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NATO

Science for Peace and Security (SPS) Programme

Workshop on CBRN Defence – 22-24 October 2013 – Brussels

Emerging Security Challenges Division

NATO

Brussels, Belgium, 22.10.2013

Antibacterial coatings based on sonochemically

designed surface metal nanofoams, 2011, CLG

984267

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Antibacterial coatings based on sonochemically designed surface metal

nanofoams, 2011, CLG 984267

• General overview: Advances for a new generation of active metal surfaces with

feedback properties.

• Objectives: Technologies for fabrication of surface metal nanofoams for active

chemicals storage by “green” ultrasound irradiation with combination with self-

assembly complexation between biocide and polyelectrolyte in open/close states

and formation of surface nanoreservoirs capable of long-term storage and

controllable release of the entrapped antimicrobial agents which provide a basis for

novel CNBR decontamination coatings.

• Expected outcomes comprehensible to the non-expert: fabrication of

surface encapsulation systems capable of long-term storage and controllable

release of the entrapped antimicrobial agents which provide a basis for novel CNBR

decontamination coatings.

Project Description

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What did we want to achieve ?

Rational interface design

inorganic / polymeric / biological

→ antifouling systems, new medical therapies for human transplants, stem-cell research

Understanding and controlling of sonochemical reaction at solid surfaces

→ boost fundamental research, control of nucleation

Nanoengineered Cavitation Interfaces

Bio-active Surface

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What is special in sonochemistry?

High Energy

Fast

Localised

Sonoparameters → easy way to various structures

Red/Ox vis-à-vis reaction in liquid

Additive in liquid → single-step hybrids

“Hot spot” theory

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Focused ultrasonic treatment

* Lower left inset shows multibubble sonoluminecence

** Right inset shows ultrasonic unit for sonochemical modification, where 1) piezoceramic transducer; 2) booster; 3)

irradiating horn; 4) gas inlet; 5) thermoelement; 6) thermostated sonocell.

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Surface capsules could be formed at higher intensity of sonication

Cavitation assisted porous surfaces

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Surface capsules on metal surface

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Design of intelligent surfaces

TEM ultramicrocut of sonomodified metal

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Types of capsules

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Surface pore loading

“Surface” drug delivery: stimuli-responsive, controlled, extended, multi-drug, with biosensing

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Encapsulation forces

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Controllable release behavior

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Specific example of the system

Doxorubicin chemisorption via sonication

IR spectra

D.V. Andreeva, D.V. Sviridov, A. Masic. H. Möhwald, E.V. Skorb, SMALL 8 (2012) 820

Fast creation of reactive groups on both inorganic and organic sides

Release profile

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Specific example of the system

D.V. Andreeva, D.V. Sviridov, A. Masic. H. Möhwald, E.V. Skorb, SMALL 8 (2012) 820

Release profile

before biocide release

after biocide release

LIVE/DEAD BacLight bacteria visualisation: inactivated – red; living – green

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J. Gensel, T. Borke, N. Pazos- Perez, A. Fery, D.V. Andreeva, E. Betthausen, A. H. E. Müller, H. Möhwald,

E.V. Skorb, Adv. Mater. 24 (2012) 985

Immobilization of pH-responsive triblock terpolymer micelles

L. lactic bacteria produce lactic acid, pH ↓, micelle’s corona size ↑ and push off the cells

Spatial and temporal resolved cell attraction

Advances of self-regulated system

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Project Outcome

Antibacterial coatings based on sonochemically designed surface metal

nanofoams, 2011, CLG 984267

• Results (direct scientific and educational successes):

Novel ultrasound-assisted method of modification of metal surface and

formation of metal nanofoams with high stability, high surface area and high

adhesive properties;

Novel encapsulation systems with controllable release of biocide and other

active compounds;

5 publications in high ranking scientific journals in the field of materials science

and nanotechnology, e.g. Adv. Mater., Nanoscale, Small, J. Mater. Chem.,

Photochem. Photobiol. Sci.

• Impact :

sustainable technology for novel CRBN decontamination coatings;

potential application in protective coatings, drug delivery, implants protection,

tissue engineering, lab-on-chip, sensors and biosensors.

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Antibacterial coatings based on sonochemically designed surface metal

nanofoams, 2011, CLG 984267

• What research in CBRN Defence requires urgent and substantial

attention? A novel nanoengineered systems for manipulation with cells in

the bioanalytic lab-on-chip systems.

• What should be the focus of the SPS Programme in CBRN

Defence? Use advances of novel nanoengineered materials, systems

fundamental understanding and technological adaptation.

• Ideas for potential SPS activities in the CBRN field (according to

your expertise) Hybrid materials for encapsulation systems, stimuli-

responsive surfaces, CBRN decontamination coatings.

Way Forward – SPS CBRN