A Contract Research and Services Organization

Ideas to Life!

A Contract Research and Services Organization

Xcelris is a Specialty Contract Research and Services Organization

Delivering the best end to end solutions to global Genomics, Pharma and Bio-Pharma Community

Built by team of Scientists and Management Professionals

Outstanding infrastructure facilities to support Cutting Edge Research

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Our Vision

“To  lead the way to a healthier world by partnering with Pharma & Biotech Community across the globe and delivering high quality value added healthcare solutions.”

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Mission

“Xcelris endeavors to be a world class Lifesciences based innovative service organization built towards achieving excellence in;

Research & Technology

Service Delivery

Management Capability”

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Values

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Infrastructure

cGLP and cGMP compliant Laboratory designed in accordance with global standards

Spread across 50,000 square ft. area

Fully equipped Labs conforming to global norms

State–of–the–art equipments

Dedicated LIMS

Dedicated lab for Genomics and Bioinformatics.

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People

Committed & enthusiastic high performing team of 50 members

More than 100 man-years of experience in Molecular Biology, Plant Molecular Biology, Genome Sequencing, Animal Genetics, Virology, Bioinformatics, Biotech and analytics

Alliance partner having experienced people from premier institutions like Sanger Labs, Baylor College which were part of HGP.

Xcelris Scientific Board with a strong experience in the field of Genomics & Biopharmaceuticals.

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Ideas to Life!

Whole Genome Sequencing

Next Generation Sequencing Technology Platforms using ABI & Roche

Whole Transcriptome Analysis and SNP Discovery

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Next Generation DNA Sequencing Technology

SOLiD technology: Two slide per run and up 16 samples /run. 2 Base encoding with higher fidelity. 50-75 base per run. 10-15 GB data per run.

Phase I for ABI SOLiD: Wet Lab

Preparation of high quality DNA.

Qualification of DNA using AGE/Picogreen.

DNA Shearing and End repair.

Fragment Library or Mate Paired Library.

Clonal Emulsion PCR and Bead Enhancement.

Bead Deposition and Ligation Sequencing.

Raw data Generation.

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Whole Transcriptome Analysis

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Xcelris Capability

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Xcelris Capability

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ABI SOLiD Technoloy

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ABI SOLiD Technoloy

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GS FLX technology

Phase I for GS FLX: Wet Lab

Sample Type: Genomic DNA, PCR product, BAC and cDNA.

Qualification of DNA using AGE/Picogreen.

DNA fragmentation 300-800 bp and End repair and adaptor A&B ligation.

Water-in-oil emulsification of beads and PCR reagents.

Clonal amplification in microrector.

DNA Bead Deposition to Picotitre plate and Pyrosequencing.

Raw data Generation.

One slide per run and up 16 samples /run. 300-400 bp per read. 1.2 million reads per run 0.8-1.0 GB data per run.

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Roche GS FLX (454) Technology

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Roche GS FLX (454) Technology

Sequencing Workflow : Loading of Pico Titer Plate Device

Well diameter: average of 44 µm> 400,000 reads obtained in parallel

A single clonally amplified sstDNA bead is deposited per well

Depositing DNA beads into the Pico Titer Plate device

Quality filtered bases

Amplified sstDNA library beads

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Roche GS FLX (454) Technology

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Roche GS FLX (454) Technology

A A T C G G C A T G C T A A A A G T C A

C TA

Repeated dNTP flow sequence:

GGTCAGTCAGTTTTCAG GAT CCCGATT

G CT A

Anneal Primer

Process continues until user-defined number of nucleotide flow cycles are completed.

Sequencing-by-synthesis

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Xcelris Capability

Phase IV: Bioinformatics

Assembly and Annotation.

Location of genes/promoters

Transcribed Regions

Function of Motifs/other DNA seq

Screening of data against bacteria/virus and vector data bases.

Detection of sequence variations

SNP/CNV/Dupli/In-Del

Phase II: Data processing

Genome Data processing,

Align & Create color space consensus

Convert color space consensus to base space for SOLiD.

Draft Assembly.

Final Assembly and Annotation.Removal/Identification of the gaps.

Phase III: Genome Finishing

Gap closure by PCR-assisted contig extension (PACE) methodology. Contig Gap filling and customized

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ABI SOLiD Technoloy

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Whole Transcriptome Data Analysis

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Deliverables

 Alignment Report -A summary of the mapping results, including score distribution.

  Filter report (Optional)    Score distribution plots   MAX file -A MAX file contains all the reads that map to genomic reference.

Does not include mapping to splice junctions.   General Feature Format (GFF) file -A GFF file containing all the reads that

were mapped uniquely, including those mapped to splice junctions.   Counts file-A file in GFF format, containing information regarding the

number of reads hitting the corresponding region (Figure 2).    Annotation wig file-Coverage file in wiggle format for regions contained in

the annotation file, one for each genomic region (chromosome) -- Predicted Transcribed Region (PTR) GFF files

-- Optimization plots-These plots are used in selecting the optimal parameters for detecting Novel Transcribed Regions (NTR).

-- Annotated Transcribed Regions (ATR) plots  

Thanks...