• To determine the

effect of the E401 and

B401 formulations on

broccoli seedlings

(Brassica oleracea).

• Set the quantity

variation of the effect

of E401 and B401

formulations on

broccoli seedlings (Brassica oleracea).

OBJECTIVES OF THE TEST

• The experimental

design was Complete

randomized (BCA)

with three (3)

treatments and 2 replications (blocks).

• Treatment description:

EXPERIMENTAL DESIGN

No Treatment mL o g/L

Water

1 E 401 1,0

2 B 401 0,28

5 CONTROL -

Overall length:

Using a millimeter scale the

total height of the seedlings was

evaluated. The evaluation was

conducted 35 days after

sowing.

Unit of measure cm

Root Length:

Unit of measure cm

Using a millimeter scale

seedling height was evaluated

from the neck. The evaluation

was conducted at 35 days after

sowing.

Green weight :

Biomass.

Unit of measure gram (g) The

evaluation was performed at 35

days after sowing.

VARIABLE FOR EVALUATION

CONTROL B401 E401

WEIGHT (gr) WEIGHT (gr) WEIGHT (gr)

0,3 2 1

0,4 2 1

0,4 3 1,5

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 3 2

0,5 4 2

0,5 4 2

0,5 4 3

0,5 4 3

0,6 4 3

0,6 4 3

0,6 4 3

0,6 4 3

0,6 4 3

0,7 4 3

0,7 4 3

0,7 4 3

0,7 4 3

0,7 4 3

1 5 3

1 5 5

RESULTS - WEIGHT VARIABLE

E 401

B 401

CONTROL

AVERAGE WEIGHT

CONTROL B401 E401

0,57 3,533 2,45

0

1

2

3

4

5

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Weig

ht

(gr)

Number of individuals analyzed

Green Weight

TESTIGO

B401

E401

CONTROL B401 E401

TOTAL

LENGTH

TOTAL

LENGTH

TOTAL

LENGTH

15 28 21

15 29 21

15 30 24

17 30 24

17 30 25

18 30 26

18 31 26

19 31 26

19 31 26

19 31 27

19 32 27

19 32 27

19 32 27

20 32 27

20 32 29

20 32 29

21 33 29

21 33 29

21 33 30

21 34 30

21 34 31

23 34 31

24 34 31

24 34 32

25 35 32

25 36 33

26 37 34

26 38 34

26 39 37

29 41 37

RESULTS - TOTAL LENGTH

B401 E401

Witness

CONTROL B401 E401

12,9 32,93 28,73

AVERAGE TOTAL LENGTH

0

5

10

15

20

25

30

35

40

45

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Tota

l Length

(R

oot

Ste

m L

eaves)

Number of individuals analyzed

Total Length

TESTIGO

B401

E401

RESULTS – ROOT LENGTH CONTROL B401 E401

ROOT

LENGTH

ROOT

LENGTH

ROOT

LENGTH

5 10 8

6 11 9

6 11 9

6 12 10

6 12 10

6 12 11

7 13 11

7 13 11

7 13 11

7 13 12

8 13 12

8 13 12

8 14 12

9 14 13

9 14 13

9 14 13

10 15 13

10 15 13

10 15 14

10 16 14

10 16 14

11 16 14

11 16 15

13 16 15

13 16 15

13 16 15

13 16 16

14 17 16

14 21 17

15 22 19

AVERAGE ROOT LENGTH

CONTROL B401 E401

9,367 14,5 12,9

0

5

10

15

20

25

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Root

Length

Number of individuals analyzed

Root Length

TESTIGO

B401

E401

• The following plant

variables were evaluated:

length (cm), root length of

the plant (cm) and weight

(g). The B401 formulation

showed greater length

and greater biomass

compared to the control

formulation and the E401

CONCLUSIONS

AVERAGE WEIGHT

CONTROL B401 E401

0,57 3,533 2,45

CONTROL B401 E401

12,9 32,93 28,73

AVERAGE TOTAL LENGTH

AVERAGE ROOT LENGTH

CONTROL B401 E401

9,367 14,5 12,9

• Compared with the

control treatment, the

inculcation of biological

formulations, generates a

positive, measurable

response in leaf growth

and root development of

seedlings of broccoli

(Brassica oleracea).

CONCLUSIONS

AVERAGE WEIGHT

CONTROL B401 E401

0,57 3,533 2,45

AVERAGE TOTAL LENGTH

AVERAGE ROOT LENGTH

CONTROL B401 E401

9,367 14,5 12,9

CONTROL B401 E401

12,9 32,93 28,73