Post on 18-Jan-2018
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© 2015 BioLife Solutions, Inc.© 2015 BioLife Solutions, Inc.
HypoThermosol®
Performance Evidence
© 2015 BioLife Solutions, Inc.
5-Day Hypothermic Storage of Human DermalFibroblasts Enabled by HypoThermosol®
alamarBlue® stain for metabolic activity
* Contains serum
Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5
© 2015 BioLife Solutions, Inc. 4
Representative Fluorescent Micrographs Illustrating Morphology of Normal Human Dermal Fibroblasts
Stored in HypoThermosol®
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst 40X magnification
Media with Serum
3 Day Storage
5 Day Storage
Celsior®
3 Day Storage
5 Day Storage
Viaspan®
3 Day Storage
5 Day Storage
HypoThermosol®
3 Day Storage
5 Day Storage
© 2015 BioLife Solutions, Inc. 6alamarBlue® stain for metabolic activity
* Contains serum
Extended Hypothermic Storage of Human MesenchymalStem Cells Enabled by HypoThermosol®
Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5 DAY STORAGE7
© 2015 BioLife Solutions, Inc. 7
Representative Fluorescent Micrographs Illustrating Morphology of Human Mesenchymal Stem Cells Stored in HypoThermosol®
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst 20X magnification
Normosol®-R
3 Day Storage
5 Day Storage
Plasma®-Lyte
3 Day Storage
5 Day Storage
Viaspan®
3 Day Storage
5 Day Storage
HypoThermosol®
3 Day Storage
5 Day Storage
© 2015 BioLife Solutions, Inc. 9
Recovery of Human Osteoblast Cells Following Hypothermic Storage Assayed 24 Hours Post Storage
Differentiated Following 5-Day Hypothermic Storage of Undifferentiated hMSC in HypoThermosol® Assayed 24 Hours Post Storage - % Recovery of Viable Cells
DAY STORAGE1 DAY STORAGE3 DAY STORAGE6
alamarBlue® stain for metabolic activity
* Contains serum
© 2015 BioLife Solutions, Inc. 10
Representative Fluorescent Micrographs Illustrating Morphology of Human Osteoblast Cells Following Hypothermic Storage
20X magnification
Differentiated Following 5 Day Hypothermic Storage ofUndifferentiated hMSC in HypoThermosol
Green Actin CytoskeletonPhalloidin (FITC)
Red Mitochondria ActivationMitoTracker® Red
Blue Nuclear StainHoechst
Normosol®-R
3 Day Storage
6 Day Storage
Plasma®-Lyte
3 Day Storage
6 Day Storage
Viaspan®
3 Day Storage
6 Day Storage
HypoThermosol®
3 Day Storage
6 Day Storage
© 2015 BioLife Solutions, Inc. 12
24 Hour Post Storage Recovery of Human Dental PulpStem Cells Following Hypothermic Storage
Commercial Solution Comparison: Serum and Serum-Free Media
DAY STORAGE1 DAY STORAGE3 DAY STORAGE5
% Recovery of Viable Cells
alamarBlue® stain for metabolic activity
* Contains serum