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UTC-IACUC PROTOCOL FORM
(FOR USE OF LIVE VERTEBRATE ANIMALS)
Submit the electronic version of the protocol to: [email protected]
Animals may not be procured and research involving animals may not begin until the PI is notified in
writing that the protocol has been approved by the IACUC.
Principal Investigator/Instructor(s): Department: Click here to enter text. Click here to enter text.
Telephone Numbers:
Office: Cell: E-Mail: Click here to enter text. Click here to enter text. Click here to enter text.
Alternate for animal emergency or study-related action/communication with Authority to act in the
Investigator’s absence: Click here to enter text.
Alternate’s Telephone Numbers:
Office: Cell: E-Mail: Click here to enter text. Click here to enter text. Click here to enter text.
Title of Project /Course: (If external funding source is listed in section B of this protocol, title of this
protocol should match the title of application to the sponsor.) Click or tap here to enter text.
☐ I have read and agree to follow the IACUC Bylaws and SOPs #1- 6 (www.utc.edu/research-integrity/institutional-animal-
care-use-committee/policy-and-guidelines.php)
Your signature as P.I., Co-investigator, or Department Head on this application verifies that: (1) the information herein is true and
correct and that you are familiar with and will comply with the legal standards of animal care and use established under federal and
state laws and policies as well as university policies; (2) the proposal has received approval for scientific and/or educational merit by
peer review; and (3) the activities do not unnecessarily duplicate previous experiments.
Signature of PI/Instructor Date
Signature of Co-PI/Instructor(s) Date
Signature of Department Head (Dean/Associate Dean if Dept. Head is PI) Date
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1. GENERAL INFORMATION
A. This protocol:
Anticipated Start Date: Click here to enter text. End Date: Click here to enter text.
These dates must be later than the IACUC meetings. Best practice is to place your start date at least two weeks
following the third Tuesday of each month.
☐ New ☐ 3-Year Rewrite (Please provide a brief description of research progress to date.)
Click here to enter text.
☐ Holding ☐ Breeding ☐ Research ☐ Teaching (Course Name: Click here to enter text.)
☐ Collaboration between UTC and any another institution/agency/outside entity. A memorandum of
understanding (MOU) must be submitted prior to IACUC protocol review and commencement of the work
or movement of animals. If working with another institution, please provide the context of the collaboration
(i.e., funding, personnel involvement, animals). Click or tap here to enter text.
☐ Part of a grant or other contractual obligation:
Please provide grant/contract information Click here to enter text.
B. Name and address of veterinarian responsible for clinical care:
Click here to enter text.
C. Funding Source for this protocol (internal-external grants and other sources):
Click here to enter text.
D. Non-Technical Summary
A complete non-technical summary is required for conformance with Public Health Service Policy. Non-technical
summary must be submitted as an attachment and should not exceed two (2) pages. Non-technical summary must be
written to ensure comprehension by non-scientists on an 8th grade level. It must contain the following:
1) Objectives of the research or teaching activity;
2) Species and number of animals;
3) Methods used to reduce any experimental stress or discomfort
4) Schedule of the course or the study procedures performed on animals during each phase
5) Benefits, outcome and results expected in non-technical terms.
E. Flow Chart
Attach as a separate document a flow chart or bulleted list that depicts the sequence of all animal procedures or
manipulations (step by step) to be performed in this protocol.
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2. ANIMAL WELFARE
A. Benefit
Describe the potential scientific benefit of the proposed study. Be convincing as to why this work is
important for advancement of knowledge, improving human or animal health, or for the good of society.
Click here to enter text.
B. Rationale
Please explain your rationale for using live animals and why the proposed species is/are the most appropriate
for this study.
Click here to enter text.
C. Duplication
The Animal Welfare Act and USDA Animal Care Policy #12 require PIs to assure the IACUC that you have
considered whether or not your proposed work unnecessarily duplicates existing knowledge.
Does the proposed activity unnecessarily duplicate any previous work? ☐ YES ☐ NO
(Note: Teaching activities are duplicative but instruction of new students warrants repeat of these activities. It is necessary that a robust
alternatives search be performed for ethical category “D” teaching protocols and very briefly include the pertinent alternatives information
being done prior to live animal use. It should state, for example, that videos were watched, simulation labs were done, “dummy models were
worked on and that the students are prepared to progress to live animals.)
If yes, please justify.
Click here to enter text.
D. Search for Alternatives to Painful/Distressful Procedures
A painful procedure in an animal is defined as any procedure that would be reasonably expected to
cause more than slight or momentary pain and/or distress in a human. The UTC-IACUC is responsible
for ensuring that investigators have appropriately considered alternatives.
Does this study/activity include painful or distressful procedures? ☐ YES ☐ NO
If no, skip to Section E. If yes, the minimal written narrative should include:
a. The databases searched or other sources consulted,
b. The date of the search and the years covered by the search,
c. Key words and/or search strategy used,
d. A narrative written in such a way that the IACUC can readily assess whether the search topics were
appropriate and whether the search was sufficiently thorough.
Click here to enter text.
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For information on conducting an alternatives search and appropriate databases, please visit:
http://www.nal.usda.gov/awic/alternatives
Please Note: Google Scholar IS NOT A RESEARCH DATABASE AND CANNOT BE USED AS A RESOURCE
FOR ALTERNATIVE SEARCHES.
Literature searches for alternatives should be performed specifically for those proposed procedures that may cause
more than slight or momentary pain and/or distress.
Alternatives or alternative methods are generally regarded as those that incorporate some aspect of replacement,
reduction, or refinement of animal use in pursuit of the minimization of animal pain and distress consistent with the
goals of the research. To satisfy this requirement, the USDA believes that database searches remain the most
effective and efficient method for demonstrating compliance with the requirement to consider alternatives to
more than momentary painful / distressful procedures.
For each painful procedure, were alternatives identified (to replace, refine, and reduce)?
Please address appropriate answer(s) below if yes or no. ☐ YES ☐ NO
If “Yes”, please describe:
Click here to enter text.
Will these alternatives be incorporated into your proposed work? ☐ YES ☐ NO
If not, please explain?
Click here to enter text.
If “No”, please provide a written narrative about your search results including references:
Click here to enter text.
E. Monitoring Health and Well-being
Animals should be monitored at regular intervals for health and well-being. The frequency of this monitoring by
personnel listed on the protocol is variable and based on the protocol’s procedures.
Database Information
Name of Database searched * Click here to enter text.
Date of search (must be within 6 months of
protocol submission) MM/DD/YY
Click here to enter text.
Years covered by search (YY – YY) Click here to enter text.
Search strategy (must show how keywords were
combined)
Click here to enter text.
Other sources consulted. Provide individuals’
name, qualifications, date and summarize content
Click here to enter text.
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Health Assessment
How often and by whom will the clinical condition of animals be monitored?
Click here to enter text.
What are the anticipated health changes?
Click here to enter text.
What methods will be used to monitor health changes?
Click here to enter text.
The individuals responsible for monitoring the health and well-being of your protocol animals are: (provide
contact information including cell phone number and email address:
Click here to enter text.
F. Removal from study
If necessary, what criteria will be used to remove an animal from use prior to the planned conclusion of the study?
Include what “remove from study” means. For example, will animal no longer participate in the study, but be returned
to colony/herd or will animal be euthanized immediately? For each criterion, define a specified duration or
endpoint. For example, “animals will be removed from the study if diarrhea is observed for greater than 24 hours”.
Some studies would have very limited removal criteria if they are short term – possibly if the animal is fractious or
have a low blood count when pre-study blood work is performed. Other criteria would be directly related to the
specific study/activity procedures.
Click here to enter text.
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3. ANIMAL DESCRIPTION, HOUSING AND USE LOCATION(S)
A. Description of Animals
Protocols are approved for three years; therefore, the number of animals requested should be the total
number of animals needed for the entire project over the three-year period.
Species: Common name Species: Scientific name Source Sex Total # Animals
Requested
Maximum
# per day
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MORE LINES AS NEEDED
List strains of rodents whose use is anticipated. Exact numbers of each strain is not warranted. This
information may be beneficial for husbandry purposes.
Click here to enter text.
B. Animal Number Justification. Provide an explanation of how animal numbers were derived and justify
that need. A table may help clarify different experimental groups or studies and the specific numbers needed
for each.
Click here to enter text.
C. Animal Housing and Use Areas
Please check your preferred housing location. Animal housing space is assigned by the IACUC. Every reasonable
attempt will be made to accommodate investigator requests for preferred housing locations.
☐ Davenport Hall
☐ Holt Hall
☐ UTC-IACUC Approved Satellite Facility
List Bldg./Room #:
Click here to enter text.
☐ Other. Please describe:
Click here to enter text.
D. Are there any special requirements for housing/husbandry? ☐ YES ☐ NO
If “YES”, please complete:
☐ Sterile Cages
☐ Wire Bottom Cages*
☐ Individually Housed*
☐ Other. Please describe:
Click here to enter text.
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*Please provide scientific justification for use of this type of housing/husbandry:
Click here to enter text.
E. Will animals be removed from the animal housing location? ☐ YES ☐ NO
If yes, please answer the following:
1. Animals will be taken to (bldg/room number): Click here to enter text.
2. Animal manipulations that will be performed in the laboratory include:
Click here to enter text.
3. Estimated total time period live animals will be kept in the laboratory: Click here to enter text. hours.
4. Will animals be returned to the animal housing facility? ☐ YES ☐ NO
F. Planned disposition of the animals after completion of the activity:
☐ Return to production/breeding unit/facility inventory
☐ Slaughter, (must conform to the Humane Slaughter of Livestock, 9 CFR, part 313)
☐ Returned to owner
☐ Transfer to another research project (if yes, project title and investigator)
☐ Sold
☐ Euthanized (Fill out section on Euthanasia)
☐ Other. Please describe:
Click here to enter text.
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4. PERSONNEL
List all personnel involved in this protocol and provide training information. Include all investigators, student
employees, post-doctoral researchers, staff research associates and laboratory assistants. Personnel or groups of
personnel listed will be the only personnel approved to work under this protocol. Should new staff need to be added,
this must be done via an amendment, which must be approved before the new staff may begin working with the
animals.
1CITI Training information: Documentation that all personnel are appropriately trained in animal care and use is federally and
institutionally mandated. A general web-based training and certification program is required for all personnel. Further, more
specific training may be necessary depending on the species and procedures proposed. Please review the UTC IACUC website
for training requirements and resources under “Training” at: http://www.utc.edu/research-integrity/institutional-animal-care-use-
committee/training.php .
Name + Degree+ email
address
CITI completion date or
date of most recent
mandatory training1. This
must be completed prior to
working with animals.
Procedures this person will perform & relevant
experience/training
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5. ANIMAL PROCEDURES
Please check all the listed procedures that apply to your protocol and fill out the appropriate sections.
☐ A. Euthanasia
☐ B. Behavioral studies.
☐ C. Animal field studies.
☐ D. Off-site captive animal studies.
☐ E. Anesthesia/analgesia.
☐ F. Surgical Procedures.
☐ G. Projects involving captive aquatic animals.
☐ H. Hazardous agent use (biological, radioactive, or chemical).
☐ I. Use of Recombinant Materials.
☐ J. Substance(s) collected from live animals (Blood*, or other body fluid withdrawal, tissue collection, tail clip*)
(Do not list collections post mortem)?
☐ K. Other manipulations not described above.
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A. EUTHANASIA
Information must be provided for all live animal activities, including non-terminal studies, where an animal may
experience a Humane Endpoint not related to the research. Methods of euthanasia must be acceptable by the most
recent Report of the AVMA Guidelines on Euthanasia, which can be found at the following web address:
https://www.avma.org/KB/Policies/Documents/euthanasia.pdf. Conscious physical methods must be scientifically
justified below. All agents used for the euthanasia of animals must be in-date and approved through IACUC.
☐ Euthanasia is part of the study design
OR
☐ Euthanasia is NOT part of the study design
Method of euthanasia:
☐ Anesthetic Barbiturate Overdose (An approved veterinary product):
Click here to enter text.
☐ Anesthetic overdose
Drug:
Click here to enter text.
Dose:
Click here to enter text.
Route:
Click here to enter text.
☐ Blunt Force Trauma. Justification:
Click here to enter text.
☐ Cervical Dislocation (CD) under anesthesia or tranquilization
☐ Conscious Cervical Dislocation. Justification:
Click here to enter text.
☐ Conscious Decapitation. Justification:
Click here to enter text.
☐ Decapitation under anesthesia or tranquilization
☐ Exsanguination following anesthesia, stunning, captive bolt or CO2
Is Perfusion involved?
☐ NO
☐ YES. Please provide details:
Click here to enter text.
☐ Slaughter (covered under the Humane Slaughter of Livestock, 9 CFR, Part 313)
☐ Other. Specify:
Click here to enter text.
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B. BEHAVIORAL STUDIES
1. Describe animal methodology/test(s) to be used:
Click here to enter text.
2. Intensity and duration of stimulus:
Click here to enter text.
3. Frequency of tests:
Click here to enter text.
4. Length of time in test apparatus:
Click here to enter text.
5. Potential adverse events:
Click here to enter text.
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C. ANIMAL FIELD STUDIES
1. List and include a copy of any permits required (e.g. Tennessee Wildlife Resources Agency, U.S. Fish and Wildlife
Service, Endangered Species)
Name and number:
Click here to enter text.
2. Study Site(s)
General Location:
Click here to enter text.
3. Describe methods employed to prevent potential disease transmission between individual animals encountered
in this activity (Use of disposable gloves; disinfect gloves between animals):
Click here to enter text.
4. Describe methods employed to prevent potential disease transmission across multiple sites in this activity (Use
of disposable gloves; disinfect gloves between animals):
Click here to enter text.
5. Capture with mechanical devices
a. Type/description of capture device/method:
Click here to enter text.
b. Frequency of checking capture device:
Click here to enter text.
c. Maximum time animal will be in capture device:
Click here to enter text.
d. Methods to ensure well-being of animals in capture device:
Click here to enter text.
e. Methods to avoid non-target species capture:
Click here to enter text.
f. Expected injury and/or mortality rates:
Click here to enter text.
g. Animal will be released at capture site after completion of procedures: ☐ YES ☐ NO
If no explain:
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Click here to enter text.
h. Precautions used to minimize injury and/or mortality?
Click here to enter text.
i. Describe method(s) used to sanitize capture devices between capture locations or groups of individuals:
Click here to enter text.
6. Capture with chemical immobilization.
a. Type of dart or device to administer drugs:
Click here to enter text.
b. Method of dart propulsion:
Click here to enter text.
c. Precautions used to minimize injury and/or mortality:
Click here to enter text.
d. If wild animals will be anesthetized and released to the wild, describe procedures for assuring that animals are
sufficiently recovered from anesthetic to be released. Consider that prey species may have to be monitored until
fully recovered to avoid predation.
Click here to enter text.
7. Marking/Telemetry Procedures
a. Describe marking procedures to be used:
Click here to enter text.
b. If a telemetry package is to be attached, describe:
1. Weight of the total package
Click here to enter text.
2. Type of antenna (including length)
Click here to enter text.
3. Method of attachment
Click here to enter text.
4. Will marking/telemetry device be removed? ☐ YES ☐ NO
If yes, explain how:
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Click here to enter text.
8. Release of animals other than at capture site (for non-survival collection please see #9)
a. Where will captured animals be released?
Click here to enter text.
b. If the animals are transported indicate the method of transportation:
Click here to enter text.
c. If the animals are to be housed, fill out Animal Housing and Use Location(s) section.
Click here to enter text.
d. If release is not the general location of capture, justify.
Click here to enter text.
9. Non-Survival Collection
a. Describe procedure(s) to be used:
Click here to enter text.
b. Describe precautions that will be taken to prevent non-target mortalities:
Click here to enter text.
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D. OFF-SITE CAPTIVE ANIMAL STUDIES
1. List and include a copy of any permits required (e.g. Tennessee Wildlife Resources Agency, U.S. Fish and Wildlife
Service, Endangered Species) as well as the required Memorandum of Agreement (MOA) between the
researcher, and the owner/operator of the sites involved (e.g. zoos, organizations, private entities, others)
Name and number:
Click here to enter text.
2. Study Site(s)
General Location:
Click here to enter text.
3. Describe methods employed to prevent potential disease transmission between individual animals encountered
in this activity (Use of disposable gloves; disinfect gloves between animals):
Click here to enter text.
4. Describe methods employed to prevent potential disease transmission across multiple sites in this activity (Use
of disposable gloves; disinfect gloves between animals):
Click here to enter text.
5. Describe responsibilities of the researchers and owner/operator of the sites:
a. Type/description of researcher responsibilities:
Click here to enter text.
b. Type/description of owner/operator of the sites responsibilities:
Click here to enter text.
c. Precautions used to minimize injury and/or mortality?
Click here to enter text.
6. Capture/release of animals:
a. If animals are handled, who will and how will they be captured?
Click here to enter text.
b. Precautions used to minimize injury and/or mortality:
Click here to enter text.
a. How will they be reintroduced to their environment at release:
Click here to enter text.
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E. ANESTHESIA/SEDATION/ANALGESIA
Adequate records describing anesthetic monitoring and recovery must be maintained and available in the animal’s medical
record. Animals must be monitored until awake and can maintain sternal recumbency.
Investigators are required to use pharmaceutical grade drugs and compounds whenever commercially available, even in
terminal procedures. All anesthetics and analgesics must be in-date, even for use in terminal procedures.
☐ For Clinical Trial Studies (mark if applicable), “The protocol will be determined on a patient by patient basis by the
duty anesthesiologist consistent with the standard of care for the veterinary profession using commercially available
products.”
Agent Concentration/
Dose (mg/kg) Volume and route Frequency
Pre-emptive analgesic Click here to enter text. Click here to enter
text.
Click here to enter
text.
Click here to
enter text.
Pre-anesthetic/sedation Click here to enter text. Click here to enter
text. Click here to enter
text. Click here to
enter text.
Anesthetic Click here to enter text. Click here to enter
text. Click here to enter
text. Click here to
enter text.
Post-procedural
analgesics*
Click here to enter text. Click here to enter
text.
Click here to enter
text.
Click here to
enter text.
Paralytics (need to justify
below in question h.)
Click here to enter text. Click here to enter
text.
Click here to enter
text.
Click here to
enter text.
Other (supplementary
drugs or compounds, i.e.
Atropine)
Click here to enter text. Click here to enter
text.
Click here to enter
text.
Click here to
enter text.
1. List each non-pharmaceutical drug, justifications for use, why pharmaceutical grade cannot be used (if applicable),
the preparation of the agent, labeling, and storage:
Click here to enter text.
If post-operative analgesics are going to be given as needed, an IACUC approved procedure on recognizing pain
and distress must be followed.
2. Reason for administering agent(s):
Click here to enter text.
3. For which procedures:
Click here to enter text.
4. Method and frequency of monitoring anesthetic depth during the procedure, including when paralyzing drugs are
used:
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Click here to enter text.
5. Methods of physiologic support during anesthesia and recovery:
Click here to enter text.
6. Frequency of recovery monitoring:
Click here to enter text.
7. Specifically what will be monitored:
Click here to enter text.
8. Describe any behavioral or husbandry manipulations that will be used to alleviate pain, distress, and/or discomfort:
Click here to enter text.
9. If using paralytic drugs, scientific justification is needed:
Click here to enter text.
10. Potential adverse events:
Click here to enter text.
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F. SURGICAL PROCEDURES
• All survival surgical procedures must be done aseptically, regardless of species or location of surgery.
• Adequate records describing surgical procedures, anesthetic monitoring and postoperative care must be maintained
and available for the attending veterinarian and animal care staff.
1. Location (Room/Building) of surgery:
Click here to enter text.
2. Type of surgery:
☐ Non-survival surgery: (animals euthanized without regaining consciousness).
☐ Major survival surgery: (major surgery penetrates and exposes a body cavity or produces substantial impairment of
physical or physiologic function).
☐ Minor survival surgery.
☐ Multiple major survival surgery.
If multiple major survival surgery, provide justification for multiple major survival surgical procedures:
Click here to enter text.
3. Pre-op preparation of the animals:
a. Food restricted for Click here to enter text. hours.
b. Water restricted for Click here to enter text. hours.
4. Surgery techniques (check all that apply):
☐ Survival surgery or procedures requires at a minimum, the use of sterile instruments, surgeon washing hands
prior to donning sterile gloves, mask, approved operating area, removal of hair or feathers, skin prep with
disinfectant like betadine, and aseptic techniques during the surgery or procedure.
☐ Non-survival surgery requires at a minimum the surgical site should be clipped, surgeon wears gloves, the
instruments and surrounding area is clean and approved as an operating area.
5. Describe the following surgical procedures:
a. Skin incision size and location on the animal:
Click here to enter text.
b. Describe surgery in detail (include size of implant if applicable):
Click here to enter text.
c. Method of skin closure (include number of layers, type of wound closure, proposed suture type, size ranges):
i. Type:
Click here to enter text.
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ii. Suture size/wound clips:
Click here to enter text.
iii. Pattern:
Click here to enter text.
6. Recovery from surgical manipulations:
a. Following recovery, what parameters will be monitored?
Click here to enter text.
b. Person who will monitor the animals?
Click here to enter text.
c. How frequently will animals be monitored?
Click here to enter text.
d. How long post-operatively will animals be monitored:
Click here to enter text.
e. Time post-operatively that sutures/staples will be removed, if applicable:
Click here to enter text.
f. What long-term side-effects resulting from the surgical procedure are expected during the course of the study and
how will these be monitored?
Click here to enter text.
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G. PROJECTS INVOLVING CAPTIVE AQUATIC ANIMALS
1. Water quality monitoring:
a. How is the water quality established/determined prior to introduction of animals?
Click here to enter text.
b. How is the water filtered to remove nitrogenous/animal waste compounds?
Click here to enter text.
c. How frequently and what parameters for the water quality are monitored?
Click here to enter text.
2. Housing:
a. Briefly describe the system design and housing used (include type of water circulation, tank size):
Click here to enter text.
b. Provide the approximate housing density:
Click here to enter text.
c. Provide how often and how the housing will be sanitized:
Click here to enter text.
d. What type of environmental enrichment is being provided in the tank/housing? (PVC pipe, plants, etc.). If none is
used, please justify:
Click here to enter text.
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H. HAZARDOUS AGENT USE (BIOLOGICAL, RADIOACTIVE, OR CHEMICAL)
Will any of the following be used in the animals either in the Animal Care Facility or elsewhere?
☐ * Infectious Agents
☐ * Toxic Chemicals or Carcinogens
☐ **Radioisotope
☐ *** Legend (prescription) drugs and/or controlled substances (SEE BELOW)
☐ Other. Please describe:
Click here to enter text.
If yes, list agent and describe all procedures necessary for personnel and animal safety including biohazardous
waste and carcass disposal and cage decontamination in the following chart:
* Biosafety must be addressed in the protocol.
** Radiation safety must be addressed in the protocol and radiation licensure is required.
Licensure Number: Click here to enter text.
*** Legend Drugs and Controlled Substances:
If you use Legend substances, do you have the appropriate licenses and legend and approval for the drugs you
will use in your project or class? ☐ YES ☐ NO
In Tennessee, it is not legal to divert drugs from clinical use to teaching and research use. You, or someone in
your group acting as an agent for the group, must have a license for each individual legend drug that you might
use in teaching or research. A legend drug is one where the label says “Federal Law restricts this drug to use by
or on the order of a licensed veterinarian” or “Federal Law prohibits dispensing without a prescription”. If the
drug is a controlled substance, you also need a license from the Drug Enforcement Administration for the
specific drugs that you may use. Obtaining and using such drugs for research will be reviewed.
Agent Concentration Route of
Administration
Duration of
Exposure
Post Exposure
Maintenance of
Animals
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I. USE OF RECOMBINANT MATERIALS
Recombinant nucleic acid molecules are those that are constructed outside living cells by joining natural or synthetic nucleic
acid segments to nucleic acid molecules that can replicate in a living cell, or molecules that result from the replication of
such. This includes generation and/or use of genetically-modified microorganisms, viruses, or cell lines; transgenic plants
or animals; and/or recombinant vaccines or therapeutic agents that are NOT licensed (commercially available) or otherwise
deregulated by a U.S. Federal authority (EPA, FDA, or USDA).
The use of recombinant DNA molecules in conjunction with animals must be declared by the PI in their IACUC protocol
for Biosafety evaluation and risk assessment. In most instances, the use of recombinant or synthetic nucleic acid molecules
in conjunction with animals will require approval by the Institutional Biosafety Committee (IBC) in order to ensure
compliance with the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules.
Please select the following recombinant materials to be used in this protocol and describe below:
☐ Purified recombinant/synthetic nucleic acid molecules
☐ Genetically-modified microorganisms (archaea, bacteria, fungi, protozoa)
☐ Genetically-modified viruses/viral vectors
☐ Genetically-modified cell lines (e.g. human cell xenografts)
☐ Purified recombinant products (e.g. proteins, hormones, etc.)
☐ Recombinant vaccines or other therapeutic agents
☐ Transgenic plants
☐ Transgenic animals that are created and/or used at UTC:
a. Describe approach or procedure to produce modification:
Click here to enter text.
b. What gene(s) were intentionally modified:
Click here to enter text.
c. If known, expected product over expressed / under expressed:
Click here to enter text.
d. Describe any potentially debilitating phenotypes associated with this animal:
Click here to enter text.
Briefly summarize the proposed recombinant materials:
Click here to enter text.
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J. SUBSTANCE(S) COLLECTED FROM LIVE ANIMALS
Substance Collected*
Site of collection Method of
collection
Amount Collected Frequency of Collection(s)
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K. OTHER PROCEDURES NOT DESCRIBED ABOVE
Please describe in detail what will be done to animals (this response should provide the IACUC with a clear
understanding of procedure(s) to be performed on an animal or group of animals not covered in other sections.
1. Describe animal methodology/manipulation/test(s) to be performed:
Click here to enter text.
2. Duration:
Click here to enter text.
3. Frequency of tests:
Click here to enter text.
4. Potential adverse events:
Click here to enter text.