Collect Environmental Sample

Collect Lysate from Plate with Highest Plaque Density

Spot Titer

Full Plate Titer

Prepare Webbed Plates

Plaque Assay

Pick a Plaque & Resuspend

Serially Dilute Sample with Phage

Extract Phage with SMALL

Volume of Media

Spin & Filter to Remove

Particulate & Bacteria

Test for Phage by Plaque Assay

Extract Phage with LARGE

Volume of Media

Spin & Filter to Remove

Particulate & Bacteria

Add Host Bacteria& Incubate

Spin to Remove Host Bacteria

Test for Phage by Spot Test

Collect Lysate from Webbed Plates

Spot Titer

Full Plate Titer

?Sample Contains Phage?

? Plaque Morphologies Consistent?

? Titer > 10 pfu/ml?9

ISOLATION PURIFICATION

Image by Electron Microscopy

MICROSCOPY

AMPLIFICATION

Compare Phages by Restriction Digest of DNA & Gel Electrophoresis

Extract Phage DNA

EXTRACTION

CHARACTERIZATION

Archive & Create phagesDB Entry