In The Name Of God

Precipitation

Antigen/Antibody ReactionPrimary reaction:

Immunoassay :Like Elisa , RIA , IFA

Secondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization ,

Precipitation : soluble antibody reacts with soluble antigen

Tertiary reaction : Detected by affect on tissues or cells.

Secondary Phenomenon

precipitation: proteinsSeveral types:Protein precipitationSRID DID

ImmunoElectherophoresis :Counter current IEP Rocket IEP

SRID plate preparation: (Agarose �+ BUFFER (Ab (Anti IgG ) Punch SamplingIncubation in RT for 24hmeasuring of diameter making of curve Calculation of sample concentration

SRID:Single Radial Immuno diffusion or Mancini

SRID Plate

Single Radial Immuno diffusion :

In SRID :34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4

Gel is mixed with Ab

Ag is in punch In Single Radial Diffusion: one component is fixed

(e.g. antigen-in this case it is IgG)

As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as

illustrated in Figure

The diameter of the ring is proportional to the concentration of Ag .

(Double Immuno Diffusion =)DID or

Ouchterlony ImmunodiffusionQualitative2 DiffusionApplication in research laboratoryIs not as a routin method now

Ouchterlony - Identity

Ouchterlony – Partial Identity

Ouchterlony Non-Identity

Turbidimetery

measuring the amount of light Passing Through the solution.

Nephelometery

Measures SCATTERED light

Immunoelectrophoresis

Counter current IEP : Qualitative Rocket IEP: Quantitative

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