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3b- Microtools: Culturing

Basic methods for studying microbes

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Inoculating needle & loop

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Streak plate

Pour plate With loop dilution

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pipet & pipetters

Pour plate with measured dilution

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tubes

Petri plate

flask

Containers

For beer

Bioreactors/Fermentors

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agar broth agar

slant deep

agar

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(for “solid”: just add agar to 1-5%)

General medium eg: nutrient agar

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Enriched medium (for fastidious microbes)

eg: Blood agar Chocolate agar

Selective medium

Differential medium

For some species this one may be selective, for others it may be differential

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Differential media (newer)

Reducing medium eg: Fluid thioglycollate medium (FTM)

Aerobe anaerobe

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Media which are both Selective and Differential

MacConkey agar

Mannitol Salt agar

Basic methods for studying microbes