Musketeers Course 2012

In Course Presentation

Yogyakarta, May 2th, 2012

Tenri A.W.

MAJOR CONCEPT

“The BIG IDEA”

MAJOR CONCEPT

WHAT IS DENDRITIC CELL?

DENDRITIC CELL (DC)IS AN ANTIGEN PRESENTING CELL (APC)

DC IS EXPRESSING SEVERAL PATTERN-RECOGNITION RECEPTOR(PRR), INCLUDING TOLL-LIKE RECEPTOR (TLR)

MAJOR CONCEPT

ROLE DENDRITIC CELL IN IMMUNITY

DC IN TLR-MEDIATED ANTIGEN RECOGNITION

DC IN ACTIVATION AND MIGRATION

DC IN ANTIGEN PRESENTATION AND ADAPTIVE IMMUNITY ACTIVATION

ROLE DENDRITIC CELL IN IMMUNITY

DC IN TLR-MEDIATED ANTIGEN RECOGNITION

DC IN ACTIVATION AND MIGRATION

DC IN ANTIGEN PRESENTATION AND ADAPTIVE IMMUNITY ACTIVATION

TOLL-LIKE RECEPTOR

TLR, LIGAND, AND ADJUVANT

(DUIN ET AL., 2006)

ROLE DENDRITIC CELL IN IMMUNITY

DC IN TLR-MEDIATED ANTIGEN RECOGNITION

DC IN ACTIVATION AND MIGRATION

DC IN ANTIGEN PRESENTATION AND ADAPTIVE IMMUNITY ACTIVATION

TLR SIGNALING PATHWAY

TLR SIGNALING PATHWAY

SIGNALING MOLECULE

PHOSPORILATION CASCADE

TRANSCRIPTION FACTOR

ACTIVATION

CYTOKINE SECRETION

TLR SIGNALING PATHWAY (CONT)

TLR SIGNALING INDUCE CCR7 EXPRESSION

TLR SIGNALING UPREGULATE MHC II AND CO-STIMULATORY MOLECULE

ROLE DENDRITIC CELL IN IMMUNITY

DC IN TLR-MEDIATED ANTIGEN RECOGNITION

DC IN ACTIVATION AND MIGRATION

DC IN ANTIGEN PRESENTATION AND ADAPTIVE IMMUNITY ACTIVATION

DC PRESENT ANTIGEN TO NAÏVE T CELL

DC INDUCE BOTH CD 4 & CD 8T CELL ACTIVATION

DC INDUCE CD 4 T-CELL DIFFERENTIATION

IN MOST CASE, VIA TLRACTIVATION, INDUCE

DEVELOPING T HELPER 1 RESPONSE

(TRINCHIERY, 2003)

TH 1 CELL in INTRACELLULAR DEFENSE

TH 1 INDUCE Ig G, NK CELL, MACROPHAGE AND OTHER CELL ACTIVATION IN INTRACELLULARDEFENSE MECHANISM

MAJOR CONCEPT

VACCINE IN INFECTIOUS DISEASE

MYCOBACTERIUM BCG VACCINE

• BCG VACCINE IS USING TLR 2 AND TLR 4 – MYD88 PATHWAY

TLR 2 - TLR 4 – MYD88 PATHWAY

VACCINE IN MELANOMA CANCER

• MELANOMA CANCER VACCINE IS DNA VACCINE, USING TLR 9 – MYD88 PATHWAY

PRINCIPLE OF TLR SIGNALING

THANK YOU TO THESE GUYS

MAJOR CONCEPT

SUMMARY