Flag IP

Spt7-ve

IgG purified

Spt8Gcn5 Ada2 Ubp8 Taf5 4741250

SA

Spt3

1501 2

F3

83 4 5 6 97

SL

SASLF3160

1 2 3 4

Anti-myc AbA B

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31Fraction

SAGASLIKForm 3

670 kDavoid 158 kDa

Den

som

etry

C

SASLF3

TAP-Flag Spt7- + - +250-

150-

spt70 SPT7

1 2

D