Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Faculty of Veterinary Medicine
Gadjah Mada University
Workshop / Training on
Biotechnology Platform for Research and Training on Parasitic Zoonoses in South East Asia
Yogyakarta, May 19 – 24, 2014
with the financial support of the French Ministry of Foreign Affairs
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Trypanosomes and their vectors
• Trypanosoma evansi causes a trypanosomosis (Surra), mechanically transmitted by haematophagous flies, such as Tabanids and Stomoxes.
• The principal host species of T. evansi varies geographically in Indonesia, horses, buffalos and cattle are particularly affected, including wildlife and rodents.
• Human infections have been recently described in India, surra a potential emerging parasitic zoonosis.
• Rodents are also a reservoir of Trypanosoma lewisi, that could be found in Indonesia
Trypanosomiasis in Indonesia
Cattle Trypanosomiasis
Buffalo Trypanosomiasis Cattle and Buffalo Trypanosomiasis
Horse Trypanosomiasis (Cattle also)
Sumatra
Malaysia
West Papua
East Nusa Tenggara
West Nusa Tenggara
Sulawesi
Kalimantan
Yogyakarta
Singapore
Thailand
Malaysia
Philipines
Australia
Java
BioTraps
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Vectors and Pathogens Suborder Family Species
Nematocera Culicidae Anopheles sp (malaria vector)
Culex sp (virus vector: Dengue, Arbovirus, etc)
Simuliidae Simulium sp (Onchocercosis)
Ceratopogonidae Culicoides sp (Leucocytozoonosis, Blue tongue)
Psychodidae Phlebotomus sp (Leishmaniasis)
Brachycera Glossinidae Glossina sp (Trypanosomiasis)
Tabanidae Muscidae
Tabanus sp (Trypanosomiasis, Anaplasmosis) Musca sp (bacterial vector) Stomoxys sp (Trypanosomiasis, Dermatophilus)
Family: Muscidae (House-flies and Stable-flies)
• Musca domestica
• Mechanical transmission of pathogens, accidental myiasis.
• Stomoxys calcitrans
• biting pest (human and veterinary pest)
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Trypanosoma and Surra in Indonesia
• Many Trypanosomosis outbreaks occur in bovines in Indonesia, and many biting flies as mechanical vector of Trypanosomosis.
• 28 species of Tabanus, 5 Chrysops species and 5 Haematopota sp. were reported as potential vectors of surra in Indonesia
• The high density of stable fly populations can severely decrease livestock productivity.
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Outbreak in Sumba Island
• Tabanus and Stomoxys flies has a significant impact in horses and cattle trypanosomosis in Sumba Island in East Nusa Tenggara province of Indonesia during a Surra outbreak in 2012-2013 (393 cases, 350 dead).
• In several Islands in Indonesia, Tabanus rubidus can also transmit zoonotic disease such as Antrax. To date, there is no control method available.
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Trypanosomosis in Horses
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Good farming system
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Garbage farming system
Traditional farming system
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Traditional farming system
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Dairy cattle livestock
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
NZ1 Fly Trap
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Fogging
Sticky paper
Herbal fogging Insecticide
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Aims of the workshop
• To share knowledge and experience on trypanosomes and their vectors: diagnosis epidemiology, impact and control of the disease as well as trapping, identification, studies of transmission mechanisms, epidemiological impact as vectors and ways of control.
• To strengthen animal health systems approach, focusing on national policy, coordinated at different levels of government to increase technical skills.
• To support the sustainable Indonesia’s veterinary services in Surra erradication according to international standards set by the World Organization for Animal Health (OIE).
• To develope joint research projects amongst French and South East Asian research institutes and universities.
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
“One Health Open workshop” 19th May 2014
• The workshop will be held under the umbrellas of GREASE and the NAHIAT, in order to strengthen cross-sectorial and multidisciplinary exchanges about trypanosomosis management among professional form different sectors (Animal health, human health, environment, rural development).
• Lectures and open discussions will be held on future collaborations in the One Health (OH) conceptual framework, on trypanosomes and vectors, including livestock, wildlife, environment (both social and natural), veterinary and human medicine
• it will be open to a large audience, especially to represent various geographical areas of Indonesia.
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
“Registered Workshop” 20th to 24th May 2014
• Limited to 20 participants
• Presentations:
(1) trypanosomes both in humans and animal reservoir in Asia, diagnosis methods and potential control methods, and
(2) their transmission mechanisms and vectors, including trapping, identification and control.
• It will include lectures, field work, practices and laboratory activities
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Expected results and outputs
• To overview the problem of trypanosomes in Asia, both in humans and animals including livestock, domestic and wild animal;
• To implement and interpret specific diagnosis of trypanosomoses in mammals, using parasitological, serological and DNA based methods;
• To implement trapping and identification of the main genera/species of biting insects acting as mechanical vectors of trypanosomes in livestock;
• To draft common research project for the control of biting insects within a OH framework
• To build up research methodology for epidemio-surveillance of trypanosomoses.
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Time Programme Lecture/Speaker duration
International seminar on Biting Flies and Trypanosomes
09.00 – 09.05 Opening remarks from the committee
Dr. Wisnu Nurcahyo (GMU) 5 min T
09.05 – 09.15 Opening Speech from GMU Prof. Dr. Suratman (Vice Rector Research GMU)
10 min T
09.15 – 09.25 Opening Speech from coordinator of BioZoonoSEA
Prof. Sathaporn Jittapalapong (KU)
10 min T
09.25 – 09.30 Opening Speech from NAHIAT coordinator (CIRAD-IRD)
Dr. Marc Desquesnes (CIRAD / KU)
5 min T
09.30 – 09.45 Coffee break 15 min
Morning Session
09.45 – 10.35 L 1 : One Heath / EcoHealth conceptual framework
Dr. A Binot 50 min T
10.35 – 10.55 L 2 : Parasitic Zoonoses in Thailand Pr. S Jittapalapong 20 min T
10.55 – 11.15 L 3 : VRI and zoonoses in Malaysia Dr C Panchadcharam 20 min T
11.15 – 11.35 L 4 : Atypical Human Trypanosomoses in SEA
Dr S Herder 20 min T
11.35 – 11.55 L 5 : Biting-Insects as mechanical vectors
Dr M Desquesnes 20 min T
11.55 – 12.15 L 6 : Parasitic zoonoses in Indonesia
Dr Wisnu Nurcahyo 20 min T
12.15 – 13.15 Lunch Time (Courtesy of FVM GMU) 60 min
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
13.15 – 13.30 L 7 : Surra in the Philippines Dr A Dargantes 15 min T
13.30 – 13.45 L 8 : Surra in Malaysia Dr C Panchadcharam 15 min T
13.45 – 14.00 L 9 : Surra in Thailand Pr S Jittapalapong 15 min T
14.00 – 14.15 L 10: Surra in Indonesia Dr April Wardana (Indonesian Veterinary Research Institute)
15 min T
14.15 – 14.30 L 11 : Molecular aspects of surra Pr W Artama 15 min T
14.30 – 14.45 L 12: Economic impact of surra Dr A Dargantes 15 min T
Regional aspects of Surra in Indonesia (90 min) 14.45 – 14.55 L 13 : Surra in Bali Dr. Masa Tenaya
(Bali Diseases Investigation Center) 10 min T
14.55 – 15.05 L 14 : Surra in Java Dr. Fajar Sumping Catur Rasa (Yogyakarta Diseases Investigation Center)
10 min T
15.05 – 15.15 L 15 : Surra in Sumatra Syamsul Maarif, DVM, MSc (Lampung Diseases investigation Center)
10 min T
15.15 – 15.25 L 16 : Surra in Kalimantan Sulaxono Hadi, DVM (Banjarbaru Diseases Investigation center)
10 min T
15.25 – 15.35 L 17 : Surra in Sulawesi Bagoes Purma Jaya, DVM, MSc (Makassar Diseases Investigation Center)
10 min T
15.35– 15.45 L 18 : Surra in Sumba Martono Adi Priyatno, DVM (Veterinarian in East Sumba District)
10 min T
15.45 – 16.00 Coffee break 15 min
16.00 – 16.15 L 19 : Surra in Regulation of surra in indonesia
Dr. Pujiatmoko (Director of Animal Health)
15 min T
16.15 – 16.25 L 20: GREASE network Dr A Binot 10 min T
16.25 – 16.50 Open Discussion on One Health Concepts : Biting flies and Trypanosomes
Dr A Binot (CIRAD / GREASE) 25 min T
16.50 – 17.00 Closing remarks Dr. A Binot 10 min T
17.00 – 17.05 Announcement Dr. Wisnu Nurcahyo 5 min T
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Day 2 : Workshop/Training - Tuesday, May 20th, 2014: Biting flies biology & trapping
Time Programme Lecture/Speaker Place/Time Remark
08.30 – 09.00 30 min
Lecture 1 : An overview on biting insects
Dr Marc Desquesnes T
09.00 – 10.45 105 min
Lecture 2 : Biology of Tabanids and stomoxyine flies
Dr Marc Desquesnes T
10.45 – 11.00 Coffee break 15 min
11.00 – 11.30 30 min
Lecture 4 : Seasonality of tabanids in Thailand
Dr Jumnongjit Phasuk T
11.30 – 12.00 30 min
Lecture 5 : Seasonality of stomoxes in Thailand
Dr Jumnongjit Phasuk T
12.00 – 13.00 Lunch Time 60 min
13.00 – 14.15 75 min
Lecture 6 : Trapping technologies
Dr Marc Desquesnes T
14.15 – 14.30 Coffee break 15 min
14.30 – 17.30 3 hours
Field trip 1: Setting up of insect traps in the Field (KP4 – GMU)
Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Wisnu Nurcahyo Dr Alan Dargantes
P
17.30 – 18.00 Back to UC Hotel
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Day 3: Workshop/Training - Wednesday, May 21th, 2014: Biting flies trapping & identification
Time Programme Lecture/Speaker Place/Time Remark
08.00 – 10.00 2 hours
Field trip 2: Collect of cages from traps in the field KP4 – UGM
KP4 GMU P
10.00 – 10.15 Coffee break 15 min
10.15 – 12.00 105 min
Lecture 7: Identification of biting flies: generalities & Tabanids
Dr Marc Desquesnes FVM GMU P
12.00 – 13.00 Lunch Time 60 min FVM GMU
13.00 – 14.45 105 min
Lecture 8: Identification of Stomoxys, Hematobia and Musca crassirostris
Dr Jumnongjit Phasuk FVM GMU T
14.45 – 15.00 Coffee break 15 min FVM GMU
15.00 – 17.00 2 hours
PW1: Identification of biting insects collected in the morning
Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes
FVM GMU P
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Day 4: Workshop/Training - Thursday, May 22th, 2014: Biting flies trapping, identification & control;
Trypanosomes: biology & identification
Time Programme Lecture/Speaker Place Remarks
08.00 – 10.00 Field trip 3: Setting up of insect traps in the field of dairy cattle Kaliurang, Sleman
Kaliurang P
10.00 – 10.45 Lecture 9 : Control of biting flies
Dr Marc Desquesnes T
10.45 – 11.00 Coffee break 15 min
11.00 – 12.00 Lecture 10 : Biology, morphology and pathogeny of Trypanosomes
Dr Stéphane Herder T
12.00 – 13.00 Lunch Time (lunch box) 60 min FVM GMU
13.00 – 13.30 Lecture 11 : Parasitological diagnosis of Trypanosomes
Dr Marc Desquesnes T
13.30 – 14.00 Lecture 12 : Serological diagnosis of Trypanosomes
Dr Marc Desquesnes T
14.00 – 14.15 Coffee break 15 min FVM GMU
14.15 – 17.00 PW2: Identification of biting insects collected in the morning
Dr Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes
FVM GMU P
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Day 5 : Workshop/Training - Friday, May 23th, 2014: Biting flies trapping & identification Trypanosomes: Molecular
diagnosis, characterization and control
Time Programme Lecture/Speaker Place Remarks
08.00 – 10.00 Field trip 4: Setting up of insect traps in the field of beef cattle Glagah Beach, Bantul
Kaliurang P
10.00 – 10.45 Lecture 13: Molecular diagnosis of Trypanosomes
Dr. Wayan Artama FVM GMU
10.45 – 11.00 Coffee break 15 min
11.00 – 12.00 Lecture 14 : Molecular characterization of Trypanosomes
Dr. Stéphane Herder FVM GMU
12.00 – 13.00 Lunch Time 60 min FVM GMU
13.00 – 13.30 Lecture 15: Control of surra in the Philippines
Dr. A Dargantes FVM GMU P
13.30 – 14.00 Lecture 16: Control of surra in Thailand
Dr. Marc Desquesnes FVM GMU P
14.00 – 16.00 PW3: Identification of biting flies from dairy cattle Kaliurang, Sleman
Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes
FVM GMU P
16.00 – 16.15 Coffee break 15 min FVM GMU
16.15 – 17.15 PW4: Practical work in the laboratory for parasitological diagnosis of trypanosomes
Dr. Marc Desquesnes Dr Stéphane Herder Dr Wayan Artama Dr Wisnu Nurcahyo
T
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Day 6 : Workshop/Training - Saturday, May 23th, 2014: Biting flies identification Trypanosome molecular diagnosis
Time Programme Lecture/Speaker Place Remarks
08.00 – 12.00 Identification of biting flies from beef cattle Glagah Beach, Bantul
Dr. Marc Desquesnes Dr Jumnongjit Phasuk Dr Alan Dargantes
FVM GMU P
12.00 – 13.00 Lunch Time 60 min FVM GMU
13.00 – 16.00 PW5: Practical work in the laboratory of Molecular biology for Trypanosomes
Dr Stéphane Herder and Prof. Wayan Artama
FVM GMU P
16.00 – 17.00 Conclusions and Closing ceremony
Dr. Marc Desquesnes Dr. Joko Prastowo
CIRAD/IRD Dean FVM
GMU
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
No Name of Institution Origin (Province)
1 Faculty of Veterinary Medicine, UGM Yogyakarta
2 Faculty of Veterinary Medicine, University of Udayana Bali
3 Faculty of Veterinary Medicine, Airlangga University, Surabaya East Java
4 Faculty of Veterinary Medicine, Brawijaya University, Malang East Java
5 Faculty of Veterinary Medicine, Nusa Cendana, Kupang East Nusa Tenggara
6 Faculty of Veterinary Medicine, Syiah Kuala Univ, Banda Aceh Banda Aceh
7 Faculty of Veterinary Medicine, NTB Univ Mataram West Nusa Tenggara
8 Faculty of Veterinary Medicine, Wijayakusuma, Surabaya East Java
9 Veterinary Study Programme, Hassanuddin Univ, Makassar South Sulawesi
10 Bogor Agriculture University, Bogor West Java
11 Veterinary Research Institute, Bogor West Java
12 Disease Investigation Center, Yogyakarta Yogyakarta
13 Disease Investigation Center, Denpasar, Bali Bali
14 Disease Investigation Center, Banjarbaru, Kalimantan Kalimantan
15 Disease Investigation Center, Lampung South Sumatra
16 Disease Investigation Center, Bukittinggi, West Sumatra West Sumatra
17 Disease Investigation Center, Medan North Sumatra
18 Disease Investigation Center, Makassar South Sulawesi
19 Faculty of Biology, UGM Yogyakarta
20 Faculty of Medical, UGM Yogyakarta
21 Vector Research Station, Ministry of Health, Salatiga Central Java
22 Rodents Research Station, Banjarnegara Central Java
23 Zoonoses Division, Ministry of Health, Jakarta Jakarta
24 Directorate of Animal Health, Ministry of Agriculture Jakarta
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Activities
International Seminar
• Keynote Speakers (CIRAD, GREASE)
• Invited speakers (Asean Country)
Theory and Practical workshop
• Biting flies
• Trypanosomes
Field trips
• Bionomics and habitats of vectors
• Trapping and Field identification
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Secretariat: Dept of Parasitology Faculty of Veterinary Medicine Gadjah Mada University Jalan Fauna 2, Karangmalang Yogyakarta 55281 Email: [email protected] [email protected] Website : www.fkh.ugm.ac.id Phone and fax : +62-274-6411525 Mobile : +62-813 25252 7887
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Workshop Thailand
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Workshop at NUS Singapore
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
Workshop @ VRI Malaysia
Diagnosis and control of human and animal trypanosomes and their vectors in Asia: from field to laboratory
3/28/2014 Institute for Research and Community
Services, UGM 34
City tour Yogyakarta
Thank you for your attention
See you in Indonesia May 19-25, 2014