Laboratory Work: IMNV diagnostics field and laboratory protocols(PCR, histopathology)

Asian Regional Laboratory Testing ProgramFor Aquatic Animal Diseases 2013-2014

OIE Reference Laboratorfor White spot disease

OIE Reference Laboratorfor Infectious hypodermal and haematopoietic necrosis

Sources of samples:1. Positive control came from Indonesia, IMNV infected P.vannamei2. Negative control came from USA, SPF P.vannamei

Sample I.D.

Clinical sign

Scale

General information of the sample received in our regular detection test

Step1: Sample preprocessing

Before

Homogenate

After

Nucleic Acid Automatic Extraction System

Step2: Extract nucleic acid

Step2: Extract nucleic acid

Nucleic Acid Automatic Extraction System

Step3: Molecular techniques

First round

Second round

RT-PCR

Step3: Molecular techniques

qRT-PCR

Analytik Jena

Step3: Molecular techniques

qRT-PCR

Thank you