Date post: | 13-Dec-2014 |
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Technology |
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Massimo Pindo, PhD Sequencing & Genotyping Platform [email protected] Edmund Mach Foundation
Sequencing andGenotyping Platform
ApplicationsFacilities & Services
Edmund Mach Foundation (since 2007)Inheritance of Ist. Agrario San Michele a/A since
1874
A non for profit organization with private legal status and over 750 staff operating in several sites in the Trentino province
It provides Research, Education,Technology Transfer and Advisory Service in the Agri-Food and Environmental sectors.
FEM Research and Innovation Centre
Mission: The Sequencing and Genotyping Platform takes part in the research and development activity of the CRI by offering support at the researchers activity and with the development of new methods of experimental investigation. The first purpose of the Platform is the development of new investigative methods, with the acquisition of new knowledge in the green biotechnology field and the continuous improvement of present and future instruments.
Platform Services Platform facilities Platform Equipment
Germplasm platform
Apple:- 13.4 ha for apple breeding + germplasm collection- 1,200 accessions (wild and cultivated spp.)- 54,000 genotypes under evaluation /several mapping population (quality and pyramidation of resistances)- 8,000 genotypes in greenhouse
Grape: - 4.5 ha for grapevine breeding + germplasm collection
- 1,900 accessions (wild, hybrids and cultivated)- 12,000 new crosses (crosses, selected individuals or large mapping populations) resistances with high quality standards
Softfruits: - 2.5 ha for raspberry & blueberry breeding + germplasm - 104 strawbeery accessions - 140 blueberry accessions (20,000 seedlings/48 crosses) - 240 raspberry accessions (14,300 seedlings/110 crosses)
Genomics and Biology of Fruit Crops
CROSS SELECTED FOR: GENE
12.1 disesase resistance Vf Pl1
12.3 texture Md-PG1
12.4 texture and ethylene Md-PG1 Md-ACO1
12.5 disesase resistance Vf
12.6 disesase resistance Vf
12.8 disesase resistance Vf
12.9 ethylene Md-ACO1
12.10 disesase resistance Vf
12.11 texture Md-PG1
12.12 ethylene Md-ACO1
12.14 disesase resistance Vh4
12.15 disesase resistance Vh2
12.16 disesase resistance and texture Vf Md-PG1
12.17 disesase resistance Vf Vr2
12.18 texture Md-PG1
12.19 disesase resistance Vf 12.20 disesase resistance Vf Pl1
12.21disesase resistance and
ethyleneVf Md-ACO1
12.24 disesase resistance Vf Vr2
12.27 ethylene Md-ACO1
12.28 disesase resistance and texture Vf Md-PG1
12.29 disesase resistance Vf
12.30 ethylene Md-ACS1 12.31 ethylene Md-ACO1
MAS 2013 AT FEM24 APPLE CROSSES
6.471 PLANTS - 8.068 GENOTYPING ASSAY
Plants with desiderable traits
SAMPLES TESTED
69,51%
MASTER TRAITS(ie: Resistance, Texture,
Storage)
Marker Assisted Breeding at FEM
- MAB consultant (management system)
- gold standard markers - well optimazed flowthrough
STEP 1Seedling layout
STEP 2Sample collection
STEP 3Run Analysis
Marker Assisted Breeding at FEM
Next Gen. Sequencing
Whole Genome Seq.Target Reseq.MetagenomicsTranscriptome Seq.
Next Gen. Sequencing
Whole Genome Seq.Target Reseq.MetagenomicsTranscriptome Seq.
Genotyping &Robotic Platform
DNA & RNA extractionCapillary SequencingMid- to High-plex Genotyping
Genotyping &Robotic Platform
DNA & RNA extractionCapillary SequencingMid- to High-plex Genotyping
Next Gen. Sequencing and Protocol Setting
Shot gun & PE gDNA libraryAmplicon LibraryNext Gen. Sequencing Application
Next Gen. Sequencing and Protocol Setting
Shot gun & PE gDNA libraryAmplicon LibraryNext Gen. Sequencing Application
Massimo Pindo
Sequencing andGenotyping
Platform
Erika Stefani Daniela Nicolini
Simone Larger
People:
Elisa Banchi Lara Poles