Post on 18-Jan-2023
transcript
Differential staining
• Acceptance of stains is an important
property of bacteria and is the base
divission of bacteria to 2 principal groups
Gram positive and Gram negative in
taxonomy. • Gram staining
GRAM STAINING PROCEDURE • Prepare a heat fixed smear of the culture you
wish to examine
• Flood the smear with crystal violet (30 sec. to 2 min)
• Quickly and gently wash off excess stain (2 seconds)
• Flood the smear with Grams iodine (1 minute)
• Decolorize with alcohol (10-20 seconds or until the excess alcohol which flow off the slide is colorless)
• Quickly and gently wash off excess stain (2 seconds)
• Flood the smear with safranin (carbolfuchsin) (30 sec to 2 min.)
• Quickly and gently wash off excess stain (2 seconds)
• Blot dry with bibulous paper
• Examine your slide under the microscope. Record sketches of the organisms, size, color, morphology, and culture identification.
Gram stain
1. Staphylococcus epidermidis
2. Moraxella catarhalis
3. E.coli, C.albicans
4. Moraxella catarhalis, Streptococcus pneumoniae
5. B.cereus, E.coli
Praktické cvičenie č. 3
Téma : Farbenie podľa Grama
1. Zhotovte fixovaný preparát z kultúry S.epidermidis, zafarbite podľa
Grama, mikroskopujte.
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2. Zhotovte fixovaný preparát z kultúry Moraxella catarhalis, zafarbite
Gramom, mikroskopujte.
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3. Zhotovte zmiešaný preparát z kultúr E.coli, C.albicans, zafarbite
podľa Grama, mikroskopujte.
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4. Zhotovte zmiešaný preparát z kultúr M. catarhalis, S.pneumoniae,
zafarbite podľa Grama, mikroskopujte.
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5. Zhotovte zmiešaný preparát z kultúr B.cereus a E.coli zafarbite
podľa Grama, mikroskopujte.
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Praktické cvičenie č. 3
Téma : Farbenie podľa Grama
1. Zhotovte fixovaný preparát z kultúry S.epidermidis, zafarbite podľa Grama, mikroskopujte.
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2. Zhotovte fixovaný preparát z kultúry Moraxella catarhalis, zafarbite Gramom,
mikroskopujte.
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3. Zhotovte zmiešaný preparát z kultúr E.coli, C.albicans, zafarbite podľa Grama,
mikroskopujte.
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4. Zhotovte zmiešaný preparát z kultúr M. catarhalis, S.pneumoniae, zafarbite podľa Grama,
mikroskopujte.
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5. Zhotovte zmiešaný preparát z kultúr B.cereus a E.coli zafarbite podľa Grama,
mikroskopujte.
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