CHANGE IN ACID ERITHROGRAM

OF LABORATORY ANIMALS IN

CONSTANT ELECTRIC AND

MAGNETIC FIELD

Anna V. Novikova, Anna A. Oleshkevich,

Viktor E. Novikov

Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

(Moscow SAVMB), Moscow, Russia

kaffizmgavmib@mail.ru

Section: Biophysics, Medical Physics

SIX INTERNATIONAL CONFERENCE ON RADIATION AND APPLICATIONS IN VARIOUS FIELDS OF RESEARCH

12/06/ - 16/06/ 2018 | Slovenska Plaza | Budva | Montenegro

RAD 2018

AUTOHEMOTHERAPY

PENDULUM TYPE

EMR

effect of a constant electric and magnetic

field on the suspension of red blood cells

of laboratory rats

+

-

8.3102

V/м

70 Э (5.5 102А/м)

photoelectric turbidimeter

1- photometer- turbidi-meter; 2- cuvette`s thermostatic water-jacket; 3- cuvette with suspension of erythro-cytes; 4- mixer; 5-fitting for fluid supply; 6 - optical sensor block; 7 - analog-digital converter; 8 – recorder (PC+ software) ; 9 –light.

0

0,2

0,4

0,6

0,8

1

0 500 1000

time, s

ch

are

of

lys

is c

ells

ACIDERITHROGRAM (cumulative)ACIDERITHROGRAM (differential)

0

0,0005

0,001

0,0015

0,002

0,0025

0 200 400 600 800 1000

time, s

[ch

are

cells]/

[s]

• The resulting distribution of

erythrocytes by acid resistance

(histogram) was further analyzed by

mathematical modeling. It was

carried out by the histogram

decomposing into the sum of 7

normal distributionsseries. To each

private distribution corresponds one

age group of erythrocytes.

)](exp[7

1

)(32

21 jj

jji

KtKKj

j

Ktdt

dn

t – the time from preface hemolytic ;

(К1, К2, К3) – characteristics distribution .

К1 – partial coefficient one group erythrocytes (the parts of total volume);

К2 – conform to dispersion value;

К3 – the size of time attainment maximum speed hemolysis one group erythrocytes.

0

0,0002

0,0004

0,0006

0,0008

0,001

0 400 800

time, s

N -1

(d

n/d

t) K1=(SDn/N)

K3

K2

mathematical modeling by the histogram decomposing

into the sum of 7 normal distributionsseries

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0 200 400 600 800 1000

p1(t) p2(t) p3(t)

p4(t) p5(t) p6(t)

p7(t) SUM(t)

0

0,2

0,4

0,6

0,8

1

0 500 1000

experiment model

The simulation erithrogram one

Method reduce to automatic

selection coefficient`s signifi-

cance К1 – К3 (soft method ).

The coincidence simulation curve

and experimental

curve to hallmark adequacy

(to within width grass).

• The change of diffrential ACID ERITHROGRAM after coercion CONSTANT ELECTRIC FIELD

differential acid

erithrogram (control)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0 500 1000

time, s

[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram ( 5

min influence ESF)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0,0035

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram (10

min influence ESF)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0,0035

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram (15

min influence ESF)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0,0035

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

• The change of diffrential ACID ERITHROGRAM after coercion CONSTANT MAGNETIC FIELD

differential acid erithrogram

(control)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram (5

min influence CMFl)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram (10

min influence CMFl)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0,0035

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

differential acid erithrogram (15

min influence CMFl)

0

0,0005

0,001

0,0015

0,002

0,0025

0,003

0,0035

0,004

0,0045

0,005

0 500 1000

time, s[pa

rt o

f ly

sis

RB

C /

с]

• The change of integral (experimental curve normalized to unit) ACID ERITHROGRAM after coercion CONSTANT MAGNETIC FIELD

integral acid erirhrogram

(control)

0

0,2

0,4

0,6

0,8

1

0 500 1000

time, s

[pa

rt l

ysi

s R

BC

]

integral acid erirhrogram (5 min

influence CMF)

0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0,8

0,9

1

0 200 400 600 800 1000

time, s

[pa

rt l

ysi

s R

BC

]

integral acid erirhrogram (10 min

influence CMF)

0

0,2

0,4

0,6

0,8

1

0 200 400 600 800 1000

time, s

[pa

rt

lysi

s R

BC

]

integral acid erirhrogram (15 min

influence CMF)

0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0,8

0,9

1

0 200 400 600 800 1000

time, s

[pa

rt

lysi

s R

BC

]