Post on 14-Jul-2015
transcript
Shalini Adiga,Shivkumar Reddy, Ashok.M Department of Pharmacology
Kasturba Medical College,
Manipal University, Manipal.India
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IntroductionDiarrhoea is increased fluidity, volume or
frequency of bowel movementsAccording to WHO, it is the one of the most
common cause of morbidity and mortality in developing countries
Taxus baccata has been used used in the traditional medicine for asthma, bronchitis, rheumatism, high fever, diarrhea , indigestion, and epilepsy.
This herb has been scientifically evaluated for antidiarrheal activity .
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Objective
To investigate the anti-diarrhoeal activity of the T.baccata powderd leaves alcoholic extract
in castor oil-induced diarrhoeaBy charcoal meal test
Methodology The study was approved by IAEC, KMC, Manipal Animals: Male inbred albino rats of Wistar strain, weighing 200g- 250g fasted for 24 hrs were used. Two sets of 4 gps of rats (6 in no) was treated with respective drug orally.
Sl.No GROUPS
1 Normal Control –water
2 Loperamide -3mg/kg
3 Extract – 100mg/kg
4 Extract – 200mg/kg
Experimental diarrhoea
After 30 min 1 ml of castor oil was administerd orally to all the groups
the frequency of defecation and weight of faecal matter was noted up to 4 h in the transparent cages with an absorbent paper sheet placed at the base
Intestinal fluid - Rats anesthetised 60 min later by inhalation of ether. The intestine from pylorus to cecum dissected and contents measured
Effect of alcoholic extract of Taxus baccata on Castor Oil Induced Diarrhoea in Ratsgroups Mean Weight of
faeces after 4hrsPercent protection
control 30.00 ±0.57Loperamide -2mg/kg
12.75±0.30* 65.50%
Extract -100mg/kg 18.41± 3.21* 38.63%Extract -200mg/kg 15.33± 0.24* 48.90%
*P < 0.05 statistically (Mean±SEM) significant from control group (n=6)
Effect of alcoholic extract of Taxus baccata on castor oil induced Enteropooling in Rats
groups Mean volume of intestinal fluid (ml) ± SEM
% reduction
control 2.21 ± 0.11Loperamide -2mg/kg
0.85± 0.04* 75.56%
Extract -100mg/kg 1.73± 0.06 21.71%
Extract -200mg/kg 1.18± 0.05* 46.60 %
*P < 0.05 statistically (Mean±SEM) significant from control group (n=6
Charcoal meal test 24hrs fasted rats , treated with respective drugs
were administerd after 30 min orally 1 ml of 5% activated charcoal suspension . Rats anesthetised 30min later by inhalation of ether. Distance travelled by charcoal plug in the intestine measured and peristalitic index calculated.
Effect of taxus baccata leaf extract on gastrointestinal transitgroups peristalitic index
control 51.65± 0.96
Loperamide-2mg/kg 28. 75± 0.72*
Extract -100mg/kg 34.39± 1.65
Extract -200mg/kg 22.72± 0.62*
P < 0.05 statistically (Mean±SEM) significant from control group (n=6)
Discussion The Taxus buccata leaves extract inhibited the
castor oil induced diarrhea and reduced intestinal transit of charcoal
A dose dependent effect was seen and results were comparable to the standard drug loperamide.
The antisecretory and the antipropulsive effects could be sugested as the possible mechanism of the antidiarrheal activity
Conclusion
The study supports its use as antidiarrheal in herbal remedies.
Studies should be carried out for phytochemical evaluation of the active ingredients that promote antidiarrheal action.