Post on 11-Jan-2016
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Bell Ringer 10/22/13Bell Ringer 10/22/13Respond to the following in your Respond to the following in your notes:notes:
1.1.Draw a nucleotide and label the Draw a nucleotide and label the parts (don’t cheat – see if you parts (don’t cheat – see if you remember!)remember!)
2.2.What is the purpose of PCR? (this What is the purpose of PCR? (this was in your Ch. 9 HW)was in your Ch. 9 HW)Leave the sheets on your desk alone for now – we will Leave the sheets on your desk alone for now – we will look at those in a bitlook at those in a bit
DNA ReplicationDNA Replication
DNA ReplicationDNA Replication
Replication = DNA copies itself Replication = DNA copies itself exactlyexactly(Occurs within the nucleus)(Occurs within the nucleus)
Any mistake in copying = Any mistake in copying = mutationmutation
A. A. Basic Facts of DNA Basic Facts of DNA ReplicationReplication
1. 1. Complementary Complementary base pairing base pairing makes makes replication possiblereplication possible
C C - - GGA A - - TT
A. A. Basic Facts of DNA Basic Facts of DNA ReplicationReplication
2. One side of DNA 2. One side of DNA molecule is a molecule is a templatetemplate for making for making the other side the other side (strand)(strand)
B. Process of DNA ReplicationB. Process of DNA Replication
1.1. Uncoil & unzip DNA moleculeUncoil & unzip DNA molecule
Enzyme (-ase)Enzyme (-ase) breaks breaks
weakweak Hydrogen Bonds Hydrogen Bonds
between basesbetween bases
B. B. Process of DNA ReplicationProcess of DNA Replication
2. 2. Enzyme brings in complementary N-basesEnzyme brings in complementary N-bases
DNA Polymerase
B. B. Process of DNA ReplicationProcess of DNA Replication
3. New hydrogen bonds form 3. New hydrogen bonds form between nitrogenous bases between nitrogenous bases of the template strand and of the template strand and the newly-forming daughter the newly-forming daughter strand. strand.
DNA Replication TutorialDNA Replication Tutorialhttp://highered.mcgraw-hill.com/sites/9834092339/student_view0/chapter14/dna_replication.html
C. Semi-conservative replication
DNA vs. RNA - StructureDNA vs. RNA - Structure
DNADNA RNARNA
Sugar = Sugar = deoxyribosedeoxyribose Sugar = Sugar = riboseribose
DoubleDouble-stranded -stranded moleculemolecule
SingleSingle-stranded -stranded moleculemolecule
ThymineThymine bonds with bonds with adenineadenine
UracilUracil instead of thymine instead of thymine
DNA vs. RNA – Add’tl infoDNA vs. RNA – Add’tl info
DNADNA RNARNA
Nuclear Nuclear DNADNA
MitochondrialMitochondrial DNA DNA
ChloroplastChloroplast DNA DNA
mRNAmRNA = messenger = messenger
tRNA tRNA = transfer= transfer
rRNArRNA = ribosomal = ribosomal
Nuclear DNA Nuclear DNA never never leaves the nucleusleaves the nucleus
Assembled in nucleus, Assembled in nucleus, moves to cytoplasmmoves to cytoplasm
((leaves the nucleusleaves the nucleus))
RNA RNA vs. vs. DNADNA