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3b- Microtools: Culturing
Basic methods for studying microbes
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Inoculating needle & loop
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Streak plate
Pour plate With loop dilution
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pipet & pipetters
Pour plate with measured dilution
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tubes
Petri plate
flask
Containers
For beer
Bioreactors/Fermentors
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agar broth agar
slant deep
agar
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(for “solid”: just add agar to 1-5%)
General medium eg: nutrient agar
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Enriched medium (for fastidious microbes)
eg: Blood agar Chocolate agar
Selective medium
Differential medium
For some species this one may be selective, for others it may be differential
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Differential media (newer)
Reducing medium eg: Fluid thioglycollate medium (FTM)
Aerobe anaerobe
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Media which are both Selective and Differential
MacConkey agar
Mannitol Salt agar
Basic methods for studying microbes