Post on 18-Jan-2016
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BIOLOGY 12
Enzymes
Enzymes
Video
Enzyme BasicsEnzyme Basics
Like all catalysts, enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction. As a result, products are formed faster and reactions reach their equilibrium state more rapidly. Most enzyme reaction rates are millions of times faster than those of comparable un-catalyzed reactions. As with all catalysts, enzymes are not consumed by the reactions they catalyze, nor do they alter the equilibrium of these reactions.
Enzymes
proteins catalysts for biological reactions substrate specific they initiate chemical reactions without being
used in the reaction (reusable) usually named after their substrate, most end in
“ase” (ex. Maltase) “Lock and Key” Fit Induced Fit Model
Enzymes
they lower the activation energy, therefore increase the rate of reaction
Enzymes
activity is lost if they are denatured the unfolding and inactivation – by heating or
chemical denaturants, which disrupt the three-dimensional structure of the protein
Enzymes
How Enzymes Work
Enzyme Action
Lock and Key Model substrate binds to active site of enzyme enzyme-substrate complex forms substrate reacts and product is formed product is released (enzyme is unchanged by
reaction)
Enzyme Action
Enzyme Action
Induced Fit Model scientists have modified the “lock and key”
model enzyme structure is flexible – not rigid the shape of the enzyme’s active site and the
shape of the substrate do not need to match exactly
Enzyme Action
when the enzyme and substrate join, the enzyme changes its shape slightly (adjusts to fit)
this increases the range of substrate specificity
Enzyme Action
Catalytic Cycle of an Enzyme
Activation Energy (EA)
the minimum amount of energy required to start a chemical reaction
examples: Oxygen gas + Hydrogen gas + HeatHeat (A.E) =
Water Wood + Oxygen + Burning MatchBurning Match (A.E) =
Burning wood
Activation Energy (EA)
enzymes use a variety of mechanisms that lower activation energy and speed up a reaction
*see graphs on page 69 Activation Energy Graph
example of why we have enzymes: enzymes allow reactions to take place at lower
temperature (i.e. body temperature) the reactions of living organisms cannot depend
on heat as a source of activation energy high temperatures would destroy cells heat would be far too random of a source of
activation energy (not dependable) cells must carry out specific reactions at definite
times
Recap on Enzymes
each is specific – guides only one type of cell reaction
they are not permanently changed or used up during a reaction
can be used over and over again the shape of the enzyme is crucial to the
reaction
Factors Affecting Enzyme Activity
environmental factors
cofactors and coenzymes
enzyme inhibitors
Factors Affecting Enzyme Activity
temperature up to a point, the speed of the reaction
increases with the increase in temperature most enzymes function optimally around body
temperature (between 35o – 40oC) extreme temperatures can denature an enzyme
Factors Affecting Enzyme Activity
pH the optimal pH is between 6 and 8 (near
neutral)
Factors Affecting Enzyme Activity
cofactors inorganic non-protein helpers are sometimes
needed for optimal enzyme activity examples:
vitamins zinc iron must be present in the quaternary structure
of hemoglobin in order for it to pick up oxygen
Factors Affecting Enzyme Activity
enzyme inhibitors competitive:
has a structure similar to the substrate competes with the substrate for the active site occupies the active site
Factors Affecting Enzyme Activity
enzyme inhibitors non-competitive:
does not have a structure like the substrate binds to the enzyme but not active site changes the shape of enzyme and active site substrate cannot fit into altered active site no reaction occurs
Factors Affecting Enzyme Activity
Factors Affecting Enzyme Activity
How Enzymes Work
Lets Label It
Questions
1. You turn on a battery powered tape deck and the reels in the cassette begin to rotate.
How does this example explain energy transformation?
A. Potential energy (stored energy) in the batteries was converted to kinetic energy (mechanical energy).
Questions
2. Suppose you started a fire by using a magnifying glass to focus the sun’s image on some paper.What is the source of activation energy for this reaction?
A. The concentrated sunlight was absorbed and changed to heat. This heat provided the activation energy.
Questions
3. Explain how the enzyme maltase can control a reaction involving maltose, but not one involving sucrose.
A. Maltose consists of two bonded glucose units, whereas sucrose consists of a glucose bonded to a fructose.
If the active site of the enzyme maltase fits with maltose, it will not fit with sucrose.
Compare and Contrast
Kinetic Energy / Potential Energy Endergonic / Exergonic Enzyme/ Substrate Lock and Key Model / Induced Fit Model Enzyme / Coenzyme Competitive Inhibitors / Non-Competitive
Inhibitors
Questions
Page 77 #1, 4, 5, 6, 7, and 8.