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presenter : Guan Yu Chen

Date : 2011 / 12/29

Optimization of phycocyanin extraction from Spirulina platensis using factorial design S.T . Silveira et al ./ Bioresource Technology(2007)

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OUTLINEIntroductionMaterial and Method Result and discussionConclusionFuture work

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INTRODUCTION The Spirulina platensis is an excellent source of

phycocyanin , The protein fraction may contain up to 20% of phycocyanin

phycobiliproteins

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Phycocyanin has been used mainly as a food pigment and cosmetic, recently , it’s also has shown the anti-inflammatory and anti-cancer properties

This paper show the effects of temperature and biomass-solvent ratio on phycocyanin concentration and extract purity to determine the optimum conditions for phycocyanin extraction

INTRODUCTION

MATERIAL AND METHOD Spirulina platensis LEB 52 different solvents for Phycocyanin extraction : distilled water

10 mM sodium phosphate bu er(pH 7.0)ff

10 mM sodium acetate bu er (pH 5.0)ff

0.15 M NaCl

CaCl2 10 g /L

Extraction procedure

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mixing

dry biomass with solvent in a rotary

shaker at 30 °C

After centrifugation,

take the supernatant

Optical Density

was measured at 615 and 652 nm

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Formula

where PC is the phycocyanin concentration (mg/ ml) OD615 is the optical density at 615 nm OD652 is the optical density at 652 nm

where EP is the extract purity

MATERIAL AND METHOD

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RESULT OF EXTRACTION WITH DIFFERENT SOLVENTS

pH and ionic strength lead to affect phycocyanin extraction

near the isoelectric point ( pH5.11)

protein interaction

extraction amount

It was observed that extraction with water can be a good choice

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RESULT OF VARIABLE EFFECT ON PC & EP

temperaturebiomass / solvent

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RESULT OF VARIABLE ANALYSIS

The variable biomass-solvent ratio strongly influenced the phycocyanin concentration

As ratio phycocyanin concentration

it makes the volume of extractant

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RESULT OF VARIABLE ANALYSIS

PC model

The analysis of variance (ANOVA) was used to evaluate the adequacy of the fitThe F-value indicate the precision for the PC modle

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temperature purity because high temperature facilitated the extraction of other proteins

RESULT OF VARIABLE ANALYSIS

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The F-value indicate the precision for the EP modle

RESULT OF VARIABLE ANALYSIS

EP model

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CONCLUSION Water was chosen as the extractant, because it produced

high phycocyanin concentration and its low cost

The condition established for phycocyanin extraction was a biomass-solvent ratio of 0.08 g/ml, for 4 hrs, 25 °C

Under these conditions it’s possible to reach a phycocyanin concentration of 3.68 mg/ml and purity ratio (A615/A280) of 0.46

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I can follow the extraction procedure and adjust the variables to see what is the result of phycocyanin concentration

FUTURE WORK

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THANKS FOR YOUR ATTENTION