Prokaryotic Biology - The Key to Understanding The Complexity of...

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63rdAnnualWindRiverConferenceonProkaryoticBiology

Prokaryotic Biology

- The Key to Understanding The Complexity of Life -

June5-9,2019

DaoHouseEstesPark

ColoradoUSA

63rdAnnualWindRiverConference,June5-9,2019

WELCOMETOTHE63rdANNUALWINDRIVERCONFERENCEONPROKARYOTICBIOLOGY2019

WindRiverExecutiveCommittee:• Dr.HelenWing,UniversityofNevada,LasVegas• Dr.ErinMurphy,OhioUniversity• Dr.EthanMann,SharkletTechnologies,Inc.• Dr.MarioPedraza-Reyes,UniversityofGuanajuato• Dr.EduardoRobleto,UniversityofNevada,LasVegas• Dr.CorrieDetweiler,UniversityofColoradoBoulder• Dr.R.Martin(Marty)Roop,EastCarolinaUniversity • Dr.DanielWozniak,OhioStateUniversity • Dr.MichaelSchurr,UniversityofColoradoAnschutzMedicalCampus • Dr.AmandaOglesby-Sherrouse,UniversityofMaryland

Scientificprogramchair:

• Dr.ClaytonCaswell,VirginiaTech Contributions from the following sponsors helpedmake this year’s conference possible and are gratefullyacknowledged:MonserateBiotechnologyGroupSharkletBiotechnologies,Inc.TheDaoHouseUNLV,SchoolofLifeSciencesSpecialacknowledgement:

• Special thanks are due to Rosy Moloche Garcia at UNLV for expert administrativeassistance.

• Specialthankstoalltraineeswhovolunteeredtheirtimetohelpwiththemeeting.• Special thankstoEduardoRobletoandHelenWingatUNLVfor invaluableacademicand

administrativesupport.• SpecialthankstoEduardoRobletoforgrantwritingactivities.

THEWINDRIVERHALLOFFAME:PASTANDPRESENTPLENARYSPEAKERS2019 Dr.ChristineJacobs-Wagner YaleUniversity

Dr.ElizabethRucks UniversityofNebraskaMedicalCenterDr.ClayFuqua IndianaUniversityBloomingtonDr.ChristopherWaters MichiganStateUniversity

2018 Dr.DeniseM.Monack StanfordUniversity Dr.AnthonyMaurelli UniversityofFlorida,EmergingPathogensInstitute Dr.AmyVollmer SwarthmoreCollege Dr.AlfredoTorres UniversityofTexasMedicalBranchatGalveston2017 Dr.MelodyMills NIH,NIAID

Dr.CatherineDrennan MassachusettsInstituteofTechnologyDr.MatthewParsek UniversityofWashingtonDr.ForestRohwer SanDiegoStateUniversity

2016 Dr.GrahamWalker MassachusettsInstituteofTechnology Dr.EvgenyNudler NYUSchoolofMedicine Dr.VictorNizet UniversityofCalifornia,SanDiego Dr.PeterSetlow UniversityofConnecticutHealthSciencesCenter2015 Dr.VictorTorres NYUSchoolofMedicine Dr.NancyFreitag UniversityofIllinois-Chicago Dr.DavidWeiss EmoryUniversity Dr.FranzNarberhaus RuhrUniversity2014 Dr.MichaelGilmore HarvardMedicalSchool

Dr.GiselaStorz NationalInstitutesofHealth Dr.FerranGarcia-Pichel ArizonaStateUniversity Dr.BarbaraFunnell UniversityofToronto2013 Dr.NinaSalama FredHutchinsonCancerInstitute Dr.ShelleyPayne UniversityofTexasatAustin Dr.ReneeTsolis UniversityofCaliforniaDavis Dr.RalphIsberg TuftsUniversity2012 Dr.DavidRudner HarvardMedicalSchool Dr.EstherAngert CornellUniversity Dr.MarkGomelsky UniversityofWyoming Dr.DennisA.Bazylinski UniversityofNevada,LasVegas2011 Dr.JamesBardwell UniversityofMichigan Dr.SeanCrosson UniversityofChicago Dr.JoHandelsman YaleUniversity Dr.R.MartinRoop,II EastCarolinaUniversity2010 Dr.PeterChristie UniversityofTexas-HoustonMedicalSchool

Dr.RichardGourse UniversityofWisconsin Dr.DanKearns UniversityofIndiana

Dr.RolfThauer MaxPlanckInstituteforTerrestrialMicrobiology,Marburg2009 Dr.JimImlay, UniversityofIllinoisatUrbana-Champaign Dr.AndyCamilli TuftsUniversity,HowardHughesMedicalInstitute Dr.JadeWang BaylorCollegeofMedicine Dr.NormanPace UniversityofColoradoatBoulder2008 Dr.RobertoKolter, HarvardMedicalSchool

Dr.GrahamHatfull, UniversityofPittsburgh Dr.RobertoBelas, UniversityofMaryland

Dr.WilliamJacobs, AlbertEinsteinCollegeofMedicine2007 Dr.JaredLeadbetter, CaliforniaInstituteofTechnology Dr.TinaHenkin, OhioStateUniversity Dr.HarryMobley, UniversityofMichiganMedicalSchool Dr.LynnZechiedrich, BaylorCollegeofMedicine2006 Dr.DavidFigurski, ColumbiaUniversityMedicalCenter Dr.KarlE.Klose, UniversityofTexas-SanAntonio Dr.JohnHelmann, CornellUniversity Dr.VictorDirita, UniversityofMichiganSchoolofMedicine2005 Dr.SamuelKaplan, UniversityofTexasHoustonMedicalSchool Dr.GeorgeO’Toole, DartmouthMedicalSchool Dr.DianneNewman, CaliforniaInstituteofTechnology Dr.TomSilhavy, PrincetonUniversity2004 Dr.CurtisSuttle, UniversityofBritishColumbia Dr.PaulGollnick, StateUniversityofNewYork-Buffalo Dr.HeidiKaplan, UniversityofTexasHoustonMedicalSchool Dr.JorgeGalán, YaleUniversitySchoolofMedicine2003 Dr.VincentFischetti, RockefellerUniversity Dr.RobertLandick, UniversityofWisconsin Dr.BonnieBassler, PrincetonUniversity Dr.NeilWelker, NorthwesternUniversity2002 Dr.StephenFarrand, UniversityofIllinois-Urbana Dr.BarryWanner, PurdueUniversity Dr.SanfordA.Lacks, BrookhavenNationalLaboratory Dr.JanetYother, UniversityofAlabama-Birmingham Dr.MalcolmWinkler, LillyResearchLaboratories2001 Dr.KimLewis, TuftsUniversity Dr.AustinNewton, PrincetonUniversity Dr.JulieTheriot, StanfordUniversity Dr.DaleKaiser, StanfordUniversity2000 Dr.ThomasA.Trautner, Max-PlanckInstituteforMolecularGenetics Dr.RylandF.Young, TexasA&MUniversity Dr.AbigailA.Salyers, UniversityofIllinois Dr.JohnW.Costerton, MontanaStateUniversity Dr.TonyRomeo, UniversityofNorthTexasHealthScienceCenter1999 Dr.FrederickNeidhardt, UniversityofMichigan Dr.OlafSchneewind, UniversityofCaliforniaatLosAngeles Dr.RogerW.Hendrix, UniversityofPittsburghBacteriophageInstitute Dr.EugeneNester, UniversityofWashington1998 Dr.ScottHultgren, WashingtonUniversitySchoolofMedicine Dr.SusanM.Rosenberg, BaylorCollegeofMedicine Dr.MaryLouGuerinot, DartmouthCollege Dr.PeterGreenberg, UniversityofIowa

1997 Dr.MalcolmWinkler, UniversityofTexasHoustonMedicalSchool Dr.JohnBaross, UniversityofWashington Dr.PhilipHarriman, NationalScienceFoundation Dr.SusanGolden, TexasA&MUniversity

Welker-StreipsMemorialPlenaryAddress

NeilE.Welkerleftusafterashort,butbrave,battlewithcanceronWednesday,Aug.11,2004atage72.Neil,borninDarienCenter,NYonApril21,1932,receivedhisPhDfromWesternReserveinCleveland,OHandmovedtotheChicagoareawherehewasaProfessorofBiochemistry,MolecularBiologyandCellBiologyatNorthwesternUniversityinEvanston,ILfrom1964topresent.Heisnotedforpromotingexcellenceinundergraduatescienceeducationandsupportingstudents'participationintheWindRiverConferenceeachyearinColorado.Heissurvivedbyhislovingwife,FatimaVilich,andherchildren,InesandAdisKurtovic;histhreechildren,ThomasandDouglasWelkerandJenniferBurgerandtheirspousesandchildren;histhreestepchildren,DonandJonPollackandJoAnnCarbrayandtheirspousesandchildren;hissister,ArleneCoccariandbrother,WallaceWelkerandtheirspouses,childrenandgrandchildren;andnumerousniecesandnephews.

ThispastFebruary,TheWindRiverConferenceonProcaryoticBiologylostafriendandanactivesupporter/leader.Dr.UldisStreipshadbeenthelong-timeExecutiveDirectoroftheConferenceandworkedtirelesslyfrom1968to2016tokeeptheconferencemovingforwardasaprinciplemechanismfortheexchangeofcuttingedgescienceandasaplacewherestudentswouldbeofprimaryimportance.

UldiswasBorninRiga,LatviaonFebruary1,1942.In1944,Uldis,hisparentsHugoandElenoraStreips,andolderbrotherLaimonis,fledfromtheirhomelandduetowar.Withnowheretogo,hisfamilylivedindisplacedpersonscampsinGermanyandPolandforsevenyears.TheycametotheUSin1951andeventuallysettledinChicago.HegraduatedValparaisoUniversityin1964withaB.A.inBiologyandChemistryandthenenteredNorthwesternUniversitywhereheworkedonhisPh.D.inthelaboratoryofDr.NeilWelker(oneoftheoriginalparticipantsintheWindRiverConferencewhichwascalledTheTransformationMeetings).Uldisreceivedhisdegreein1969andthenwenttodoapostdoctoralfellowshipinthelaboratoryofDr.FrankE.Young(anotherfoundingmemberoftheTransformationMeetings)atScrippsClinicandResearchFoundation.UldiswhotrulyadoredlifeinSanDiegowasdealtacrueltwistoffatewhenFrankYoungacceptedtheChairmanshipoftheDepartmentofMicrobiology&ImmunologyattheUniversityofRochester,SchoolofMedicineandDentistry.UldishadtoturninplayingvolleyballintheeveningsonthebeachfortheweatherofupstateNY.Luckily,UldiswasrewardedbymeetinghisfuturewifePatriciaWilliamswhoworkedintheDepartmentinRochester;heimmediatelystoppedcomplainingabouttheweather.In1972,UldisbecameanAssistantProfessorofMicrobiologyattheUniversityofLouisville,MedicalCenter.Becauseofhisstate-of-theartresearchandhisoutstandingcommitmenttostudents,heproceededtobepromotedtoAssociateProfessor(1978)andProfessor(1988).HeremainedatULuntilhisretirementtwoyearsago.

Uldisreceivedseveralteachingawards,aswellastheAMWAGenderEquityAward.HewastherecipientofmultipleresearchgrantsandservedasViceChairmanoftheDepartmenttwice(forover10years).Heauthored61peerreviewedjournalarticlesand75chapters,booksandproceedings.Hewasapeerreviewerandaneditor.Hiscommitmenttomedicalstudentandgraduatestudenteducationwasspecificallyrecognizedbyhisuniversity.ThiscommitmenttostudentscarriedovertotheleadershipandguidanceheprovidedduringhisentirecareertotheWindRiverConference.Hemadesurethatstudentswerealwaysfirstandcenterinallconsiderationsandplanningfortheconference.EverythingUldisdidhedidwithpassionandcommitment.Thiswasdemonstratedmostclearlybyhis

commitmenttohisfamily;hiswifePat,hisdaughterKrista,hisson,daughter-inlawandgrandchildrenKrisjans,Sarah,Anja,Zander,Nora&Maia,respectively.UldisneverforgothisLatvianheritageorisloveofmusic.HeworkedtohelpLatviabeforeandafteritgainedindependencefromtheSovietUnionandwasrecognizedforhistirelessworkbybeingmadeamemberoftheLatvianAcademyofScience.Hewasthementorto17M.S.anddoctoralstudentswhotrainedunderhimwhilehewasatUL.Healsoprovidedguidancetocountlessnumberofmedicalstudents.For40years,Uldiswasaproud,loyalbassguitarist,vocalist,andcomedianforthebandCikagasPiecisi,asit

grewfromitsLatvianrootsintointernationalfame.Asmentionedabove,thispastFebruarywelostagreatfriend,scientist,teacher,mentor,LatvianScholar,musicianandmostofallawonderfulhusband,fatherandgrandfather.Uldiswastrulyamenschandheismissed.

Wednesday,June5,20193:00-6:00PM

Registration

4:00-6:30PM

WelcomeReception(Lobbyofmainlodge)

6:30-8:00PM

Dinner&Openingremarks

8:00-9:00PM

KeynoteAddress:

GoverningandorganizationalprinciplesofgeneexpressionDr.ChristineJacobs-Wagner

YaleUniversityHowardHughesMedicalInstitute

WestHaven,CT

SessionConvener:CassandraNelson

9:00PM- HappyHourattheloungeandbar

Thursday,June6,20197:00-8:15AM

Breakfast(GreatRoomDiningRoom)

8:15AM8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM

GeneralAnnouncementsMicrobialBiochemistryandPhysiologyISessionConvener:AmberConsulEffectsofP.aeruginosa2-heptyl-4-quinoloneN-oxide(HQNO)onglobalironregulationinS.aureusLukeK.BrewerUniversityofMarylandBaltimore,Baltimore,MD,USAUnderstandingcorrinoidsalvaginginhyperthermophilicArchaeaElizabethA.VillaUniversityofGeorgia,Athens,GA,USACyclicdi-GMPcontrolsstresstoleranceandcellshapeinVibriocholeraeNicolasL.FernandezMichiganStateUniversity,EastLansing,MI,USA

PolarorganelledevelopmentproteinPodJisimportantforthesynthesisofoneofthetwodistinctunipolarpolysaccharideadhesinsinAgrobacteriumtumefaciensMaureenC.OnyeziriIndianaUniversity,Bloomington,IN,USA

63rdAnnualWindRiverConference,June5-9,2019 9

9:30-9:45AM9:45-10:00AM

LossofYggSinducesaconditionalaspartatelimitationinaspCstrainsofSalmonellaentericaHuongN.VuUniversityofGeorgia,Athens,GA,USA

Alinkbetweenhigh-affinityzinctransport,capsuleproduction,andgrowthofextraintestinalpathogenicEscherichiacoliinbileMichaelOlsonBrighamYoungUniversity,Provo,UT,USA

10:00-10:30AM CoffeeBreakMicrobialBiochemistryandPhysiologyIISessionConvener:KellieKing

10:30-10:45AM10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:0012:15-1:15PM

ThestressoftransitioningtomethylotrophyJannellV.BazurtoUniversityofMinnesota,St.Paul,MN,USAResponseofPseudomonasaeruginosatocalprotectin-mediatedmulti-metalsequestrationCassandraE.NelsonUniversityofMarylandBaltimore,Baltimore,MD,USAMinsystemdisassemblesFtsZatcellpoleinBacillussubtilisYuanchenYu

IndianaUniversity,Bloomington,IN,USABiochemical characterization of the cyclic-GMP-AMP dependent activation of thephospholipaseCapVGeoffreyB.SeverinMichiganStateUniversity,EastLansing,MI,USACobamidesynthaseanchorsthelatestepsofcobamidebiosynthesistothecellmembraneVictoriaL.JeterUniversityofGeorgia,Athens,GA,USADegenerateformaldehydeoxidationpathwaysinMethylobacteriumextorquenscausedifferentialepistaticeffectsbetweenmutationsaccumulatedduringexperimentalevolutionEricL.BrugerUniversityofIdaho,Moscow,ID,USALunch(GreatRoomDiningRoom)

1:30-1:45PM1:45-2:00PM2:00-2:15PM2:15-3:45PM

ResponsestoEnvironmentalStimuliandEcologySessionConvener:GarettBondsEnterococcusfaecalis:TakingupfatfromitshabitatRachelD.SteeleUniversityofTennessee,Knoxville,TN,USALegume-rhizobiasymbioticoutcomeasdeterminedbyaninterkingdompeptide-peptidasecontestAlexB.BenedictBrighamYoungUniversity,Provo,UT,USAUnderstandingthestresstoleranceofSaccharomycescerevisiaestrainsevolvedforhighsolidspinefermentationsOliviaA.ThompsonUniversityofGeorgia,Athens,GA,USA15LightningTalks:Fast-pacedpresentationstowhetyourappetitefortheupcomingpostersessions.

3:45-4:30PM4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-

FreeTime&PosterSet-upPosterSession&HappyHour(Oddnumberedposters)Dinner(GreatRoomDiningRoom)

KeynoteAddress:MappingtheChlamydiatrachomatisinclusionmembranewithproximitylabeling

systemsDr.ElizabethRucks

UniversityofNebraskaMedicalCenterOmaha,NE

SessionConvener:GeoffreySeverin

HappyHourattheloungeandbar

Friday,June7,2019

7:00-8:15AM

Breakfast(GreatRoomDiningRoom)

8:15AM

GeneralAnnouncements

8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM9:30-9:45AM9:45-10:00AM

GeneRegulationandBacterialGeneticsISessionConvener:RaevenBastockInvestigatingthenon-canonicalroleofthetranscription-couplingrepairfactorondisulfidestressinBacillussubtilisHollyA.MartinUniversityofNevada,LasVegas,NV,USATranscriptomicprofilingofbacteriophage-hostinteractionsrevealsphagemediatedquorumquenchingofEnterococcusfaecalisAnushilaChatterjeeUniversityofColoradoDenver,Aurora,COUSAMini-Tn7vectors,universaltoolsforgeneticmanipulationofGram-negativebacteriaHerbertB.SchweizerUniversityofFlorida,Gainesville,FL,USA

Thecyclicdi-GMPdegradationproductpGpGactivatesvirulenceinErwiniaamylovoraBrianY.HsuehMichiganStateUniversity,EastLansing,MI,USAThe activation of the extracytoplasmic function (ECF) σ factor, σP, is a regulatedprocessthatoccursinresponsetothepresenceofβ-lactamsKelsieM.NautaUniversityofIowa,IowaCity,IA,USA

Anovelhigh-throughputmethodforidentifyingmetabolicpathways,Metabolite-coupledTn-Sequencing(MetSeq),revealsnewironandhemeregulationinPseudomonasaeruginosaAndrewT.UlijaszLoyolaUniversityChicago,Maywood,IL,USA

10:00-10:30AM CoffeeBreakGeneRegulationandBacterialGeneticsIISessionConvener:LukeBrewer

10:30-10:45AM

Identification,characterizationandcomparisonofRNAthermosensorsinShigelladysenteriaeandStaphylococcusaureusErinR.MurphyOhioUniversityHeritageCollegeofOsteopathicMedicine,Athens,OH,USA

10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:0012:00-12:15PM12:30-1:30PM1:30-4:30PM

SwrAregulatesswarmingmotilitybyactivatingmotilityrelatedgeneswithintheDegUregulonAyushiMishraIndianaUniversity,Bloomington,IN,USATheroleoftranscriptioninpilinantigenicvariationofthepathogenicNeisseriaHankSeifert

NorthwesternUniversityFeinbergSchoolofMedicine,Chicago,IL,USADissectingtheregulationofthePseudomonasaeruginosahemeassimilationsystem:AlinkbetweenhememetabolismandregulationSusanaMouriñoUniversityofMaryland,Baltimore,MD,USA

H-NSfamilymembersMvaTandMvaUregulatethePseudomonasaeruginosatypeIIIsecretionsystemEmilyA.WilliamsMcMackinUniversityofIowa,IowaCity,IA,USACharacterizationoftheiron-andheme-responsivesRNAPrrHinPseudomonasaeruginosaTramyHoangUniversityofMaryland,Baltimore,MD,USAPhotograph!Lunch(GreatRoomDiningRoom)Freetime

4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-

PosterSession&HappyHour(Evennumberedposters)Dinner(GreatRoomDiningRoom)

KeynoteAddress:Pterinitup:Howasmallobscuremetabolitecontrolssurfaceattachmentby

AgrobacteriumtumefaciensDr.ClayFuqua

IndianaUniversityBloomington,IN

SessionConvener:ElizabethVilla

Saturday,June8,20197:00-8:15AM8:15AM

Breakfast(GreatRoomDiningRoom)GeneralAnnouncementsHost-PathogenInteractionsSessionConvener:EmilyMcMackin

8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM9:30-9:45AM9:45-10:00AM

EnterococcalpolysaccharideantigenimpactstransmissionfrommothertoinfantCydneyN.JohnsonUniversityofColoradoAnschutzMedicalCampus,Aurora,CO,USAAn‘openchannelconfirmation’VirB10deregulatesintracellulartraffickingandvirulenceofBrucellasuisDavidO’Callaghan

UniversitédeMontpellier,Nimes,FranceTheroleofbiofilmformationandpolyamineutilizationincolonizationofzebrafishbyVibriocholeraeGarettBondsAppalachianStateUniversity,Boone,NC,USAEvolutionofPseudomonasaeruginosainachronicburnwoundmodelErinS.GloagTheOhioStateUniversity,Columbus,OH,USAElucidatingthehealthyratoralbacteriomeandmycobiomeSaredaT.J.SchrammTheUniversityofTexasatDallas,Richardson,TX,USADiscoveryandcharacterizationofnovelsmallregulatoryRNAsinBrucellaabortusKellieA.KingVirginiaTech,Blacksburg,VA,USA

10:00-10:30AM10:30-10:45AM

CoffeeBreakCoordinatedBacterialBehaviorsSessionConvener:DavidGlanvilleAlginate-associatedproteinsinPseudomonasaeruginosabiofilmsHollyM.JacobsUniversityofWashington,Seattle,WA,USA

10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:00PM12:15-1:30PM

Geneticdeterminantsofsyntehtic4-oxazolidinonesmallmoleculebiofilminhibitioninSalmonellaTyphimuriumKyleF.GriewischNorthCarolinaStateUniversity,Raleigh,NC,USANutrient-dependenteffectofOprFonPseudomonasaeruginosabiofilmformationBooShanTseng

UniversityofNevadaLasVegas,LasVegas,NV,USAIdentificationofthebindinginterfacebetweenthebiofilmregulatoryproteinsNspSandMbaAinVibriocholeraeErinC.Young

AppalachianStateUniversity,Boone,NC,USA

ExploringspatialdynamicsofantibiotictoleranceinthecysticfibrosislungSophieE.DarchUniversityofSouthFlorida,Tampa,FLUSAThedynamicsofbiofilmmatrixassemblyinthemodelorganismPseudomonasaeruginosaCourtneyReichhardtUniversityofWashington,Seattle,WA,USALunch(GreatRoomDiningRoom)

1:30-4:30PM4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-

FreeTimeHappyHourDinner(GreatRoomDiningRoom)

THEWELKER-STREIPSMEMORIALPLENARYADDRESS

Cyclicdinucleotides:FromcholeratocancerDr.ChristopherWaters

MichiganStateUniversityEastLansing,MI

SessionConvener:MartyRoop

7:00-8:30

Sunday,June9,2019

Breakfast(GreatRoomDiningRoom)Departure

Prokaryotic Biology - The Key to Understanding The Complexity of...

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