Date post: | 15-Apr-2017 |
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VITAMINE E ROLE ON DUODENUM HISTOLOGICAL STRUCTURE OF WHITE RAT (Rattus norvegicus, Berkenhout,
1769) UNDER CADMIUM EXPOSURE
Shadrina Fildzah Ramadhani
Counselor: Drs. Yohanes Sugiyanto, M.S.Faculty of Biology
Universitas Gadjah Mada, Indonesia
Introduction
Vitamin E as antioxidant
Cadmium toxicity
Cadmium (Cd)
Cadmium effects inside
the body
• Ingested cadmium is poorly absorbed.
• Cadmium does not undergo metabolic conversion
• Given orally, cadmium chloride is absorbed quickly in the lower side of oral and digestive tract lamina mucosa
Tunica Submucosa
Tunica Serosa
Tunica Mucosa
Tunica Muscularis
(Kuehnel, 2003)
Figure 1. Histological structure of Duodenum
Duodenum
Vitamin E
• Vitamin E has been shown, in some studies, to inhibit lipid peroxidation, and forms an integral part of the cell membrane
• Vitamin E is able to protect biological membranes against free radical damage
Objective
The aims of the study is to determine the effect of cadmium on the duodenum histological structure of
white rat (Rattus norvegicus, Berkenhout, 1769) and the dose of vitamin E as antitoxic.
Methods
Instrumentation
Materials
• Mayer’s albumin• Xilol• Hematoksilin• Eosin• Canada Balsam
18 white rats (Rattus norvegicus Bekenhout,1769)
Given treatmentfor 4 weeks
Aquades 1 ml/day (K1)
CdCl2 1 ml at dose 1,5 mg/kg bw/day (K2)
Vitamin E 6,2 IU (K3)
Treatment
CdCl2 1 ml at dose 1,5 mg/kg bw/day + vitamin
E 3,15 IU (P1)CdCl2 1 ml at dose 1,5
mg/kg bw/day + vitamin E 6,2 IU (P2)
CdCl2 1 ml at dose 1,5 mg/kg bw/day +
vitamin E 12 IU (P3)Euthanasia,
NecropsyPreparations and
scoring
Control
Methods
Observation
Damage on Duodenum
• Degeneration of Lamina Propria
• Infiltration of Leukocytes • Dilated Gland• Villi Fusion• Edema
Scoring system
• 0 = normal, damage absent• 1 = low, damage present, not in all coupes, not
in all rats• 2 = moderate, damage present, in all coupes,
not in all rats; not in all coupes, in all rats• 3 = high, damage present, in all coupes, in all
rats
Results
Table 1. Scoring damage on Control Group
Damage K (1) 1 K (1) 2 K (1) 3 K (2) 1 K (2) 2 K (2) 3 K (3) 1 K (3) 2 K (3) 3
Degeneration of Lamina Propria 0 0 0 2 2 2 0 0 0
Infiltration of Leukocytes 0 0 0 1 1 2 0 0 0
Dilated Gland 0 0 1 2 1 1 1 0 0
Villi Fusion 1 0 0 2 2 2 0 0 0
Edema 0 0 0 1 1 0 0 0 0
Table 2. Scoring damage on Treatment Group
Damage P(1) 1 P (1) 2 P (1) 3 P (2) 1 P(2) 2 P(2) 3 P (3) 1 P(3) 2 P (3) 3
Degeneration of Lamina Propria 0 0 1 1 0 0 0 0 1
Infiltration of Leukocytes 1 0 0 0 0 0 0 0 0
Dilated Gland 1 0 1 0 0 1 1 1 0
Villi Fusion 1 1 1 1 0 0 0 1 0
Edema 0 1 0 0 0 0 0 0 0
Results
A B
Figure 2. Duodenum Histological Structure of Control Group (A) Aquades dan (B) Vitamin E (10x10)
Figure 3. Damages by Cadmium on Histological Structure of Duodenum (A) Degeneration of Lamina Propria (B) Infiltration of
Leukocytes (C) Dilated Gland (D) Villi Fusion (E) Edema(10x40)
A C
D E
B
A B
C
Figure 4. Histological Structure of Tteatment Group (A) Cadmium + vitamin E 3,15 IU (B) Cadmium + vitamin E
6,2 IU (C) Cadmium + vitamin E 12 IU (10x10)
Cadmium
• Causes inflamation on intestine mucose
• Uncontrolled poiferation of intestinum cells
• Stacking of fluid in the interstitial tissue
Vitamin E
• Vitamin E, are able to interact with oxidizing radicals directly.
• Vitamin E terminates the chain reaction of lipid peroxidation in bio-membranes and lipoproteins
Conclusion
Vitamin E repair the damage of histological structure on duodenum under cadmium expousre of white rats
The optimal dose of vitamin E that can repair the damage is 6,2 IU
Acknowledgement
Thank You