Postdoctoral Trainee Exchange Program between UTSW and the First Affiliated Hospital of Sun Yat-‐sen University
Trainee: Xiuyu Leng
Mentor: Jay W. Schneider
UTSW, Dallas, Feb 2013
Isoxazole (ISX) !Cardio-regenerative
small-molecules!
Lab Research Focus:
UTSW chemical library
Project 1. ISX and fibrosis
Results: Isx a;enuates pro-‐fibro@c changes induced by Ang II in vivo.
. ACTA2 COL DAPI
QuesUon: Can ISX reduce Ang II induced fibrosis?
Ang II
Project 2. Studies of heart repair by magne@c resonance imaging (MRI)
Pre-‐ treat with ISX or VEH for 14 days
D0 D3 D7 D14
D1, Injury (MI)
Cardiac MRI
Treat with ISX or VEH for totally 28 days
Before MI AVer MI
Study design:
Project 3. Studies of GPR68
GPR68 ACTN2 DAPI
FLAG TOPONINI DAPI
TransducUon of GPR68 adenovirus in neonatal rat cardiomyocytes
GPR68 and ACTN staining in mouse heart
Project 4. ISX and GPR68 in neurogenesis
Coupling Hippocampal neurogenesis to brain PH through proneurogenic small molecules that regulate proton sensing G protein-coupled receptors.
ACS Chemical Neuroscience. 2012.
TransducUon of GPR 68 adenovirus in HCN cells
FLAG TUJ1 DAPI
• Acquired exper@se in lab techniques: – Cell culture, RT-‐PCR, immunocytochemistry, protein blo^ng and perfusion
fixa@on of hearts, etc.
• Addi@onal exci@ng technologies: – Microarray, proteomics, NMR(Nuclear magne@c resonance spectroscopy), etc
• Observa@on of pharmacological regula@on of heart repair following injury
• Co-‐authored one paper • Next paper in prepara@on
– (A cardiogenic small-‐molecule that regulates myocardial metabolism, heart func@on and survival by Schneider et al)
• Contribu@on of GPR68 adenovirus data to future papers.
Summary of two years’ training in UTSW