Web-based analysis and annotation tools

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The Crown Human Genome Center & The National Lab for Genomic Infrastructure

Supported by the Ministry of Science

WIS Genome &Bioinformtics

Nili Avidan

WIS Genome & Bioinformtics

Use blast 2 sequence from BLAST tool to compare the new sequence to the known cDNA in the database

Use BLAST 2 sequence with blastX as program to detect AA change

AA sequence from database

Your new DNA sequence

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GESTALT figure of an analyzed genomic intervalGESTALT figure of an analyzed genomic interval

Blastn results of this genomic sequenceBlastn results of this genomic sequence

KIAA0001H963

H963 KIAA0001 K1LGR P2Y12

GPCR genes cluster(-strand)

aTRALPUSH(+strand) b

5’3’

5’ 3’

P2Y12LGR

NCBI clones

AC011103

AC078816

AC024886

AC020636

centromere telomere

76.9153. 0560.4175.00

54.07 62.34 55.60

Proscan promoter prediction

mRNA sequencesmRNA sequences• Full mRNA sequences: RefSeq

• Expressed Sequence Tags (ESTs): dbEST

• EST clusters: UniGene

• Predicted genes

• EST assemblies: – TIGR Human Gene Index – The HIB database of annotated UniGene clusters (MIPS) – AllGenes based on DoTS assemblies (U Penn.) – STACK (SANBI)

H89282H89282

MIPS assembly H11548S1 length: 1373Contain 21 members

TIGR assembly THC394205length: 1373Contain 12 members

*Predicted protein annotation

Multiple proteins alignment tool

Copy/past your protein and it ortholog/homolog here

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MULTICLUSTAL alignment of the five GPCRsMULTICLUSTAL alignment of the five GPCRs

Copy/Past you protein in the box

Found two known motif Histidine acid phosphatase at AA391-405 and a Zinc finger at AA1269-1292

Kidney, embryonic cell line

Normal prostate tissue

Brain, primary tumor

Kidney, embryonic cell line

Brain, normal, Post-mortem

Brain, medulloblastoma

Thank You!

Web-based analysis and annotation tools

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