Faculty of Chemistry University of Belgrade

Center for Chemistry

Institute for Chemistry,

Technology and Metallurgy

Belgrade

Laboratory for Instrumental Analysis (founded 1966), former Center for Instrumental Analysis = “CIA”

accredited to ISO 17025 standard

Studentski trg 12 - 16 Belgrade, Serbia

Prof. Milutin

Stefanović

1924 - 2009

Prof. Dragoslav

Jeremić

Dr. Vlatka Vajs

Dr. Vele Tešević

Dr. Nina Todorović

Dr. Snežana Trifunović

Dr. Ivan Vučković

Dr. Dejan Gođevac

Miroslav Novaković,

M. Sci.

Milka Jadranin, M.Sci.

Danica Bajić

Marina Blagojević

Dejan Vučetić

Dr. Iris Djordjević

Dr. Ivana Aljančić Boris Mandić, M.Sci.

Ljubodrag Vujisić,

M.Sci. Boris Mandić, M.Sci.

Dr. Slobodan Milosavljević

LC/MS ESI TOF

500 MHz NMR FT IR

GC/MS

Equipment of CIA

Activities of CIA:

1. Service: running spectra, chromatograms and microanalysis interpretation of spectra miscellaneous (Police, Medicine and Medical Devices

Agency of Serbia)

UV

NMR

IR

2. Teaching: • “Structural instrumental methods” • “Chemistry of secondary metabolites”

O

CH3

Cl

Example of an exam problem for

a third-year student of chemistry

MS

13C NMR

1H NMR

UV

IR

3. Phytochemistry Scientific project (No 172053, Ministry of Science and Technological Development, Serbia): “Bioactive natural products of wild-growing, cultivated and edible plants: structure and bioctivity determination”

Mountain Orjen, Montenegro 2006

Photo smilo

Dr. Vlatka Vajs

•Institute for Medicinal Plant Research “Dr Josif Pančić", Belgrade

•Botanic Garden Dulovine, Kolašin, Montenegro

•Mayo Clinic Rochester, Minnesota, USA

•Faculty of Biology, University of Belgrade

•Faculty of Pharmacy, University of Belgrade

•Institute for Biological Research “Siniša Stanković”, Belgrade

•Faculty of Natural Sciences and Mathematics, Niš

•School of Medicine, University of Belgrade

•The Institiute for Oncology and Radiology, Belgrade

•The Institute for Nuclear Sciences, Vinča

•Faculty of Science, Podgorica

•National Park, Galičica, Ohrid

Collaboration with the following institutions :

Joint project of Serbian Academy of Sciences and Arts

and Bulgarian Academy of Sciences: "Natural products

from plants and marine organisms: medicinal and

ecological significance".

1. Asteraceae: Achillea (11), Ambrosia (1), Amphoricarpos (2), Anthemis

(6), Artemisia (7), Centaurea (8), Cicerbita (2), Eupatorium (1), Inula (3),

Senecio (7), Ptilostemon (2), Tanacetum (6), Telekia (1)

2. Apiaceae: Angelica (1), Athamanta (1), Cherophyllum (1), Laserpitium

(4), Peucedanum (1), Seseli (3)

3. Gentianaceae: Gentiana (2), Gentianella (5), Swertia (1)

4. Guttiferae: Hypericum (4)

5. Lamiaceae: Lycopus (1), Phlomis (1)

6. Dipsacaceae: Cephalaria (3)

7. Boraginaceae: Rindera (1)

Phytochemical investigations: studied genera

initiated by M. Stefanović and D. Jeremić by the

beginning of seventies

Artemisia annua L. O

O

OO

O

H

H

Artemisinin

8. Anacardiaceae: Cotinus (1)

Serbia and Montenegro

•Galičica, Ohrid

Macedonia

Phytochemistry

•Tara mountain

•Montenegro mountains

•Šar-Planina, (Šara Mountain)

•Sićevačka klisura (Sićevo

gorge)

•Stara Planina (Old mountain)

•Deliblatska peščara (Deliblato

sand)

Sesquiterpene

lactones

O

O

RO

H

H

OH

R1

O

OH

MeO

MeO

R3'

OR4'

O

OMe

Flavonoids

O

O

R5

OR8

R3O

OR1

R7

O

O

OGlc

H

O

CH2OH

R1O

O

OR2

R1= β-D-Xyl(1-3)- α-L-Rha(1-2)- α-L-Ara-

R2= β-D-Glc-

Triterpenoid

saponins

Xanthones Secoiridoids H H

O

OHO

O

1

23

4

5

6

78

9

10

1'

2'

3'

4'

5'

6'

1" 2"

3"

1''' 2'''

3'''

4'''5'''

4"

Prenylated

phloroglucinols N

OO

1R

O

R2O

O OO

OMe

Coumarins Pyrrolizidine alkaloids

Compounds

1. Antibacterial

2. Antifungal

3. Cytotoxic

4. Тubuline test

4. Radioprotective

5. Antioxidative

Bioactivities

Recent topics

Grape seeds

Vitis vinifera

Еxtr.: EtOAc + H2O (10%)

LC/UV/ESI-TOF MS 13C NMR

17 polyphenols

Antioxidants in healthy food of Balcans

HPLC of grape seed

extract

13C NMR of grape seed

extract

M. Stanković et al., "Antioxidant properties

of grape seed extract on human lymphocyte

oxidative defence", Planta Medica, 74, 730-

735 (2008)

1. R. Matović, R. N. Saičić: An efficient semisynthesis of 7-deoxypaclitaxel

from taxine, J. Chem. Soc., Chem. Commun. 1998, 1745-1746.

2. R. N. Saičić, R. Matović, Ž. Čeković: Semisynthesis of Taxol: an

improved procedure for the isolation of 10-deacetylbaccatin III, J. Serb.

Chem. Soc. 1999, 64, 497-503; Natural Products Updates 2000,

34343.

3. R. N. Saičić, R. Matović: An efficient semisynthesis of 7-deoxypaclitaxel

from taxine, J. Chem. Soc., Perkin Trans. 1, 2000, 59-65.

4. Z. Ferjančić, R. Matović, Z. Čeković, J. P. Snyder and R. N. Saičić:

Synthesis, biological evaluation and modeling of a C,D-seco-taxoid,

Tetrahedron Lett. 2005, 46, 5049-5052.

5. Z. Ferjančić, R. Matović, Ž. Čeković, Y. Jiang, J. P. Snyder, V. Trajković

and R. N. Saičić: Synthesis, biology and modeling of C-4 carbonyl C,D-

seco-taxoid, Tetrahedron 2006, 62, 8503-8514.

6. Z. Ferjančić, R. Matović, Ž. Čeković and R. N. Saičić: Reactions of -

4(20)-epoxy-5-O-mesyltriacetyltaxicine I induced by BF3•Et2O/Bu4NBr,

J. Serb. Chem. Soc. 2006, 71, 705-711.

dr. Radomir N. Saičić

Organic Synthesis

A view of Prokletije mountains (over Plav lake), Montenegro

Photo smilo

The End