Date post: | 11-Feb-2018 |
Category: |
Documents |
Upload: | manea-mihaela |
View: | 218 times |
Download: | 0 times |
of 27
7/23/2019 2 Members of the Family Enterobacteriaceae
1/27
MEMBERS OF THE FAMILY
ENTEROBACTERIACEAE
7/23/2019 2 Members of the Family Enterobacteriaceae
2/27
Citrobacter
Resembles Salmonella but are ONPG (+)& LDC (-)
Citrobacter speciesfreundii(!"# pneumonia# intraabdominal
abscess)
di$ersus(neonatal menin%itis)
amalonaticus(etraintestinal infections)
C' freundiiS' di$ersusS+-
"ndole-+
*CN+-
7/23/2019 2 Members of the Family Enterobacteriaceae
3/27
Klebsiella
Klebsiella -Enterobacter-Serratia-HafniaK. pneumoniae
,riedlanders bacilli (encapsulated andmucoid)
(+) Strin% test
Klebsiella aerogenes is associated with hospitalacquired infections of woundsand of t.e urinarytract
K. rhinoschleromatis/ r.inoscleromatis(%ranulomatous disease of t.e nose and p.ar0n
1it. intense s1ellin% and malformation of t.e entireface and nec2)
7/23/2019 2 Members of the Family Enterobacteriaceae
4/27
The patient'sK. pneumoniaeisolate
was string test positive (>5 mm
string length).
7/23/2019 2 Members of the Family Enterobacteriaceae
5/27
LABORAOR! "#A$%OSES
Specimens: Depending on the site of infection, specimens
include urine# pus# sputum and infected tissue'
&orpholo'y *lebsiellae are Gram ne%ati$e# non-motile# usuall0
capsulated rods'
(ulture
*lebsiellae are aerobes and facultati$e anaerobes'
Blood agar: Klebsiellae produce large grey white usuall0 mucoidcolonies'
MacConkey agar and CLED medium: Most 2lebsiellae are lactose-
fermentin%# producin% mucoid pin2 colonies on 3acCon2e0 a%ar and
0ello1 mucoid colonies on CL4D medium
K. rhinoscleromatis is non-lactose fermentin%' Biochemical tests
*lebsiellae are indole ne%ati$e# ornit.ine decarbo0lase ne%ati$e and do
not produce S'
7/23/2019 2 Members of the Family Enterobacteriaceae
6/27
Klebsiella on Macconkeymedium show largemucoid pink lactosefermenting colony
Klebsiella on CLED medium show
ellow la!tose "ermenting !olon
7/23/2019 2 Members of the Family Enterobacteriaceae
7/27
Enterobacter
LDC5DODCrease6ello1pi%ment
4' cloacae-++ 7-
4' aero%enes+-+ --4' %er%o$iae+-+ +-4' sa2a8a2ii-++ -+
4' ta0lorae-++ --
#spe!ies$ habitat$ soil% water% dair produ!ts
#normal "lora o" the gut o" animals &
humans
#motile% DC & * (+)
7/23/2019 2 Members of the Family Enterobacteriaceae
8/27
Enterobacter
7/23/2019 2 Members of the Family Enterobacteriaceae
9/27
Edwardsiella
4d1ardsiellatarda (.uman pat.o%en /
bacteremia and 1ound infection).os.inae (sna2es# birds and
1ater)
ictaluri (enteric septicemia infis.)
7/23/2019 2 Members of the Family Enterobacteriaceae
10/27
Serratia
opportunistic pat.o%ens
DN5se# lipase# %elatinase
9S' marcescens9S' li:uefaciens
9S' rubidaea
9S' oderifera
7/23/2019 2 Members of the Family Enterobacteriaceae
11/27
Serratia
7/23/2019 2 Members of the Family Enterobacteriaceae
12/27
Proteus
Tow important species , P. mirabilis and P.
vulgaris
&orpholo'y Proteus is actively motile with peritricous
flagella , non capsulate, Gram ne%ati$e
pleomorp.ic rods'
Rapid urease producers S1armin%# burned c.ocolate odor
7/23/2019 2 Members of the Family Enterobacteriaceae
13/27
Proteus with peritricousfagella
7/23/2019 2 Members of the Family Enterobacteriaceae
14/27
)atho'enicity
P. mirabilis causes:;- *rinary infections' in the elderl0 and
0oun% males and often follo1in%
cat.eteri8ation or c0stoscop0''
- Abdominal and wound infections
7/23/2019 2 Members of the Family Enterobacteriaceae
15/27
(ultureaerobically most Proteus cultures have a characteristic fishy Odour
and S1armin% on t.e medium
MacConkey CLED !LD media: non-lactose fermentin%colonies after o$erni%.t incubation at /
7/23/2019 2 Members of the Family Enterobacteriaceae
16/27
Proteus
P' mirabilis P' $ul%aris
"ndole-+
ODC+-
,ermentation 3altose - +
A0lose + + Salicin - +
C.loramp.enicolSR
7/23/2019 2 Members of the Family Enterobacteriaceae
17/27
Proteus
P' mirabilis P' $ul%aris
"ndoleBBB BB'
3altoseBBBBB'
A0loseBB''BB'
SalicinBB''BB'
C.loramp.enicolBB''BB
7/23/2019 2 Members of the Family Enterobacteriaceae
18/27
Proteus
)roteus3cCon2e0 rease test
7/23/2019 2 Members of the Family Enterobacteriaceae
19/27
Swarming on Blood
agar,warming pehomenon
7/23/2019 2 Members of the Family Enterobacteriaceae
20/27
Morganella
,ormerl 2no1n as Proteus
3' mor%anii / onl0 specie
!"s and 1ound infections
7/23/2019 2 Members of the Family Enterobacteriaceae
21/27
Providencia
Pro$idencia ( = species / P' alcalifaciens#
P' Rusti%ianii# P' rett%eri# P' stuartii)
P' rett%eri P' stuartii rease + -
Citrate + +
7/23/2019 2 Members of the Family Enterobacteriaceae
22/27
Yersinia
;; species6' pestis (pla%ue) / bipolar stainin% (1a0son)5%ent of bubonic pla%ue# pneumonic and septicemic pla%ues
9 ubonic / bite of infected flea9 Pneumonic
9 Prefered %ro1t. at > de% C6' enterocolitica (acute enteritis / appendictis li2e)
9 Pi%# cats and do%s9 Cold enric.ment9 3otile at R!
6' pseudotuberculosis
9 Pat.o%en in animals (tur2e0# %eese# do$es# farm anddomestic animals)
7/23/2019 2 Members of the Family Enterobacteriaceae
23/27
YERSINIA
et0 pin appearance
Yersinia pestis
7/23/2019 2 Members of the Family Enterobacteriaceae
24/27
evision !uestion ,- (haracteristic of enterobacterceae all of the
followin' ecept a- $ram ne'ati+e rod
b- /acultati+e anaerobic
c- /erment 'lucose
d- Oidase positi+e
0-Swormin' is special characteristic of
a. )seudomonades
b- )roteus
c- E. coli
d- Klebsiella 1- &ucoid colony is special characteristic of
a. )seudomonades
b- )roteus
c- E. coli
d- Klebsiella
7/23/2019 2 Members of the Family Enterobacteriaceae
25/27
2- Bacteria which 'i+e 'reen metallic sheen on E&B medium
is
a. )seudomonades
b- )roteus
c- E. coli d- Klebsiella
3- )olysaccharide capsule present on which of the followin'
bacteria
a. )seudomonades
b- )roteus c- Shi'ella
d- Klebsiella
4- 5hich of the followin' bacteria is urease positi+e
a. )seudomonades
b- )roteus c- E. coli
d- Klebsiella
6- 5hich of the followin' bacteria is lactose fermenter
a. )seudomonades
b- )roteus c- E. coli
7/23/2019 2 Members of the Family Enterobacteriaceae
26/27
5hen we inoculate members of
enterobacterceae on &ac(on7ey a'ar8
mention which or'anism 'row producin'
lactose fermentor colony and which 'row
producin' lactose fermentor colony
7/23/2019 2 Members of the Family Enterobacteriaceae
27/27
klebsiellaand e.!oli