14S Operator Manual

8/7/2019 14S Operator Manual

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SY-LAB Instruments GmbH. Austria Operator Manual - IceCube 14S

Operation Manual IceCube 14S Ver. 1.21b Rev 003 2007 SY-LAB Gerte GmbH.All Rights Reserved 2008-01-23

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Contents

Contents ___________________________________________________________________________ 2Warranty ___________________________________________________________________________ 5Purpose ___________________________________________________________________________ 6

Specification _______________________________________________________________________ 6

Freezer ________________________________________________________________________ 6Power Connection________________________________________________________________ 6Normal Conditions of Use __________________________________________________________ 7Transportation and Storage Conditions _______________________________________________ 7Additional Specification ____________________________________________________________ 7Safety Regulations and Symbols ____________________________________________________ 7Safety Notes ____________________________________________________________________ 8Periodic Inspections ______________________________________________________________ 8Liquid Nitrogen Dewar and Tubing ___________________________________________________ 8

Safety _____________________________________________________________________________ 9General Precautions ______________________________________________________ 9Special Precaution (Liquid Nitrogen, Tubing etc.) _____________________________ 10Precaution for Transportation ______________________________________________ 10Packing Contents ________________________________________________________ 11Fuses __________________________________________________________________ 12Important Specific Notes __________________________________________________ 13

Installation and Check-out ___________________________________________________________ 14Site Preparation, Space and Clearance ______________________________________________ 14Environment ___________________________________________________________________ 14

Unpacking ______________________________________________________________ 15IceCube Installation - Wiring and Hook-Up ___________________________________ 17

Variant A: _____________________________________________________________________ 17Variant B: _____________________________________________________________________ 17Monitor Assembly _______________________________________________________________ 19Variant C: _____________________________________________________________________ 20Variant D: _____________________________________________________________________ 21

Connecting the LIN Hose to Freezer and Supply Vessel ________________________ 22Pt-100 Transport Protection _______________________________________________ 22Description of the Instrument Components and Connections ___________________ 23

Turn ON/OFF and Check of the System______________________________________________ 24Handling of Temperature Sensors __________________________________________ 25Freezing Chamber _______________________________________________________ 27

Description ____________________________________________________________________ 27Available Freezing Racks _________________________________________________ 28

Program Log-In ____________________________________________________________________ 33Description of Program Tools and Buttons ___________________________________ 34

Menu Area ____________________________________________________________________ 36Main Menu - File ______________________________________________________________ 36

Edit description _____________________________________________________________ 36


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Open historical data _________________________________________________________ 39Process directory _________________________________________________________ 39Open last run ____________________________________________________________ 39Set source directory _______________________________________________________ 39Print Diagram, Print Program & Events, Print both _______________________________ 39Close window ____________________________________________________________ 39

List of Available Tools ____________________________________________________ 42

Show description, Show program, Display Total View ___________________________________ 42Create / Edit freezing program _________________________________________________ 43Set data directory ___________________________________________________________ 43Set program directory ________________________________________________________ 44Exit ______________________________________________________________________ 45

Main Menu - Display ___________________________________________________________ 46Total View_________________________________________________________________ 46 Tracking View ______________________________________________________________ 46Settings __________________________________________________________________ 46

Time axis _______________________________________________________________ 46Temperature axis _________________________________________________________ 47Time elapsed / remaining __________________________________________________ 47Actual program position ____________________________________________________ 47Temperature value to track _________________________________________________ 47LNi pressure _____________________________________________________________ 47

Main Menu - Options __________________________________________________________ 48Description of the various conditions and alarms __________________________________ 49

Start Temperature reached condition _________________________________________ 49LNi pressure Alarm (not implemented on 14S) __________________________________ 49Allowed Temperature deviation ______________________________________________ 50Temperature reading invalid ________________________________________________ 50Critical power fail - awaiting user assistance ____________________________________ 50Short time power fail ______________________________________________________ 50Freezing program finished __________________________________________________ 51

Save Settings ______________________________________________________________ 51User Administration _________________________________________________________ 52Info for the Administrator ___________________________________________________ 54Language _________________________________________________________________ 55

Main Menu - Version Info ________________________________________________________ 56Main Menu - Maintenance ________________________________________________________ 56

Ice-Water Calibration _________________________________________________________ 56Communication _____________________________________________________________ 57

Writing a Test Program ___________________________________________________ 58Test program for finding the crystallization temperature of a sample ________________________ 58

Freezing program editor ______________________________________________________ 59Edit control - Program _____________________________________________________ 59Edit Freezing program points ________________________________________________ 59Axis standard _____________________________________________________________ 62

Mouse button _____________________________________________________________ 62Active point size___________________________________________________________ 62Moving Screens and Zoom Functions____________________________________________ 62

Running the Test Program ________________________________________________________ 63Operation Panel ____________________________________________________________ 66

Seeding ________________________________________________________________ 66Hold ___________________________________________________________________ 66Next ___________________________________________________________________ 66Load program/Stop _______________________________________________________ 66

Writing the final program - Optimization (as an example) ______________________________ 68Special Function - Autoseeding ________________________________________________ 72

Setting the Autoseeding Level _______________________________________________ 72Setting the Restart Level ___________________________________________________ 76


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Seeding ___________________________________________________________________________ 78Auto(matic)Seeding ______________________________________________________ 78

Maintenance _______________________________________________________________________ 79Leak Check__________________________________________________________________ 79

Cleaning of the Freezing Chamber and Instrument ____________________________ 79Handling of Temperature Sensors________________________________________________ 79Cleaning____________________________________________________________________ 79

Settings on the PC __________________________________________________________________ 80Disabling of Start-Up Error Message ___________________________________________________ 80Freezer Software (Re-) Installation ____________________________________________________ 83Inserting Your Own LOGO ___________________________________________________________ 90Troubleshooting ___________________________________________________________________ 91Freezer and Accessories ____________________________________________________________ 96

Furniture _______________________________________________________________ 96Spare Parts 14S ____________________________________________________________________ 97Appendix _________________________________________________________________________ 98

Statement of Conformity (220 - 240 V AC) ____________________________________ 98Statement of Conformity (110 - 120 V AC) ____________________________________ 99Block Diagram Software ________________________________________________ 100

Instruction Manual Supplement ____________________________________________________ 101Autoseeding Unit for Welded High Security Straws (CBS) ____________________ 101

Introduction ___________________________________________________________________ 102New Seeding Techniques _____________________________________________________ 102Loading the CBS

Straws ______________________________________________________ 103Position of Autoseeding Unit in Freezing Chamber __________________________________ 105Part Numbers to Order ________________________________________________________ 106


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WarrantySY-LAB warrants for one year from the date of shipment each instrument manufactured to be free fromdefects in material and workmanship impairing its usefulness under normal use and service conditionsexcept noted herein. Our liability under this warranty is limited to repair, servicing and adjustment, free ofcharge at our plant, of any instrument or defective parts, when returned prepaid to us, and which our

examination discloses to have been defective. The purchaser is responsible for all transportation chargesinvolving the shipment of materials for warranty repairs. Failure of any instrument or product due tooperator error, improper installation, unauthorized repair or alteration, failure of utilities, or environmentalcontamination will not constitute a warranty claim. The materials of construction used in SY-LABinstruments and other products were chosen after extensive testing and experience for their reliability anddurability. However, these materials cannot be totally guaranteed against wear and/or decomposition bychemical action (corrosion) as a result of normal use.Repair parts are warranted to be free from defects in material and workmanship for 90 days from the dateof shipment.No instrument shall be returned to us without our prior notice (return authorization no. required).SY-LAB shall be released from all obligations under its warranty in the event persons other than its ownauthorized service personnel make repairs or modifications unless such work is authorized in writing bySY-LAB.

The obligations of this warranty will be limited under the following conditions:Certain products sold by SY-LAB are the products of reputable manufacturers, sold under their respectivebrand names or trade names. We, therefore, make no express or implied warranty as to such products.We shall use our best efforts to obtain from the manufacturer, in accordance with his customary practice,the repair or replacement of such of his products that may prove defective in workmanship or materials.If an instrument or product is found defective during the warranty period, replacement parts may, at thediscretion of SY-LAB, be sent to the purchaser for installation, e.g. printed circuit boards, magneticvalves, temperature sensors, etc.Expendable items (e.g. o-rings, seal-rings, indicator lamps, fuses, etc.) are excluded from this warrantyexcept for manufacturing defects. Such items, which perform satisfactorily during the first 45 days afterthe date of shipment, are assumed to be free of manufacturing defects.Due to the sensitivity of the temperature sensors (Pt-100), the warranty period on these devices will onlybe 12 weeks. This applies also to parts made out of PMMA (acrylic glass).

Damage due to any static discharge. Before handling the temperature sensors, we advice the operator todischarge himself by touching shortly the grounded metal wall of the freezing chamber.Damage due to lightning, over voltage and/or heavy voltage/current fluctuation on the supply line.

Freezer: 1 yearPC 1 yearLCD Monitor: 1 yearPlease Note: For warranty purposes, it is necessary to mention model and serial number of PC and LCDMonitor on YOUR invoice to the end user.Do not return any items to SY-LAB without having a RAN (return authorization number).Note: Shipping- and insurance costs are not covered by SY-LABPlease read the warranty instructions accompanying the PC and monitor.In most countries, PC and monitor can be repaired locally.
http://dict.leo.org/?p=2Ib6..&search=acrylichttp://dict.leo.org/?p=2Ib6..&search=glasshttp://dict.leo.org/?p=2Ib6..&search=glasshttp://dict.leo.org/?p=2Ib6..&search=acrylic


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PurposeCompact, computer controlled freezing system with integrated, network-compatible PC and connection toliquid (LIN) pressure vessel up to 22 psia.

This instrument will be used mainly in the field of cryo -preservation of organic materials. With the help ofsuch instrument, it will be possible to freeze and thaw organic matter under controlled temperatureconditions. This in turn can lead to a higher survival rate (viability) of the material in question afterprolonged periods of storage at cryogenic temperatures. As a cooling media, Liquid Nitrogen at atemperature of -196C (-321F) will be used.

Specification

Freezer

Temperature Range: +40 to -180 Celsius (+104 to -292 Fahrenheit)Manual and automatic control of heating and coolingPt-100 Temperature measurement with 2 (standard) and/or. 3 or 4 optional resistance

thermometers using 4 wire technique, 1.5 mm , 65 mm standard length or 100 mm (optional).Electrical power-turn-OFF to motor, fan, heater and magnetic valves when lid is opened.Build-in safety valve opens at approx. ( 50 psi ) 3,4 barVariable LIN conn. from either side of the freezer with original tubingMonitoring and alarm messaging of supply pressure (optional)Maximum recommended working pressure: 1.5 bar (recomm. 0,5-0,8 bar)

22 psi (recomm. 7 to 12 psi).Autoseeding upgrade possible

Note: Freezer can not be operated above 22 psi - error message shows up on screen whenoptional pressure monitoring system is installed.

Cooling rates: 0.01-60C/minHeating rates: 0.01-20 C/min (in range -180C to -50C)0.01-15 C/min (in range - 50C to +20C)

Display accuracy: 0.01CHeating power: 750W

Note:The above heating and cooling rates were measured with the freezing chamber empty.

Power ConnectionFreezer: (has to be specified on order)

220-240VAC (100-120VAC), 50/60 Hz, 800VA

Minimum PC Config.Windows 2000 or XPP, 1x serial, 1x USB, CD-Rom drive and 3 1/2" Floppy disk recommended.Freezing Chamber(LxWxH in mm): 217 x 217 x 348(LxWxH) in inch): 8,5 x 8,5 x 13,7, 16,4 Liter (~4,3 gal.) chamber volume,Stainless Steel, hinged transparent glass cover, prepared for AutoSeeding, additional sample sensorspossible, thermo fuse over heat protection, optional external alarm outputs.Dimensions Freezer (LxWxH in mm): 540 x 420 x 520 (w/ PC system)

(LxWxH in inch): 21,3 x 16,5 x 20,5 (w/ PC systemHeight with TFT monitor: 740 mm (~29,2 in.)Height with opened lid: 820 mm (~32,3 in.)Weight approx.: 29 kg (~63 lbs.)Required area (LxWxH in mm): 850 x 550 x 870 (depending on configuration)

(LxWxH in inch): 33,5 x 21,6 x 34,3 ( -----------------------------------)


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Dimension Trolley (LxWxH in mm): 710 x 565 x 720(LxWxH in inch): 28 x 22,3 x 28,4

Capacity Options1080 (0, 25 / 0, 5 ml) straws or 480 (2ml) vials or 240 (5 ml) vials, 11 metal cassettes or several bloodbags (number varies according on bag size and fill volume)

Software, Data OutputUnlimited number of programs, 12.1 Touch Monitor (Variant B-D), program menu driven via TouchMonitor, removal of the heat of crystallization due to counter cooling, Hold and Next Ramp function,free adjustable start temperature, thermo sensor calibration routine, zoom function, Date- and Timeintegrated filename, color inkjet printer (option), program-, chamber- and sample curve.

Normal Conditions of UseVoltage: (has to be specified upon ordering)

220 - 240 VAC (100-120 VAC), 800 VA(without monitor, printer and PC)

Frequency 50/60 Hz +/- 2%

Ambient Temp. Range: 15-35CHumidity : 20 to 90% relative humidity (at 15-35C)Position: Flat and sturdy surface

Transportation and Storage ConditionsIf the following mentioned conditions are met, correct operation of the instrument can be expected.

Temperature: +5 to +40CHumidity: 10 to 90% relative humidity (non-condensing)

After long term storage, an ice-water calibration might become necessary.

Additional SpecificationProtection against memory loss after power failure.Built in Lithium batteries keeps configuration parameters from being lost during power failure. Thenominal lifetime of such batteries is expected to be around 10 years.

Safety Regulations and SymbolsTerms and definitions used on the instrument and in this handbook.

General WarningsWarnings signalize the danger of instrument damage, personal injury or even loss of life.

Notes: Notes signalize addition precaution or attention.

Caution, read accompanying instructions

Manufacturer. Mentions name and address of manufacturer.

Date of Manufacture

Serial number of instrument

CE marking of instrumentAC Alternating CurrentDo not dispose with household waste

LIN - Liquid Nitrogen


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Safety NotesThis instrument complies to safety class I test voltage 1kV (with protective ground).

This instrument was manufactured under following current norms in regard to electromagnetic

compatibility (EMC) for companies in residential, commercial and light industrial areas.EN 61000-6-1:2001EN 61000-6-3:2001+A1:2004

Upon request, EMC test reports are available for viewing.

PCs and peripheral equipment such as monitors, printers, etc. must comply to the norm

EN 60950-1

During operation, maintenance or any other repair work, the following special safety instructionshave to be followed.

If the warnings and mentioned safety precautions in this instruction is not followed, safe operationcannot be guaranteed.SY-LAB will not be responsible for any damage or harm violating aboves statement.

Periodic InspectionsSafety-related tests according to VE/NORM E 8751-1 or VE/NORM E 8701-1 or IEC 62353 orcorresponding Norms have to be performed periodically. The test interval must not exceed 3 years.The tests have to be performed by skilled personnel. For further information on this, please contactSY-LAB Austria or its representative.

Liquid Nitrogen Dewar and TubingLiquid Nitrogen supply vessel must comply with the local regulations for pressure vessels. Working

pressure of 0, 3 to max. 1, 5 bar (7 to 22 psi), volume of 60 to 240 liters depending on the number offreezing cycles. Larger vessels require probably longer supply tubing (available through SY-LAB).For supply tubes longer than 1.3 m, new cooling and heating parameters might have to be acquired.

Caution: Liquid Nitrogen supply tubes acquired at SY-LAB are made of StainlessSteel. These tubes remain flexible even at very low temperatures such as LiquidNitrogen. If these tubes come with insulation (for protection against burns), theinsulation material will harden at low temperatures and eventually stiffens. In thiscondition, the tube / insulation assembly must not be moved at all, otherwise, theinsulation will crack. Just keep in mind that this does not induce any dangeroussituation since the inner part of the insulation, the metal tubing, will stay flexible andwill not crack.

To avoid cracking of the insulation part, let the system warm up to ambienttemperature (takes about 15 to 30 minutes).

Note: To keep the instrument at its highest technical standard, SY-LAB reserves theright to make modifications / change specifications without the prior notice of thecustomer.


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Safety

General PrecautionsFor your personal safety, read thoroughly the following sections before turning theinstrument on for the first time.Pay attention to all notes, safety and warning messages mentioned in this manual andshown on the instrument.Always disconnect the instrument from the power line before attempting to open, clean orperform any service work. Do not use abrasives or cleaning sprays except when it isasked for in the manual. Use only a wet soft cloth for cleaning.Place the instrument on a flat and stable surface (table or desk).The openings on the freezer housing are mainly used for cooling purposes. Do not blockthese openings otherwise, the instrument might get damaged due to overheating. Also,do not place the instrument close to heat sources or ventilation systems such asradiators, fans, etc.Before connecting the instrument to the mains, make sure that the voltage rating shown

on the type-plate agrees with your main supply. If in doubt, contact your SY-LAB GmbH.representative or SY-LAB GmbH. Austria directly.Use only the original power supply cable. A grounded (ground potential) wall socket isrequired. If the wall socket does not fit the instruments supply cable, contact your localelectrician.The instrument must be connected to a socket with functioning protective earthconductorThe electrical environment of the instrument has to comply to the local safety regulations.In Austria, this is VE-EN 1 or VE/NORM E 8001 (construction of electrical systemswith nominal voltage up to 1000V AC)When using extension wires or distribution boxes, be sure not to exceed the maximumpower rating of all instruments connected. This is normally about 15A AC.Never try to insert objects into any instrument openings. Electrical shock might be the

result, which in turn might be harmful to life. Do not pour any liquid into any of theopenings.With the exception of work asked for in this manual, never try to repair theinstrument by yourself. Only authorized personnel must perform repair work.For important sample material, we always recommend using an UninterruptiblePower Supply (UPS).

In all following cases, disconnect the instrument from the mains and call your servicerepresentative:

Power supply cable or connector worn or defectiveLiquid was spilled and entered the instrument

Instrument shows malfunction when used according to the operator manual.Note: Use only accessories (as mentioned in the operator manual) intended to be usedwith this type of instrument.

Instrument was dropped and/or housing is damaged.Instrument shows strange behavior as compared to normal operation.When instrument was stored for a long time.

Note: For any service requests, always mention the instruments model-, serial- and thesoftware version number.


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Special Precaution (Liquid Nitrogen, Tubing etc.)Liquid Nitrogen (LIN) is dangerous when it comes in contact with skin or when it gets into youreyes. Use special care when handling LIN. For safety reasons, you must wear protective gloves,goggles and a leather apron.

The new model freezers are built with metallic seals and must not be used with any additionalseal rings. If the LIN Connector is damaged, it must be replaced.During a freezing cycle, the LIN supply tubing is getting very cold and if necessary, must only betouched with protective gloves. Ignoring this warning might result in severe burns and/orfrostbites.Areas exposed to excess nitrogen gas (storage tanks, freezers etc.) must be well ventilated.Under normal circumstances, keeping a window area permanently open (at upper half of theroom) will be inevitable. Nitrogen gas is not poisonous but it will displace the oxygen in the air.For safety reasons, the use of an oxygen monitor system (available through SY-LAB GmbH.) isrecommended. For badly ventilated areas, it will be mandatory. In any case, please contact yoursafety department for local regulations.National norms have to be considered.A safety instruction booklet will accompany all supply Dewars delivered by SY-LAB. This bookletmust be read and be understood before handling any liquid nitrogen equipment.Also, for safety reasons disconnect the instrument from the power line whenever maintenancework is performed on the instrument. Do not open the instrument while it is connected to thepower-line. Electrical shock might result. We will void all warranty and will free ourselves from anyobligations if our instruments are opened without the prior written consent of the manufacturer.

Precaution for TransportationDuring the transportation of the freezer, it is absolutely necessary to secure the Pt-100 temperaturesensors. Use some Styrofoam or similar pad and secure the sensors with tape to the inside wall of thefreezing chamber. This keeps the sensors from bouncing around during transportation.

Caution: For normal operation of the freezer, the Pt-100 transport protection must beremoved. Not doing so will lead to abnormal temperature oscillations.

In addition, do not place any foreign parts or chamber furniture into the freezing compartment.Damage might occur if done so and will void any warranty.In case of printer or monitor failure, file a report to SY-LAB GmbH. and contact your local printer /monitor representative. Most printer / monitor manufactures have worldwide warranty service. Useonly the original packing or pack separately when returned with the freezer. No return shipments willbe accepted by SY-LAB without a Return Authorization Number (RAN). Do not throw the packingmaterial away. Keep it for possible shipments in the future.


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Packing ContentsPlease check the contents of the delivered goods. Obvious damage to packing or instrument mustimmediately be reported to the shipping agent. Any damage must be recorded on the delivery papers(invoice, etc.). If the shipment is accepted, then only with "reservation".

In general, if no additional items were ordered, depending on the Variant, it should consist of thefollowing:

Variant A - Free standing freezing chamber - PC system provided by thecustomer

IceCube freezer - basic unitPower cableSerial communication cableOperator ManualIceCube Freezer Software (CD)Hardlock (key)

Necessary area for freezing chamber + PC system (L x W in mm): 540 x 400 (without PC)(L x W in inch): 21,3 x 15,7 (-----------)

Variant B - Free standing freezing chamber with Touch Monitor

IceCube freezer - basic unitPower cableSerial communication cableTouch Monitor package

Monitor mountOperator ManualIceCube Freezer Software (CD)Hardlock (key)

Necessary area for freezing chamber + PC system (L x W in mm): 740 x 400(L x W in inch): 29 x 15,7

Variant C - Freezing chamber with Touch Monitor and standard PC-mount

IceCube freezer - basic unitPower cable

Serial communication cableTouch Monitor packageMonitor mountStandard PC mount (two arms) with strapsOperator ManualIceCube Freezer Software (CD)Hardlock (key)

Necessary area for freezing chamber + PC system (L x W in mm): 830 x 400(L x W in inch): 32,7 x 15,7

Note: Max. PC - height: 390 mm (15,4 in.)


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Variant D - Freezing chamber with Touch Monitor and mini PC-mount

IceCube freezer - basic unitPower cableSerial communication cableTouch Monitor packageMonitor mountMini PC mount (single arm)Operator ManualIceCube Freezer Software (CD)Hardlock (key)

Necessary area for freezing chamber + PC system (L x W in mm): 740 x 400(L x W in inch): 29 x 15,7

Note: Max. PC - height: 300 mm (11,8 in.)

Optional items for above Variants:

Computer system originally packed

PrinterAccessory package - supply tubing, wrenches, etc.Furniture f. freezing chamber - racks, seeding, spacer, etc.Customized orders possible

Note: For monitor, printer and PC package contents, please consult the accompanying operatormanual of the corresponding manufacturer.

FusesTwo Fuses protect the IceCube against overload. Use only original fuses. Thefuses are located next to the main - power switch of the Freezer Electronic Box.

Value: 2x T 5 A L / 250VType: 20 x 5 mm fuse

Caution: Disconnect the freezers power supply cable from the mains. Neverchange a fuse or perform internal work with the power cable plugged into themains supply.

Fuses


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Important Specific NotesComputer System from third party suppliers must comply with the territorial regulations.The IceCube in the standard configuration comes with two temperature sensors. One chamber-and one sample sensor. Optional, two more sensors can be added. However, this cannot be

done in the field. So either you order the IceCube already with additional sensors or the freezerselectronic box is sent to SY-LAB for up-grade.Liquid Nitrogen Vessels should only be filled to about 90% of their nominal filling volume. The freespace above the liquid level is needed for gas building assuring continues supply of Nitrogen tothe IceCube.Computer systems not purchased at SY-LAB, the operating system, the freezer software as wellas the Touch Monitor software must be installed. The instructions for loading the freezer programinto your PC are found in this manual. The installation of the Touch Monitor is described in themonitor handbook.Since there will be no keyboard connected to your PC, deactivate "Hold on All Errors" in thecomputer BIOS. If this is not done, your PC system will stop when looking for the keyboard.Use only the original Liquid Nitrogen supply tubing from SY-LAB. The original supply tubingallows the placement of a supply vessel at either side of the IceCube freezer.For supply vessels up to 70-liter content, the supply tube should be 1 meter long. For largervessels, a 1,3 meter supply tube should be sufficient. If other length is required, pls. contact SY-LAB.Tubing Connections: 3/4" UNF Nut to G 1/4" conical. Both ends use metallic sealing. No sealrings are required.Lock the brakes on the supply vessels wheels in order to avoid unintended movement.Due to the very low temperature on the LIN supply tubing and connections, water condensationwill occur. This is seen as white fog dropping to the floor. Water droplets in the surrounding aircondense out and will sink to the ground. This is a normal process and has nothing to do with aleak in the LIN system. Nitrogen gas is invisible and odorless.Never disconnect a cold supply tube from the supply vessel or the IceCube freezer. Waituntil the supply tube has reached ambient temperature. If moisture due to condensation

gets into the supply tube, it will lead to blockage of ice or to freeze the magnetic valve.For normal operation of the freezer, the Pt-100s transport protection must be removed. Not doingso will lead to heavy temperature oscillations.3 power sockets are required for operation (4 if printer is also used).The IceCube Freezer must be connected to a computer that complies to EU-norm EN 60950-1(Information technology Safety)


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Installation and Check-out

Site Preparation, Space and ClearanceAn opening is provided on the backside of the freezer. It is used as an exhaust for the

nitrogen gas. Position the IceCube in such a way that the cold exhaust gas cannotdamage any other equipment nearby. Keep at least a distance of 15 - 20 cm (6-8inches) to other equipment. A 90 elbow pipe is available optionally to divert theexhaust (see under Accessories).

Additional requirements:

Position the IceCube on a flat, horizontal and stable surfaceThe instrument must be accessible from all sidesDo not place the instrument such that it is exposed to direct sunlight, extensive heat or cold,humidity and dust.Do not use extension wires when connecting the instrument to the power line. These connectionsbecome weak with time and will lead to malfunction of the equipment.

You must be able to remove the power cord from the wall socket at any time. Do not block theaccess to the power socket.Do not use any wall sockets that can be turned ON or OFF by an extra switch or timer. Besidesloosing data when automatically turned off, the sample material might be ruined as well.Do not use wall sockets where other heavy instruments such as motors, copy machines, ovens,etc. are connected. Such devices can shortly deplete the supply voltage when turned on.The computer system and its components should not be placed near big speaker systems,cordless- and mobile docking stations, etc.Freezer, computer and all other system components must be connected to the same powersource having the same grounding potential. Potential differences due to bad grounding arepotential sources of error and can lead to the destruction of any connected items. No warrantywill be granted in such cases.

EnvironmentThe instrument will work without any difficulties under normal working conditions (15-35 C / 60-95 F).Nevertheless, in a humid environment, excessive condensation on the LIN-supply-tubing will occur and isnormal. The environment should be such that 90% humidity (non condensing) is not exceeded. Very highhumidity can lead to internal condensation and destruction of the instrument. Also, during transportation,it is vital that the instrument is given time to adjust to the surrounding temperatures before turned on,especially in the wintertime, when instruments are transferred from a cold to a warm environment or viceversa.Instruments on a trolley must be secured against movement. Use the roller-lock on the wheels to do so.


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UnpackingPrior to the shipping, the instrument was carefully inspected and packed. Because of the size and weight,it needs special packing. Unpacking instructions are attached to the outside of the box.Check for any transport damages before opening the shipping box. If any damage is visible, immediately

contact your shipping / insurance agent or better do not accept the shipment. If in doubt, make at least anote of the damage on the shipping papers. Take a picture as well.Check the contents of the package for completeness. Compare with the packing list, delivery note, etc.If any items are missing, please contact immediately either your representative or SY-LAB Austria. Laterreclamations will not be accepted.

Always state Model Number and Serial Number ofFreezer, Monitor, Printer, and so on.Do not return any items to SY-LAB without having aRAN (return authorization number).

Note: Shipping- and insurance costs are not coveredby SY-LAB.

Do not dispose the packing material. You might need itfor future shipments.

On the left, you see an original 14S shipping box.

Size of box (LxWxH in mm): 670 x 520 x 580(LxWxH in inch): 26,4 x 20,5 x 22,8

Open the box carefully from the top. Below the top, youwill find another cardboard with handles.

Carefully pull the instrument out and pay attention that the instrument does not slip out on one ofthe sides.


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Place the freezer on an appropriate location (see "Site Preparation and Clearance").

Depending on the Variant and options of your system, an accessory package will also be included (see"Packing Contents").


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IceCube Installation - Wiringand Hook-UpPlease consider the above mentioned

environmental conditions. Place the IceCube ona flat and horizontal surface. Consider thestability of your table, laboratory trolley, etc. TheIceCube weighs around 30 kg (~64 lb.).

Note: Only trained personnel should perform theinstallation of the IceCube.

Unpack monitor, printer, PC, etc. and inspectvisually for any damage. The monitor stand wasmodified at SY-LAB (Variant B-D) and does notexactly look like the one shown in the monitorhandbook.

In the following section, we will go step-by-stepthrough the instruments installation and hook-upprocedure.

Variant A:

(Free standing freezing chamber - PC systemprovided by the customer)

In this configuration, only the freezing chamberis available. For the system to work, a PCsystem including monitor and keyboard arerequired. For hard copies, a printer will be

useful.

The chamber comes with a power- andcommunication cable. Communication will beestablished between the freezers serialconnection and a free COM Port on your PC.Use the supplied power cable and attach it to awall outlet. Note: Make sure the freezers powerrating corresponds to your local supply (seeType-Plate).

Thereafter, the IceCube Software can beinstalled (see " IceCube Series SoftwareInstallation).

Variant B:

(Free standing freezing chamber and PC withTouch Monitor)

This type of configuration includes already theTouch Monitor.

During normal operation, no extra keyboard ormouse will be needed. For hard copies, a printerwill be useful.

In addition to the connections mentioned inVariant A, attach the stereo jack of the PC's line-out of the sound card to the line-input of themonitor (not on all units available). Also, attachthe monitors Touch-Screen USB-cable to one ofthe USB ports of your PC. As already mentionedabove, after a successful installation of theoperating system and the IceCube program incl.Touch Ware, etc., the keyboard and mouse willnot be needed anymore.


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(View from underneath)

Generally, the freezer series 14S will come in abasic configuration (without Monitor and PCsupport). Depending on the various types(Variant A to D), additional accessories will beincluded and must be assembled during theinstallation at site.In this configuration, a monitor base will beincluded.

Installation of the monitor base (Variant B)Remove the left upper side panel (face plate) asshown.

Attach the monitor base plate to the freezerframe (4 screws) as shown below. Replace theleft side panel (face plate).

Thereafter, slide the monitor base onto the studfor the monitor support as shown.

Turn the monitor base such that the locking

screw becomes visible. To secure the monitorbase, use a 3 mm hex driver (included in theaccessory package) and turn the locking screwcounter clockwise (outward).

With the monitor base secured, slide the TouchMonitor (if already assembled) onto it as shown.

VGAAnschlus

NetzteilAnschlus

TouchScreen


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The viewing angle can be set by adjusting theswivel head. The electrical wiring is describedfurther above.

Monitor AssemblyThe next step will be to modify the LCD Touchmonitor to fit onto the monitor holder

All necessary parts are shown below. Also, allrequired tools are part of this package.

Monitor holder 2 is already part of the IceCubeVariant B shipment.

First, mount the swivel on the mounting plate.

Then, attach the mounting plate to the back ofthe LCD monitor using the provided screws.

The viewing angle can be adjusted with thescrew on the swivel head.

And finally, the monitor assly. can be placed onthe monitor holder.

The electrical wiring was discussed furtherabove.

That completes the hardware installation.Now proceed to the installation procedure of thesoftware.

Note: If the IceCube freezer was orderedfrom SY-LAB together with a PC system,the software is already installed andfunctional.

MountingPlate

MonitorHolder 2

MonitorHolder 1

Swivel


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Variant C:

In addition to the configuration in Variant B, thismodel comes with a PC support (2 pieces).Instead of the small monitor base plate with only

one stud as in Variant B, this base plate issomewhat larger and is equipped with three (3)studs. The upper stud is for the monitor and thetwo lower ones for the PC support.

Replace the left side panel (face plate) andattach the monitor base as well as the PCsupports in the same way as described abovefor the monitor base.

Slide the Touch Monitor onto the monitor baseand place the PC onto the support as shown(See section above about Monitor Assly).

The electrical wiring is exactly the same asdescribed in Variant B


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Variant D:

The installation of the base plate remainsexactly the same as described in Variant B.

Thereafter, replace the left side panel (faceplate).

The accessory package of this version comeswith a single support for Touch Monitor andMini-PC. Attach the support as described earlier.

Place the Touch Monitor and the PC onto thesupport (See section above about MonitorAssly).

The electrical wiring is exactly the same asdescribed in Variant B.

After the successful installation of the hardware,the software must be installed onto the PC.

Please refer to the section on "Settings on thePC" and "Freezer Software Installation" furtherdown in this manual.

If the PC system was ordered from SY-LABtogether with the freezer, the software is alreadyinstalled and ready to be used.


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Connecting the LIN Hose toFreezer and Supply VesselThe optional accessory package includes theflexible stainless steel hose and a set ofwrenches. The conical metallic seal of the

supply hose does not require any additionalseals.

Attach the 1/4" conical end of the hose (smallconnector) to the freezer and the other end (3/4"UNF) to the supply vessel.

Caution: Do not over tighten the nuts. Justtighten the nuts slightly with thewrenches. Re-tighten the nuts after a fewminutes of a test run (cooling).

Use only original supply tubes from SY-LAB.Those tubes have a 90 bent on one side. Thisgives the user the possibility to place a supplydewar on either side of the freezer.

At this point, we again point out theimportance of wearing protectiveclothing. All personnel working with theIceCube must be instructed on how to handleliquefied gases at sub-ambient temperatures.

Read the safety instructions about handling LIN.Note: After a freezing run, do not immediatelyclose the liquid valve of the supply vessel. Leaveit open for another five minutes. Any LiquidNitrogen still in the supply hose will boil off andgenerate high pressure. If the liquid valve on thesupply vessel is open, excess Nitrogen pressurecan expand back into the vessel. Nevertheless,a safety valve is built into the IceCube. Anypressure in excess of about 3.5 bar (51 psi) willbe released to atmosphere.

When used in conjunction with an IceCube, themaximum working pressure of the supply vesselmust not exceed 1.5 bar (22psi). At higherpressures, the operation of the IceCube'sinternal magnetic valve cannot be guaranteed.

Pt-100 Transport ProtectionDuring transportation, the Pt-100 Sensorsare secured in a block of Styrofoam orsimilar material. This keeps the sensorsfrom bouncing around. The sensors are verysensitive and should be handled with care.Never bend or drop any sensors.Do not throw away the Styrofoam block. Youmight need it for future shipments.

LIN InputMax. P: 150kPa(1,5 bar)

Liquid Nitrogen Conn.Metallic Seal


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Description of theInstrument Components

and ConnectionsBefore the first turn ON of the freezer, re-checkall connections.In the following, we will describe eachcomponent in more detail.

These are:Freezer Electronic / BoxTouch Monitor (not on Version A)Printer (optional)PC-System

Freezer Electronic / Box

On the Freezer Electronic / Box, you will find themain switch, power connector, fuses, the Pt-100temperature sensor inputs and the COM serialport (9 - pin Sub D)

The main fuses of the freezer can be found onthe freezer electronic (electronic box / power

supply). Next to the fuses, you will find the mainpower switch and the power supply connector aswell as the temperature sensor inputs, the serialCOM connection and optional (potential free)alarm outputs.For replacing the main fuses, a small Philips

type screwdriver will be needed. The specifictype of Fuse can be found in the sectionFuses.

Caution: Before exchanging fuses orperforming any internal work, alwaysdisconnect the instrument from thepower line (mains).

There are 2 more openings for Pt-100 Sensorsprovided. In the standard configuration, the 2

spare holes are fitted with blind plugs. TheIceCube can be ordered with up to four sensors.A later up-grade from two to more sensors canonly be done at SY-LAB.

Via the COM Port, communication is establishedwith the PC. The communication cable canbe found in the IceCube accessories. Theconnections have to be secured with theprovided screws.

There are three connections on the TouchMonitor (view from underneath):

Power SupplyVGA signal andTouch Monitor USB

VGAconnectio

Powersupply

TouchScreen

PowerConnector

Fuses

MainSwitch

Pt-100Sensors

SerialCOM

Groundingwire


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Establish all connections. Power supply topower distribution box, VGA and USB TouchScreen to the corresponding inputs on the PC.

For getting printed results, reports, graphs, etc.,

a printer is recommended. It can be connectedto either the parallel (Centronics) port or to oneof the USB ports.

Now, all connection to PC and power distributionbox should be established.Depending on the type of PC you are using,there might be a switched power-output on thePC.The monitor normally uses this switched power-

out connector. We would like to point out, that

for the operation with the IceCube, thisconnector must not be used.

Turn ON/OFF and Check of theSystemThe freezer system consists of various modulesor components. It is therefore important to followa certain turn-on and off procedure. Turn on inthis order:

Freezer electronicMonitor

Printer (if available)PC

Important: For synchronization purposes,the monitor must be turned ON(especially for TFT Monitors) before thePC is switched on.

Upon turn-off, it is important to shutdown thecomputer system in an ordinary fashion;otherwise, possible data loss will be the result.Also, you might have trouble booting up your

system the next time. Please consult your PCmanual for proper Shut-Off.

After the PC has been shut-off, all othercomponents can be turned off.

Please note: The PC system needs some timeto boot-up. Generally, the boot-up should nottake longer than about one minute. Dependingon the settings, the IceCube can be startedautomatically when entered into the AutoStartlist or started manually by clicking onto theIceCube icon.

StorageAfter every freezing cycle, the freezing chambershould be warmed up to about ambient

temperature. This can be accomplished by juststarting a program, which has its starttemperature at about 20C. During the heat-up,keep the chamber closed. This has theadvantage that very little condensation will occurinside the chamber.Once warmed up, dry the chamber and the lidwith soft cloth or a sponge. If necessary, cleansethe system with a proper disinfectant.

Caution: Before cleaning the freezingcompartment, disconnect the IceCubefrom the power line (mains).

Store the IceCube in a dry and dust freeenvironment. Do not store below +5and above+ 40 Celsius. Do not exceed 90% humidity(non-condensing).

Do not use


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Handling of TemperatureSensorsThere are typically two temperature sensors in

the freezing chamber. The sensor feed troughsare color labeled.

The red sensor is the chamber sensor, the blueone is the sample sensor. When standing infront of the IceCube, the sensor closest to theoperator is the sample sensor. At all times, thesensors must be kept free of moisture. Thesample sensor (blue) is used for monitoring thetemperature inside a sample. IceCubes with

additional sensors are available. It can be of anadvantage to measure the temperature ofsamples at different points, e.g. in large bags.The temperature sensors are very thin, allowingus to insert them into 0.25 ml straws. In addition,the low mass of the sensor will give us a fastreaction time.When used in a vial, never try to force a sensorinto a vial cap. It is necessary to pre-drill orpunch (with awl, nail or small screwdriver) a littleopening into cap. Prepare the opening such thatthe sensor can be pushed easily through thehole.

Also, never try to pull out a sensor from a frozensample. Damage will be the result.To remove a sensor from the wall-holder, pullthe sensor cable slightly out of the feed through.The sensor can now easily be removed.

Caution: The chamber sensor (red) must neverbe inserted into a sample. This sensor controlsthe temperature in the chamber. If this sensor isobstructed, the chamber temperature cannotproperly be controlled.

Various different rack-systems are available

from SY-LAB. Choose the one most suitable for

you (e.g. vial- or straw racks, racks for homo-grafts, bone marrow or blood bags, etc.). If yoursystem is equipped with an auto-seeding option,various racks for vials and straws are availabletoo.

If only small quantities of samples are frozen,the freezing chamber is nearly empty; a spacercan be used for proper positioning of yoursamples closer to the lid. Since the samples arelocated closer to the top of the chamber, it willbe easier to visually check the samples (due tothe see-through chamber lid) during the freezingprocess, especially during auto-seeding.

As already mentioned, the blue sample sensorcan be inserted into a sample (straw, vial, bag,etc.). The red chamber sensor can either remainin the wall socket or be positioned in a well-

ventilated position near the sample material.

Note: The chamber sensor (red) should not bepositioned in between sample materials. Theheat released during the crystallization processof the sample influences the temperaturereading and might alter the temperature in thechamber.

Once, a program is optimized and working well,it will not be necessary, to continue inserting thesample sensor into a sample, especially if thesample quantity is limited.However, if enough sample material is available(for record keeping purposes), it will beadvisable to monitor the freezing process forevery freezing run performedNaturally, the sample used for monitoring must

not be used on a patient. It must be discarded.


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In many instances, if the concentration of asample in a cryo protectant solution is small, thecryo protectant by itself (a dummy) can beused for record keeping. Just remember, thesample sensor will show the temperature withinthe sample during the freezing process. In other

words, you know what is going on inside thesample.

For larger freezing bags, sensors with a lengthof up to 200 mm are available (standard lengthis 65 mm)

Once more please note:

For normal operation of the freezer, removethe transport protection from the Pt-100sensors.


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Freezing Chamber

Description

The IceCube Series Freezer is equipped with atransparent glass cover (lid). In this way, it ispossible to visually monitor the sample materialduring the program run. This is especiallyimportant when determining the point ofcrystallization during an Autoseeding run.

Another feature is the hinge. The glass covercan be opened without looking for extra deskspace. Due to the weight of the lid, the chamberis firmly sealed towards the top of the freezingchamber. An opening is provided at thechambers backside for expelling the gaseousNitrogen. As seen from the front, there are twotemperature sensors (standard configuration)positioned on the left side of the chamber wall.

Straight ahead towards the right side, a plug isprovided for the connection of the AutoSeedingrack. It is available as an option and can beinstalled at SY-LAB upon ordering or be installedin the field later on.AutoSeeding Option Kit # (14S): 13-159532

On the right side wall are the in- and outlets forthe air-stream. Nitrogen gas mixed with air willbe withdrawn at the top of the chamber, sweepsover the heater element (750W) to mix with theincoming Nitrogen to be expelled at the bottomof the chamber. So the flow of the air stream isfrom the top to the bottom.

The turbulence is generated by a fast movingfan, dispersing any incoming Liquid Nitrogen togaseous Nitrogen.

At very low chamber temperatures, some liquidnitrogen droplets are allowed to enter thefreezing chamber but will evaporate quickly off.


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Available Freezing RacksVertical Autoseeding rack for either 2 or 5 mlsize vials.

A maximum of 28 vials can be placed on onerack. One side of the tubing connection isinserted into the AutoSeeding outlet at thebackside of the freezing compartment. The otherend of the tubing is guided through the largeopening of the chamber and leads to the outsideof the chamber.

Caution: Make sure there are no delicateInstruments behind the Freezer. The extremely

cold gas expelled at the rear exhaust mightdamage them.

AutoSeeding rack for straws. It is available for0.25, 0.5, 1.0 and 5.0 ml straws. Additional strawholders are available.

Vial rack. It is available for 1.8, 2 ml or for 5 mlvials. Up to 5 racks can be placed on top of eachother for 2 ml vials and up to 3 racks for 5 mlvials each holding up to 96 vials.

Thus, a maximum of 480, 2 ml vials or 240, 5 mlvials can be placed in an IceCube 14S chamber.

Note: The freezing racks shown in this sectionundergo constant refinement and might not lookthe same in the future.

A typical temperature sensor positioning in anvial is shown. The tip of the sensor should beinserted to about half of the liquid level.

It is important that the tip of the sensor is not

touching the sidewalls of the containment(Vial). Keep the sensor centered. This can beaccomplished with a centering disk made ofplastic or similar material.


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A rack for the vertical freezing of blood bagsis shown below. This type of rack is adjustableand can be used for a variety of bags.Size of profile: 184 x 100 mm (7,3 x 4 in.)

It comes standard with 7 profiles and allows theplacement of 6 bags horizontally (depending onthe size of the bag). If smaller bags are used, ahigher number of bags is possible.

Vertical rack for metal cassettes. Up to 11cassettes can be place on such a rack.

For special applications, we have the possibilityto produce customized racks as well.

The next picture shows such a non standardrack

Rack for 20 ml glass bottles.

AutoSeeding rack for bags.

Manual Seeding for Straws

(Symbol photo)

The manual seeding system consists of anintermediate lid comprising 31 openings for the


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straw holder. Each straw holder can accept upto 4 straws.

Also, each straw holder can individually bepulled out of the freezing chamber for manual

seeding. Thus, the temperature in the freezingchamber remains fairly stable.The straws can be expelled from the strawholder easily by just turning the black knob atthe upper end of the holder.

Need a special rack for your samples? Pleaseask for a quote. Better, send us a sample of yourcontainment (bag, vial, glass, beaker, straw,

etc.).


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In the following example, we will show astandard vial rack resting on a spacer. Thissimulates a real time situation. The samplesensor is positioned in one of the containments,here in a vial. The chamber sensor remains inthe sensor holder. Depending on the application,

the chamber sensor can be positioned at anyplace within the chamber. In many cases, thechamber sensor is placed next to the samplematerial to react as close to the sampletemperature conditions as possible. It isimportant that the chamber sensor is nottouching any sample material, vials, freezingracks, etc. It should measure the temperature ofthe nearby airflow.

Various sizes are shown. The vial rack can be

used for 2ml, 4 or 5 ml vials as well as for manyother vials.

Please consult our accessories list at the end ofthis manual for more info on chamber furniture.

One of our furniture is the straws rack. Becauseof the amount of straws that can be positionedinto the IceCube14S chamber (up to 1620,

0.25ml or 1080, 0.5ml), the handling of straws

normally becomes difficult. In order to positionthe straws quickly onto the straw rack, we havemade a device, called "Straws DistributionBlock".

The Distribution Block is placed below the strawrack. Then, the straws are placed onto the rack.Because of the slits in the Distribution Block, thestraws will be equally spaced. Use a small rulerto straighten out the horizontal position of thestraws as shown below.

Once, the straws are positioned, the straw rackcan be lifted off and be stacked up to amaximum of 30 racks / chamber on the 14S,each holding up to 36 straws.


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The illustration below shows 3 empty racksstacked together.

New in our program is the Autoseeding Rack forWelded High Security (CBS) Straws

New seeding techniques have made itnecessary to re-design the current Autoseedingsystem. Previous systems used straws that were

sealed at one end with either powder material,tampons or with sealing pins (balls, etc.).Nowadays, many applications require straws tobe welded on both sides to preventcontamination (CBS High Security Straws). Instructions are provided in the Appendix.

Here, we show you some of the availablefreezing racks, ramps, autoseeding accessories,etc.


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Program Log-InNote 1: The surface of the Touch Monitors screen must only be touchedwith the soft tips of your fingersor with a soft stylus made out of plastic (such as the ones used on handheld PC's). Sharp and roughedges can lead to damage of the screen surface. Scratched or damaged surfaces are not covered by SY-LAB's instrument warranty. Before operating the Touch Monitor, it is mandatory to read the manufacturers

instructions and warranty terms. ---------------------------------------------Note 2: Initially, the IceCube Series program does not knowwhich of the Freezers (15M/L-14S) is connected to the PC.Only after the initial start of the IceCube Series software, thesubsystem of the particular freezer reports back to theSoftware and will "mention" its name.

--------------------------------------------When the IceCube program is first started, you will be asked to enter a User Name and a Password. Forfirst-time user, the User name and Password will be in both cases

sylab

Use the provided On-Line-Keyboard to enter YOUR User Name and Password if already assigned.

If you do not have this information, ask your IceCube Administrator for it. The Administrator has the rightsto sign you up as a new user. When using the above first-time User Name and Password, theadministrator is advised to change the default User Nameand Passwordfrom sylab to a real User Nameand Password(this procedure will be described further down).

Here, we enter, (first time users will enter sylab in both fields consult the section on User Administration to set up a new real

user)

E.g. Garner Jeffand Password

Press OK to get into the IceCube program or QUIT to revert backto the desktop.If you answered OK, a new screen will be presented to you like theone shown below.


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Description of Program Tools and ButtonsThe IceCube program includes several graphical tools. In addition, Windows based commandstructures are available as well. In the following section, we will describe the terminology of the various

buttons, display fields, and so on.

Main Menu Pop-up menu. Behind this menu button, sub menus are found for File, DisplayOptions,Settings, Version informationand Maintenance.

Please Note: The access to various pop-up menus depends on the USER-LEVEL granted to you bythe IceCube Administrator.Here, we assume that all levels (administrator level or when initially logged in with sylab - sylab) aregranted.

In the Filemenu, patient information, freezing programs, freezing runs and reports can be generated oredited as well as the data and program path (directory) be set.In Display Options, one can choose the way the program curve will be displayed. In Total View, thewhole temperature range +40C to -180C can be displayed. Tracking view follows the programtemperature and shows a somewhat narrower temperature range.

In Settings, the parameters for X-axis (time) and Y-axis (Temp.) can be defined, remaining or elapsedtime be shown on the screen, the current program temperature be displayed next to the temperaturecurve and the pressure curve be displayed. A more detailed description can be found further down in thismanual.In Options, some sub menus pop up. In Device settings, various alarms can be turned on/off and thepressure alarm limits be set. Userscan be administered, giving each user his own Name and Password.Finally, the language can be selected. At the moment, only German and English language files areavailable. More language files will be available in the future.Version Info gives us some information on the installed PC-program version as well as the versionnumber of the instruments resident program. In case of failure, this information together with theinstrument model and serial number should be given to the service personnel.Maintenance allows the user to perform an Icewater calibration as well as to set the correctCommunicationport

Display Area

Main Menu

InstrumentPanel

Logo Area

OperationPanel

Status Area


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Status Area Display of the current instrument action like heating or cooling ON or OFF, alarm 1 / 2status, ventilation system running, online- (CTS) and text status, etc. All statuses are displayed in form ofsignal lights. Is the light ON, is the corresponding function active.Display Area Graphical display of program-, chamber-, sample temperatures, and so on. Duringthe program run, the temperature curves are displayed.Instrument Panel Numerical display of program-, chamber- and sample temperature(s) and remaining

time or run time of a program. The display for the 3rd and 4th sensor is only active if these options areinstalled.Operation Panel Buttons for starting and stopping a program run, initialization and triggering ofmanual seeding, Hold and Continue button, Program Load button.toStartTempbrings the freezing chamber to the pre programmed start temperature. Seeding (option)opens an additional magnetic valve, Hold keeps the freezer suspended at the current status, Nextadvances the program run to the beginning of the next step and Stopstops the freezing cycle.Logo Area Above the non-removable SY-LAB logo, it is possible to add an additional logo, i.e.your own, if desired. A bitmap (.bmp) format with a size of 106 x 122 pixels should be used.

In the following, we will describe in detail the various tools and buttons.


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Menu Area

Main Menu - File

Under the Main Menu - File, six sub menus areshown as seen on the left.The Edit description will allow us to enter sample orpatient specific information.Open historical data allowsus to locate, view and tosome extent modify already finished freezing processdata. On the Validation Software package, allmodifications are recorded and are traceableIn the Freezing program, new programs can bewritten or existing programs be edited.The next two entries regarding data and programdirectoriesgive you the possibility to select your ownlocation (directories) where data and programs might(will) be saved.

Exitwill close the IceCube program.

Edit description

As already mentioned, on this screen, information about a sample or patient can be entered.The first field will accommodate the Freezing Program Name. You will be able to reach this screen eitherby selecting Edit description just described above or it will pop-up just before the actual program run isstarted.


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If the Edit description was selected before loading a program, no Freezing program name will bedisplayed. The program name will be automatically entered as soon as you load a program later on.However, information about a sample, patient, operator, etc. can be entered in the following field lines.The Lastfield will ask you for a sample or patient name.This field will be divided into a Last and First name area. It is important to enter some specificidentification in the Lastfield. This will help you later on in finding a specific sample or patient in your data

file directory.So Last will require some input. We would suggest entering the last (family) name of a patient or thename of a specific sample. The first 8 characters of Last will be part of an automatically generatedProcess record file name. The remaining characters of the name will be truncated. All other field entriesexcept the operator name are not required but are provided to enter other useful information such asSample or Patient ID and Memos. The Operator name will automatically be filled in and will be identical tothe Login name of the currently logged in user. If more than one user is operating this instrument, theymust each have a separate User Name. Pls. consult your system administrator to set you up as a newuser.On the bottom of the Edit descriptionscreen, the automatically generated process file namecan be seen.As you can see, the first part consists of the Lastname. In addition to the Lastname it will include theyear, month, day, hour, minute and seconds. In this way, there can only be one specific file name foreach freezing process. As an example, the file name might look like this:

Entering any information into the various fields is accomplished with the pop-up keyboard.

Garner_2003-03-18_13-47-26.prc

This corresponds to the file name convention

LAST_yyyy-mm-dd_hh-mm-ss.prc

The extension of the file name PRC indicates that it is a freezing PRoCess file.

As already mentioned earlier, this screen will also be presented toyou when you press the "to Start Temp" button after loading a

program. Keep in mind that in order to save a freezing processrecord, we have to give it a specific name. Therefore, you cannotstart an actual freezing run before giving it a Lastname. You MUSTenter something in the Lastfield.Also, use for the Last name only characters which are allowed

under the Windows file name convention. These are all characters A-Z, a-z, 0-9. Do not use specialcharacters such as / \ < > , . ,, etc.

Two Memo fields are available for adding additional information. These two fields can be edited evenafter a process file run whereas all other fields are protected against any changes. Just keep in mind,after a freezing run, the operator, patient name, ID, etc. must not be changed for safety reasons.However, some additional information, e.g. about the freezing run might need to be recorded (in theMemo field).

Note:In the validation version, any changes in the Memo fields are traceable and can not be deleted.

On the right of each memo field, a message will let the operator know of howmany changes were made to this particular memo field. If modifications weremade, "Show" will list all changes that were made in the past. It will show whothe operator was and at which time and date the "editing" occurred.

In our example, it will look like below. Zero "0" modification(s) has changed toone (1) showing that one modification was done to the particular memo field.


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Please keep in mind that the Show x modifications buttons are not present in thestandard IceCube Software.

As soon as the requested information is entered, you must press OK


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Open historical data

For viewing freezing process records, we have to get into the Open historical datamenu. Either a clearfreezing process screen is displayed or a previously loaded process record is shown.

On the right bottom of the screen, the Menubutton gets you into various sub menus.At this point, Show descriptionwill display the patient or sample information like in Edit descriptionabove.Showprogramwill display the original freezing program if one was loaded with Open process recordand

Display Total View will show both, theprogram curve as well as the associatedfreezing run.

In the Filemenu, you can choose among

Process directory, Open last run,Set Sourcedirectory, Print Diagram, Print Program &Events, Print both of above and Closewindow.

Process directory

When Process directory is selected,you first have to select a process recordfrom a file list, assuming that you havemade a freezing run previously. If not,this list will be empty and no historicaldata exists.

Open last run

Open last run will display the last freezing run that was made.

Set source directory

Set source directory allows you to choose the location where you want to store the Process record files.

Print Diagram, Print Program & Events, Print both

Print Diagram, Print Program & Eventsand Print both of above will produce a printout of the processcurve, process curve and a tabular listing of events ( Hold, Continue, Next, etc.) or both of the previous.Before printing starts, a preview will be shown on the screen.

Close window

Close window will allow you to exit this page and to revert back to the previous screen.


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Let us go back to the Process record for a more detailed discussion.

If this menu was chosen and a process file opened (e.g. Testcurv_2004-07-....), following screen will bedisplayed.

The process record will then be displayed, can be enlarged, etc. and be printed out.

The above curve is a typical freezing process test curve to

find the temperature of crystallization. However, here we cansee clearly the program temperature curve (green), sampletemperature curve (blue) and hidden behind the green curvethe chamber temperature curve (red).The area of interest can now be zoomed in (to enlarge thedisplay area, draw a rectangle around it from left upper toright lower corner) and be Added tothe Liston the right. Youcan choose as many windows as you like. When added tothe list, the window can be printed out later on.

In the right area field, you will see the windows information.Hier, 23.96 -4.13 44.52 -30.00

It means that the left upper corner ofthe selected window is at 23.96minutes and -4.13 C. Whereas theright lower corner of the window is at44.52 minutes and -30.00C.Choosing "Events" on the above screen, additional Information about each"Action" performed during the program run can be displayed.


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If this is all we want to print out, press Menu - File- and choose among the various print options.

Print Diagram: As the name implies, this will only print thediagram(s)Print Program and Events: Will print all the program steps andevents like opening and closing the lid, pressing manual seeding,

stop, etc.Print both of the above: Again, as the name already implies, bothof the above, diagram(s), program steps and events will bedisplayed on screen and eventually be printed if you desire so.

If you choose Print both of the above, the result might look something likethe display below.


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List of Available Tools

The size of the graphicaldisplay can now bemodified using the three

buttons shown.

The arrow buttons work like buttons on a tape recorder. The two middlebuttons move through the various pages, page by page, whereas the two

outer buttons move the display either to the first page or the last page. Someof you might have only one page to display. In this case, the arrow buttonsare not used.

The printer sign on the left is used for the printer - setup. The diagrams can beprinted out locally or be sent to a remote (network) printer.

A message (description) appears when the cursor is positioned over an icon.

Closereverts back to the main screen.

Show description, Show program, Display Total ViewIn the sub-menu Main Menu - File - Open historical data - Menu, we have the possibilityto look into the Freezing Prozess Information (Show description), look at the originalprogram curve (Show program) and display the complete program curve and processfile (Display Total View). Naturally, a process file must be opened first, using File -Prozess directory (choose your process record) or Open last run in order to display thelast freezing process data.We have now briefly discussed the Edit description and Open historical data menus.

The next menu Freezing programwill allow us to write a newor editan existing program.


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Create / Edit freezing program

As the name already implies, here we can create a new program or edit an existing program. Actually, weare getting again in the program edit screen as seen below.

When Create new is selected, a new program with onlytwo steps (0 and 1) will initially be displayed. Step #0represents the start temperature. It is the temperature, atwhich the sample should be loaded into the freezingcompartment. In many applications, this will be either 20Cfor ambient temperature or about +4C for refrigerator conditions. Thedefault starting temperature value is +20C.

Also, as a default, the slope in step #1 (temperature gradient) will initially be set at -1.00C/min.In order to edit an existing program, we would choose File / Edit existing and select the program wewould like to edit.

More on this, further down in this manual.

Set data directory

The process file of a finished run has to be saved somewhere. Here,we have the possibility to choose an appropriate location. For clarity

and ease of locating your data later on, we suggest putting the datasub directory in the same location as the IceCube freezing program.Assuming that you installed the IceCube program in a directory calledFreezer, a data directory called Process Recordswill be created.In this Process Record directory, all future process records (data) willbe saved.Note: In order to prevent data from being lost, do not forget to backupyour process records periodically.


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Any other directory can be used. Also a new directory can be created.

Note: Some expressions appear in German language. This is due the German WindowsOperating System installed. These expressions are replaced by similar words depending onthe Windows language version used.

Set program directory

As already mentioned above in Set data directory, the same applies here. Yousimply can choose the location where to store your freezing programs. Again,we suggest storing the freezing programs in a sub directory of your IceCubeprogram directory.But again, any other directory can be used. Also a new directory canbe created.

Note:To avoid loss of data and programs, it is advisable to makeoccasional back-ups.


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Exit

The last file menu entry is Exit. As in most other Windows basedprograms, Exit will close all processes within the program and revertsback to the desktop area. All running processes must be stopped or

finished otherwise, you will not be allowed to Exitthe IceCube program.

Note: To avoid loss of data and programs, it is advisable to make occasional back-ups.


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Main Menu - Display

In this menu, we can set the way the program curve will be presented onthe screen during a freezing run. Three sub-menus are available, TotalView, Tracking Viewand Settings.

Total View

Total Viewwill give us an overall view of the program temperature. This iscurrently between + 60C to -200C maximum. However, if the last step inthe freezing program ends at e.g. -120C, Total View will show a screenwith the lower temperature limit set at -120C.

Tracking View

Tracking View will follow the temperature curve as the program run progresses. This will give us anoptimal presentation of data on the display.

Settings

The remaining Menu entry is Settings.

Settingallows us to define how the screen behaves during a freezing run. A freezing run may need a longtime, thus requiring drawing curves beyond just one display screen. So we have totell the program at what stage to update the screen contents for both temperatureand time axis.In addition, we can choose if we want to get the elapsed or remaining timedisplayed in the instrument panel.Also, we can choose to get the temperature displayed on one of the temperaturecurves.

Time axis

On the left side of the screen, the time axiscan be set. In our example,during a program run, a time window of 18 minutes will be shown. Whenthe temperature curve approaches the right edge of the display area, just0.5 minutes before, the screen will shift to the right by 1 minute.


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Date post:	08-Apr-2018
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14S Operator Manual

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