 1.20
1. Nortriptyline2 Di lth l t
Tf = 4.86 Competitive C18, 4.6 x 50mm
2. Dipropylthalate
1
min0 0.5 1 1.5 2
12
2
min0 0.5 1 1.5 2
Superior peak shape and better selectivity with Eclipse Plus means more resolution, easier quantitation and better results in your separations.q y p
ZORBAX StableBond Bonding: Low pH
•Superior low pH stability – down to pH 1 (C18)
•Non-endcapped for selectivity and lifetime
•Patented sterically protecting bonding
•6 different selectivities
•Ideal for most sample types at low pH
Stable Bond C18, 5um (pH 0.8, 90oC)
ZORBAX Extend-C18 : High pH
• High efficiency and long life at high pH – up to pH 11.5
• Unique bidentate bonding and double endcapping Unique bidentate bonding and double endcappingprovides high pH stability
• More efficiency and better peak shape than l b d lpolymer-based columns
•Excellent peak shape with double endcapping
•LC/MS at high pH (ammonium hydroxide) with high efficiency
Silica supportSilica support
Zorbax Extend C18: Carbonate Buffer pH 10.
ZORBAX Bonus-RP : Something Different!
• Excellent peak shape for challenging basic compounds at low and mid pH
• Unique reversed-phase selectivity
• Novel bonding technology with embedded polar group and steric protectionpolar group and steric protection
• Usable in 100% aqueous mobile phases
•Triple endcapped•Triple endcapped
Agilent Has Three Types of Columns for UHPLC
RRHT columns: 1.8um columns for fast LC up to 600 bar • Designed for compatibility with the Agilent RRLC • Compatible with some UHPLC’s with pressure limits up to 800 bar
RRHD columns: 1 8um columns for up to 1200 barRRHD columns: 1.8um columns for up to 1200 bar• Designed for compatibility with the new Agilent 1290 Infinity LC• Useable up to 1200 bar• Fully UHPLC compatible
Poroshell 120 columnsPoroshell 120 columns• 600 bar pressure limit for use with HPLC/UHPLC• UHPLC type performance but excellent fit for 400 bar LC’s
Sub 2um ColumnsSub 2um Columns
Van Deemter Equation
C term: Resistance to mass transfer is improved by Reducing particle size – this is the current standard approach Improving diffusion
hei
ght
H
ll ti l
The smaller the theoretical plate height H, the better the Resolution!
retic
al P
late
Resulting van-Deemter curveH = A + B/u + C x u
large particle small particle
Theo
r
C) Resistance to Mass Transfer
H min
B)Longitudinal DiffusionA) Eddy Diffusion
linear flow uu opt
Van Deemter Curve: HETP vs. Volumetric Flow Rate
0.0030Column: ZORBAX Eclipse XDB-C18 Dimensions: 4.6 x 50/30mmEluent: 85:15 ACN:WaterFlow Rates: 0.05 – 5.0 mL/min
H = A + B/u + Cu
0.0020
0.0025 Temp: 20°CSample: 1.0μL Octanophenone in Eluent
)
5um
0.0010
0.0015
HET
P (c
m
3.5um
0.0000
0.0005 1.8um
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0Volumetric Flow Rate (mL/min)
Smaller particle sizes yield flatter curvesp yminimal shift to higher flow rates
Column Length & Particle Size
ColumnLength ( )
ColumnEfficiency N(5 )
ColumnEfficiency N(3 5 )
ColumnEfficiency N(1 8 )
Analysis Time*
(mm) N(5 µm) N(3.5 µm) N(1.8 µm)150 12,500 21,000 35,000
100 8 500 14 000 23 250Efficiency Analysis
-
33%100 8,500 14,000 23,250
75 6000 10,500 17,500
50 4 200 7 000 12 000
(N)
Pressure
Time
PeakVolume
-33%
-50%
67%50 4,200 7,000 12,000
30 N.A. 4,200 6,500
Volume -67%
-80%
90%
SolventUsage
15 N.A. 2,100 2,500 -90%
* Reduction in analysis time compared to 150 mm column; all columns 4.6-mm i.d.educt o a a ys s t e co pa ed to 50 co u ; a co u s 6 d
RR High ThroughputRR High Throughput
Rapid Resolution High Throughput
Parameter RRHT 1.8um, 600bar
RRHD1.8um, 1200 bar
Total Choices >140 >70
Bonded Phases 15 14
Lengths 150, 100, 75, 50, 30, 20mm 150, 100, 75, 50mm
Column ID’s 2.1, 3.0, 4.6mm 2.1 and 3.0mm, ,
Pore Sizes 80Å, 95Å, 80Å, 95Å, 300Å
Scalability to other particle sizes
Yes – 3.5, 5, 7um Yes – 3.5, 5, 7um
Recommended Choice for LC
Eclipse Plus C18, 4.6 x 50, 1.8um 959941-902
Eclipse Plus C18, 3.0 x 100, 1.8um 959758-302
Recommended Choice for LC/MS
Eclipse Plus C182.1 x 50mm, 1.8um959741-902
Eclipse Plus C182.1 x 100mm, 1.8um959758-902
Rapid Resolution HT Columns: Up to 20x Faster
Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over)• Compliant with strictest regulatory performance requirements
HPLC, 40°CSB-C184.6 x 150mm, 5µm1.20ml/min, 40°CAnalysis Time = 11minAnalysis Time = 11min
min0 2 4 6 8 10
RRHT SB-C182.1mm x 50mm 1.8µm RRLC 40°C1.00ml/min, 40°CAnalysis Time= 1.1min
RRLC, 40 C10x faster
min0.2 0.4 0.6 0.8 10
RRHT SB-C182.1mm x 50mm 1.8µm2.40ml/min, 95°CAnalysis Time: 0.4min
RRLC, 95°C27x fasterPW = 197msec
0.2 0.60 0.4 min >20X faster
Reduce Analysis Times Dramatically
Long Term Stability at High Temp.
RR High DefinitionRR High Definition
What Can I do Beyond This with UHPLC?RRHD Columns for Use with the Infinity 1290 LC
Use a wider range of mobile phase choices
More viscous solvents – methanol, other alcoholsMobile phase additives to optimize separations (they increase viscosity)
Go faster – wider flow rate range!Go faster wider flow rate range!
Use longer columns – maximize resolution!
Optimize methods faster improve productivity!Optimize methods faster, improve productivity!
The 1290 Infinity Has a Wide Useable UHPLC Range
June 06, 2005
RRHD/RRHT Short 50mm Columns with Typical ACN Gradient Condition Don’t Require P > 600 barACN Gradient Condition Don t Require P 600 bar
Sample:Octyl dimethyl para-aminobenzoic acid, Column: RRHD Eclipse Plus C18 3 x 50mm 1.8um, Temp: 40C, Flow Rate: 1.5 ml/min, Gradient: 40-90% ACN/water over 2 minutes. Pressure: Up to 460 barPressure: Up to 460 bar
LC Beyond 600 bar Requires New RRHD ColumnsAgilent ZORBAX RRHT Eclipse Plus C18 2 1 x 100 mm 1 8 um; PN 959764‐902Agilent ZORBAX RRHT Eclipse Plus C18, 2.1 x 100 mm, 1.8 um; PN 959764‐902,
SN USUXU01664, LN B08101
1817 7 21 259
700
y = 935.21x + 7.1903
R2 = 0.9993
y = 1025.3x + 13.279
R2 = 0.9973
y = 1817.7x + 21.259
R2 = 0.9946
500
600
300
400
Pressure (b
ar)
Acetonitrile/Water(60:40)
200
300
Acetonitrile/Water(50:50)
Methanol/Water(60:40)
0
100
0 0.1 0.2 0.3 0.4 0.5 0.6
Flow Rate (mL/min)
Rapid Resolution High Definition
Parameter RRHD1.8um, 1200 bar
RRHT 1.8um, 600bar
Total Choices >70 >140
Bonded Phases 14 15
Lengths 150, 100, 75, 50mm 150, 100, 75, 50, 30,20mm
Column ID’s 2.1 and 3.0mm 2.1, 3.0, 4.6mm
Pore Sizes 80Å, 95Å, 300Å 80Å, 95Å,
Scalability to other Yes – 3.5, 5, 7um Yes – 3.5, 5, 7umyparticle sizesRecommended Choice for LC
Eclipse Plus C18, 3.0 x 100, 1.8um 959758-302
Eclipse Plus C18, 4.6 x 50, 1.8um 959941-902302 902
Recommended Choice for LC/MS
Eclipse Plus C182.1 x 100mm, 1.8um959758-902
Eclipse Plus C182.1 x 50mm, 1.8um959741-902
Fast Separation with High Resolution on RRHD Column and Agilent 1290 Infinity LCColumn and Agilent 1290 Infinity LC
O t l di th l i b i idOctyl dimethyl para-aminobenzoic acid, RRHD Eclipse Plus C18, 2.1 x 100mm 1.8um, 40°C, 0 74 l/ i0.74 ml/min, 40-90% ACN/water over 4 minutes. Up to 775 bar
May 2009
Methanol Selectivity Can Be Explored When Operating Pressure is not a ProblemWhen Operating Pressure is not a Problem
Octyl dimethyl para-aminobenzoic acid, y y pRRHD Eclipse Plus C182.1 x 100mm 1.8um, 40C, 0.52 ml/min, 50-100% MeOH/water over 5.7 minutes. Up to 845 bar
Increase Flow for Fastest Separation on RRHD Use Complete Pressure Range of 1290 Infinity UHPLC
F = 2 ml/min T = 60°CF = 2 ml/minP = 975 bar
0.5min
mAU
300
400
500
T 60 CV_i = 0.5 µl x 100ppm eachWL = 275 nm DR = 160 Hz
min0 0 05 0 1 0 15 0 2 0 25 0 3 0 35 0 4 0 450
100
200
F = 2.3 ml/minP = 1110 bar 0.4min
ZORBAX RRHD SB-C18 2 1 x 50mm 1 8 µm
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45mAU
300
400
500
2.1 x 50mm 1.8 µm1200 bar
min0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.450
100
200
Maximizing Productivity by Maximizing Resolution
• Sometimes resolution is more important than speed!
• UHPLC offers many alternatives for increasing resolutionUHPLC offers many alternatives for increasing resolution.
• Speed can be maintained for some separations – will depend on sample complexity, flow rate etc.
Choose Column Length for Analysis Time and RsMobile Phase: 40:60 water:acetonitrile, Flow Rate: 0.5 mL/min, Temp: ambient, Columns: RRHD SB-C18, 2.1 mm ID, L th t d P k 1 il 2 t h 3 i h 4 b t h 5 l h 6 h h
RRHD 1.8um 1200 bar
Lengths as noted Peaks: 1: uracil, 2 acetophenone, 3 propiophenone, 4 butyrophenone, 5 valerophenone, 6 hexanophenone
Rs3,2: 5.64N6 : 10,700N6 : 10,700Pressure: 350 bar50 mm
min0 1 2 3 4
Rs3,2: 9.02N6 : 21,100Pressure: 566 bar
100 mm
Rs3,2: 11.98N6 : 34,100150 mm
min0 1 2 3 4
Pressure:750 bar
min0 1 2 3 40
The 150mm length RRHD column becomes a good option for high resolution with g g p gthe 1290 Infinity LC.
Gain Efficiency and Resolution with Long Length 150 + 100 mm = 250 mm 1.8um @ 1100 bar150 100 mm 250 mm 1.8um @ 1100 bar
α3,2: 1.196 42
150 mm (P:582 bar)
Mobile Phase 25:75 water:acetonitrile, Flow Rate: 0.5 mL/min, ambient temperature, ZORBAX RRHD SB-C18, 1.8um columns
mAU300
Rs3,2: 6.42N6 : 31,400Pw2: 0.016 minPw6: 0.029 min
50 ( 58 ba )
100
150
200
250
α3,2: 1.20Rs3 2: 8.76
150 +100 mm (P:1095 bar)1. Uracil2 Acetophenone
min0 0.5 1 1.5 2 2.5 3 3.5
0
50
mAU
250
300
Rs3,2: 8.76N6 : 50,200Pw2: 0.018 minPw6: 0.037 min
2. Acetophenone3. Propiophenone4. Butyrophenone5. Valerophenone6. Hexanophenone
50
100
150
200
250
High Rs in 3.5 minutes, 250mm, 1.8um at 1100 bar
min0 0.5 1 1.5 2 2.5 3 3.5
0
50
Resolution is fine on 150mm column, but for even more Rs longer column is used.
Where Does this Example Fall in the 1290 Infinity Range
This app falls in the “Power Range”pp g
Superficially Porous ColumnsSuperficially Porous Columns
Instrument Evolution
Legacy (<400bar)
• 3.5 and 5 micron
Modern UHPLC (600-1200 bar)
• Sub 2 micron
Method Transfer
Change in Column
SOP/ Dual Methods? €, $, £SOP/ Dual Methods? , $,
Poroshell 120 Columns for HPLC and UHPLC
• 80-90% efficiency of sub 2um
Poroshell 120 columns have:
• ~40-50% lower pressure
• 2.7um particle sizep
• 2um frit to reduce clogging
• 600 bar pressure limit• 600 bar pressure limit
The Poroshell particle has a solid core (1.7um) and a porous outer layer with a 0.5um diffusion path
Efficiency vs. 1.8μm
Comparable base loading to totally porous LC
What can be achieved?What can be achieved?
Comparison of 4.6 x 250 mm 5 um to Poroshell 120 EC-C18 4.6 x 100 mm, 2.7um
mAU100
11.5
96
Sulfadiazine,Sulfathiazole SulfapyridineSulfamerazine,Sulfamethazine, Sulfamethazole, Sulfamethoxypyridazine
Time %B0 833 3334 33Column: Eclipse Plus C18
Mobile Phase:A: 0.1% formic acid in WaterB: 0.1% formic acid in ACN
Time %B0 812 3313.2 33Column: 4.6 x 100mmPoroshell 120 EC C18
40
60
80
100
9.71
2
11.1
16
12.6
74
15.2
48
16.1
5116
.435
20.6
87
23.0
76
29.2
90
110 bar
Sulfamethoxypyridazine,SulfachloropyridazineSulfamethoxazole, Sulfadimethoxine
4.6 x 250mm, 5umFlow Rate: 1 mL/min
Poroshell 120 EC-C18, 2.7umFlow Rate: 1 mL/min
min5 10 15 20 25 300
20
mAU 7.03
7
32 b
200
250
1.71
99
2.31
12.
606
7 4.5
58
5.45
00
• Faster analysis (shorter column higher flow rate)• More Sensitive (smaller particle sharpens peak)• Better Optimized (faster analysis allows better tweaking)
325 bar
0
50
100
150 2.18
9
3.86
7
4.43
7
5.92
0
• Both run on Agilent 1100 G1315B DAD (under 400 bar)• No change in sample prep required, 2um frit used on both columns
min5 10 15 20 25 30
0
Comparison of 4.6 x 250 mm 5 um to Poroshell 120 EC-C18 4.6 x 100 mm, 2.7um
mAU100
11.5
96
See conditions previous slide
40
60
80
100
9.71
2
11.1
16
12.6
74
15.2
48
16.1
5116
.435
20.6
87
23.0
76
29.2
90
110 bar
min5 10 15 20 25 300
20
mAU
1 7.03
7
100
150
200
250
1.71
9
2.18
92.
31
2.60
6
3.86
7
4.43
7 4.55
8
5.45
0
5.92
0
325 bar
5
0
50
100 3
min
Best format for <400bar Instrument Range
USP Method Naproxen
USP Method Transfer: Simvastatin
PoroShell has 600bar Working Range!UHPLC S ti ith hi h i it l tUHPLC Separations with higher viscosity solvents
Growing PoroShell Range
Parameter RRHD1.8um, 1200 bar
RRHT 1.8um, 600bar
Poroshell 1202.7um 600,bar
Total Choices >70 >140 >80
Bonded Phases 14 15 8
Lengths 150, 100, 75, 50mm 150, 100, 75, 50, 150, 100, 75, 50, 30, 20mm 30mm
Column ID’s 2.1 and 3.0mm 2.1, 3.0, 4.6mm 2.1, 3.0, 4.6mm
Pore Sizes 80Å, 95Å, 300Å 80Å, 95Å, 120Å
Scalability to otherparticle sizes
Yes – 3.5, 5, 7um Yes – 3.5, 5, 7um Nearly identical selectivity to 3.5, 5um
RecommendedChoice for LC
Eclipse Plus C18, 3 0 100 1 8 m
Eclipse Plus C18, 4 6 50 1 8 m
Poroshell 120 EC-C184 6 100mm 2 7 mChoice for LC 3.0 x 100, 1.8um
959758-3024.6 x 50, 1.8um 959941-902
4.6 x 100mm, 2.7um695975-902
RecommendedChoice for LC/MS
Eclipse Plus C182.1 x 100mm, 1.8um
Eclipse Plus C182.1 x 50mm, 1.8um
Poroshell 120 EC-C183.0 x 100mm, 2.7um
959758-902 959741-902 695975-302
New Bonded Phases on Poroshell 120 O• Utilizes the same Eclipse Plus bonding process utilized in ZORBAX
Eclipse Plus Phenyl-Hexyl• Excellent choice for resolution of analytes such as aromatic
compounds, due to unique pi-pi interactions, or when other phenyl type columns are recommended
Poroshell 120 Phenyl Hexyl
• Embedded polar group provides unique selectivity for polar compounds• Often changes elution order of acidic and basic compounds• Stability from pH 2-9 allows for flexible method development across a
id H
Poroshell 120 Bonus-RP
wide pH range
• Proprietary bonded phase is an excellent choice for polar analytes that don’t retain well on C18StableBond chemistry enhances low pH stability
Poroshell 120 SB-AQ • StableBond chemistry enhances low-pH stability
• Good choice for polars where 100% aqueous mobile phase is needed. SB-AQ
• Bare silica HILIC (ships in HILIC solvents) for separations of polar dPoroshell 120 compounds
• Ideal for retention and analysis of polar compounds by LC/MS in high organic for maximum sensitivity
Poroshell 120 HILIC
These two bonded phases are for the analysis of very polar and polar basic compoundsThese two bonded phases are for the analysis of very polar and polar basic compounds.Use SB-AQ for LC-UV and HILIC for LC/MS
Comparison of Bonded Phases - 8 Steroids
mAU
50
100
150 Poroshell 120 EC-C18 13
2,5 6
4 78
0 2 4 6 8 10 12
0
mAU
50
100
150 Poroshell 120 SB-C18 13
2,5,64 7 8
0 2 4 6 8 10 12
0
mAU
50
100
150 Poroshell 120 Phenyl Hexyl1
3 7 842 5 6
0 2 4 6 8 10 12
0
mAU
50
100
150 Poroshell 120 Bonus RP 13,2 7,86,4
5
0 2 4 6 8 10 12
0
50
1.Hydrocortisone, 2.B Estradiole, 3. Andostadiene 3,17 dione, 4. Testosterone, 5. Ethyestradione 6 Estrone 7 Norethindone acetate 8 ProgestreoneEthyestradione, 6. Estrone, 7. Norethindone acetate, 8. Progestreone
Comparison of Bonded Phases - Beta Blockers
2mAU
100
200
300
1 34
5 6 7
Poroshell 120 EC-C18
min0 2 4 6 8 10 12-100
0
mAU
100
200
300
12
3 4 5 6 7Poroshell 120 SB-C18
min0 2 4 6 8 10 12-100
0
100
mAU
200
3002
3 45
Poroshell 120 Phenyl Hexyl
min0 2 4 6 8 10 12-100
0
100
200 1 3 4 7 6
mAU
3002
5 Poroshell 120 Bonus RP
min0 2 4 6 8 10 12-100
0
100
200 1 3 45
7 6Poroshell 120 Bonus RP
1 Atenolol 2 Pindolol 3 Naldolol 4 Metoprolol 5 Acebutolol 6 Propranolol 7 Alprenolol1.Atenolol, 2. Pindolol, 3. Naldolol, 4. Metoprolol, 5. Acebutolol, 6. Propranolol, 7. Alprenolol
Separation of Planar Azo-DyesAU
DAD1 A, Sig=260,80 Ref=off (C:\DOCUMEN...INGS\WILLLONG\MY DOCUMENTS\DEC14\DATA\AZO DYES\SURVEY30000002.D)
min0 1 2 3 4 5 6 7 8 9
mAU
0100200300400
DAD1 A Si 260 80 R f ff (C \DOCUMEN INGS\WILLLONG\MY DOCUMENTS\DEC14\DATA\AZO DYES\SURVEY30000004 D)
Poroshell 120 EC‐C18
mAU
0100200300400
DAD1 A, Sig=260,80 Ref=off (C:\DOCUMEN...INGS\WILLLONG\MY DOCUMENTS\DEC14\DATA\AZO DYES\SURVEY30000004.D)
Poroshell 120 SB‐C18
min0 1 2 3 4 5 6 7 8 9
mAU
100200300400
DAD1 A, Sig=260,80 Ref=off (C:\DOCUMEN...INGS\WILLLONG\MY DOCUMENTS\DEC14\DATA\AZO DYES\SURVEY30000006.D)
Poroshell 120 Phenyl Hexyl
min0 1 2 3 4 5 6 7 8 90
mAU
200300400
DAD1 A, Sig=260,80 Ref=off (C:\DOCUMEN...INGS\WILLLONG\MY DOCUMENTS\DEC14\DATA\AZO DYES\SURVEY30000008.D)
Poroshell 120 Bonus RP
15 to 100 %MeOH over 10 min @ 0 4 ml/min SolventA is 10 mM AmmoniumAcetate pH 4 8
min0 1 2 3 4 5 6 7 8 90
100200
15 to 100 % MeOH over 10 min @ 0.4 ml/min, Solvent A is 10 mM Ammonium Acetate pH 4.8
PoroShell Summary
• Extraordinary lot-to-lot reproducibility – Poroshell 120 columns are manufactured using a proprietary single-step porous shell process that dramatically reduces
minute differences between columns and lots
• Comparable speed and resolution to sub-2 μm columns- with up to 50% less backpressure – take HPLC and UHPLC performance to a new level of fl exibility and efficiency
• Superior peak shape – especially at pH 6-7 – for faster, more accurate results
• Long column life – Poroshell 120 columns use a standard 2 μm frit, and resist plugging with dirty samples
• Multiple bonded phases – C18s, C8s, Phenyl-Hexyl, and more! A growing family of bonded phases Multiple bonded phases C18s, C8s, Phenyl Hexyl, and more! A growing family of bonded phases for method development flexibility.
• Easy method transfer and scalability to ZORBAX bonded phases, for highest
productivity from lab to lab around the worldproductivity from lab to lab, around the world
• UHPLC guard column options that reduce your operating expenses by extending
the life of Poroshell 120 columns
Three Key Resources for Small Molecules Analysis
The LC Handbook5990-7595EN
LC Column Selection Guide5990-4435ENTo be updated 2012
LC Columns Poster5990-5325EN
5990 7595EN To be updated 2012
And the Newest On-Line Tool “The Navigator”: A Column and Sample Prep Selection ToolColumn and Sample Prep Selection Tool
“The Navigator” Can Provide Recommendations Based on the Users Best Starting Point
Get help and tech support will go to a special page to help customers get
Based on the Users Best Starting Point
immediate help in their language. They’ll also be prompted to provide feedback about the tooltool.
The Navigator provides recommendations based on information the chromatographer has at hand.
http://navigator chem agilent com OR: www agilent com/chem/navigatorhttp://navigator.chem.agilent.com OR: www.agilent.com/chem/navigator
Three Key Resources for Bio Molecules AnalysisHighlight Complete Range of Columns andHighlight Complete Range of Columns and
Recombinant Protein Characterization Guide5990-8566EN
Bio Columns Poster5990-5526EN Bio Columns Guide
5990-9384EN5990-8566EN
Method Translation Tool
Thank You for your Attention!
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