Post on 27-Sep-2020
transcript
Performance of microbial induced carbonate precipitation for immobilizing Cd
in water and soil
Dinghua Peng1, Bin Wu1, Suyu Qiao1, Hang Ma1, Lei Zhang1, Siyu Hou1, Bin Wu1
Heng Xu1*
1Key Laboratory of Bio-Resource and Eco-Evironment of Ministry of Education,
College of Life Sciences, Sichuan University, Chengdu 610065, P.R.China
Table captions for supplementary material
11* Corresponding author: xuheng64@sina.com (H.Xu). Tel: +86 28 85414644; Fax: +86 28 85418262
Table S1. Main properties of soil, OS and urea used in the experiment
Table S2. Urease activity between Cd-resistant bacteria strains
Table S3. Effect of different treatments on soil bacterial community richness and
diversity indices at 40 days after incubation
Figures captions for supplementary material
Figure S1. Color change of solid urease selective medium with un-inoculation (a) and
inoculation (b) Cd-resistant bacteria cell
Figure S2. The growth rate of bacterial cells
Figure S3. Images of precipitation induced by strain CJW-1 with 40 mM CaCl2 and
2% urea in 40 mg/L Cd contaminated medium.
Table S1 Main properties of soil, OS and urea used in the experimentProperty Soil OS Urea
Sand (%) 26.23 - -Silt (%) 22.35 - -Clay (%) 51.42 - -
pH 7.20 8.52 9.24Organic matter(g/kg) 41.00±0.53 12.60±0.22 -
Cation exchange capacitya (cmol/kg)
19.80±0.33 1.32±0.12 -
Total N (g/kg) 2.28±0.29 1.46±0.09 - Total P (mg/kg) 693±0.22 601±1.12 -Total K (g/kg) 17.70±0.31 2.00±0.30 -
Total Cd (mg/kg) 20.62±0.13 0.12±0.02 -Values are expressed as mean ± standard error (n=3).
Table S2 Urease activity between Cd-resistant bacteria strains
Isolated strain Urease activity
CJW-1 3.242CJW-2 0.021
Urease activity was represented by OD values in solution at day 5
Table S3 Effect of different treatments on soil bacterial community richness and
diversity indices at 40 days after incubation
Treated samples Ace a Chao 1a Shannon b Simpson b Coverage OTUs
CK 1492.79 1529.10 5.26 0.018 99.29% 1268
T1 1281.35 1280.10 4.83 0.021 99.20% 1003
T2 1463.18 1478.95 5.15 0.020 99.35% 1279
T3 1332.73 1309.26 4.58 0.052 99.27% 1116
T4 1379.96 1424.10 4.54 0.050 99.13% 1119
T5 906.81 862.22 1.65 0.599 99.21% 646
T6 1169.47 1167.17 3.59 0.159 99.15% 869
T7 1146.62 1174.41 4.15 0.078 99.28% 894
a Richness indices b diversity indices
Figure S1 Color change of solid urease selective medium with un-inoculation (a) and
inoculation (b) Cd-resistant bacteria cell
a
b
Figure S2 The growth rate of bacterial cells
Figure S3 Images of precipitation induced by strain CJW-1 with 40 mM CaCl2 and
Un-inoculation with Cd-resistant bacteria
Inoculation with strain CJW-2Inoculation with strain CJW-1
2% urea in 40 mg/L Cd contaminated medium.