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3M products for Food safety solution © 3M 2009. All Rights Reserved. Le Thi Cam Tu – Technical Service Support 15 – 16 Aug 2012
3M products for Food safety solution
© 3M 2009. All Rights Reserved.
Le Thi Cam Tu – Technical Service Support
15 – 16 Aug 2012
1) Indicator Organism Testing => Petrifilm
2) Pathogen & Toxin Testing => Tecra kit (Eli sa), MDS (DNA )
3) Rapid Hygiene Monitoring => ATP NG Machine, Pro-tec
PRODUCT CATALOGUE
Group Typical products
4) End product screening => MLS II Machin e
5) Sample Handling & Media => Broth, Sponge
PETRIFILM TECRA - ELISA MDS - DNA Detection
PRODUCT CATALOGUEPlease visit our website :http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety
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3M3MTMTM PetrifilmPetrifilmTMTM PlatesPlates
Indicator Organism Testing
Only 3 steps :
Incubate CountInoculate
Indicator Organism Testing
Direct Plating 1ml :
Water testing
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Plating with Filter :
1 hr
Indicator Organism Testing
EnterobacteriaceaeYeast & Mould Rapid Coliform
ColiformsAerobic
Indicator Organism Testing
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E. coli & coliformsSelect E. coliStaph Express Evironment Listeria
High sensitive Coliforms
Indicator Organism Testing
http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-information/regulatory/
Regulatory information
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Indicator Organism Testing
http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-information/regulatory/
Regulatory information
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Indicator Organism Testing
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1) AC (Aerobic count)
� All bacteria growth with presence of
Oxygen.
� 48h
� Count red colonies.
� AOAC 986.33; 989.10; 990.12
Indicator Organism Testing
� AOAC 986.33; 989.10; 990.12
2) CC (Coliforms count)
� VRB (Violet Red Bile agar)
� 24h
� Count red colonies with gaz
� AOAC 986.33; 989.10; 2000.15
3) EC/CC (E.coli / Coliforms count)
� 24h – 48h
� Red colonies + gas : coliforms
� Red-blue colonies + gas : E.coli
� AOAC 991.14 ; 998.08
Indicator Organism Testing
4) SEC (Selective E.coli)
� 24h
� Blue colonies
Indicator Organism Testing
5) EL (Environment Listeria)� 31h
� Red - violet colonies
� AOAC PTM
6) STX (Staphylococcus express)
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6) STX (Staphylococcus express)
� 24h
� Purple colonies
� AOAC 2003.07; 2003.08; 2003.11*** In case there are green/black colonies, insert
additional disk and incubate 3h more and read colonies
with pink zone as positive colonies.
Indicator Organism Testing
7) RCC (Rapid Coliforms count)� 6h – 14h
� Red colonies with gas
� AOAC 2000.15*** Early appear yellow acid zone at 6h
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8) High sensitive Coliforms
� 5ml sample => sensitivity 2 cfu /g
� 24h
� Red colonies with gas
� AOAC 996.02
Indicator Organism Testing
9) EB (Enterobacteriaceae) � 24h
� Red colonies with gas or with yellow
acid zone or with both.
� AOAC 2003.01
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10) YM (Yeast and Mold)
� 3 – 5 days
� Yeast : small colonies with defined
edge, uniform color (blue/green).
� Mold : large colonies with diffused
edge, dark center, varied in color.
� AOAC 997.02
Indicator Organism Testing
3M™ Petrifilm™ Plate
Applied for Environmental Monitoring
1) Air Sampling Method
Hydrate plate with 1ml sterile diluent,
let gel solidified
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2) Direct contact Method
3) Swab Method
Incubate
Indicator Organism Testing
Storage condition :
• Before opened : < / = 8oC until 18 months after manufacturing date.
• After opened : seal pouch with tape, store at room temperature and use within 1 month.
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use within 1 month.
Indicator Organism Testing
Summary testing procedure by Petrifilm vs tradition al agar:
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Indicator Organism Testing
Summary FAB (Features, Advantages, Benefits) of Pet rifilm :
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Indicator Organism Testing
Source for documents of Petrifilm :http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-applications/one/
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For more information, please kindly contact :
Msc. Le Thi Cam Tu – Technical support3M Food Safety Division
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3M Food Safety Division3M Vietnam Ltd,77 Hoang Van Thai – Tan Phu ward – Dist 7 – HCM City
Email : [email protected]
Tel : 09 33 64 01 18
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