APPL. MICROBIOL. lyzed with one membrane filter depended upon the treatment of the milk (i.e., 10 ml for raw or pasteurized; 1 ml for milk heated at 145 C for 8 see; 0.1 ml for autoclaved milk). Autoclaving destroyed th6 inhibitory quality of the milk and served as a control throughout. APC of survivors lose their validity whenever plates contain more than 0.01 ml of milk, whereas the membrane filter Statement of Ownership, Management and Circulation required by the Act of October 23, 1962; Section 4369, Title 39, United States Code. 1. Date of Filing: October 1, 1965. 2. Title of Publication: Applied Microbiology. 3. Frequency of Issue: Bi-monthly. 4. Location of known Office of Publication: Mount Royal and Guilford Aves., Baltimore, Md. 21202. 5. Location of the Headquarters or General Business Offices of Publisher: 115 Huron View Blvd., Ann Arbor, Michigan 48103. 6. Publisher: American Society for Microbiology, 115 Huron View Blvd., Ann Arbor, Michigan 48103. Editor: George M. Savage, 1210 Forest Drive, Kalamazoo, Mich. 49001. Managing Editor: Robert A. Day, 1235 Wines Drive, Ann Arbor, Mich. 48103. 7. Owners: (If owned by a corporation, its name and address must be stated and also immediately thereunder the names and addresses of stockholders owning or holding 1 per cent or more of total amount of stock. If not owned by a corporation, the names and addresses of the individual owners must be given. If owned by a partnership or other unincorporated firm, its name and address, as well as that of each individual, must be given.) American Society for Microbiology, 115 Huron View Blvd., Ann Arbor, Mich. 48103. 8. Known bondholders, mortgagees and other security holders owning or holding 1 per cent or more of total amount of bonds, mort- gages or other securities are: None. 9. Paragraphs 7 and 8 include, in cases where the stockholder or security holder appears upon the books of the company as trustee or in any other fiduciary relation, the name of the person or cor- technique permitted accurate analysis of the sur- vivors in up ta 10 ml of milk. The membrane filter technique permitted the measurement of spore populations previously obscured by inhibi- tors. This investigation was supported by Public Health Service grant EF-00378 from the National Institutes of Health. poration for whom such trustee is acting, also the statements in the two paragraphs show the affiant's full knowledge and belief as to the circumstances and conditions under which stockholders and security holders who do not appear upon the books of the company as trustees, hold stock and securities in a capacity other than that of a bona fide owner. Names and addresses of individ- uals who are stockholders of a corporation which itself is a stock- holder or holder of bonds, mortgages or other securities of the publishing corporation have been included in Paragraphs 7 and 8 when the interests of such individuals are equivalent to 1 per cent or more of the total amount of the stock or securities of the publishing corporation. (a)* 10. A. Total No. Copies Printed (Net Press Run) 5270 B. Paid Circulation 1. To term subscribers by mail, carrier delivery or by other means ........... 4507 2. Sales through agents, news dealers or otherwise none C. Free Distribution (including samples), by mail, carrier delivery or by other means .................................. 68 D. Total No. of Copies Distributed........ 4575 (b)t 5514 4873 none 87 4960 * Average number of copies for each issue during preceding 12 months. t Single issue nearest to filing date. I certify that the statements made by me above are correct and complete. (Signed) Robert A. Day, Managing Editor. 1044 NOTES
Transcript
APPL. MICROBIOL.
lyzed with one membrane filter depended uponthe treatment of the milk (i.e., 10 ml for raw or
pasteurized; 1 ml for milk heated at 145 C for8 see; 0.1 ml for autoclaved milk). Autoclavingdestroyed th6 inhibitory quality of the milk andserved as a control throughout. APC of survivorslose their validity whenever plates contain more
than 0.01 ml of milk, whereas the membrane filter
Statement of Ownership, Management and Circulation requiredby the Act of October 23, 1962; Section 4369, Title 39, UnitedStates Code.1. Date of Filing: October 1, 1965.2. Title of Publication: Applied Microbiology.3. Frequency of Issue: Bi-monthly.4. Location of known Office of Publication: Mount Royal and
Guilford Aves., Baltimore, Md. 21202.5. Location of the Headquarters or General Business Offices of
Publisher: 115 Huron View Blvd., Ann Arbor, Michigan 48103.6. Publisher: American Society for Microbiology, 115 Huron
View Blvd., Ann Arbor, Michigan 48103.Editor: George M. Savage, 1210 Forest Drive, Kalamazoo,
Mich. 49001.Managing Editor: Robert A. Day, 1235 Wines Drive, Ann
Arbor, Mich. 48103.7. Owners: (If owned by a corporation, its name and address
must be stated and also immediately thereunder the names andaddresses of stockholders owning or holding 1 per cent or more oftotal amount of stock. If not owned by a corporation, the namesand addresses of the individual owners must be given. If ownedby a partnership or other unincorporated firm, its name andaddress, as well as that of each individual, must be given.)American Society for Microbiology, 115 Huron View Blvd., AnnArbor, Mich. 48103.
8. Known bondholders, mortgagees and other security holdersowning or holding 1 per cent or more of total amount of bonds, mort-gages or other securities are: None.
9. Paragraphs 7 and 8 include, in cases where the stockholder orsecurity holder appears upon the books of the company as trusteeor in any other fiduciary relation, the name of the person or cor-
technique permitted accurate analysis of the sur-vivors in up ta 10 ml of milk. The membranefilter technique permitted the measurement ofspore populations previously obscured by inhibi-tors.
This investigation was supported by PublicHealth Service grant EF-00378 from the NationalInstitutes of Health.
poration for whom such trustee is acting, also the statements inthe two paragraphs show the affiant's full knowledge and beliefas to the circumstances and conditions under which stockholdersand security holders who do not appear upon the books of thecompany as trustees, hold stock and securities in a capacity otherthan that of a bona fide owner. Names and addresses of individ-uals who are stockholders of a corporation which itself is a stock-holder or holder of bonds, mortgages or other securities of thepublishing corporation have been included in Paragraphs 7 and8 when the interests of such individuals are equivalent to 1 per
cent or more of the total amount of the stock or securities of thepublishing corporation.
(a)*10. A. Total No. Copies Printed (Net Press
Run) 5270
B. Paid Circulation1. To term subscribers by mail, carrier
delivery or by other means........... 4507
2. Sales through agents, news dealers orotherwise none
C. Free Distribution (including samples),by mail, carrier delivery or by othermeans.................................. 68
D. Total No. of Copies Distributed........ 4575
(b)t5514
4873
none
874960
* Average number of copies for each issue during preceding12 months.
t Single issue nearest to filing date.I certify that the statements made by me above are correct and
complete. (Signed) Robert A. Day, Managing Editor.
1044 NOTES
APPLIED MICROBIOLOGY, Nov., 1965Copyright ( 1965 American Society for Microbiology
Vol. 13, No. 6Printed in U.S.A.
AUTHOR INDEXAdams, A. Paul, 762Adams, Ralph, 625Adye, J. C., 208Albury, M. N., 227Alexander, A. D., 595Ambekar, G. R., 713Ammann, Elizabeth C. B., 546Anderson, A. W., 1030Anderson, R. F., 393Anderson, Robert I., 552Anellis, A., 37, 397, 527Angelotti, Robert, 140, 352Appleman, Milo D., 289Arthur, Robert M., 125Avigad, G., 686Ayres, J. C., 358
Bailey, E. G., 109, 115Baldwin, R. L., 194Bankole, R. 0., 673Banwart, George J., 77, 650Barton, N. D., 15Bautista, M., 977Beargie, R. A., 279Becker, B., 236Benarde, Melvin A., 103, 776Bennett, E. 0., 570Bennett, F. W., 725Bennett, R. E., 738Bennett, Reginald W., 181Berberich, Norbert J., Jr., 614Berkeley, William H., 830Berkowitz, D., 37, 397Bhat, J. V., 257Bhatla, M. N., 345Bladel, B. 0., 281, 743Blasco, Richard J., 473Block, S. S., 5Bluhm, Leslie, 646Board, R. G., 358Bolles, Dorothy, 128Boniece, William S., 248Boyle, W. C., 218Bracken, E. C., 279Bram, B., 842Branch, Arnold, 469Browder, Henry P., 491Buckwalter, Frank H., 505Bullock, Graham L., 89Busta, F. F., 851, 858, 1043
Canada, James C., 788Cantoni, C., 631
Carlucci, A. F., 663Carroll, B. J., 502Casas-Campillo, C., 977Casida, L. E., Jr., 327Casman, Ezra P., 181Cassell, E. Alan, 293Chamberlain, Robert E., 232Charney, W., 515Cherry, William B., 605Chibata, Ichiro, 618, 638, 680Christensen, C. M., 653Christiansen, Lee N., 1023Clark, Carol W., 646Cliver, Dean O., 387, 417Considine, Judith M., 464Corlett, D. A., Jr., 808, 818Corstvet, R. E., 348Cowart, Glenda S., 605Cox, Claire B., 447Cox, W. A., 956Crabtree, K., 218Crisley, F. D., 140Cropp, C. Bruce, 212
Dack, G. M., 1014Daoust, Donald R., 478Davis, E. N., 267Davis, J. L., 99Dela Cruz, Amy S., 201DeLorenzo, S., 515Demain, Arnold L., 757Deutsch, David R., 1040Dhala, Salim, 432Dias, F. F., 257Dimmick, R. L., 846Dixon, Glen J., 579Doctor, V. M., 713Dunlop, Stuart G., 831Dyer, Denzel L., 833
Edmonds, Paul, 823Edwards, J. L., Jr., 851, 858Eklund, M. W., 985El-Bisi, Hamed M., 478Elliker, P. R., 1030Elliott, R. Paul, 73Ellison, D., 595El-Shazly, K., 62Eppley, Richard W., 833Erickson, R. C., 738
Farber, Lionel, 625Featherston, W. R., 650
1045
AUTHOR INDEX
Fellowes, 0. N., 496, 694, 1038Fields, Michael J., 81Foster, E. M., 1023Frank, Hilmer A., 201Friend, Milton, 128Frommhagen, Laurence H., 895
Gabrielson, Mary O., 967Gaffney, Peter E., 507Gailiunas, Peter, 872Galton, Mildred M., 81Gardner, Roberta A., 564Gaudy, A. F., Jr., 345Gerber, N. N., 935Gibbons, N. E., 288Godding, Rogene M., 10Goerner, Gordon L., 314Gordon, D. F., Jr., 537Gori, Gio B., 93, 909Goulet, N. R., 70Graikoski, J. T., 244Granstrom, Marvin L., 776Grecz, Nicholas, 37, 397, 527, 1014Greenberg, Richard A., 281, 743Grim, Catherine A., 457Groninger, H., 985
Hahon, Nicholas, 865Hall, H. H., 1000Hall, Herbert E., 352Halle, Sidney, 503Halvorson, H. Orin, 373Hamdy, M. K., 15Harmon, L. G., 335, 1010Harrington, Rube, Jr., 494Harrison, Edward F., 212, 491Harstad, J. Bruce, 899Heiniger, Patricia K., 73Held, H. R., 132Hendlin, David, 757Henis, Y., 437Herzog, H. L., 515Hess, George E., 781Highsmith, Anita K., 34Hill, L. R., 631Hoadley, A. W., 575Hollowell, C. A., 918Hood, Donald W., 886Horvath, Raymond, 103Howe, Jean M., 650Hoyt, H. H., 635Hsiung, G. D., 967Huber, D. A., 37Hung, P. P., 216Hungate, R. E., 62
landolo, John J., 646Imada, Yukio, 1
Irgens, Roar L., 373Ishikawa, Setsuko, 680Israel, Bernard M., 776Ito, Hiroshi, 680Iyengar, M. R. S., 749, 755
Jackson, Marion, 757Jackson, R. W., 1000Jacob, Theodore A., 757Jay, James M., 120Jennings, J. C., 322Jerome, Norge W., 157Jones, R. H., 725Junek, A. J., 322
Kadull, Paul J., 925Kajihiro, Edwin S., 720Kakimoto, Toshio, 638Kaneshiro, Tsuneo, 939Kaplan, Murray A., 505Karlson, Alfred G., 494Kato, Jyoji, 638Kaufman, D. D., 443Kaufmann, 0. W., 521Kearney, P. C., 443Keith, Elizabeth S., 308Kellogg, Douglas S., Jr., 171Kelly, L. E., 340Kempe, L. L., 244Kende, Meir, 1026Kilton, Roger M., 543Kluepfel, D., 600Knaysi, Georges, 500Kobayashi, Kaetsu, 1Koft, Bernard W., 103Koike, Hideo, 122Kono, Haruyoshi, 882Kopp, Rudolf, 950Kordan, H. A., 825Krabbenhoft, K. L., 762, 1030Krause, Frank P., 160Kushner, D. J., 288
Lagoda, A. A., 393Lamanna, Carl, 460Lancini, G. C., 600Landi, S., 132Lange, Willy, 160Lannon, James H., 505Leatherwood, J. M., 771Lechevalier, H. A., 236, 935Lechevalier, M. P., 236Lee, J. S., 808, 818Lerke, Peter, 625Levine, S. I., 70Litchfield, Carol D., 886Litsky, Warren, 106, 478Liu, 0. C., 70
1046 APPL. MICROBIOL.
AUTHOR INDEX
Loehr, Raymond C., 1005Loken, K. I., 635Long, Sterling K., 973Longeri, Luis, 167Lum, Norma A., 201Luthi, H. R., 511Lynch, Victoria H., 10, 546
McClung, L. S., 559McConnell, S. J., 595McCoy, Elizabeth, 218, 575McCune, R. W., 22McDaniel, L. E., 109, 115McDivitt, Maxine E., 157Majumdar, M. K., 190Majumdar, S. K., 190Mallette, M. F., 460, 464Malligo, John E., 931Margalith, P., 876Marks, C. L., 216Markus, Z., 686Marshall, Robert S., 521, 559Massey, Richard L., 798Masson, G., 288Mateles, R. I., 208Mattoni, Rudolf H. T., 798May, K. N., 340Mayberry, W. R., 698Meredith, W. E., 86Merrifield, Larry S., 660, 766Meseck, E., 515Midura, T. F., 244Miller, G., 686Miller, V. Richard, 77Milone, N. A., 244Mirocha, C. J., 653Misenheimer, T. J., 393Miyauchi, D., 985Morihara, Kazuyuki, 793Moyer, J. C., 511Mukherjee, S. K., 227Muller, Johannes, 950Munson, Polly S., 831Muraschi, Thelma F., 128Murawski, A., 515
Nagatsu, Chizuko, 669Nelson, G. H., 653Nelson, John D., 801Nutini, Leo G., 614
Olivieri, Vincent P., 776Olson, B. H., 314, 322Ordal, Z. John, 646
Packett, L. V., 22Palmquist, D. L., 194Parikh, G. C., 122
Parker, R. B., 1042Patel, K. L., 749Pathak, S. G., 262Patrick, Roger, 973Patrick, W. C., III, 99Payne, W. J., 698, 702Pederson, C. S., 227Peeler, J. T., 140Phillips, C. Alan, 457Phillips, James W., 460Pittman, Margaret, 447, 564Pivnick, Hilliard, 365Pollock, Marlene, 945Poonawalla, F. M., 749, 755Power, Edna E., 469Powers, John J., 308Pridham, Thomas G., 43, 1000Prochazka, G. J., 702Proteau, P. R., 838
Ray, John G., Jr., 297, 301, 305, 925Rhodes, R. A., 267, 272Richter, Alan, 503Rigg, J., 595Riley, H. D., Jr., 279Riley, W. H., 28, 945Robbins, Mary Louise, 1026Roga, V., 322Rohlich, G. A., 218Rose, Robert E., 106Rosemberg, J. A., 991Rosett, Theodore, 254Rosselet, J. P., 515
Sadler, W. W., 348Sano, Ryujiro, 618Santa Rosa, C. A., 81Sartori, G., 600Schabel, Frank M., Jr., 579Schipper, I. A., 762Schmidt, E. L., 673Schmitt, J. A., Jr., 290Schneider, M. D., 37Schneierson, S. Stanley, 286Schuurmans, D. M., 322Schwarz, Y., 876Schwegler, Diann T., 1005Seligmann, Edward B., Jr., 830Sellers, Sara M., 579Seshadri, R., 262Shaulis, Louis, 103Shay, Donald E., 297, 301, 305, 706Shechmeister, I. L., 122Shelton, Sharon, 801Shirato, Shiroh, 669Shore, Bella, 286Shotts, Emmett B., 34Shulke, H. Russell, 267, 272
VOL. 13, 1965 1047
AUTHOR INDEX
Siddique, I. H., 635Sidwell, Robert W., 579Siepmann, R., 827Silbernagel, S. B., 663Silvestri, L. G., 631Simon, M., 37Sinnhuber, R. O., 808, 818Smith, Rodney F., 706Smuckler, Stan A., 289Snyder, 0. P., 527Solomons, G. L., 847Speck, M. L., 537, 851, 858, 1043Spinelli, J., 985Stadelman, W.AJ., 650Starkey, D. H., 469Steenbergen, J. Frank, 559Steinkraus, K. H., 227Stoyla, Brigitta, 511Strong, Dorothy H., 788Stuart, Philip F., 365Stutzenberger, F. J., 570Sudman, M. S., 290Sulzer, Catherine R., 81Sundar Raj, C. V., 432
Taber, W. A., 590, 827Tagari, H., 437Takahashi, Joji, 1Takao, Shoichi, 732Tamir, Musha, 437Tardrew, P. L., 216Tertzakian, G., 590Thadani, S. B., 713Theriault, Robert J., 402Thomason, Berenice M., 605Tosa, Tetsuya, 618Toussaint, Andre J., 552Traxler, R. N., 838Traxler, R. W., 838Tyler, D. D., 393
Ueda, Seinosuka, 882
Vadehra, D. V., 335, 1010Valu, Joseph A., 486Van Veen, A. G., 227Vitali, Ronald A., 757Volcani, R., 437
Wagenaar, R. O., 1014Wahba, A. H., 291Walker, A. A., 527Wallace, D. L., 1010Wallen, L. L., 272Wang, Wen Lan L., 831Ward, B. Q., 502Wasserman, Aaron E., 175Wasserman, Felix E., 1040Weiser, H. H., 86Wessman, G. E., 426White, Lendell A., 171Wick, Warren E., 248Williams, Joy P., 698Winter, A. R., 86Wolf, Paul A., 28, 945Wolin, M. J., 918
Yager, R. H., 595Yamada, Koichi, 1Yamada, Shigeki, 680Yang, H. Y., 660, 766Yeatman, John, 387Yokoya, Fumio, 993York, George K., 993
Zemelman, Radll, 167Zimmerli, A., 109, 115Zingelmann, W. J., 743Zweig, Gunter, 939Zygmunt, Walter A., 491
305agar-gel precipitin-inhibition technique for plaque
antibody determinations, 925agglutination test for leptospiral antibodies, 81L-alanine, production by Pseudomonas dacunhae,
638algae, continuous culture of, 833algae, gas exchange of, 546algae, mass assays of physiological characteristics
of, 798algae, mass culture of, 473alpha sarcin, new antitumor agent, 314, 322amino acid, new, sarcinine, 314amino acid composition of cell-wall proteins, 650L-amino acids, production by bacteria, 618anaerobes, pipette count method for, 1042animal passage, effect on Clostridium perfringens,
788antibacterial heterocyclic quaternary ammonium
compounds, site of action of, 956antibiotic, new, from Streptomyces erythreus, 216antibiotic sensitivity of microorganisms, tube
dilution test for, 469antibiotic sensitivity of organisms from mammary
secretions, 762antibiotic sensitivity of staphylococci in milk, 725antibiotic susceptibility, determination of, 279antibiotics, effect on leptospires in tissue culture,
595antibiotics, new, cephaloglycin, 248antibiotics, new, cephaloridine, 248antibiotics from Micromonospora, 515antibiotics from Physarum gyrosum, 464antibodies, leptospiral, agglutination test for, 81antigens, viral, purification of, 895antimicrobial activity of bis-methanethiolsul-
fonates, 945antimicrobial activity of vitamin K5, 766antimicrobial effect of xanthines, 432antitumor agent, new, alpha sarcin, 314, 322
antiviral activity of 1,3-bis (2-chloroethyl)-1-nitrosourea, 579
Aspergillus flavus, aflatoxin production by, 208Aspergillus flavus, in soil, 673Aspergillus niger, enzyme production by, 842asphalt, effect of bacteria on, 838asphalt, fungistats in, 28
Bacillus, germination temperatures of, 500Bacillus lentimorbus, effect of antimicrobial agents
on, 1000Bacillus polymyxa, production of 2,3-butylene
glycol from citrus wastes, 973Bacillus popilliae, effect of antimicrobial agents
851, 858Bacillus subtilis spores, viability in rocket propel-
lants, 10bacon, radiation sterilization of, 37bacteria, L-amino acid production by, 618bacterial disintegration, 1010bactericidal activity of chlorine dioxide, 776bacteriophage, growth in Shigella flexneri, 831bacteriophage, streptococcal, destruction kinetics
of, 478bacteriophage aerosols, sampling of, 899bacteriophage detection, filtration method for, 1005bacteriophage 80/81 strains of Staphylococcus
aureus, action of lysostaphin against, 491bacteriophage 80/81 strains of Staphylococcus
aureus, action of penicillins against, 491bacteriophage types of staphylococci in milk, 725bacteriophage typing, determination of coagulase
activity during, 34bacteriophages in activated sludge, 257barbital metabolism by Streptomyces mediterranei,
600basidiomycetes, organic acid production by, 732bat guano, dermatophytes in, 720Bdellovibrio, in activated sludge, 257Betabacterium, deoxyribonucleic acid composition
of, 631bile salts, effect on microorganisms, 77biodegradability of organic compounds, 702biotin in seawater, 8861,3-bis(2-chloroethyl)-l-nitrosourea, in vivo anti-
of, 945brilliant green, effect on microorganisms, 77bruised tissue, Staphylococcus aureus in, 15
1049
1050 SUBJEC'
2,3-butylene glycol, production from citruswastes, 973
C-reactive protein, determination of, 297, 301, 305calcium gluconate, production by Penicillium
chrysogenum, 713Candida, method for identification of, 286carbohydrates, effect on protease production, 669carbohydrates, effect on streptomycin produc-
tion, 669carbon dioxide, effect on glucose catabolism, 507carob pod extract, effects in artificial rumen, 437casein hydrolysate medium for Pasteurella hemo-
lytica, 426catabolism, effect of carbon dioxide on, 507cell culture, rack for use in, 503cell disruption with a peristaltic pump, 460cellulase from rumen microorganisms, 771cell-wall preparation from actinomyctes, 236cell-wall proteins, amino acid composition of, 650cephaloglycin, laboratory evaluation of, 248cephaloridine, laboratory evaluation of, 248cheese, Clostridium botulinum in, 1014chicken meat, bacteria in, 340Chlorella pyrenoidosa, gas exchange in, 546Chlorella pyrenoidosa, heterotrophic growth of,
402Chlorella pyrenoidosa, mass culture of, 473Chlorella pyrenoidosa, xanthophyll production by,
bial degradation of, 443chloroform, sensitivity of viruses to, 1040chlortetracycline, in poultry tissues and eggs, 86chromatography, gas-liquid, for determination of
organic acids in fermentation media, 22citrus wastes, production of 2,3-butylene glycol
from, 973clostridia, method for isolation and enumeration
of, 281Clostridium botulinum, development in milk, 521Clostridium botulinum, growth in cured meats, 1023Clostridium botulinum, in processed cheese, 1014Clostridium botulinum, radiation killing of, 37Clostridium botulinum spores, in meat, 743Clostridium botulinum spores, radiation resistance
of, 527Clostridium botulinum spores, survival of, 397Clostridium botulinum type E, presence in water,
244Clostridium perfringens, technique for enumera-
tion of, 559coagulase activity of staphylococci, 34
jr INDEX APPL. MICROBIOL.
coffee cherries, decomposition in, 201color systems for actinomycetes, 43composts as media for mushroom production, 5computer analysis for identification of bacteria
in foods, 808, 818continuous cultivation of viruses, 909continuous culture of algae, 833corn steep liquor, effect on L-glutamic acid fer-
mentation by hydrocarbons, 1Corynebacterium, fermentative production of L-
glutamic acid by, 1coryneform bacterium, nucleotide production by,
757counting bacterial colonies, device for, 931counting data, method for estimating precision of,
Dactylium dendroides, production of D-galactoseoxidase by, 686
deoxyribonucleic acid, of Betabacterium, 631deoxyribonucleic acid, of Streptobacterium, 631dermatophytes, occurrence in bat guano, 720detergents, effect on Proteus, 950device for counting bacterial colonies, 931dialysis fermentor for mammalian cell culture, 93diet, effect on Staphylococcus aureus virulence in
mice, 614diquat, effect on Rhodospirillum rubrum, 939disintegration of bacterial cells, 1010
echovirus 7, purification of, 895egg yolk coloring material, production of, 876eggs, antibiotic residues in, 86eggs, bacterial infection of, 358endospores, thermal death rate, 993enterotoxin, detection in foods, 181enteroviruses, concentration and detection of, 387enteroviruses, membrane filtration of, 417enteroviruses, recognition by agar overlay
method, 967enzymes, activity in rumen microorganisms, 194epizootic, capsulated Pseudomonas from, 89Erwinia-like microorganisms, 128Escherichia coli, absence from rumen ecosystem,
918Escherichia coli, effect of oxygen supply rate on
growth of, 109, 115Escherichia coli, sensitivity to mercurials, 570estrogens, hydroxylation by Fusarium monili-
forme, 977everninomicin B, isolation from Micromonospora,
515everninomicin D, isolation from Micromonospora,
515
fecal specimens, detection of shigellae in, 605feces, isolation of salmonellae from, 365
fermentation of rumen microorganisms, 62fermentor, dialysis, mammalian cell culture in, 93fibrinogen-polymyxin medium for detection of
staphylococci, 157filtration method for bacteriophage detection, 1005fish, identification of bacteria in, 818fish muscle spoilage, bacteriology of, 625Flavobacterium species, properties of, 801flax, bacteria responsible for retting of, 991flor sherry, continuous production from wines, 511fluorescent cell-counting technique for virus assay,
865fluorescent-antibody assay for simian virus-40, 70fluorescent-antibody counterstain method for
detection of Neisseria gonorrhoeae, 171fluorescent-antibody technique, soluble antigen
in, 552fluorescent-antibody technique for observation of
soil fungi, 673food, Penicillium chrysogenum mycelium as, 262food-poisoning, enterotoxin in, 181foods, detection of staphylococci in, 140, 157foods, detection of viruses in, 387foods, rapid identification of bacteria in, 808, 818foot-and-mouth disease virus, detection of, 872foot-and-mouth disease virus, effect of surface-
active agents on, 694foot-and-mouth disease virus, storage stability of,
halophilic bacteria, simple method for killing, 288heat resistance of Clostridium perfringens, 788heat resistance of Salmonella, 73Herellea species, properties of, 801hydrocarbons, L-glutamic acid fermentation of, 1
idli, fermentation of the batter of, 227immunofluorescence, detection of shigellae by, 605incubator, temperature-gradient, improved, 73incubator for microbial growth, 846inosine-5'-monophosphate, production by coryne-
form bacterium, 757invertase production by Penicillium chrysogenum,
kinetics of, 478Lactobacillus fermenti, use of 4soleucine stereo-
isomers by, 680Lactobacillus leichmannii, survival in relation to
B12 assays, 486leavening action of lactic acid bacteria, 227leptospiral antibodies, detection in sera, 81leptospires in canine kidney tissue cultures, 595Leuconostoc mesenteroides, use as leavening agent,;
227lipases of Staphylococcus aureus, action on milk
fat, 335lymphocytic choriomeningitis virus, activity of
1,3-bis(2-chloroethyl)-1-nitrosourea against,579
VOL. 13, 1965 1051
SUBJECT INDEX
lysostaphin, activity against Staphylococcusaureus, 212, 491
lysostat, use in virus cultivation, 909
mammalian cells, concentrated cultures of, 93mammary secretions, antibiotic sensitivity of
organisms from, 762mass culture of Chlorella pyrenoidosa, 473meat, Clostridium botulinum spores in, 743meat, Clostridium perfringens in, 352meat, Micrococcus radiodurans in, 1030meats, effect of vacuum packaging on bacteria in,
1023meats, relation of water-holding capacity to
microbial quality, 120media for coagulase-positive staphylococci, 140medical microbiology, polarizing microscopy in,
543medium used in rapid enumeration of Clostridium
perfringens, 559mercurials, sensitivity of mixed cultures to, 570metabolism in Penicillium chrysogenum, 590microbial isomerization of amino acids, 618Micrococcus radiodurans, ecology of, 1030Micromonospora, chemistry of antibiotics from,
515microscopy, polarizing, in medical microbiology,
543milk, assay for neomycin in, 635milk, Bacillus stearothermophilus in, 1043milk, destruction of mycobacteria in, 494milk, development of Clostridium botulinum in, 521milk, staphylococci in, 157, 167, 725milk cultures, bitter peptide from Streptococcus
cremoris in, 537milk fat, action of Staphylococcus aureus
lipases on, 335milky disease bacteria, effect of antimicrobial
agents on, 1000Mima species, properties of, 801minerals, effect on neomycin production, 190mold growth in soils, 160molds, growth in liquid cultures, 827mushrooms, production on garbage compost, 5mutants, coryneform bacterium, nucleotide pro-
deficient, 663mycelium, Penicillium chrysogenum, use as food,
262mycobacteria, destruction in milk, 494Mycobacterium ranae, effect on asphalt, 838Mycobacterium tuberculosis, purified protein deriv-
ative from, 132
naringinase, production by Aspergillus niger, 842Neisseria gonorrhoeae, detection of, 171
neomycin, effect of minerals on production, 190neomycin in milk, assay of, 635Nocardia coeliaca, effect on asphalt, 838
organic acid production by basidiomycetes, 732organic compounds, biodegradability of, 702oscillator, sonic, cooling device for, 25410,10'-oxybisphenoxarsine, fungistatic action in
paints, 28oxygen, effect on aerosolized Serratia marcescens,
781oxygen consumption, device for recording, 125oxygen supply rates, effect on Escherichia coli
growth, 109, 115oxygen uptake, effect of carbon dioxide on, 507oxygen uptake by heterogeneous microbial popu-
lations, 345oxytetracycline, in poultry tissues and eggs, 86
paints, fungistats in, 28paper wastes, use for mushroom production, 5Pasteurella hemolytica, cultivation of, 426Pasteurella pestis, assay of antibodies for, 925Pasteurella tularensis, growth of vaccine strain of,
232pasteurization, destruction of mycobacteria in
milk by, 494penicillin acylase activity of Penicillium chryso-
genum, 738penicillins, activity against Staphylococcus aureus,
460pertussis vaccine, stability and potency of, 564pertussis vaccine testing, 447pesticides, microbial degradation of, 443phage, see bacteriophagephenolic acids, effect on bacteria in urine, 308photosynthetic growth of Rhodospirillum rubrum,
939Physarum gyrosum, antibiotics from, 464picornaviruses, comparison of elution and ad-
sorption, 457pigment production for differentiation of Tri-
chophyton species, 290pigments produced by Pseudomonas, 175
1052 APPL . MICROB3IOL .
SUBJECT INDEX
pipette ount method for obligate anaerobes, 1042plague antibody determinations, 925plant nutrients, removal from sludge, 373plant pathogen, isolation from animal hosts, 128polarizing microscopy in medical microbiology,
543poliovirus, titration in micro tissue culture, 1026poliovirus I, purification of, 895poly-j8-hydroxybutyrate, identity of sudanophilic
granules as, 218polymer formation by fleshy fungi, 267, 272polysaccharide produced by Pullularia, 882poultry, Salmonella infection of, 348poultry, Staphylococcus aureus in bruised tissue
of, 15poultry tissues, antibiotic residues in, 86,-propiolactone, use to inactivate contaminating
virus, 10383-propiolactone decontamination of simian virus-
40, 70proteases, extraction from bacteria, 1010proteases, production by Streptomyces griseus, 669protein, C-reactive, determination of, 297, 301, 305proteinase, production by Pseudomonas aeru-
ginosa, 793proteins, cell-wall, amino acid composition of, 650Proteus, effect of detergents on, 950protoplasts, steroid lysis of, 706pseudomonads in lake and stream waters, 575Pseudomonas, capsulated, from goldfish, 89Pseudomonas, degradation of organic compounds
by, 702Pseudomonas, pigments produced by, 175Pseudomonas, primary alcohol sulfatase in, 698Pseudomonas aeruginosa, proteinase production
Salmonella, heat resistance of, 73Salmonella, maximal growth temperature of, 73Salmonella infection of market poultry, 348salmonellae, effect of bile salts on, 77salmonellae, effect of brilliant green on, 77salmonellae, isolation by selective motility sys-
tem, 365sarcinine, new amino acid, 314seawater, microbiological assay for organic com-
pounds in, 886seawaters, effect on development of Serratia
marinorubra mutants, 663sera, leptospiral antibodies in, 81Serratia marcescens, effect of oxygen on, 781Serratia marcescens, growth in thermal gradient
incubator, 846Serratia marcescens, production of 2-ketogluconic
acid by, 393Serratia marinorubra, in assay for organic com-
pounds in seawater, 886Serratia marinorubra mutants, biochemically
deficient, 663serum, determination of C-reactive protein in,
297, 301, 305sewage, microbial populations in, 345sewage, removal of plant nutrients from, 373sewage, survival of Shigella in, 831sewage sludge, use for mushroom production, 5Shigella, survival in sewage, 831shigellae, detection by immunofluorescence, 605simian virus-40, ,-propiolactone decontamination
552sonic oscillator, cooling device for, 254spacecraft, sterility of propellants for, 10spoilage of fish muscle, bacteriology of, 625spoilage of meats, relation to water-holding capac-
ance of, 244staphylococcal enterotoxin, detection in foods,
181staphylococci, coagulase activity of, 34staphylococci, detection in milk, 157, 167staphylococci, media for detection of, 140staphylocci in milk, 725Staphylococcus aureus, action of lysostaphin
against, 491Staphylococcus aureus, action of penicillin against,
491Staphylococcus aureus, antibiotic action on, 212Staphylococcus aureus, factors affecting virulence
in mice, 614Staphylococcus aureus, fate in bruised tissue, 15Staphylococcus aureus, growth in cured meats,
1023Staphylococcus aureus, repression in associative
culture, 646Staphylococcus aureus, sensitivity to mercurials,
570Staphylococcus aureus lipases, action on milk fat,
335Staphylococcus Medium No. 110, improved, 289sterilization of bacon by radiation, 37steroids, in lysis of protoplasts, 706Streptobacterium, deoxyribonucleic acid composi-
by, 600streptomycetes, color of, 43streptomycin, production by Streptomyces griseus,
669submerged culture, calcium gluconate production
in, 713submerged culture, production of aflatoxins in,
208submerged fermentation, invertase production
in, 749sudanophilic granules of Zooglea ramigera, 218sulfatase, primary alcohol, in Pseudomonas, 698surface-active agents, effect on viruses, 694surface water bacteria, 575swine, effect of Fusarium-infected feed on, 653
Ti bacteriophage aerosols, sampling of, 899temperature, effect on bacterial infection of eggs,
358tetracycline, inactivation with sodium boro-
hydride, 505tetracycline, vitamin B12 assay in prescence of, 755thermal death rate of bacterial endospores, 993thermal destruction kinetics of streptococcal
bacteriophage, 478thermal gradient incubator for microbial growth,
846thermal inactivation of Bacillus subtilis spores,
851, 858thiamine, effect on L-glutamic acid fermentation
by hydrocarbons, 1tissue culture technique for virus titration, 1026tissue culture vessel for gas exchange, 825tissue cultures for virus detection, 872Trichophyton mentagrophytes, pigment production
by, 290Trichophyton rubrum, pigment production by, 290tube dilution test for antibiotic sensitivity of
microorganisms, 469tuberculin purified protein derivative, 132tularemia vaccine, live, 232
urine, effect of phenolic acids on bacteria in, 308uterus, effect of toxic substance from Fusarium
on, 653
vaccine, pertussis, testing of, 447vaccine, tularemia, live, 232vaccines, pertussis, stability and potency of, 564vacuum packaging of cured meats, effect on bac-
terial growth, 1023variola virus, assay of, 865
1054 APPL. MICROIBIOL.
SUBJECT INDEX
viral antigens, purification of, 895virus, cultivation in the lysostat, 909virus, foot-and-mouth disease, detection of, 872virus, lymphocytic choriomeningitis, activity of
nation of, 70virus, storage stability of, 496virus, variola, assay of, 865virus contamination of antisera, 1038viruses, comparison of elution and adsorption, 457viruses, detection in foods, 387viruses, effect of surface-active agents on, 694viruses, membrane filtration of, 417viruses, recognition by agar overlay method, 967viruses, sensitivity to chloroform, 1040vitamin B12 , assay in presence of tetracycline, 755vitamin B12 assays, survival of Lactobacillus leich-
mannii in relation to, 486
Month
JanuaryMarchMayJulySeptemberNovember
vitamin K5, antimicrobial activity of, 766vitamin K5 as a fungistatic agent, 660
waste water, microbial populations in, 345waste water, treatment with chlorine dioxide, 776waste water, Zooglea ramingera in, 218water, Micrococcus radiodurans in, 1030water, polluted, isolation of streptococci from, 106water, Pseudomonas in, 575water, treatment with chlorine dioxide, 776wines, production of flor sherry from, 511
xanthines, effect on bacteria, 432xanthophylls, production by Chlorella pyren-
oidosa, 402xanthosine-5'-monophosphate, production by
coryneform bacterium, 757
yeast, effect on egg yolk color, 876yeasts, from Pacific crab meat, 985
