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Laboratory Work: IMNV diagnostics field and laboratory protocols (PCR, histopathology)
Laboratory Work: IMNV diagnostics field and laboratory protocols(PCR, histopathology)
Asian Regional Laboratory Testing ProgramFor Aquatic Animal Diseases 2013-2014
OIE Reference Laboratorfor White spot disease
OIE Reference Laboratorfor Infectious hypodermal and haematopoietic necrosis
Sources of samples:1. Positive control came from Indonesia, IMNV infected P.vannamei2. Negative control came from USA, SPF P.vannamei
Sample I.D.
Clinical sign
Scale
General information of the sample received in our regular detection test
Step1: Sample preprocessing
Before
Homogenate
After
Nucleic Acid Automatic Extraction System
Step2: Extract nucleic acid
Step2: Extract nucleic acid
Nucleic Acid Automatic Extraction System
Step3: Molecular techniques
First round
Second round
RT-PCR
Step3: Molecular techniques
qRT-PCR
Analytik Jena
Step3: Molecular techniques
qRT-PCR
Thank you
