Post on 19-Mar-2018
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NucleoCounter® NC-250™ The Fast and Precise Cell Counter
NucleoCounter® NC-250™
The NucleoCounter® NC-250™ is an integrated fluorescence mi-croscope with dual fluorescence channels designed to detect signals from cells stained with fluorescent dyes.
Performing multiple determinations of viability and cell count is fast and easy with the NucleoCounter® NC-250™. Process 8 samples in one run. Just prepare your cell suspensions by add-ing the staining solution containing fluorescent dyes, load the 8 cell samples into one slide and press RUN.
The NucleoCounter® NC-250™ also provides easy, fast (5 minutes) and high precision assaying of cell cycle profiles.
In addition, you can measure cell health with our quick and reli-able apoptosis assay. VitaBright-48™ stain instantly becomes fluorescent when it binds to thiols inside cells. The level of thiols correlates positively with cell health. A reduction in the level of reduiced thiols in a cell is a documented event in apoptosis.
The NucleoCounter® NC-250™ is a compact benchtop instrument which fits perfectly into any cell laboratory.
Key Benefitsof the NucleoCounter® NC-250™
8 viability and cell counts in less than3 minutes
- Total cell count- Viability- Cell diameter- % of aggregated cells
Up to 8 advanced analyses in one run- High precision cell cycle analysis- Cell health determination with the fast apoptosis assay
Fast and easy operation
Excellent reproducibility
No calibration
Maintenance and service free
Automated PDF reports
21 CFR Part 11 ready
The NucleoCounter® NC-250™ 8 samples in one run!
Vaccine Production | Micro-Carriers | Stem Cells | Cancer Cells | CEF Cells | Toxicology | PBMC | and more
990-
0254
ver
. 02.
2016
www.youtube.com/chemometec
Three useful Assays
NucleoCounter®
NC-250™
Loading volume: 30 μl for A2-slides, 10 μl for A8-slides
Measurement volume: Viability and Cell Count Assay: 11.2 μl for
A2-slides, 2.4 μl for A8-slides
Analysis time: Viability and Cell Count Assay:
3 minutes for 8 samples in one A8-slide
2 minutes for 2 samples in one A2-slide
Measurement range: Viability and Cell Count Assay: 5 x 103 to
1 x 107 cells/ml
Optimal range: Viability and Cell Count Assay: 5 x 104 to
5 x 106 cells/ml
Size: 19 x 29 x 32 cm / 8 x 12 x 13 in. (W x H x D), weight 9 kg / 20 lb
Software: NucleoView™ NC-250 software
NucleoCounter® NC-250™ Specifications
Viab
ility
and
Cel
l Co
unt
Assa
y
Viability and Cell Count Assay
Mix cell sample with the staining solution, load the slide and press RUN.
Get 8 results in 3 minutes with total cell count, viability, cell diameter and percentage of aggregated cells.
Cell
Cycl
e As
say Cell Cycle Assay
Incubate cell sample for 5 minutes at 37°C with a mixture of staining solution and lysis buffer, neutralize by adding stabilizing buffer, load slide and press RUN. Get 8 high precision cell cycle profiles in 4 minutes.
Vita
lity
Assa
y Vitality Assay
Add VitaBright-48™ to your sample, immediately load the slide and press RUN.
Get 8 apoptosis measurements in 4 minutes.
For more information, please visit www.chemometec.com/NC-250
ChemoMetec Inc.630 Pena DriveDavis, CA 95618,USA
Phone: (888) 988-8542 Mail: contact@chemometec.comWeb: www.chemometec.com
Next generation cell analysis is here:NucleoCounter® NC-3000™
Automated PDF reports
G & G Instruments Kft.H 1182 Bp. Himesháza u. 12.Phone: +36 (1) 297-4000 / 296-0562Fax: +36 (1) 297-4001Mail: info@gandginstruments.huWeb: www.gandginstruments.hu