advanced technologies for Regenerative Medicine · FOR PRODUCT INFORMATION 508.893.8999...

advanced technologies for

RegenerativeMedicine

from Research to Clinical

Injection & Perfusion

Bioreactors

Biosensing

Total Solutions

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BehavioralResearch

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FOR PRODUCT INFORMATION 508.893.8999 [email protected] www.harvardapparatus.com

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INTRODUCTION

NEW Breakthrough ProductsRegenerative Medicine

InBreath 3D Hollow Organ Bioreactor, proven through human transplantation

Clinically proven and published bioreactor design used in successful first-in-humantransplantation of bronchus. Produced in conjunction with Politecnico di Milano and University of Barcelona, the InBreath bioreactor is ideally suited to regenerationof tubular hollow organs such as trachea/bronchus, blood vessels, esophagus and intestines.

See page 43.

Sterilizable pH, CO2, O2 and Glucose SensorsBlood gas and metabolite measurements are critical determinants of organ and tissue viability and performance. These sensors are available in a range of sizes andconfigurations as well as bioreactor-integrated forms for online monitoring. Sterilizableand made of completely inert material, these sensors are ideal for bioreactor use.

See page 81.

Maintain Stem Cell Viability & Functionality with Cooled Cell Injection Pump

Developed in the field, specifically for repeat injections of 1 µl to 100 µl of stem cells into live tissue. Easy-to-use, the NanoCool can be handheld or stereotaxic mounted for injections into live tissue while maintaining cell viability and functionality. TheNanoCool cools cells to 18°C which results in a substantial reduction in O2 consumptionas compared to 23°C.

See page 13.

Hand-Held Microscope up to 500X MagnificationSmall, light weight digital microscope for Organ and Tissue preparations, microsurgery, injection or cannulation. Remotely placed monitor (or PC Connection)enables simple mounting to bioreactor-based systems. Simple USB connnection. Aneconomical solution in a huge range of configurations of magnification, illuminationand functionality.

See page 86.

See actual experimental implementations of our products featured on Nova Science Now and a Reuters news report.

To view these video’s, use your smart phone to scan QR codes to the right or visit www.harvardapparatus.com.

Video Experimenton NOVA

Reuters News Report

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Note: Products in this catalog are for Research Use Only. Not for use on humans unless proper investigational device regulations have been followed.


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INTRODUCTION

NEW Breakthrough Products for Regenerative Medicine

InBreath 3D Hollow Organ Bioreactor, Proven Through Human Transplantation

Clinically proven and published bioreactor design used in successful, first-in-human,transplantation of bronchus. Produced in conjunction with Politecnico di Milano and University of Barcelona, the InBreath Bioreactor is ideally suited for the regeneration of tubular hollow organs such as trachea/bronchus, blood vessels,esophagus and intestines.

See page 37.

Sterilizable pH, CO2, O2 and Glucose SensorsBlood gas and metabolite measurements are critical determinants of organ and tissue viability and performance. These sensors are available in a range of sizes andconfigurations as well as bioreactor-integrated forms for online monitoring. Sterilizableand made of completely inert material, these sensors are ideal for bioreactor use.

See page 69.

Maintain Stem Cell Viability & Functionality with Cooled Cell Injection Pump

Developed in the field, specifically for repeat injections of 1 µl to 100 µl of stem cells into live tissue. Easy-to-use, the NanoCool can be handheld or stereotaxic mounted for injections into live tissue while maintaining cell viability and functionality. TheNanoCool cools cells to 18°C which results in a substantial reduction in O2 consumptionas compared to 23°C.

See page 9.

Hand-Held Microscope up to 500X MagnificationSmall, light weight, digital microscope for organ and tissue preparations, microsurgery, injection or cannulation. Remotely placed monitor (or PC Connection)enables simple mounting to bioreactor-based systems. Simple USB connnection. Aneconomical solution for a huge range of magnification configurations, illumination andfunctionality.

See page 65.

See actual experimental implementations of our products featured on Nova Science Now and a Reuters news report.

To view these video’s, use your smart phone to scan QR codes to the right or visit www.harvardapparatus.com.

Video Experimenton NOVA

Reuters News Report

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Pressure Controlled Infusion PumpNow a system to deliver pressure-controlled fluid injection to intact organs andtissues. The system delivers rapid injections while simultaneously monitoringpressure at the injection site. Measured pressure modulates pump speed for tightcontrol of delivered injection pulse. Ideal for delivering a pressure controlledinjection.

See page 12.

Dedicated Lung Bioreactor with Online PulmonaryMonitoring used in Successful Lung Regenerationand Transplant

Proven and published whole organ bioreactor specifically designed in conjunctionwith Massachusetts General Hospital for generation of rodent lungs. Activeventilation and real-time monitoring of respiratory mechanics for organ performancevalidation and successful transplatation.

See page 39.

Imaging Multi-well Bioreactor/IncubatorNow accelerate your tissue, cell and organ research. 4, 8, 16, 24, 48, 96 well imagingplates on the microscope. 4 wells with active independent perfusion. Now performsimultaneous experiments in multi-well format for high throughput screening ofdecellularization and recellularization conditions.

See page 44.

Imaging Technique, IRCI (Infrared Contrast Imaging)Visualize tissue and organ metabolic activity, vascularization of tissue and diseaseconditions in a bioreactor or during cell therapy. The new IRCI technology allows youto visualize events and evaluate treatment site conditions providing a new andpowerful tool for regenerative medicine.

See page 76.

Developed with LeadingScientists and Surgeons!

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1FOR PRODUCT INFORMATION 508.893.8999 [email protected] www.harvardapparatus.com

In 2011, Harvard Apparatus was chosen as the #1 most Innovative Biotechnology Company inMassachusetts by the Boston Globe. To view press release, use your smart phone to scan theQR code or visit www.harvardbioscience.com/releases.cfm.

INTRODUCTION

Woven Synthetic Scaffold MaterialAccelerate tissue and cell engineering protocols with ready-made and precisely-defined scaffold sheets and nibs. Biocompatible and cell friendly polymer materialwith special configurations available. Works with multi-well bioreactors.

See page 60.

Ultra Microliter Injection SystemsThese zero dead volume, 40, 60, 80 micron needles delliver precision trace chemicalswith minimium tissue damage. Ideal for small volume injection utilizing the NanoCoolinjector either hand held or in a stereotaxic frame.

See page 11.

Do It Yourself Rugged Heparin Coating KitNow in your lab you can coat glass, plastic and metal with a rugged heparin coatingto reduce clot formation in bioreators, culture dishes, biomaterial and fluids. Lab SiteHeparin Coating is simple to apply while the selective removal process allows easyreversability.

See page 62.

A Family of Cell Friendly PumpsWhen pumping cell suspensions, these gentle pumps produce reduced cell damagecaused by conventional roller pumps. These advanced pump designs are ideal for celland 3D organ bioreactors.

See page 23.

NEW Regenerative MedicineResearch Breakthroughs

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Note: Products in this catalog are for Research Use Only. Not for use on humansunless proper investigational device regulations have been followed.

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Table of Contents

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Table of Contents for Regenerative Medicine

Company Information 4-5 Digital Gas Mixer 51-53

Harvard Apparatus In the News 6-7 HPC-3, Hydrostatic Perfusion Chamber 54

Injection and Perfusion 8 C-Pace Cell Culture EP Stimulator 55-57

Cell Injection System Poster fold out Stimulator C 58

NanoCool Cell Delivery System 9-10 Stimulator CS for Isolated Cells 59

UI Ultra-Microliter Injection System 11 Synthetic Scaffold Material 60-61

Pressure Controlled Injection System 12 Lab Site Heparin Coating 62

PHD ULTRA™ Gradient System 13 Flow Through Device Accessories 63

PHD ULTRA™ Electrospinning Scaffolds 14 Biosensing 64

PHD ULTRA™ Continuous Flow Syringe Pump 15 Biosensing Introduction 65

Fast Bolus Injector for Cell Therapy 16 PLUGSYS Modular Measuring Control System 66

Advanced Features of the PHD ULTRA™ Family 17-20 PowerLab Data Acquisition System 67-68

Pulsatile Blood Pumps 21-22 Polestar Florescence-based Biosensors 69-72

Centrifugal Pump for Blood 23 Universal Perfusion Solution Monitor 73

REGLO Digital/Analog Programmable Peristaltic Pumps 24 Handheld Microscopes 74-75

MCP Pump Drive 25 Infrared Contrast Imaging (IRCI) 76

Pump Head 380AD for Biologicals, Gentle Peristaltic 26 Infrared Contrast Imaging (IRCI) Poster fold out

SB Pumo Head for Standard Applications 27 IRCI Cameras 77-79

Gentle Pumping Pump Heads for MCP & BUP Pump Drives 28 IRCI Camera Selection Guide 80-81

Perfusion Accessories 29 Infrared Contrast Imaging Guide 82-85

SWS-10, SWS-60 & SWS-140 Syringe Warmers 30 IRCI Imaging Kidney and Lung 86

SC-20 Dual In-Line Solution Heater/Cooler 30 Visualizing Vascularization Changes 87

Guide to Pump Capabilities 31 Visualization of Vessel and Vascular Leaks During Surgery 88

Regenerative Medicine Pumping Technologies 32-33 Visualizing Aerosols 89

Bioreactors 34 Visualization of Water Flow 90

3D and Multi-Well Bioreactors 36 Emissivity, Enhanced Visualization with IRCI 91-93InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels 37-38 Visualize with ICI Imaging 94-95

LB-2 Bioreactor for Mouse and Rat Lung Regeneration 39-40 Total Solutions and Support 96

3D Bioreactor Poster fold out Regenerative Medicine Phases 97-98

Regenerated Trachea Poster fold out Solutions for Regenerative Medicine Applications 99-102

Lung Bioreactor Poster fold out Selected References 103-104

Infrared Contrast Imaging Poster fold out Index 105-108

KLB-2 Kidney and Liver Bioreactor 41

Linear Stress and Relaxation Bioreactor 42

High Precision 3-Axis Imaging Platform 43

Harvard Apparatus Accelerator 1000 Multi-Well 44-45

3-Axis Imaging Platform Poster fold out

Imaging Bioreactor and Incubator 46

RC-37F and RC-367W 47

RC-37FC and RC-36WC 48

Electric CO2 Microscope Stage Incubator 49

Electric CO2 Microscope Stage Incubator Accessories 50

Contact us anytime tospeak with one of our

technical experts.Email:

[email protected]

T E C H N I C A LS U P P O R T

Advanced Solutions for Regenerative Medicine & Tissue Engineering & Cell Therapy

Phase 1 Phase 2 Phase 3 Phase 4 Phase 5PREPARATION &HARVESTING

ENGINEERING &MODIFICATIONS

CULTURING &GROWTH

CONDITION MONITORING WEBSITE

Harvard Apparatus

• Identification Products• Ventilators• Anesthesia• Surgical Tools• Infusion Pumps

• Fast Bolus Injections• Cell Delivery Systems• Iontophoresis• Liposome Prep• Drug Delivery Systems• Capillary Glass

• 3D Bioreactors• Toxin & Chemical Removal • Perfusion Systems

• Live Imaging• Blood Gas Measurements• Metabolic Monitoring• Data Aquisition & Analysis• Feedback & Control

Syringes

web:www.harvardapparatus.comph: +1 508-893-8999

Denville Scientific

• Spinner Flasks • Centrifuge• Pipetting Systems• Incubators • Shakers• Sterilizers

• Transfection Reagents• Ligation Kits • Standards• Visualization Kits• DNA Polymerase • PCR

• Spinner Flasks • Centrifuges• Pipetting Systems• Incubators • Shakers

• Protein Measurements• ssDNA • RNA• Phosphorus• Enzyme Kinetics

web:www.denvillescientific.comph: +1 908-757-7577

Hugo Sachs Elektronik

• Ventilators • Operating Tables• Vital Signal Monitors

• Isolated Organ Baths• Tissue Baths• 3D Organ Bioreactors

• Electrophysiology• Cardiopulmonary Mechanics• Data Aquisition & Analysis

web: www.hugo-sachs.de/ ph: +49 76 65 - 92 00-0

Biochrom

• Molecular Spectroscopy• Protein Analysis• Oligonucleotide Primers• DNA Melts• Molecular Analysis • Amino Acids Analysis

• Molecular Spectroscopy• Media Monitoring

• Molecular Spectroscopy• Amino Acid Analyzers

web: www.biochrom.co.uk/ph: +44 (0) 1223 423723

Warner Instruments • Cell Imaging

• pH Monitoring• Spill Sensors• Perfusion Chambers

• Electrodes • Amplifiers• Perfusion Chambers• Imaging Chambers• Ion Channel Monitoring• Ussing Chambers• Temperature Control

web: www.warneronline.comph: +1 203-776-0664

Hoefer

• Molecular Analysis• Cell Membranes• Cytoplasm Toxin Measure• Cell Signaling Chemicals• Blotting

• Molecular Sample Prep• Electrophoresis • Affinity• Ion Exchange • SEC/GPC• Filtration • Dialysis

web:www.hoeferinc.com ph: +1 508-893-8999

BTX• Electroporation• Electrofusion• In-vivo Transfection• Pneumatic Injection

web: www.btxonline.com ph: +1 508-893-8999

Panlab/Coulbourn

• Behavioral Apparatus:- Mazes - Rota Rods- Treadmills- Activity Monitors

web: www.panlab.com ph: 34 934 750 697 web: www.coulbourn.comph: +1 610-395-3771

CMA • Microdialysis web: www.microdialysis.seph: +46-8-470 10 00

Harvard Bioscience companies have partneredwith leading global scientists to providespecialized solutions for over 110 years…andwe’re doing it again for Regenerative Medicine!

Harvard Apparatusand the HarvardBioscience family ofcompanies areuniquely positioned to

develop advanced instrumentation to accelerate TissueEngineering and Cell Therapy treatments in RegenerativeMedicine. From our origins in 1901, Harvard BioscienceCompanies have worked closely with leading globalresearchers across many disciplines to produce clinicaland research equipment with

– the highest performance

– unmatched quality

– expert support

necessary to meet the demands of cutting-edge lifescience techniques. In keeping with this tradition, we look forward to workingwith you to develop and supply the next generation oftools to solve the new challenges of RegenerativeMedicine from the research lab bench to the patient.There are thousands of publications in regenerativemedicine and stem cell research utilizing HarvardApparatus products and we are now introducing a fullrange of newly developed products including 3D OrganBioreactors for organ generation and small volume celldelivery systems for cell therapy. These unique productswill serve the researcher and the physician and acceleratethe translation of novel therapies from research to clinical practice.

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INTRODUCTION

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FOR PRODUCT INFORMATION 508.893.8999 [email protected] www.harvardapparatus.com 5

Harvard Apparatus is uniquely positioned todevelop new tools for Regenerative Medicine

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110 YEARSDESIGNINGspecialty physiology &electrophysiology apparatus,for small animals

INTERNAL EXPERTS & EXPERTISEin organisms, organs, tissueand cell physiology

LARGEST GLOBALRESEARCH USER BASEin specialty physiologyproducts

RELATIONSHIPSwith global scientific leaders: technology,research and policy

• Physiological sensors and devices

• Microscopic imaging chambers

• Isolated ogran baths

• Physiological acquisition and control

• Macro and micro fluidic 2D reactors

• 3D organ bioreactors

• Infusion pumps

• Ventilation systems

• Low Volume Fluidics

• Low Volume Sensors

• Respiratory Mechanics

• Isolated Organ Baths

• Cell Perfusion and Imaging

• Environmental Control: pH, Temperature, Nutrition

• Amplifiers, Filters and DataAcquistion

• Imaging Chambers

• Spectroscopy and Cell Injection, 3D Organ Bioreactors

• Hundreds of Isolated Organ andPerfusion systems in labsworldwide

• >100,000 physiological fludicdevices in use

• Familiar with global regulatorycompliance and manufacturingregulations

• GMP manufacturing in UK and US

• Global manufacturing centers inGermany, Spain, Sweden, UnitedKingdom and United States

• WEE and RoHS compliant, FDA QSRand ISO13485 Certified

• Largest catalog and globaldistribution in physiologicalspecialty research apparatus

• Sponsored Research Programs

• Exclusive Licences

• Research and Development

• Industrial Partnerships

• GMP Manufacturing

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Harvard Apparatus

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When Harvard Apparatus develops new products for anew market, we team up with leading scientists aroundthe world. We use their input to develop new toolsspecifcally designed to help solve real research and clinical problems.We are proud and grateful to be part of the successfulimplementation of these new research devices. Somenew tools have even been successfully used in humanmedical proceedures under approval of local governmentand Institutional Review Boards.Harvard Apparatus would like to thank our users forallowing us to be part of this exciting new field ofregenerative medicine and look forward to morecollaborations and breakthoughs in the future.

Harvard Bioscience’s Bioreactor Used to Produce a Synthetic Tissue-Engineered Airway Used in World’s First Successful Human Transplantation

Lab-Made Trachea Saves ManTumor-Blocked Windpipe Replaced UsingSynthetic Materials, Patient’s Own Cellls

Surgeons Carry Out FirstSynthetic Windpipe Transplant

Patient Gets World’s FirstArtificial Trachea

Lab-Made Organ Implanted forFirst Time

World’s First Laboratory-Grown Windpipe is

Transplanted in Patient

Stem Cells Grow NewWindpipe

INTRODUCTION

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First use of 3D organbioreactor to producesuccessful regenerative

medicine transplant

First in Man transplant ofregenerated human bronchi

with decellurized organ


in the News

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Harvard Bioscience 2010 Top BiotechnologyCompany in Massachusetts

Can We Live Forever?Replacing Body Parts.

How to Build a Beating Heart.

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INJECTION & PERFUSION

Zero Dead VolumeInjection Needle,see page 11

Cooled Injection System toMaintain Cell Viabilitysee pages 9-10

Automatic GradientComposition System,

see page 13

PHD ULTRA™ Push-PullProgrammable Syringe Pump

see page 15

solutions for regenerative medicine:

injection & perfusionTable of Contents

NanoCool™ Poster fold out between 8-9

NanoCool™ Cell Delivery System 9-10

UI Ultra-Microliter Injection System 11

Pressure Controlled Injection System 12

PHD ULTRA™ Gradient System 13

PHD ULTRA™ Electrospinning Scaffolds 14

PHD ULTRA™ Continous Flow Syringe Pump 15

Fast Bolus Injector for Cell Therapy 16

Advanced Features of the PHD ULTRA™ Family 17-20

Harvard Apparatus Pulsatile Blood Pumps 21-22

Centrifugal Pump for Blood 23

REGLO Digital/Analog Programmable Peristaltic Pumps 24

MCP Pump Drive 25

Pump Head 380AD 26

SB Pump Head for Standard Applications 27

Gentle Pumping Pump Heads for MCP Pump Drive 28Accessories

Micro Valves & Micro Connectors 29SWS-10, SWS-60 & SWS-140 Syringe Warmers 30SC-20 Dual In-line Solution Heater/Cooler 30

Guide to Pump Cababilities 31

Regenerative Medicine Pumping Technologies 32-33



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NEW NanoCool™ Cell Delivery SystemFixed 18˚C or Room Temperature for Optimal Cell Viability or Drug Delivery

When injecting stem cells,viability of cells can be amajor issue. The HarvardApparatus NanoCool™ is theonly cooled cell injectionsystem available. Celldelivery is totally integratedin its operation through theNanoCool’s microprocessorsystem. Power, temperatureinjection volumes and flowramping are all controlled bythe NanoCool’s program.Keeping the cells at 18°Cinstead of 37°C reduces O2consumption of cells by anaverage of 76%. The chartto the right (Figure 1) showsthe different organ utilizationcurves.

Temperature effect on O2 Consumption by Organ Cell Type

Organ Temperature OxygenComsuption

Change in O2Consumption % Change

Kidney 37 to 18 3.7 to 0.9 2.8 75.7Liver 37 to 18 3.0 to 0.6 2.4 80Heart 37 to 18 2.4 to 0.7 1.7 70.8Brain 37 to 18 1.9 to 0.4 1.5 78.9Muscle 37 to 18) 0.8 to 0.2 0.6 75Skin 37 to 18 0.4 to 0.1 0.3 75

NanoCool Hand-heldor Stereotaxic

Mounted Injector

NanoCool TouchScreen Controller

Foot-Pedal

Infrared Profile ofNanoCool InjectionSystem

KEY FEATURESSyringe contents maintained at 18°C or room

temperature for accurate spatial injections

Flow ramping to reduce injection site blowback

Programmed methods for automatic recall of entire cell

delivery protocol. Up to 100 methods stored for recall

2 year warranty. Certified with CE, UL, CB Scheme

High accuracy delivery between 1.3 pl/min to 68 ml/min

Preprogrammed bolus injection mode, just specify

injection size and time of dispense

18°C for optimal cell viability using a range of syringes

20% Increase in Survivability, 76% Lower O2

Consumption Rate at 18°C vs. 37°C

High Accuracy delivery between 367.4 pl/min

to 1.906 ml/min

Footswitch activation keeps hands free

Figure 1: Temperature effects

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Figure 1: The line above, clearly shows the high accuracy and smooth flow of the Nanomiteinjection compared to two optimal hand injections.

Figure 2: Tissue creates backpressure but flow is gently ramped so tissue has a chance toexpand to accommodate liquid displacement. Program a volume and time to be delivered,Nanomite does the rest.

NEW NanoCool™ Cell Delivery System (continued)

For Cell or Drug Delivery: Hand Held or Stereotaxic Mounted

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NanoCool™ Cell Delivery System

RM1 70-3040 NanoCool™ Injector Infusion/Withdrawal Programmable Single Syringe Syringe Pump

NanoCool™ Cell Delivery System Specifications

# OF SYRINGES 1

ACCURACY ±0.5%

CABLE LENGTH 6 ft (1.8 m)

CONTROLLER DIMENSIONS 4-1/2 x 9 x 4-1/2 in (11.4 x 22.9 x 11.4 cm) (H x W x D)

FLOW RATE MAXIMUM 1900 µl/min

FLOW RATE MINIMUM 3.3 nl/hr

INJECTOR HEAD/ACTUATOR 7 x 1-3/8 x 2 in (17.8 x 3.5 x 5.1 x cm) (L x H x W)

Pump Function Infusion, withdrawal, volume dispense or continuous pumping modes

REPRODUCIBILITY ±0.05%

SYRINGE DIAMETER (MAX) 6.00 mm

SYRINGE SIZE:

MAXIMUM 1 ml

MINIMUM 0.5 µl

SYRINGE TYPES 1700 , 7000 , 700 and 1000 Hamilton syringes, or anyother syringe manufacturer with known ID in mm

Stereotaxic Mounted



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UI Ultra-Microliter Injection SystemZero Dead Volume, Small Gauge, Small Volume Injections with Minimal Injection Site Damage

UI Ultra-Inject System

The UI Ultra-Microliter Injection System is a unique tool for thecontrolled injection of small cells or drugs into either soft tissue orlarge cells. The UI micropipette has a specially designed glass tipthat easily attaches to any Hamilton syringe for microinjection ofexperimental fluids.

The UI micropipette features a beveled tip for minimal tissue injury,direct and simple setup, and no dead volume during application. TheUI is available in tip sizes of 40, 60, and 80 microns to facilitateinjection of a range of sample sizes.

Attachment to any Hamilton syringe having a 26 GA needle isstraightforward: Insert the syringe needle into the pipette until itstops and you’re ready for secure, leak-free delivery.

Optionally, delivery volume can be well controlled using HarvardApparatus’ Pump 11 Elite Nanomite programmable syringe pump.Specifically, the pump head can be mounted to a micromanipulatorstereotaxic, or handheld for precision injection.

Ultra Inject mounted to 26 gauge needle

KEY FEATURESStem cell injection

Drug delivery

Nerve injury and regeneration

Muscle fibers

Large cell microinjection

Easy to use

Order # Product

UI Ultra-Microliter Injection System

RM1 64-1929 UI-40, Ultra Inject for 26 gauge needle, 40 micron tip



RM1 70-3601 Nanomite, Infuse/Withdraw Syringe Pump

UI Ultra-Microliter Injection System Specifications

TIP DIAMETER 40-80 microns beveled

MATERIAL Borosilicate glass

O.D. 1.5 mm

LENGTH 43 mm

SEAL SIZE Designed for use with 26 gauge Hamilton syringe needle

NON STERILE Recommended sterilization method is ethylene oxide gas


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Pressure Controlled Injection SystemFlow Delimited by Set Pressure

The system is specifically designed for repeatable, pressurecontrolled injections into intact organs. However a wide range ofsettings and accessories makes the system appropriate for virtualllyany semi-rigid substrate and certain hollow-body systems.

Figure 1: Pressure Delivery Injection System

KEY FEATURESDirectly measure pressure at site of injection

Simultaneously measure overall system pressure

Option for holding injection pressure constant

for defined time period

Order # Product

System Controller

RM1 77-0000 ML866 POWERLAB 4/304 Channel w/LABCHART Softwareto Measure System Pressure after Syringe

RM1 72-4496 Research Grade Blood Pressure Transducer, 115VAC/60Hz, with 6 ft cable

Pressure Measurement

RM1 72-9845 1F SGL Pres CTH 120 cm No Rep

RM1 70-3007 PHD ULTRA™ I/W Programmable

RM1 70-3033 PHD ULTRA™ Analog Central Imput

Constant Pressure Monitoring

RM1 73-1523 PLUGSYS Minicase Type 609

RM1 73-2806 SCP PLUGSYS Servo Control F/Perfusion with Interface Cablefor PHD ULTRA™ Control

RM1 73-0065 TAM-A HSE PLUGSYS Transducer Amplifier Module Type 705/1, Analog Display

RM1 72-9843 Pressure Catheter to Tam Cable

Accessories

RM1 72-2673 Y-Connector FLL/FLL/MLL (Rotating), Package of 25

RM1

72-14553-WAY

FLL-FLL-FLL "T" CONNECTOR, POLYPROPYLENE, PK/25

RM1 73-0500 Lab Stand with Heavy Triangular Base Plate 8 mm OD Rod, 30 cm Long. Also includes Acrylate. Block Clamp (73-0566)W/8 & 10 mm opening

RM1 72-9523 MLL to MLL Connector

RM1 77-0156 Manual Pressue Calibration

Figure 2: System components include a syringe pump, injection pathway, pressure measurementhardware, and data acquisition and feed back system. Injection parameters are programmedwhile pressure is continuously monitored. Real time pressure feedback modulates syringe pumpto keep pressure constant.


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PHD ULTRA™ Gradient SystemAutomatic % Composition Delivery of up to 3 Liquids

The new PHD ULTRA™ Gradient System is easily programmed for step and continuous concentration gradients from two or three solvents (Figure 1). Figure 2 shows the flow at 1000 and 500 µl/min. These serial dilutions or solution changes can bepreprogrammed or signaled and initiated by an I/O event. In thecase of injections you could automatically change the amount ofcells in every injection by programming an increase in theconcentration of cell solution in each injection or by simplyselecting a new pre-established method. In perfusions, a pH probecould signal for a different % composition of the perfusion streamor dose a perfusion stream automatically. This could provide morebuffering capacity or adjustment solution to the perfusion streamcompletely unattended.

Figure 2: 1000 nl/min & 500 nl/min with 250 µl syringeMeasured on a Sensirion Flow Sensor (PHD ULTRA™)

Figure 1: Stepped, Continuous, and Ramped gradient.

Figure 3: The following screen shows the set-up for a gradient.

KEY FEATURESEasily run binary or ternary gradients without a PC

Combine multiple flow streams into one common output

No stopping experiment to fill syringes with

different mixtures

APPLICATIONSSerial Dilutions of reaction dosing solutions

Serial Dilutions for drug infusion experiments

Serial Dilutions for nutritional infusion experiments

Serial Dilutions for mixing polymers in electrospinning

Chromatography

FIA systems

This new capability of Harvard Apparatus syringe pumps tocompletely deliver % composition changes automatically with highaccuracy and precision flow provides a new economical tool toadvance experiments and eliminate manual input for complexsolution changes.

- Enzyme studies

- Reaction dosing solutions

- Drug infusion experiments

- Nutritional infusion experiments

- Mixing polymer in electrospinning

- Chromatography & FIA systems

Order # Product

RM1 70-4101 PHD ULTRA™ Gradient System, 1 Master/1 Satellite with Stand

RM1 70-4102 PHD ULTRA™ Gradient System, 1 Master/2 Satellites with Stand

RM1 70-4106 PHD ULTRA™ Gradient System, 1 Master/1 Satellite without Stand

RM1 70-4107 PHD ULTRA™ Gradient System, 1 Master/1 Satellite without Stand


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PHD ULTRA™ Programmable Syringe PumpA Pump for Electrospinning a Novel Scaffold Fiber Materials

Electrospinning

Harvard Apparatus’ PHD Series of syringe pumps has the highestpublication rate out of any available syringe pump for electrospinning.In 1934, electrospinning was patented by Formhals, wherein anexperimental design was outlined for the publication of polymerfilaments using electrostatic force. Electrospinning is done by using ahigh voltage to create an electrically charged jet of polymer solution,or melt, which dries or solidifies leaving a polymer fiber.

Optimally labs use a high accuracy, ultra smooth syringe pump todeliver a constant stream to pulled spray nozzle. A driving electrodeis placed into the melt at the nozzle top and another electrode isused as a ground inside of the collector, typically a wire mesh. A highvoltage (30kV) is passed through the driving electrode, which createsan electric field on the surface of the liquid. The strength of the

Polymersolution

Jet

PipetteTaylor cone

High Voltage Supply

Syringe Pump

Collector Screen(Rotating or Stationary)

electric field is exactly opposite that of the surface tension of theliquid, creating a further increase in the intensity of the existingelectric field. The surface of the liquid changes into a hemisphereand elongates into a conical shaped called the Taylor cone(reference, figure 3).

Once the electrostatic force overcomes the surface tension acharged steam of fluid is ejected from the Taylor cone, the exactvalue is considered the critical value. The stream is collected at theground and once it dries forms polymer fibers. The process iscontrolled through speed of the fluid from the syringe pump and themagnitude of the electric field.

Variables:

1. Molecular Weight, Molecular-Weight Distribution and

Architecture

2. Solution Properties: Viscosity, Conductivity,

Surface Tension

3. Electric Potential, Flow Rate, Collection Screen

4. Distance between capillary and collection screen

5. Ambient Parameters: temperature, humidity,

chamber air velocity

6. Motion of the Target Screen (collector) and chamber

Air Velocity

Figure 3: Photograph of a meniscus of polyvinylalcohol in aqueous solution showing a fibre beingelectrospun from a Taylor cone.

Figure 2: (A) Vessel Scaffold, (B) Scaffold for Muscle, (C) Tissue Scaffold, (D) Bone Scaffold, (E) Organ Scaffolds.

A B C D E

Figure 1: Schematic of the Electrospinning setup.

Order # Product

RM1 70-3007 PHD ULTRA™ Programmable Syringe Pump

KEY FEATURESKey Regulator Certifications

Continuous smooth flow without droplet formation

Fully programmable

Ideal for optimization protocols

High Accuracy (+/-0.25%) low flows

Ideal for high viscosity solutions

Rugged design with 2 year warranty


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PHD ULTRA™ Push-Pull Continuous Flow Syringe PumpContinuous Flow for Cell Reactor Mainframe

The PHD ULTRA™ Push-Pull syringe pump provides virtually pulsefree, high accuracy and high precision flow to any bioreactor system.Complex programs can be written using on-board EZ Pro software toreduce shear stress during cell deposition and adhesion during tissueand organ development. Conversely, the same complex programscan be used to create particular shear forces on developing cells.

The PHD ULTRA™ Push Pull combines the high accuracy of ourULTRA line of pumps. Harvard Appatatus also has a wide array oftemperature control products to provide complete environmentalcontrol. Our 2 year warranty and robust constuction allow thesepumps to work continuously for extended periods of time withunsurpassed reliability.

Figure 1: Pressure-driven flow was continous using a programmable push-pull syringe pump(Harvard Apparatus, Holliston, MA). Media was equilibriated with 10% or 21% O2 in a gasexchanger made with gas-permeable silastic tubing.

KEY FEATURESCompensating Flows:

- The control of continuous infusion and

simultaneous withdrawal of liquids while

monitoring fluid levels

Perfusion Across Tissue Beds:

- Directional control of flows across tissue beds using

switching valves

Continuous Flow with High Accuracy and Smooth Flow:

- Pump Any Volume Large or Small with Smooth,

Non Pulsating Flow

Continuous Accurate Flow for High Pressure Systems:

- Unlike peristaltic pumps, syringe pumps can pump

against high pressures

Easily Sterilized Flow Path:

- By replacing syringe and tubing with a sterilized

set, this pump can maintain it’s sterility

Order # Product

RM1 70-3009 PHD ULTRA™ Push-Pull Programmable Syringe Pump

Watch a video demonstation of a PHD ULTRA™ Push-Pull Programmable Syringe Pump.To view press release, use your smart phone to scan the QR code or to view in a web browser go to http://tinyurl.com/44to6pv

References:

1. High-density cell seeding of myocyte cells for cardiac tissue engineering, Radisic et.al., 2008, Biotechnology and Bioengineering, Volume 82, Issue 4

2. Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydogels,Tsang et. al., 2007, FASEB, Volume 21

3. Formation of Steady-State Oxygen Gradients In Vitro, Application to Liver ZonationAllen et. al., 2003, Biotechnology and Bioengineering, Volume 82, No 3



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Fast Bolus Injector for Cell Therapy

Figure 1: Bone Marrow aspiration or Infusion Needles

Figure 2: Bone Marrow Injection Sites. Figure 3: Catheter placed intoright hepatic vein.

KEY FEATURESInject a fast bolus of large or small volumes in just

one second; up to 12X faster than standard pumps

The highest accuracy and precision available

Programmable with multiple methods

Easy-to-use icon interface

CE, ETL, CSA, UL and CB scheme Global compliance

Delivery from picoliter to hundreds of ml/min

For delivery of fastinfusion or bolusinjections into Liver,Kidney or Bone, theFast Bolus Injectormakes it easy. TheFast Bolus Injector canmount a 40 ml syringeor larger andaccurately inject 100 nl or 100 µl per injection with the push of a footpedal. This high linear force pump can pump up to 85 to 433 poundsof force easily, to inject viscous materials, cell suspensions or pastes.

Also a full selection of research surgical products are available: Bone aspiration and infusion needles, syringes, surgical equipments, anesthesia circuits, surgical drapes and sponges, ventilators capno-graphs and pulse oximeters, catheters, sutures, bone drill and more.

Bolus Delivery Programming is as easy as setting the amount to bedelivered and the time frame you want to deliver, i.e 5 µl in threeseconds.

Multiple methods can be stored and recalled via touch of a button,making the Fast Bolus Injector the ultimate, easy to use syringepump. Methods can be emailed and downloaded from your PC to thepump. Share a method with a colleague or duplicate a complexexperimental set-up with an e-mailed method.

References:

Human Bone Marrow–Derived Mesenchymal Stem Cells for Intravascular Delivery ofOncolytic Adenovirus 24-RGD to Human Gliomas, [Cancer Res 2009;69 (23):8932–40] .

Fast Bolus Injector for Cell Therapy Specifications

ACCURACY ±0.5%

REPRODUCIBILITY ±0.05

SYRINGE (MIN./MAX.) 0.5 µl / 140 ml

FLOW RATE: 6.00 mm

MINIMUM 20 pl/min using 0.5 µl syringe

MAXIMUM 2,000 ml/min using 140 ml syringe

DISPLAY 4.3" LCD Color Display with Touchpad Non-Volatile Memory Stores all settings

CONNECTORS:

LINEAR FORCE 34 kg (75 lbs) @ 100% force selection

# SYRINGES/PUMP 2,4,8,10 (1.4 Liters capacity)

PUSHER TRAVEL RATE:

MAXIMUM 0.18 µm/min

MINIMUM 190.80 mm/min

DIMENSIONS 10.16 x 30.48 x 21.59 cm (4 x 8.5 x 12 in) (H x W x D)

WEIGHT 4.5 kg (10 lbs)

REGULATORY CERTIFICATIONS CE, UL, CSA, CB Scheme, EU RoHS

Order # Product

RM1 98-4279 Fast Bolus Injector for Cell Therapy

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PHD ULTRA™

The PHD ULTRA™ is the solution for your most demanding fluidicsapplications. This pump represents the latest technology in syringepumps and was developed utilizing the feedback of the world’slargest population of syringe pump users.

The PHD ULTRA™ will change the way you think about syringepumps. There are three major reasons the PHD ULTRA™ is the newstandard for syringe pumps:

1. Patent pending mechanical drive mechanism and

syringe holding mechanics to achieve the highest

performance of any syringe pump.

2. EZ PRO Software and user interface allow easy

programming of Methods from simple to complex, all

without the use of a PC.

– Preprogrammed Methods for simple to complex operations that allow you to be up and running with the touch of a button.

– LCD, high resolution, color touch screen for powerful functionality, yet very easy to use.

3. Levels of Versatility

a. Configurations: Standard, push-pull, remote, high pressure, multi-racks.

b. Connectivity: For USB or RS-232 computer control; RS-485 or optional RJ-11 for daisy chain (control multiple pumps).

c. Orientation: Horizontal or vertical orientation to optimize bench space or to minimize tubing.

Since 1901 Harvard Apparatus has been supporting bioresearchfluidics requirements beginning with the introduction of the firstcommercial syringe pump for bioresearch in 1956. Since 1956, over70,000 satisfied syringe pump users around the world have madeHarvard Apparatus syringe pumps the world’s #1 choice.

The PHD ULTRA™ Syringe Pump series is a family of high-accuracy,easy to use, rugged pumps designed for versatile applicationsincluding mass spectrometry calibration, drug and nutritional studies,microdialysis, dispensing, chromatography, LC/HPLC and more.

KEY FEATURESNew patent pending drive mechanism for unmatched

smooth flow, accuracy and precision

For operation at pl/min to ml/min flow rates

Easily program simple to complex methods

without a PC

Alphanumeric keypad for easy Method

naming and recall

Real and relative time clocks

Intuitive touch screen and icon interface

Vertical or horizontal orientation

Adjustable linear force to 75 lbs

Multi-syringe racks for multi-channel operation or

large capacity reservoir

Legendary reliability– 2 year warranty

APPLICATIONSMicrofluidics

Drug/Nutritional Delivery

Electrospinning

Reaction Chamber Addition

Mass Spec Calibration

Feeding Cells

Low Pressure Chromatography

Continuous Flow

Flow Programming

Gradients

% Composition Step Changes

Large Flow Deliveries

I/O Interactive Experiments

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Advanced Features of the PHD ULTRA™ Family


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Advanced Features of the PHD ULTRA™ Family (continued)

Highest Accuracy and Precision

The PHD ULTRA™ syringe pump family has a new patent pendingfluidics drive mechanism which assures ease of use and highperformance for the smoothest, most accurate flow rates of anysyringe pump. Flow rates of 1.56 pl/min to 215.8 ml/min are accuratewithin 0.25% and reproducible within 0.05%. A microprocessor-controlled, small step angle stepper motor drives a lead screw andpusher block. Advanced micro-stepping techniques are employed tofurther reduce the step angle to eliminate flow pulsation.

Maximum Experimental Versatility

This pump can be oriented vertically or horizontally for optimumexperimental connectivity. This pump comes standard to hold 2syringes, but can be purchased with 3 other syringe racks: 6/10syringe rack, 4 x 140ml syringe rack and 4 x microliter syringe rack.Syringe racks can be ordered separately. Multi-syringe racks providemulti-channel operation or serve as a large capacity reservoir.

• The standard 2-syringe rack holds 2 syringes from 0.5 µl to 140 ml

• The 4 x 140 multi-rack holds four 60 ml or 140 ml plastic syringes only

• 6/10 multi-rack will hold up to 10 syringes from 0.5 µl to 20 ml andup to 6 syringes from 30 ml to 60 ml

• The microliter syringe rack independently holds 4 syringes, from 0.5µl to 10 ml, enabling syringes of different sizes to run simultaneously.

Easy-to-Use Interface

The PHD ULTRA™ Syringe Pumps are very easy to use with an LCDcolor touch screen and icon interface. The Message Area of thetouch screen is used to display helpful instructions for the currentlydisplayed screen. It is also used to display error or warningmessages to indicate problem conditions in a Method or errorconditions during pump operation. The Run Screen shows all of thepump parameters on one screen for easy review.

Pump Models

The PHD ULTRA™ Pumps are available in three configurationsdesigned for different operating environments and varying degreesof operational flexibility.

Infusion Only: This model supports infusion operations at user-defined flow rates and with selectable target volume or timevalues to control the total infusion volume. The Infusion Onlymodels do not include programmable, user-defined Methods.

Infusion/Withdrawal: This model supports infuse only,withdraw only, infuse/withdraw and withdraw/infuseoperations at user-defined flow rates and with selectabletarget volume or time. Users can create and store oneMethod up to 800 steps.

Infusion/Withdrawal Programmable: This model supportsinfuse only, withdraw only, infuse/withdraw andwithdraw/infuse operations. Users can create and storemultiple Methods of up to 800 steps on the pump.

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Program Description

To operate the PHD ULTRA™, the user defines all the requiredparameters for infusing and/or withdrawing liquids through aMethod. This may be a Quick Start, Pre-Programmed or User-Defined Method. The basic operation is a simple 3-step procedure:

1. Select a Method

2. Enter operating parameters

3. Preview or Run your Method

Quick Start Methods are for simple infusions, withdrawals or acombination (depending on the pump model). Custom user-definedMethods can be created when a more advanced Method is required.The setup for a custom Method is easy using the standard profilesfound on all Infusion/Withdrawal and Infusion/WithdrawalProgrammable PHD ULTRA’s. The list of available profiles are:

By programming and saving custom Methods in the pump, multi-user errors are reduced. Easily transfer complex methods to otherpumps and/or download methods from a PC. Forget having toduplicate method-development efforts for each new pump added toyour system.

In addition to the advanced pumping profiles listed above, the PHD ULTRA™ contains a variety of advanced options allowing the userto repeat steps, link methods, control valves, external triggers etc.

Advanced Programming Features

Flow Programming: Change the flow with time, volume or atriggered event as many times as you like.

Bolus: Inject a large volume of drug (or drugs) at once. The bolus injection can be made in time or volume.

Concentration Delivery: Calibrate flow in concentration units ofmg/kg easily so flow is calibrated to concentration of drug andanimal weight.

Gradients: EZ PRO software allows you to easily programgradients, continuous or stepped.

% Ratio: Up to three solvents.

I/O: Dedicated and user defined I/O.

Pulsed Flow: So you can program the pulse easily.

Advanced Connectivity

All PHD ULTRA™ Syringe Pumps come standard with a footswitch,USB, RS-232, RS-485 and I/O connectors. There is also an option forRJ-11 connectors and analog control. These options have to beordered at the time the pump is ordered.

Constant Rate Bolus Pulse

Ramp Concentration Autofill

Stepped Gradient

FOOTSWITCH INPUT Start and stop a pump

USB AND RS-232 SERIAL INPUTS

Control your pump with a computer

RS-485 CONNECTORS Connect multiple pumps together (up to 99)

Connect remote mechanism

Connect satellite pumps to the Master pump forbinary or ternary gradient system (% composition)

RJ-11 CONNECTORS(OPTION)

Connect multiple pumps together (daisy chain)

USER I/O CONNECTOR

Direction Control input

Set pump to infuse or withdraw

Trigger Input Connect and external device to start or stop a pumpor Method

Footswitch Input Start and stop a pump

Trigger 1 Ouput Signal another device to start and stop a pump or Method

Trigger 2 Output Signal another device to start and stop a pump or Method

Sync Output Synchronize other devices

Valve Output External valve control

Run Indicator Connect an external LED or monitoring device to a pump

ANALOG CONTROL(OPTIONAL)

Analog control of the motor speed (0 to 10 v). This optionmust be ordered at the same time the pump is ordered.

USBSERIAL INPUT(for communication

from PC)

FOOTSWITCHINPUT

(switch sold separately)

USERI/O CONNECTOR

RS-485CONNECTORS(for pump-to-pump

communication)

ANALOGCONTROL CONNECTOR (optional)

RS-232 SERIALCONNECTOR

(for communicationfrom PC)

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PHD ULTRA™ Specifications

TYPE Microprocessor multiple syringe, infusion only, infusion/withdrawal or infusion/withdrawal programmable

ACCURACY ±0.25%

REPRODUCIBILITY ±0.05%

SYRINGES:

TYPE Plastic, glass or stainless steel

SIZE MINIMUM 0.5 µl

SIZE MAXIMUM 140 ml

FLOW RATE:

MINIMUM 1.56 pl/min

MAXIMUM 215.8 ml/min

DISPLAY 4.3" WQVGA TFT color display with touchscreen

CONNECTORS:

RS-232 9 pin D-Sub Connector

RS-485 6-position IEEE-1394

USB Type B

I/O & TTL 15 pin D-Sub connector

FOOTSWITCH Phono jack

LINEAR FORCE 34 kg (75 lbs) @ 100% force selection

STEP REVOLUTION 0.082 µm/step

VOLTAGE RANGE Universal input 100/240 VAC, 50/60 Hz

DIMENSIONS, H x W x D 10.16 x 30.48 x 21.59 cm (4 x 12 x 8.5 in)

WEIGHT 4.5 kg (10 lbs)

REGULATORY CERTIFICATIONS CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme


Order # Product

PHD ULTRA™ Options

RM1 70-3030 RS-232 RJ-11 Connectors Option (daisy chain)

RM1 70-3033 Analog Control Input Option (0-10V)3

RM1 70-3031 Internal Pinch Valve Option2

RM1 70-3032 Internal 3-Way Isolation Valve Option2

RM1 70-3034 Internal Fan Option4

PHD ULTRA™ Syringe Rack Kits and Upgrades

RM1 70-3020 6/10 Multi-Syringe Rack for PHD ULTRA™

RM1 70-3021 4x140 Multi-Syringe Rack for PHD ULTRA™

RM1 70-3022 Microliter Rack, for PHD ULTRA™ holds 4 syringes

RM1 70-3023 Anti-Siphon Kit for PHD ULTRA™

RM1 70-4010 Upgrade Infusion Only to I/W1

RM1 70-4011 Upgrade Infusion Only to Programmable1

RM1 70-4012 Upgrade I/W to Programmable1

1 Note: Requires Return to Factory2 Note: Only for available for Infusion/Withdrawal or Programmable Models3 Note: Only for Programmable Models. Not available on Satellite Boxes.4 Note: Fan option is required if external operating ambient is expected to be >35˚C

Accessories

RM1 70-4000 RS-485 Cable for Pump-to-Pump Communication, 0.5 m (1.6 ft)

RM1 70-4001 RS-485 Cable for Pump-to-Pump Communication, 2 m (6.6 ft)

RM1 70-4020 RS-485 Extension Cable, 9.1 m (30 ft)

RM1 70-4002 USB Cable for PC-to-Pump Communication, 2 m (6.6 ft)

RM1 70-4003 USB Cable for PC-to-Pump Communication, 5 m (16.4 ft)

RM1 70-4004 RS-232 Cable for PC-to-Pump Communication, 9 pin D-sub, 2 m (6.6 ft)

RM1 70-4005 Adapter, PHD Digital I/O

RM1 70-4006 Adapter, D-sub 15 to Term. Blk

RM1 72-8340 Adapter, USB to Serial

RM1 70-2215 Footswitch (with Phono Plug)

RM1 55-7002 Auto Fill Valve Box, Normal Pressure, 30 psi

RM1 55-7008 Auto Fill Valve Box, High Pressure, 200 psi

RM1 55-7760 Cable Assy, Daisy-chain, Legacy RS-232 RJ-11, 0.6 m (2 ft)

RM1 72-2478 Cable Assy, Daisy-chain, Legacy RS-232 RJ-11, 2.1 m (7 ft)

RM1 55-8000 Adapter for 25 ml, 50 ml, 100 ml Hamilton GasTight™ Syringes

Product Order # Order # Order # Order #

PHD ULTRA™

Standard Stand Alone Remote Satellite* Syringe Pump Module

PHD ULTRA™

Infusion Only RM1 70-3005 RM1 70-3305 – –

PHD ULTRA™

Infusion/Withdrawal RM1 70-3006 RM1 70-3306 RM1 70-3406 RM1 70-3506

PHD ULTRA™

Infusion/Withdrawal Programmable

RM1 70-3007 RM1 70-3307 – –

PHD ULTRA™

Push/Pull Stand Alone Remote Satellite* Syringe Pump Module

PHD ULTRA™

Push/Pull Infusion/Withdrawl

RM1 70-3008 RM1 70-3308 RM1 70-3408 RM1 70-3508

PHD ULTRA™

Push/Pull Infusion/WithdrawlProgrammable

RM1 70-3009 RM1 70-3309 RM1 70-3410 –

PHD ULTRA™

High Pressure Stand Alone Remote Satellite* Syringe Pump Module

PHD ULTRA™

4400 Pump I/WProgrammable

RM1 70-3010 RM1 70-3110 RM1 70-3410 RM1 70-3510

PHD ULTRA™

Hpsi Remote Pump I/W Programmable

– RM1 70-3111 – –

PHD ULTRA™

Hpsi Remote Pump I/W Programmablewith 10 x 140 Rack

– RM1 70-3112 – –


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Pulsatile Blood PumpsFor minimal cell damage delivering physiological flow

If you are performing cardiovascular work, this is the pump for you.It truly simulates the pumping action of the heart. It features siliconerubber-covered heart-type ball valves and smooth flow paths whichminimize hemolysis. Only inert materials like silicone rubber, acrylicplastic, and Teflon contact the fluid. The pumping head is easy totake apart and reassemble and can be sterilized.

Outstanding Performance

The pulsatile output closely simulates the ventricular action of theheart. This action provides physiological advantages in blood flow forperfusion in cardiovascular and haemodynamic studies. It is ideal forisolated organ perfusion, whole body perfusion, blood transfers,hydration/dehydration procedures, and blood cellular profile studies.

Pump Mechanism

A positive piston actuator and ball check valves provide theproportioning action. The product of stroke rate times stroke volumeis an accurate indicator of the flow rate. Positive piston actionprevents changes in flow rates, regardless of variations in resistanceor back pressure. The piston always travels to the end of theejection stroke, independent of the volume pumped. The Pumpcompletely empties at each cycle.

Specials - A special motor control module can be used for external 0-10 VDC control for those customers that wish to control with acomputer or other device.

Pulsatile Blood Pump Output

Acrylic Nozzle

Polished AcrylicPiston

TransparentAcrylic Cylinder

Fluoro Elastomer“O” Rings

AluminumCoupling Nut

Input

SiliconeBall-CheckValve

Acrylic ChamberTransparent

SiliconeBall-CheckValve

KEY FEATURESPulsatile output truly simulates the ventricular

action of the heart

Minimal hemolysis

Models for mice to large animals

Ideal for moving emulsions, suspensions, and

Non-Newtonian fluids such as blood

Harvard Apparatus Pulsatile Blood Pump Specifications

Mice/Rats Rabbits Dogs/Monkeys Large Animals; Hemodynamic Studies

STROKE VOLUME,ADJUSTABLE

0.05 to 1 ml 0.05 to 10 ml 4 to 30 ml 15 to 100 ml

RATE, STROKE/MIN. 20 to 200 20 to 200 20 to 200 10 to 100

MINUTE VOLUME, STROKE VOLUME x RATE

1 to 200 ml 10 to 2,000 ml 80 to 6,000 ml 150 to 10,000 ml

PHASING Fixed Phase Fixed Phase Adjustable Phase Adjustable Phase

SYSTOLE/DIASTOLE RATIO 35% Systole, 65% Diastole 35% Systole, 65% Diastole 35% Systole, 50% of total cycle 35% Systole, 50% of total cycle

TUBE ID 8 mm (.31 in) 8 mm (.31 in) 13 mm (.5 in) 15.9 mm (.625 in)

DIMENSIONS, H x W x D 312 x 156 x 250 mm(12.5 x 6.25 x 10 in)

312 x 156 x 250 mm(12.5 x 6.25 x 10 in)

500 x 212 x 337 mm(20 x 8.5 x 13.5 in)

500 x 212 x 337 mm(20 x 8.5 x 13.5 in)

WEIGHT 7.3 kg (16 lbs) 7.3 kg (16 lbs) 13.6 kg (30 lbs) 14.5 kg (32 lbs)

VOLTAGE 115/230 VAC, 50/60 Hz 115/230 VAC, 50/60 Hz 115 VAC, 60 Hz 230 VAC, 50 Hz 115 VAC, 60 Hz 230 VAC, 50 Hz

Order # RM1 52-9552 RM1 55-1838 RM1 55-3321 RM1 55-3339 RM1 55-3305 RM1 55-3313



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Pulsatile Blood Pumps (continued)

Pressure and Flow Curves Using Harvard Pulsatile Blood Pump forDogs/Monkeys in Isolated Perfusion of Left Lower Lobe of Dog Lung*

Pa Pulmonary Artery PressurePv Pulmonary Venous PressureQpa Pulmonary Artery Blood Flow

Instrumentation:

Pressure Statham

Flow Biotronex Electromagnetic Flowmeter

Recording Electronics for Medicine*Note: The above data is supplied through the courtesy of Cardiorespiratory laboratory Columbia-Presbyterian Medical Center New York, New York, Dr. Alfred P. Fishman, Director.

Figure 1: A Novel Culture System Shows that Stem Cells Can be Grown in 3D and UnderPhysiologic Pulsatile Conditions for Tissue Engineeringof Vascular Grafts.

References

Abilez, et al Journal of Surgical Research 132, 170–178 (2006)doi:10.1016/j.jss.2006.02.017.

Pressure Curves

The shape of the output pressure curve is a function of both thepump action and the characteristics of the external system on theoutput valve side. The following set of curves were obtained withModel 1421, using water as the pumped medium. In the tests,“Sanborn” pressure transducers were inserted in three places andcontinuous records obtained undery varying conditions.

Curve A Pressure just beyond the output valve

Curve B Pressure within the pump chamber

Curve C Pressure just before the intake valveBy variation of parameters involved (peripheral resistance, strokerate, stroke volume and phase ratio), an infinite number of outputflow characteristics can be obtained.

Hemolysis Test Conditions Models forDog/Monkey (RM1 55-5321) and Large Animals(RM1 55-3305)

Hemolysis ranged from 0.114 mg% to 0.29 mg% per pass throughthe various pumps, with an error of ±10%.

To put these results in perspective, most physiological perfusionsare run with flow rates and total blood primes, such that the numberof passes through the pump will range from about 1/4 to 3/4 perminute. Assuming one pass in two minutes and no physiologicalremoval of the products of hemolysis, then hemolysis rates wouldrange from 3.4 mg% to 8.7 mg% per hour of pump use.

In these studies a reservoir of 500-800 cc of fresh dog blood wasused, connected to the pump by 3/8” PVC tubing. Samples at roomtemperature were taken at 15 and 30 minute intervals for 4 to 5hours. Samples were spun down and hemolysis measuredimmediately using the method of Flink and Watson. Since the rate ofhemolysis depends on the amount of blood in the system and theflow rate, the results are reported as mg% per pass. The flow ratedivided by the volume of blood in the system determines thenumber of passes through the pump per minute.



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Centrifugal Pump for BloodVirtually eliminates cell lysis

The centrifugal pump is specifically designed for pumping bloodand/or erythrocyte suspension solutions in the physiological orpharmacological laboratory. It consists of the pump drive BVP-ZX anda centrifugal pump head which can be replaced without tools. ThePump Drive and Pump Head must be purchased separately. Pumpheads are hermetically sealed. The coupling to the motor of thepump drive is carried out via magnetic force; there is no axle.

The pump speed is set using a 3-digit potentiometer switch (000 to999) or via an analog interface.

The drive is very robust and suitable for continuous speed selectionoperation.

Centrifugal Pump

KEY FEATURESLow hemolysis

Flow rates up to 16 L/min

Little to no pulsation

Smooth run, producing only low noise

Pump heads interchangeable without tool

Speed setting by a digital switch in 0.1% steps

“Max Speed” button for quick fill or ventilate

Robust construction for long life

Analog interface for remote control

Centrifugal Pump for Blood Specifications

PUMP DRIVE:

TYPE BVP-ZX

SPEED 3 to 3000 rpm, adjustable in 0.1% steps

MAINS CONNECTION 230 (50/60 Hz) 115 V (50/60 Hz)

POWER CONSUMPTION 120 W maximum

ANALOG INTERFACE Speed control 0–5 V or 0–10 V or 0–20 mA or 4–20 mA,start/stop (TTL contacts)

PROTECTION RATING IP 30

OPERATING CONDITIONS 0˚ to 40˚C (normal environmental conditions)

DIMENSIONS, H x W x D 260 x 155 x 260 mm (10.2 x 6.1 x 10.2 in) without pump head

WEIGHT 7 kg (15.4 lbs) without pump head

CENTRIFUGAL PUMP HEADS:

TYPE BP-80 SP-45

MANUFACTURER Medtronic Terumo

PUMP TECHNOLOGIES Centrifugal Impeller (Centrifugal)

MAXIMUM FLOW RATE 10 l/min at 50 mmHg – 16 l/min at 50 mmHg3 l/min at 300 mmHg – 13 l/min at 300 mmHg

PULSATION No Yes

PRIMING VOLUME 80 ml 45 ml

INLET/OUTLET ID 9.5 mm 9.5 mm

PULSATION Direct Adapter SP-03 Required

Order # Product

RM1 73-2963 BVP-ZX Centrifugal Pump Drive 115 VAC

RM1 73-2470 BVP-ZX Centrifugal Pump Drive 230 VAC

RM1 73-2807 BP-80 Centrifugal Pump Head

RM1 73-2955 SP-45 Centrifugal Pump Head

RM1 73-2956 SP-03 Adaptor for SP-45 Head


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RM1 73-3296

REGLO Digital/Analog Programmable Peristaltic Pumps

REGLO Digital 2 channel, 6, 8 or 12 Roller Pumps

Tube-bed with 2 snap-on MS/CA Click ‘n’ Go Cassettes included for 3-stop collared tubing. See our website.

REGLO Digital 4 channel, 6, 8 or 12 Roller Pumps

Tube-bed with 4 snap-on MS/CA Click ‘n’ Go Cassettes included for 3-stop collared tubing. See our website.

KEY FEATURESDispensing mode flow rates from 0.002 to 68 ml/min

Snap-on MS/CA click ‘n’ go cassette included

RS-232 interface

Adjust and calibrate dispensing volumes in ml

and flow rates in ml/min for accurate and

reproducible results

Easy to use with pre-programmed flow rates for

all available tube sizes

Dispensing by volume, time or intervals for each

unique application

Overload protection and indicator automatically

stops pump to prevent damage

Display readout: speed in 1% steps and flow rate

in ml/min

Motor and ventilation permit 24 hour continuous

operation

Ease of use and a clear function display are the special

features of these small pumps. They offer versatile

dispensing functions, provide reproducible, accurate

results and take up very little bench-space.

REGLO Specifications

REGLO DIGITAL 4 CHANNEL:

MS-4/8-100

CHANNELS 4

PUMP ROLLERS 8

FLOW RATES, MINIMUM 0.002 ml/min

FLOW RATES, MAXIMUM 35 ml/min

SPEED RANGE 1 to 100 rpm

MAINS CONNECTION 115 V / 50 Hz or 230 V /50 Hz

POWER CONSUMPTION 20 W

REVERSIBLE FLOW yes

SET FLOW digital, 3–4 digits according to function (mode), LED display

RS-232 INTERFACE for control of all functions

DISPLAY INPUT (TTL LEVEL) Run/Stop, AutoStart

BACK PRESSURE, MAX. 1.0 bar (14.5 PSI)

SUCTION HEIGHT 7–8 m

PROTECTION RATING IP 30

TUBING CASSETTE MS/CA Click ‘n’ Go Cassettes are included

DIMENSIONS, H x W x D 135 x 100 x 190 mm (5.3 x 3.9 x 7.5 in)

WEIGHT 2.1 kg (4.6 lbs)

Order # Product

RM1 73-3054 Replacement MS/CA Click ‘n’ Go Cassette

RM1 73-3055 MS/CA Pressure Lever Cassette

RM1 73-3050 Foot Switch for Reglo Digital Pumps

RM1 73-2950 REGLO Digital 4 Channel, 115 VAC, 60 Hz, 4 Ch/6 Roller






RM1 73-0113 REGLO Analog, 115 VAC, 50 Hz, 4 Ch/8 Roller

RM1 73-0114 REGLO Analog, 230 VAC, 50 Hz, 4 Ch/8 Roller

Digital Control Panel Analog Control Panel


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MCP Pump Drive

This programmable pump drive offers various dispensing modes,providing highly reproducible and accurate results. Pump head mustbe purchased separately. A wide selection of pump heads withsingle or multi-channel capabilities are available for the MCP pumpdrive. See pages 30 to 32.

Multi-functional Display

For tube sizes, speed, flow-rates, dispensing time, dispensingvolume, interval duration and totally delivered volume as well asoperational mode, flow direction and MAX-key for priming.

Calibration: Dispensing Volume/Flow-rate

Volume and flow rate can be pre-set and calibrated in ml or ml/min.The drives are pre-calibrated according to the pre-programmed pumpheads and tube sizes.

Application

Filling of bottles and test tubes as well as dispensing of pre-definedvolumes.

1

2

3

4

5

6

7

8

Rear panel of MCP

1. Foot Switch/Hand Dispenser

2. RS-232 Interface (in)

3. RS-232 Interface (out)

4. Valve Connector

5. Analog Interface

6. Voltage Selector

7. Fuse Holder

8. Mains Supply Socket

All functions of the MCP drive can be controlled via PC via the RS-232 interface

KEY FEATURESMicroprocessor controlled, digital drive stores

4 programs in memory

Flow-rates of the pump-heads are pre-programmed

according to the different standard tubing sizes

Dispensing volumes in ml and flow rates in ml/min

Calibrate in ml/min

Various dispensing modes (time, volume, interval and

flow rate, drip-free)

MAX-key for priming and rapid filling or emptying of

the tube system.

MCP Pump Specifications

MODEL MCP pump drive only, pump head must be purchased separately

SPEED 1 to 240 rpm with 0.1 rpm resolution

BACK PRESSURE 1.5 bar maximum (22 PSI)

MAINS CONNECTION 35 ml/min

SPEED RANGE 1 to 100 rpm

MAINS CONNECTION 115 V (50/60 Hz) or 230 V (50/60 Hz)

POWER CONSUMPTION 100 W maximum

RS-232 INTERFACE Baud rate 9600 or 1200 baud, 8 bit, 1 stop bit, no parity for complete computer control for cascade control or up to 8 pumps

ANALOG INTERFACE Speed control 0–5 V or 0–10 V respectively 0–20 mA or 4–20 mA

DISPLAY INPUT (TTL LEVEL) Flow direction, start/stop, speed control

VALVE PLUG 1 for 24 V valve

Order # Product

RM1 73-3026 MCP Pump Drive, 230 VAC

RM1 73-3029 MCP Pump Drive, 115 VAC

RM1 73-3048 Foot Switch for MCP Pump Drive


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Pump Head 380AD for Biologicals, Gentle PeristalticFits MCP and BVP pump drives

Single-Channel Pump Head 380AD

Pump Head 380AD Specifications

PUMP HEAD 380AD

CHANNELS 1

PUMP ROLLERS 3

FLOW RATES 0.41 to 3600 ml/min

BACK PRESSURE 1.5 bar (22 PSI) maximum with 1.6 mm wall thickness tubing2.5 bar (36 PSI) maximum with 2.4 mm wall thickness tubing

TUBING TYPE Standard Tubing

TUBING ID 0.8 to 11.1 mm; 4.8 to 6.4 mm

TUBING WALL THICKNESS 1.6 mm or 2.4 mm

Flow Rates for 380AD Pump Head Using

Standard Tubing

Tubing ID Wall Thickness Flow Rates, ml/minMin. Max.

0.8 mm 1.6 mm – –

1.6 mm 1.6 mm 0.41 99

3.2 mm 1.6 mm 1.5 370

4.8 mm 1.6 mm 3.4 830

6.4 mm 1.6 mm 6.2 1500

8.0 mm 1.6 mm 9.5 2300

9.5 mm 1.6 mm 13 3000

11.1 mm 1.6 mm 15 3600

4.8 mm 2.4 mm 3.4 830

6.4 mm 2.4 mm 6.2 1500

Order # Product

RM1 73-3035 380AD Single-Channel Pump Head for MCP/BVP Pump Drives

KEY FEATURESInstalls rapidly

Easily interchanged with other MCP/BVP pump heads

Ideal for chemical, biotechnological and pharmaceutical

applications

Suitable for viscous fluids and fluids containing a high

content of sensitive solids

Applications requiring hygienic conditions, durability

and reliability

This pump head features 3 convex rollers revolving in a concave tubebed which allows cells or particles to escape through a gap towardstubing wall to minimize damage. It is ideal for inoculating orharvesting mammalian cells.

Adjustable Pump Rollers

On this pump head, the 3 convex rollers can be adjusted and pressedsymmetrically against the concave tube bed, enabling the use ofpump tubing with various wall thicknesses.

Please note:

The listed flow rates are approximate values in ml/min. They havebeen determined under the following conditions:

• Medium: water

• Temperature: 22˚C

• No back-pressure

• Pump tubing: Tygon®

The exact flow rate depends on a number of parameters includingquality and age of the tubing, pressure onto the tube bed (adjustable),back-pressure, viscosity of fluid, temperature, etc.

Adjustable roller pressure accomodates wide range of tubingdurometers (stiffness).

Very simple tube loading. This pump head accepts tubing withdifferent diameters and wall thicknesses with ease. Thanks to theadjustable pump rollers, this is an ideal pump head for media withhigh viscosity or with a certain level of solid content.


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SB Pump Head for Standard ApplicationsFits MCP and BVP pump drives

SB Pump Head with 2V Tube Bed Set

2V

SB Pump Head with 3V Tube Bed Set

KEY FEATURESIdeal for sensitive substances requiring a smooth

pressure adjustment

Uses spring-loaded tube bed set –

choice of 1, 2 or 3 channels

Tube bed sets are interchangeable

0.009 to 1100 ml/min flow rates depending

on tube bed set

6 rollers

Individual and continuously adjustable pressure

setting per channel

Back pressure has maximum of 1.5 bar (22 PSI)

SB Pump Head Specifications

SB PUMP HEAD WITH 2V TUBE BED SET

PUMP HEAD SB

TUBE BED SET 2V

CHANNELS 1 – 2

PUMP ROLLERS 6


BACK PRESSURE 1.5 bar (22 psi) maximum

TUBING TYPE Standard tubing

TUBING ID 3.2 to 8.0 mm

TUBING WALL THICKNESS 1.6 mm

SB PUMP HEAD WITH 3V TUBE BED SET

PUMP HEAD SB

TUBE BED SET 3V

CHANNELS 1 – 2

PUMP ROLLERS 6


BACK PRESSURE 1.5 bar (22 psi) maximum

TUBING TYPE Standard tubing

TUBING ID 0.8 to 6.4 mm

TUBING WALL THICKNESS 1.6 mm

Flow Rates for SB Pump Head using Standard Tubing,

1.6 mm Wall Thickness

Standard 2V Tubing Bed Set 3V Tubing Bed Set

TubingID mm

ml/min ml/minmin max

ml/min ml/minmin max

0.8 – – 0.09 22

1.6 – – 0.26 63

3.2 1.1 260 0.99 240

4.8 2.3 550 2.2 530

6.4 3.7 890 3.6 870

8.0 4.6 1100 – –

Note: For BVP-Standard drive, the min. flow rate values must be multiplied by factor 2.4

Order # Product

RM1 73-3040 SB Pump Head for BVP/MCP Pump Drives*

RM1 73-3045 2V Tube Bed Set for SB Pump Head

RM1 73-3046 3V Tube Bed Set for SB Pump Head

*Note: Requires selection of Tube Bed Set.

3V



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Gentle Pumping Pump Heads for MCP & BVP Pump DrivesFor minimal to no cell damage

These pump heads provide gentle pumping action and are suitablefor many applications, including cell suspensions.

The model Pro-280 pump head is gentle enough to use for highlyviscous liquids with concentrated viable cells. Comparisons to gear,piston and centrifugal pumps proved that peristaltic pumps are theonly suitable and sterilizable pump system for gently pumping mediacontaining living cells.

RM1 73-3119 RM1 73-3120

Gentle Pumping Pump Heads Specifications

PUMP HEAD PRO-280 PRO-380 PRO-281 PRO-381

NUMBER OF CHANNELS 1 1 1 1

NUMBER OF ROLLERS 2 3 2 3

TUBING WALL THICKNESS 1.6 mm 1.6 mm 2.4 mm 2.4 mm

FLOW RATE RANGEML/MIN

0.49 - 3700 0.45 - 3400 3.6 - 3100 3.3 - 2900

Order # Product

RM1 73-3119 PRO-280 Gentle Pumping Head




Gentle Pumping Pump Heads Flow Rates

STANDARD TYGON® R-3603/R-3607

PRO-280 FLOW RATE, ml/minFLOW RATE, ml/min


Tubing ID mm Wall mm Min. Max. Min. Max.

1.6 1.6 0.49 120 0.45 110

3.2 1.6 1.9 450 1.7 400

4.8 1.6 4.2 1000 3.7 890

6.4 1.6 7.2 1700 6.5 1600

9.5 1.6 14 3300 13 3000

11.1 1.6 16 3700 14 3400

STANDARD TYGON® R-3603/R-3607



Tubing ID mm Wall mm Min. Max. Min. Max.

4.8 2.4 3.6 870 3.3 800

6.4 2.4 6.5 1600 5.8 1400

8 2.4 9.9 2400 8.8 2100

9.5 2.4 13 3100 12 2900

*Note: Approximate values: determined with water at 22˚C, no differential pressure, with Tygon® Tubing

KEY FEATURESUnique convex rollers cause minimal cell lysis

Ideal for mammalian cell inoculating, harvesting, or cell

suspension transfers

Elevated differential pressures (Pro-281 and Pro-381)

Suitable for viscous fluids and fluids containing a high

content of sensitive solids

Applications requiring hygienic conditions,

durability and reliability

Installs rapidly

Easily interchanged with other MCP/BVP pump heads

PRO-280Coated aluminum pump head

Can be dismantled for cleaning

Stainless steel rollers

Self-centering tube track allows tube to lie in the

optimum position, which considerably lengthens

the tube life

For applications which require hygienic conditions,

reliability and durability

Ideal for use in chemical, biotechnological and

pharmaceutical processes and in food industry

PRO-281Same as Pro-280 but:

For tubing with 2.4 mm wall thickness

Especially recommended for:

– Elevated differential pressures

– Viscous fluids

PRO-380Same as Pro-280 but

Less pulsation thanks to 3 rollersy

Slightly lower flow rate

PRO-381Same as Pro-380 but:

For tubing with 2.4 mm wall thickness

Especially recommended for:

– Elevated differential pressures

– Viscous fluids

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Perfusion AccessoriesMicro Valves & Micro Connectors

Manifold/Connectors, Needles & Tubing

Tubing, Connectors and Manifolds

Harvard Apparatus has a wide range of tubing, connectors andmanifolds so you can create the fluid pathway to meet your specificrequirements. We offer many types of tubing of different materialsand sizes. Our fluid manifolds start at 2 channels and go all the wayup to 24 channels. We have a multitude of connectors that allow youto complete the fluid connections and complete your system. Pleasecall our technical support staff for additional information.

Valves/Controllers (Fluids & Pressure) & Glass or Plastic Fluidic Chips

Until now, commercially available options for preparing microlitervolumes have suffered major drawbacks, including high backpressure, clogging, leakage and limited surface chemistry options.New CapTite cartridges offer customizable packing and re-use, foreasy sample preparation in both prototypes and high-throughput,automated systems.

In-Line PEEK cartridge for Toxic removal

KEY FEATURESFast

Cost-effective

Preparation of microfluidic volumes

Warner Electrophysiology & Cell Biology Catalog

For the electrophysiological, cellular, and neurological sciences. Ourextensive product line includes voltage and current clamp amplifiersfor whole-cell and patch applications. Bessel filters, chambers forimaging and recording systems, perfusion control systems andsteppers, solution heating systems, microscope translation tables,microelectrodes and holders, and glass capillary tubing, plus nowincludes electroporation and transfection systems from BTX.

Animal, Organ & Cell Physiology

This catalog features a broad range of products, including ourlegendary line of infusion and perfusion pumps, ventilatorsanesthesia systems, surgical instruments, equipment for small tolarger animals, and isolated organ and tissue systems for all levels ofresearch and education. These products are designed to help youachieve better research results in less time.

Request these Catalogs

Publication W2. Publication PY2.


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SWS-10, SWS-60 & SWS–140Syringe Warmers

SC-20 Dual In-line SolutionHeater/Cooler

Syringe warmer ona syringe pump

Syringe warmermounted on asupport stand

KEY FEATURESIndependent temperature control for individual syringes

Designed for use on a syringe pump or support stand

Dispensing volumes in ml and flow rates in ml/min

Accommodates 10, 60 and 140 cc syringes

Scale marking ports permit volume monitoring

during use

Can be powered from 12 volt battery for sensitive

electrophysiology applications

Order # Model Product

RM1 64-1584 SWS-10 Syringe Heater for 10 cc Syringes



RM1 64-1545 TC-124A Temperature Controller, 120 VAC US

RM1 64-1545E TC-124AE Temperature Controller, 240 VAC Europe

RM1 64-1655 TC-144 Temperature Controller

RM1 64-1606 BAC-1 Battery Adapter Cable

Syringe Warmer Specifications

HEATER RESISTANCE 18 ?VOLTAGE REQUIREMENT Variable to 12 V maximum

TEMPERATURE RANGE Ambient to 65°C

TEMPERATURE ACCURACY ±1°C

CABLE LENGTH 2.4 m

WARRANTY One year

MODEL WEIGHT LENGTH OD ID SYRINGE TYPE

SWS-10 32.7 g 38.2 mm 22.2 mm 16.2 mm Dickerson Becton

SWS-60 76 g 83.7 mm 35.0 mm 29.1 mm Dickerson Becton

SWS-140 192 g 109.5 mm 51.0 mm 41.4 mm Monoject


RM1 64-0353 SC-20 Solution Heater/Cooler Two Line

RM1 64-0352 CL-100 Bipolar Temperature Controller

RM1 64-0107 TA-29 Cable with Bead Thermistor for Heater Controllers

Heater/Cooler Specifications

MINIMUM TEMPERATURE 0°C (2 ml/min. max flow)

MAXIMUM TEMPERATURE 50°C

MAXIMUM FLOW RATE AT 5°C 5 ml/min.

ACCURACY ±0.1°C

INTERNAL DEAD VOLUME 330 µl

PERFUSION LINES Type 316 Stainless Steel, 0.032 in ID x 0.062 in OD

WATER JACKET PORTS Type 316 Stainless Steel, 0.12 in ID x 0.147 in OD

CONTROLLER Model CL-100 Bipolar Controller

PHYSICAL DIMENSIONS:

BODY (D xL) for control of all functions

WEIGHT Run/Stop, AutoStart

CABLE LENGTH 1.9 m

CONNECTOR TYPE 15 pin Male “D”

WARRANTY One Year

In-line solution heating has proven to be one of the most effectivemethods of maintaining the temperature of perfusion solutions. The SC-20 Dual In-line Solution Heater/Cooler utilizes Peltier thermo-electricdevices to regulate temperature both above and below ambient levels.

The SC-20 is designed to thermally regulate one or two solutions at thesame temperature. Solution temperature can be maintained at 0°C atflow rates of 2 ml/min., 5°C at 5 ml/min., or as high as 50°C at 5 ml/min.

An integral water jacket is used to remove excess heat from the SC-20peltier device. Running water either from a tap or a large reservoir canbe used. Flow rates as low as 4 liters per hour are sufficient to maintaincooling efficiency.

The SC-20 can be used with either one or two discrete perfusatesolutions or with a solution/gas combination. When coupled with a PHCSeries Imaging Chamber Heater/Cooler Jacket, the SC-20 provides aneffective means of temperature control in a Warner chamber, even inthe absence of solution flow.

Each SC-20 is supplied with a TA-29 Thermistor Cable Assembly formonitoring the bath temperature during use, 10 feet of PE-160 tubingand 10 feet of 1/8" I.D. x 1/4" O.D. Tygon tubing.

KEY FEATURESHeats and cools from 0° to 50°C

Compatible with Warner Series 20 Chambers

Optimized for use with the CL-100 Bipolar

Temperature Controller


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Syringe Pump Peristaltic Centrifugal Piston Pumps

Standard I/W Push Pull Rack Standard Gentle Head SSI Blood Pump

Application

Discrete Injection

Continuous

Volume To Be Delivered

Large

Small

Smooth

Smooth

Pusatile

Flow Rate

µl/min

ml/min

liter/min

# of Channels

Single

Multiple

Solution Content

Suspension/Cells

No Cells

Highly Viscous

Temperature Control

Cool

Hot

Other Features

Programmability

Accuracy

Sterilizable

Warranty (Years) 2 2 2 1 1 1 1 1

= Marginal = Good = Very Good = Excellent = N/A

Guide to Pump Capabilities



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Regenerative Medicine Pumping TechnologiesSyringe Pumps and Peristaltic Pumps

ADVANTAGESWorks at pressures up to 1500 psi

Highest precision < 0.1%

Pulse free flow

Accurately dispense very small to large volumes

Easily sterilizable

Can dispense or withdraw

Many easily programmable dispensing profiles: gradients

1 to 10 channels of operation

1.3 pl/min to 240 ml/min

Gentle on cells

DISADVANTAGESPumps finite volumes

Little more expensive

TYPICAL APPLICATIONAccurate dosing bioreactors

Accurate dispensing of drugs in animals

High pressure flow into reaction chamber

Syringe Pumps

A motor driven threaded rod (lead screw) slowly turns, moving theplunger of the syringe in, and pushing the fluid out. Reversing thedirection of the motor allows for withdrawal of fluids.

Peristaltic Pumps

Figure 1a-b: In this example, three rollers on rotating arms pinch thetube against an arc and push the fluid along. There are usually threeor four sets of rollers.

ADVANTAGESPumps continuous volumes

Sterilizable

Less expensive for multiple channel dispensing

1 to 16 channels of operation

Can dispense or withdraw

1 µl/min up to 13 l/min

Special gentle head for cells

DISADVANTAGESLow pressure operation 30 psi or less

Pulsing flow

Moderate precision 1-5%

Conventional hurt cells

TYPICAL APPLICATIONSPerfusion flow across tissue or cells

Pump in and out with balanced flow

Transfer liquids ie. controlled animal feeding

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Figure 1a Figure 1b


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Regenerative Medicine Pumping Technologies (continued)

Centrifugal Pumps, Reciprocating Piston Pumps and Diaphragm Pumps

Centrifigul Pumps

A centrifugal pump is a rotodynamic pump that uses a rotatingimpeller to create flow by the addition of energy to a fluid.

ADVANTAGESVery low maintenance

Can pump suspensions of particles or cells

Can pump aggressive/corrosive solutions

Self priming

Gentle on cells

DISADVANTAGESSingle channel operation


Can only dispense

TYPICAL APPLICATIONSPumping solution continually

Reciprocating Piston Pump

It is a pump with a piston that moves back and forth in a cavity andthe piston displaces fluid when it enters the chambers.

ADVANTAGESLow maintenance

Best for high pressure, high accuracy

Gentle on cells

Continuous flow


Moderate precision 1%

Can only dispense

TYPICAL APPLICATIONSHigh pressure high accuracy

Diaphragm Pumps

The brown rod shown in this drawing is moved by the pushrod. Itpushes the diaphragm in and a spring forces it back out.

ADVANTAGESVery low maintenance

Can pump suspensions of particles

Can pump aggressive/corrosive solutions

Self priming

Gentle on cells


Pulsing flow


Can only dispense

TYPICAL APPLICATIONSPumping highly corrosive materials

The Harvard ApparatusIntegrated Bioreactors

3D Organ BioreactorA 3D bioreactor is a factory with different parts working together to create a product. Inthis case, the product is a three dimensional organ. The 3D organ bioreactor has many keyelements which are tailored for each organ system produced.

3D

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34 FOR PRODUCT INFORMATION 508.893.8999 [email protected] www.harvardapparatus.com

Temperature MetabolicActivity

Mix

WasteManagement

Mechanical

3D Organ Bioreactor illustration used with permission courtesy of Dr. Paolo Macchiarini, 2011.


Advantage...for 3D Organ Generation

PROCESS CONTROLLER/ACQUISITION SYSTEM

Physiological

Metabolic

Biochemical

Environmental

Histological

Database Management

FLUID/GAS CONTROL SYSTEMS

Media

Buffer

Ventilator

Perfusion/Inject

SENSORSpH

O2

CO2

Glucose

Temperature

Cell Confluence

Vascular

Imaging (IRCI)

Weight

Pressure

Force

Flow

WASTE MANAGEMENTCO2

Lactate

Dialysis

Filtration

Solid Phase Extraction (SPE)

REACTOR VESSELBio-compatible materials

Perfusion/Injection/Electrical Portals

Heparin coating

Tissue Exercising

Imaging Capabilities

Sterilization SOP

Regulatory-GMP, others

Feedback Controlled Environment

Temperature Control

3D

Org

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Electrical

Nutrients

Respiration O2, CO2

Images

BIOREACTORSB

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InBreath 3D Organ Bioreactor,see page 38-39

Synthetic Scaffold Material forRegenerative Medicine,

see page 60

High Precision (25 microns)3-Axis Imaging Platform,see page 43


3D & multi-wellbioreactors

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Table of Contents

3D Organ Bioreactor 34-35

3D Bioreactor Poster fold out between 40-41

Regenerated Trachea Poster fold out between 40-41

InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels 37-38

Lung Bioreactor Poster fold out between 40-41

LB-2 Bioreactor for Mouse and Rat Lung Regeneration 39-40

Infrared Contrast Imaging Poster fold out between 40-41

KLB-2 Kidney and Liver Bioreactor 41

Linear Stress and Relaxation Bioreactor 42

3-Axis Imaging Platform Poster fold out between 44-45

High Precision 3-Axis Imaging Platform 43

Harvard Apparatus Accelerator 1000 Multi-Well 44-45

Imaging Bioreactor and Incubator 46

RC-37F and RC-367W 47

RC-37FC and RC-36WC 48Electric CO2 Microscope Stage Incubator 49

Electric CO2 Microscope Stage Incubator Accessories 50

Digital Gas Mixer 51-53

HPC-3, Hydrostatic Perfusion Chamber 54

C-Pace Cell Culture EP Stimulator 55-57

Stimulator C 58

Stimulator CS for Isolated Cells 59

Synthetic Scaffold Material 60-61

Lab Site Heparin Coating 62

Flow Through Device Accessories 63

BIOREACTORSB

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InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels

KEY FEATURESFacilitates cell seeding procedures on both sides of a

3D tubular matrix, ensuring homogeneous coverage

Proven Design - demonstrated regeneration of human

bronchus and successful human transplantation with

positive clinical outcome as recently published by

Macchiarini et al 2008

Allow seeding and culturing of different cell types on

either side of the tubular scaffold

Enhance oxygenation of the culture medium and mass

transport (oxygen, nutrients and catabolites) between

the medium and the adhering cells

Stimulates cells with hydrodynamic forces, favoring

metabolic activity and differentiation process

Allows the achievement and maintenance of sterility

and other criteria of Good Laboratory Practice (GLP),

simplicity and convenience

Permits the possibility of automation and scale-up/-out

InBreath is a rotating, double-chamber, bioreactor designed for cellseeding and culturing on both surfaces of a tubular matrix and includesrotational movement of the scaffold around its longitudinal axis. Apolymeric culture chamber houses the biologic sample and the mediumfor the whole culture period. Cylindrical scaffold holders are constructedwith working ends of different diameters - to house matrices of diversedimensions - and a central portion of smaller diameter to expose theluminal surface of the matrix for cell seeding and culturing. A co-axialconduit links the inner chamber to the external environment through aninterface at the chamber wall. This provides access to seed and feed theluminal surface of the construct. Secondary elements moving with thescaffold holder induce continuous mixing of the culture medium toincrease oxygenation and mass transport.

The bioreactor is made up of 3 components: the culture/constructchamber, a DC motor and a control unit. The DC motor turns thespindle in the culture chamber (0-5 rpm adjustable). The control unit isseparated from the DC motor by a cable. The control unit sits outsideof the incubator allowing researchers to change the speed of rotationwithout disturbing the incubator. This allows an optimal temperature tobe maintained for the cell/matrix construct.

Additionally, the culture chamber and DC motor are connected by asimple spindle. This allows the culture chamber to be removed fromthe motor as needed (i.e. for sampling, medium exchange, etc.). TheDC motor never leaves the incubator. Then the chamber can be placedback into the incubator quickly and easily.

The culture chamber is autoclavability, easy to handle under sterileconditions, reliability and precision ensure the full compatibility of thedevice with GLP rules.

PURPOSE BUILT REACTOR CHAMBERReactor container and spindle are made of

Polysulphone, Teflon and 316 Stainless Steel

allowing for:

- sterilization

- chemical inertness

- biological compatibility

- transparent for excellent visualization

Chamber has quick-fit spindle for easy removal of the

spindle and organ construct for analysis or dissambly

Rotating spindle assures even exposure to nutrient media

Integrated ports on spindle allow access to internal

(lumen) surface of organ

Compact dimensions allow placements of multiple units

within standard incubator

Offset cover allows for oxygenation of sample by non

sheering ambient air contact

Quick release and disassembly of parts makes

sterilization easy

EASY-TO-USE CONTROLLERCompact design for remote placement

(i.e. outside the incubator)

The rotational speed can be controlled from

0 to 5 rotations per minute

Motor overload indicator for safe operation

CE certified

References:

1. Clinical transplantation of a tissue-engineered airway, Macchiarini et al 2008, The Lancet, Volume 372, Issue 9655.

2. A double-chamber rotating bioreactor for the development of tissue-engineeredhollow organs: From concept to clinical trial, Asnaghi et al 2009, Biomaterials,Volume 30

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InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels (continued)

Figure 1: Tissue-specific tissue spindles ensure proper fit to the rotating core for even exposureto bathing media.

Figure 2: Sealable access port through the chamber wall creates a conduit to the hollow-milledtissue spindle enabling clean access to the bronchial lumen for seeding, in this case, withepithelial cells from the host.

Figure 3: The semi-circular “half pipe” chamber floor creates a shallow well for externalseeding, here with chondrocytes derived from bone marrow stem cells of the host. Mixing postson the rotating tissue spindle maintain cells in suspension for even deposition on the bronchialsurface.

Figure 4: Bioreactor developed forairway tissue engineering. (B)Bioreactor with the graft in situ. (C)Bioreactor after removal of thegraft. (D) The final graftimmediately before surgicalimplantation. (E) Schematic lateralview highlighting the rotation ofthe matrix around its longitudinalaxis. The design has separatecompartments for lumen and outersurface, and is regularly rotatedthrough a motor apply the sheerstress needed for growth distributenutrients and waste and ensureeven exposure to applied cells.

Bronchus recipient, Mrs. Castillo (and family), after successful bronchialtransplantation.

InBreath 3D Bioreactor Specifications

ROTATIONAL SPEED 0 - 5 RPM

DIAGNOSTICS Positional Monitoring

MATERIALS Polysulphone, Teflon, 318 Stainless Steel

VALIDATION CE

POWER 100 - 240 VAC, 50/60 Hz

Order # Product

RM1 73-4145 InBreath 3D Bioreactor for Hollow Organs, Bronchus, Trachea & Blood Vessels

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LB-2 Bioreactor for Mouse and Rat Lung RegenerationRegeneration and Respiratory Monitoring of Mouse and Rat Lungs from Decellularized Scaffolds

Physiological Maintenance and Monitoring:

The LB-2 provides an optimal, physiologically relevant environmentfor organ growth as well as the ultimate foundation for real-timemonitoring of respiratory mechanics. The complete range ofpulmonary monitoring parameters are fully integrated into thebioreactor to enable physiological monitoring during organ generationand end-stage validation for transplant.

• Airway Flow

• Airway Pressure

• Intrapleural Pressure

• Tidal Volume

• Minute Volume

• Pulmonary Resistance

• Pulmonary Compliance

• Pulmonary Artery Pressure

• Pulmonary Venous (Left Atrial) Pressure

The LB-2 represents the evolution of legendary Harvard Apparatus/Hugo Sachs Elektronik perfusion technology fromacute ex-vivo perfusion to Bioreactor based Organ Regeneration. Over 60years of whole organ physiological perfusion experience are at the coreof this first commercially available bioreactor for Lung Regeneration.

Like all Harvard Apparatus/Hugo Sachs Elektronik perfusion systems,the LB-2 provides unmatched physiological maintenance andmonitoring capabilities thanks to the patented Solid StatePhysiological Perfusion Circuit (S2P2C) technology. In the LB-2, thismeans rigidly milled pathways directly into the Perspex structure for

both vascular perfusion andairway ventilation. The result isprecisely repeatable non-turbulentperfusion and respirationcharacteristics. This, combinedwith the naturally excellentthermal properties of Perspex,creates a system that allowscontrol, maintenance, andmonitoring of vascular and respiratory mechanics duringregeneration in a way that is more physiologically relevant than anyconventional bioreactor.

System Accessories:

• Small Animal Ventilator • Pulsate Blood Pump

• Syringe Pump • Transducers

• Amplifiers • Data Acquisition and Analysis

References:1. Ott et al (2010) Regeneration and orthotopic transplantation of a bioartificial lung,Nature Medicine, Volume 16 No 8 2. Song et.al. (2011) Enhanced in vivo function of bioartifical lungs in rats, Ann ThoracSurg, Volume 92, No. 3

KEY FEATURESProven design. Demonstrated regeneration of rat lung

with physiological performance and subsequent

transplantation, as recently published by Ott et al, 2010,

and Song et al, 2004

Active control of both airway ventilation and vascular

perfusion during regeneration

On-line monitoring of respiratory mechanics and

vascular physiology

Self-contained bioreactor specially designed for unique

lung physiology

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Decellurized Lung

Note: Harvard Apparatus would like to thank Dr. Daniel Weiss for IRCI imaging assistance.

• Vascular Resistance

• Filtration Coefficient

• pH, pO2, pCO2

• Temperature

• Lung Weight


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Figure 1: Perfusion decellularization of whole rat lungs. (a) Photographs of a cadaveric rat lung,mounted on a decellularization apparatus allowing antegrade pulmonary arterial perfusion. pa,pulmonary artery; pv, pulmonary vein; tr, trachea; RUL, right upper lobe; RML, right middle lobe;LL, left lobe. Freshly isolated lung (left), after 60 min of SDS perfusion (middle), and after 120 minof SDS perfusion (right). The lung becomes more translucent as cellular material is washed outfirst from apical segments, then from the middle segments and finally from the basal segments.

Figure 3: Orthotopictransplantation and in vivofunction. (a) Photograph ofleft rat chest after anteriorthoracotomy, leftpneumonectomy, andorthotopic transplantationof a regenerated left lungconstruct. Recipient leftpulmonary artery (A), leftmain bronchus (B) and leftpulmonary vein (V) areconnected to regeneratedleft lung pulmonary artery(a), bronchus (b) andpulmonary vein (v). Whitearrowheads, the recipient’sright lung (infracardiac andright lower lobe); blackarrowheads, theregenerated left lungconstruct. (b) Radiograph ofrat chest after leftpneumonectomy andorthotopic transplanationof a regenerated left lungconstuct. Whitearrowheads, recipient’sright lung; black arrowheads, regenerated left lung construct. (c) Results of blood gas analysesshowing decrease in arterial oxygen tension (PaO2) after left pneumonectomy and partial recoveryafter orthotopic transplantation of a regenerated left lung construct. Baseline, single lung, 10 minand 30 min measurements were obtained with the rat intubated with the extubated and breathingroom air without support of a ventilator. Upper P values compare single lung ventilation to 5 min,and 30 min time points after transplantation at 5 min, 30 min and 6 h time points after operation.Error bars, s.d.

Phase 1: Decellularization Phase 2: Regeneration

Phase 3: Validation

Figure 2: In vitro functional testing of regenerated lung constructs. (a) Photographs ofregenerated lung contructs attached to the isolated lung apparatus) pa, pulmonary arterialcannula; pv, pulmonary venous cannula; tr, trachea) in expiration (left) and inspiration (right;RUL, right upper lobe; RML, right middle lobe). (b) Bar graphs showing results of blood gasanalyses of pulmonary venous effluent of native lung, HUVEC- and A549-seeded lung groupsshowed gas exchange function; pO2/FiO2 ratio and pCO2 did not differ between native and H-FLC-seeded lungs. (c) Line chart showing the dynamic pressure/volume relationship during fiverespiratory cycles of native, decellularized and regenerated lungs. Corresponding compliancevalues (ml/cm H2O/s are shown in boxes. (d) Bar graph comparing vital capacity of native(black), H-A549-seeded lung (gray) and H-FLC-seeded, regenerated lung (red). Error bars, s.d.

LB-2 Bioreactor for Mouse and Rat Lung Regeneration (continued)

The LB-2 is a Complete Lung Bioreactor for All Phases of Lung Regeneration

Order # Product

RM1 73-4213 ILB-2 Basic Unit for Lung Bioreactor, Size 2

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InspirationExpiration

Phase 4: Transplantation

References:1. Ott et al (2010) Regeneration and orthotopic transplantation of a bioartificial lung,Nature Medicine, Volume 16 No 8 2. Song et.al. (2011) Enhanced in vivo function of bioartifical lungs in rats, Ann ThoracSurg, Volume 92, No. 3


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KLB-2 Kidney and Liver Bioreactor

The system controls perfusion rate of thecells onto the scaffold, while monitoring andcontrolling pH, CO2 and O2 with sterilizableprobes. Proper mositure and gaseousenvironment for the organ are maintained.Infrared contrast imaging technology (IRCI)enables researchers to verify the intergrity ofthe internal perfusion path. A gentle headperfusion pump minimizes the crushing of cells often caused by a normal peristalic pump.

The organ to be perfused is placed in thechamber and perfused under constant-flow conditions. Thecentrifugal pump employed to reduce cell lysis is controlled by anelectronic controlller so that the apparatus can be operated underconstant-flow conditions. The system can also operate underconstant-pressure conditions. Flow control requires that the flow ismeasured by means of a flow probe and a flowmeter. Systempressure is continuously measured to monitor the experiment. Thisrequires a pressure transducer and a bridge amplifier. A suitableoxygenator can be employed for oxygenating and warming theperfusate. It can be a type used in human medicine, e.g. TerumoCapiox SX10®. Components required.

The following items are required for operating the apparatus:

• Thermostated moist chamber type 869/2

• Circulating thermostat of approximately 3 liter capacity,

distilled water and an anti-algal agent, e.g. thermoklar

• Pump with electrical control. For blood we recommend a

pump drive BVP-ZX with centrifugal pump head

• Servo controller for perfusion (SCP) type 704

• Flowmeter (TTFM) with flow probe

• Recommended: pressure transducer and bridge

amplifier (TAM-D)

• Oxygenator with heat exchanger, e.g. Terumo Capiox SX10®

Additional monitoring equipment can be added to the system asrequired to create a custom, application specific, turn key system foryour unique research needs.

Figure 1: A decellurized kidney in 3D bioreactor with user-defined perfusion lines set-up. Figure 2: The entire 3D bioreactor. From right to left organ chamber, peristaltic pump with gentle head,pH, O2, CO2 sensor control, data acquisition.

Figure 3: A decellurizedkidney. The perfusion was notstarted. It is visualizingthrough the intactdecellurized organ. The majorfeatures are shown very wellthrough 1 1/2 cm of tissuewith IRCI imaging.

Figure 4: The perfusion wasstarted and the red colorrepresents the flow throughthe perfusion vasculature.

Order # Product

RM1 73-9913 KLB-2 Kidney and Liver Bioreactor


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Linear Stress and Relaxation Bioreactor

The Linear Bioreactor assists in the engineering of tissue in aprogrammable modular platform which produces mechanical stressand relaxation. Modular platform performs reproducible mechanicalstress and relaxation. Customer defined parameters for:

- Frequency

- Distance

- Measurement Sensors

- Flow

KEY FEATURESPrecise control of biomechanical parameters

Accommodates virtually any linear tissue sample

Simple, robust tissue clamps for stable mounting

Materials and mechanicals withstand environment of

tissue culture incubator

Driven by Labview control interface

Figure 1: In vitro capillary formation of EC co-cultured with pericyte progenitors. A. HUVEC and10T 1⁄2 co-culture. HUVEC were labeled green with Vybrant® CFDA SE Cell Tracer and 10T 1⁄2mouse embryonic pericyte progenitors were labeled red with red fluorescent CM-Dil. The labeledcells were mixed with 1:3 metrigetcollagen suspension and planted in culture dished in DMEM +10% FBS. Capillaries containing EC an pericytes were observed in cultures of HUVEC alone andin co-cultures. IN HUVEC alone, capillaries were dissolved after about a week whereas in co-cultures, capillaries remained stable for at least 3 weeks (shown in A). B. HUVEC, mousemyoblasts and 10T 1⁄2 co-culture. HUVEC were labeled with red fluorescent CM-Dil and mousemyoblasts were labeled green with Vybrant® CFDA SE Cell Tracer. 10T 1⁄2 were unlabeled. Co-culture of the 3 cell types show capillaries embedded within the muscle fibers.

Figure 2: (a) Matureskeletal muscle fibersderived from myoblastsgrown on BAM scaffoldand explanted from thesubcutaneous space of anude mouse 8 weeksafter implantation. (b)Tichrome stain indicatingmuscle tissue (red) andblue collagen from thescaffold. (c) Desminimmunostain indicatingthe presence of skeletalmuscle fibers. (d) Redfluorescence indicatingthe integration of HUVECsin the vasculature withinthe scaffold 10 weeksafter being labeled withCM-Dil.

Order # Product

RM1 88-0242 3D Stress and Relaxation 3D Bioreactor

Front view

Rear view with connectors

Camera mounted Top view

APPLICATIONSBone

Skeletal Muscle

Tendon

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Cartilage


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High Precision 3-Axis Imaging PlatformFor Visualizing Organ Generation in a Bioreactor from a remote location

3-Axis Imaging Platform Specifications

in mm

FRAME (OVERALL DIMENSIONS)

HEIGHT 16.688 423.88

LENGTH 15.483 393.26

WIDTH 16.285 413.65

LINEAR RAIL STROKES

X-AXIS 10.000 254.00

Y-AXIS 6.000 152.00

Z-AXIS 3.000 76.20

LINEAR RAILS (INCLUDING MOTOR)

X-AXIS

HEIGHT 1.661 42.18

LENGTH 16.285 413.65

WIDTH 2.000 50.80

Y-AXIS

HEIGHT 1.661 42.18

LENGTH 16.285 413.65

WIDTH 2.000 50.80

Z-AXIS

HEIGHT 1.661 42.18

LENGTH 9.285 235.85

WIDTH 2.000 50.80

REPRODUCIBILITY 0.002 0.05

Z-AXIS LINEAR RAIL CAMERAMOUNTING HOLES (SEEDIAGRAM RIGHT)

MOTOR TYPENEMA 17 Stepper Motor0.9° Step Angle

MINIMUM SPEED .002 in/s .04 mm/s

MAXIMUM SPEED .156 in/s 3.97 mm/s

VOLTAGE RANGE 12V Nominal; 9V - 30V

NUMBER OF MICROSTEPS PERONE REV OF LEAD SCREW

6400

INPUT POWER 12Vdc, 1.5A

NET WEIGHT 8 lbs.

KEY FEATURESA small footprint

Built with USP class VI materials making it well suited

for standard incubator environments

The modular design and accompanying motion-control

system allow users to incorporate any one of several

imaging modalities

This 3-axis positioner provides researchers with the first means fornon-invasive, real-time imaging of cell cultures, regenerating organsand biomaterials, reducing both laboratory costs and risk ofcontamination. The controller makes it easy to investigate macro ormicro sections of the organ.

Order # Product

RM1 88-0243 3-Axis Imaging Platform


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Harvard Apparatus Accelerator 1000 Multi-well Imaging Bioreactor and IncubatorA Powerful NEW Tool for Enhanced Regenerative Medicine Research

BIOREACTORS

Accelerate your research with higher quality andmore accurate data, fast!

After speaking with hundreds of our customers, we discovered thatto study all the permutations necessary to successfully regeneratean organ, a new tool must be developed to change the researchmodel used to study these variables. The best way to optimizedecellularization or recellularization is an imaging bioreactor whichhas the ability to study experimental variables in a combinatorialmanner:

- Which cell line to use?

- Which scaffold, natural or synthetic, has the greatest

adherence to cell types?

- Which surfactant is best for decellularization concentrations

soak & flush times?

- Ideal perfusion composition: pH, number of cells needed,

temperature, buffer molarity, structure flexibility, resistance

- Add active stimulation (mechanical or electrical) to the

decellularization process

- Use resistance to characterize decellularization process to

measure cell stripping

- Remove or add growth hormones or apoptosis signalling

chemical or cellular waste: use dialysis to remove molecules,

use SPF traps to trace enrich for analysis

- Measure immunoresponse of scaffold to cells

- Use Infrared Imaging (IRCI) to monitor decellularization

process (tissue structure and anatomy)

- Use visible microscopy to determine confluence (3-Axis

Imaging Platform)

- What temperature to use at what time?

- What stage of decellularization is best for what kind of cells?

The new Accelerator 1000 muti-well bioreactor/incubator is animaging perfusion chamber with four active 35 mm perfusionchambers and interchangeable templates that can have up to 96wells of immersion imaging perfusion. The system is ideal forstudying the variables of decellularization or recellularization induplicate in a short time period.

The flexibility of the system allows a high throughput screeningphase (up to 96 wells) to be followed by lower throughput stages(down to 4 wells of controlled perfusion) of increasing experimentalcontrol and complexity.

The world of combinatorial analysis has many advantages:

• Smaller sample size allows for imaging up to 96 different

chambers in practically the same time frame

• Smaller samples reduce the amount of expensive experimental

variable: cell lines, growth factors

• More detailed analysis in the same time...ability to get more

data which provides the optimal information to make better

decisions faster

• Reduced cost of experiments for expensive or hard to get

components


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Harvard Apparatus Accelerator 1000 Multi-well Imaging Bioreactor and Incubator (continued)

Accelerate your research with higher quality and more accurate data fast

Ideal for establishing and ascertaining the properconditions for cell, tissue and organ regeneration

• Combinatorial Bioreactor 1, 2, 4, 6, 12, 24, 48 and 96 wells of

imaging and environmental control and solution exchange

• Up to four channels of active perfusion

• Experiments:

- Enhance cell and scaffold selection with duplicate

experiments of cell and scaffold adherance studies

- Enhance decellularization and recellularization formulas run

in duplicate to find optimal conditions. Vary pH, Time,

Temperature, detergents, gas % environment O2/CO

2,

mechanical motion, electrical stimuli, gravity, scaffold

coatings and scaffold types.

- Generate multiple tissues and organs in animal model with

optimized conditions, cell and scaffold choices, to accelerate

experiment to get to first-in-man.

Software can capture images at certain times for post analysis

Imaging Reactor Plates

Insertion Templates

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Harvard Apparatus Accelerator 1000 on Microscope Stage

Order # Product

Harvard Apparatus Accelerator 1000

RM1 99-0269 Basic MIS 2 Well SI

RM1 99-0271 Basic MIS Multiwell SI

RM1 99-0271ASI Basic MIS for ASI Stages

RM1 99-0275 Petri Dish 2-Well Plate Adapter

RM1 99-0277-6 6-Well Plate Adapter




RM1 99-0277-96 96-Well Plate Adapter for both Basic and Cyro Multi-Well

RM1 99-0266 Petri Dish and Chamber Slide Adapter

RM1 99-0277-4 Four Petri Dish Plate Adapter

Optional Accessories

RM1 99-0278 Third Gas Flowmeter

RM1 99-0279 Adapter, round for 25 mm cover slips 1.5 thick, suitable for both ID and Oilimmersion objectives observationwindow: 18 mm

HT Imaging Bioreactor Specifications

Multi-WellAdvantage 24 Well

Multi-WellAdvantage 96-Well

OneExperiment at a Time

NUMBER OF TESTS DONE 24 96 8

NUMBER OF VARIABLES TESTED 8 24 8

SAMPLES RUN IN TRIPLICATE 3 3 1

TOTAL TIME 3 Days 3 Days 3 Days

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3 6 9 12 19 25

Enzyme 1

Enzyme 2

Sufractant A

Sufractant B

Example Experimental format

1-4 Channels of Active Perfusion in 35 mm

Harvard Apparatus Accelerator 1000 Multi-well Imaging Bioreactor and Incubator (continued)

Experimental Models: Multiple conditions in a single plate to accelerate data collection forhigh throughput and screening assays

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RC-37F and RC-37WCell Culture Dish Perfusion Chamber Insert

Top View

The introduction of thin glass bottom culture dishes has expandedthe use of these popular tools dramatically. Applications such as livecell imaging, confocal microscopy and time-lapsed photography arenow possible. The ability to exchange the extracellular environmentby perfusion 35 mm dishes has been difficult at best, until now.

The RC-37 Perfusion Chamber Inserts for cell culture dishes makesperfusion within these dishes a simple matter. Fluid is delivered to aperfusion inlet port and removed from the chamber by means of aheight adjustable aspirator. A thin coating of silicone grease forms aseal between the insert and dish.

Inserts are available to fit the Willco Wells, Corning, MatTek, Nuncand Falcon 35 mm dishes. The chamber is compatible with the DH-35i and DH40i Culture Dish Incubators and the QE-1 QuickExchange Platform.

Each chamber is supplied with 1 foot of C-Flex tubing and 10 feet of PE-160 tubing.

KEY FEATURESDesigned for glass bottom 35 mm culture dishes

Permits imaging, recording, and perfusion in

the culture dish

Open and closed bath designs

Incorporates diamond fluidics

Ideal for rapid screening assays

Aspirator

Agar Bridge Well

Perfusion Inlet Port


RM1 64-0348 RC-37F Chamber Insert for Corning, MatTek, Nunc,and Falcon 35 mm Dishes

34.5 mm

15.2 mm

Volume: 220 µl

RC-37F

The RC-37 family of perfusion chamber inserts makes perfusion ofcell culture dishes a simple matter. The closed bath promotes aneven solution exchange and eliminates changes in focus due toalterations in bath height. In addition, studies involving physiologicalbicarbonate buffers can be performed with no gas loss at thechamber interface.

Fluid is delivered to a 18 gauge perfusion inlet port and removedfrom the chamber by means of an outlet port of the same gauge.The top coverslip is held in place with a retainer and the dishbecomes the chamber bottom. A thin coating of silicone greaseforms the seal between chamber and dish. The distance betweentop and bottom coverslips is 1 mm. The RC-37WC uses 15 mm topcoverslips and the RC-37FC works with 18 mm coverslips.

The RC-37WC fits in the Willco Wells dishes while the RC-37FC fitsNunc, Falcon, MatTek or Corning 35 mm dishes. Warner’s DH-35Culture Dish Heater may be used to heat the Corning, MatTek andWillco Wells 35 mm culture dishes. Warner’s DH-35i and DH-40iCulture Dish Incubators and the QE-1 Quick Change Heated Baseare compatible with all 35 mm culture dishes.

The RC-37WC is supplied with one box of #1 thickness coverslips (CS15R, 15 mm round, 100/pkg) and the RC-37FC is supplied withone box of #1 thickness coverslips (CS18R, 18 mm round, 100/pkg).Both chambers are supplied with 10 feet of PE-160 polyethylenetubing and tools to remove the top coverslip retainer.

Top View

34.0 mm

RC-37FC

15.2 mm

Volume: 185 µl

Volumes are for 1 mm solution height

Perfusion Outlet Port

Perfusion Inlet Port

Top CoverslipRetainer

KEY FEATURESDesigned for glass bottom 35 mm dishes

Permits imaging and perfusion in the cell culture dish

Incorporates diamond fluidics

Ideal for rapid screening assaysOrder # Model Product

RM1 64-0364 RC-37FC Chamber Insert Closed for Corning, MatTek, Nuncor Falcon 35 mm Dishes

RM1 64-0363 RC-37WC Chamber Insert Closed for Willco 35 mm Dishes

Accessories and Replacement Parts

RM1 64-0703 CS-15R Coverslips 15 mm diameter #1 Thickness – Box of 100

RM1 64-0384 CS-18R Coverslips 18 mm diameter #1 Thickness – Box of 100

RM1 64-0758 D3522P Glass Bottom Cell Culture Dishes – 20/Pouch

RM1 64-0762 D3522B Glass Bottom Cell Culture Dishes – Pkg of 120

RM1 64-0378 111-KIT Silicone Grease Kit with Brushes and Pallets

RM1 64-0110 DH-35 35 mm Culture Dish Heater

RM1 64-0349 DH-35i Culture Dish Incubation System

RM1 64-0388 DH-40i Culture Dish Incubation System

RM1 64-0375 RC-37FC Quick Exchange Heated Base Perfusion and Adapter Ring Kit


BIOREACTORS

RC-37FC and RC-37WC Closed bath chamber for continous looped organ perfusion

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Electric CO2 Microscope Stage Incubatorfor Multiwell Studies of 3D Organs and Production Variable

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The Electric CO2 Microscope Stage Incubator is the latest release inthe family of Stage Top Incubators. It represents a one-buttonsolution for long term experiments. The same model fits all the XYstages on the market and it is suitable at the same time for highmagnification microscopy and multipoint experiments. A wide choiceof interchangeable inserts adds flexibility to the equipment andaccepts any cell culture support (Petri-dishes, glass slides, multiwellplates, etc.).

Temperature Regulation

In the Electric Microscope Stage Incubator, specimen temperature isregulated by the combined action of two controllers acting on thepower dissipated by the electric resistances embedded both in thebase and in the lid of the incubating chamber. Two temperaturefeedback mechanisms are available:

• Chamber temperature feedback

• Specimen temperature feedback

In the Chamber temperature feedback mode a thermocouple readsthe temperature of the incubating chamber. A careful calibrationperformed in our laboratories guarantees that specimen temperatureis maintained at the desired value. The advantage of this solution isthat the thermocouple is not visible since it is embedded into thechamber.

In the Specimen temperature feedback mode a thermocouple wirereads the temperature of a reference well placed in the incubatingchamber very close to the specimen. The advantage of thisconfiguration is the accuracy of the temperature control.

CO2

Regulation

CO2 is mixed with air in the supplied control unit and this mixture iscontinuously fed into the incubating chamber to control medium pH.An optional third gas line can also be fed into the controller.

Humidity Regulation

A humidifying and a pre-heating module (optional) preventevaporation of medium and avoids water condensation within thechamber.

Software (optional)

Read Temperature Software is available to read the incubatortemperature and to store the data in computer memory.

KEY FEATURESLow cost solution for long term experiments

One model fits all XY stages

Suitable for high magnification microscopy

Temperature control from Ambient + 3°C to 50°C

Wide selection of cell culture support adapters

Electric CO2

Microscope Stage Incubator Specifications

TEMPERATURE RANGE Ambient + 3°C to 50°C

ACCURACY ±0.3°C (Chamber temperature feedback)

HEATING TECHNOLOGY Electric

TYPE OF CONTROLLER Hardware

TEMPERATURE FEEDBACK Chamber temperature feedbackSpecimen temperature feedback (optional)

HUMIDIFICATION MODULE Not heated (optional heated version)

CO2 RANGE 0 to 100%


(L x W x H) 159 x 110 x 18 mm (6.26 x 4.33 x .71 in)

WEIGHT 405 g

APPLICATIONSCell/Scaffold adhesion studies

Optimal fluid

4-channel active perfusion 3D Bioreactor


BIOREACTORS

Electric CO2 Microscope Stage Incubator Accessories

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H301-EC

Universal electrically heated chamber, fits any mechanical andmotorized XY stage. Requires at least one plate adapter. Thecondenser working distance is 22 mm without chamber riser and28 mm with chamber riser.

H301-EC-LID

A 1 mm glass lid with heated metal frame.Requires at least one LID adapter and oneBASE adapter.

H301-EC-HG

A 2 mm transparent heated lid.Requires at least one BASE

adapter.

H301-EC-6MWLID

6-well plate Lid adapter.

H301-EC-12MWLID


H301-EC-24MWLID


H301-EC-96MWLID


H301-EC-48MWLID


H301-EC-35LID

Four 35 mm Petri dish LID adapter.

H301-EC-35BASE

Four 35 mm Petri dish BASE adapter.


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Digital Gas Mixer DGTCO2BX (-D-RS232)

set point is 5%, accuracy is ±0.25%. DGTCO2BX can be used as astand-alone device as well as in combination with any kind ofmicroscope incubator. The air flow is regulated by a floating ball airflow meter in the range 0.2 to 0.8 Nl/min.

Sensing Technology

A CO2 infrared sensor continuously measures CO2 concentration inthe mixed gas stream and a PID closed loop controller givesfeedback to a fine valve regulating CO2 flow. The measured value ofCO2 concentration is displayed in real time in green. An alarm can beset on CO2 concentration so that if CO2 concentration goes belowset value, the display color turns red and a buzzer turns on.

Digital Gas Mixer

The DGTCO2BX is a Digital Control Unit that generates a CO2-Airmixture with an adjustable CO2 concentration in the range 0-20%,with an accuracy of ± 5% of CO2 concentration, for example, if CO2

KEY FEATURESWorks with any brand microscope incubator

CO2

concentrations in the 0-20% range

Stand alone or Software driven

Accessories:

- gas filter

- pressure gauge for CO2

with regulator

- two white rigid tubes of 6 mm O.D. 3 m long

- one blue rigid tube of 6 mm O.D. 2 m long

- RS-232 serial cable (-D-RS232 version only)

- CD with control software (-D- RS232 version only)

Digital Gas Mixer Specifications

SENSING TECHNOLOGY Non dispersive infrared (NDIR) dual wave length decator

CO2 SET POINT VALUE RANGE 0-20%

ACCURACY ±5% of CO2 concentration, i.e. ±.25% if CO2 is 5%

REPEATABILITY OF CO2CONCENTRATION

Below 0.1%

AIR FLOW RATE From 0.2–1.8 NI/min

FILTERING DEVICE Reusable PTFE membrane with 0.2 mm pores

DIGITAL INTERFACE (-D-RS232) RS-232 to PC

COMMUNICATION SOFTWARE(-D-RS232)

Stand alone version and Plug-in Version for all Okolab software

COMPATIBILITY Any microscope incubator

VOLTAGE/FREQUENCY 110-230 VAC/50-60 Hz


(L x W x H) 206 x 178 x 310 mm (8.1 x 7 x 12.2 in)

WEIGHT 3.5 kg


RM1 99-0486 DGT-CO2BX Digital Gas Mixer (stand alone)

RM1 99-0487 DGT-CO2BX (-D-RS232)

Digital Gas Mixer (stand alone with serial cableand software)

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Two Gas Mixer

The 2GF_Mixer is a two gas mixer, it is generally used to generateCO2- Air mixtures with an adjustable CO2 concentration in the range0-15%. 2GF_Mixer can be used as a stand-alone device as well as incombination with any kind of microscope incubator.

Air and CO2 flows are regulated by two floating ball flow meters inthe range 0.2 – 1.7 Nl/min and 0.013 – 0.13 Nl/min, respectively. Asan example, Table 1 reports a set of flow rates to obtain aconcentration of CO2 equal to 5%.

A more Comprehensive table is reported in the manual.

KEY FEATURESWorks with any brand microscope incubator

CO2

concentrations in the 0-15% range

Reusable PTFE membrane filter

Accessories:

- gas filter

- pressure gauge for CO2

with regulator

- two white rigid tubes of 6 mm O.D. 3 m long

- one blue rigid tube of 6 mm O.D. 2 m long


RM1 99-0484 2GF_Mixer Two Gas Mixer

Two Gas Mixer (2GF_Mixer) Specifications

SECOND GAS SET POINT RANGE

0-15%

ACCURACY OF SECOND GAS CONCENTRATION

±0.1%

FILTERING DEVICE Reusable PTFE membrane with 0.2 mm pores

COMPATIBILITY Any microscope incubator


(L x W x H) 220 x 128 x 178 mm (8.67 x 5 x 7 in)

WEIGHT 1.9 kg

AIR / CO2 FLOW RATES TO OBTAIN A 5% CO2 MIXTURE

TARGET GAS % GAS FLOW RATE VALUES TO SET (NI/MIN)

Air CO2 Air CO2 (%) Total

95.0 5.0 0.8 0.04 0.84

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Digital Gas Mixer DGTCO2BX (-D-RS232) (Continued)

Performance Assessment

The charts below report CO2 concentration as a function of time in afixed set point value experiment and in a step change experiment.

CO2 concentration during 2-days experiments CO2 concentration in step up and step down experiments

HPC-3, Hydrostatic Perfusion Chamber for Organand Tissue Decellularization

Detergent or enzyme based decellularization of tissues and organscan be a cumbersome process, with multiple bottles of differentsolutions all leading to the tissue through a convoluted tubingnetwork. The HPC-3 is a practical solution, adding useful featuresand enabling standardization of decellularization protocols for anytissue. Three independent non-heated glass vessels are mounted ona robust vertical stand to provide the required hydrostatic perfusionpressure and all required tubing, in appropriate lengths, is included.Glass vessels and the included Teflon Lids are fully autoclavable.

Order # Product

RM1 73-001020 3-Fold Hydrostatic Perfusion Chamber with Stand


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C-Pace Cell Culture EP StimulatorPrevent de-differentiation in isolated cell cultures through electrical conditioning

The C-Pace EP is a multi-channel stimulator designed for the chronicstimulation of bulk quantities of cells in culture incubators. This paceremits bipolar stimuli, greatly reducing electrolysis at the electrodes.It has an output voltage of +/- 40 V, a frequency from once every 100seconds to 99 pulses/sec, and duration 0.4 to 24 msec. The EP hasflexible pacing protocols that have been designed on request bycustomers who want to study the effects of arrhythmia or exerciseprotocols during culture. Gate and Aux outputs allow for increasedfunctionality when used as an acute pacer.

KEY FEATURESArrhythmia Protocols

- Insert an offbeat pulse at a fixed interval

Exercise Protocols

- Run multiple pulse trains with individually

programmable frequency and duration

Irregular Pacing

- Random variation of specified frequency within

definable percentage window and guaranteed

average effective rate

External Triggering

- Change to next pulse train with TTL “advance”

input and/or trigger individual pulses with TTL

pulse input

APPLICATIONSCardiac Tissue

Skeletal Muscle

Stem Cells

Cell-lines

Cardiogenic Fibroblasts

Neuronal Fibroblasts

Osteoblasts

There are several benefits to chronic electrical stimulation. Therationale, however, is always the same: treat the cells in a mannerconsistent with their native environment. Like using propermaintenance media and incubation, chronic culture pacing will helpinduce and/or maintain phenotype over time. The C-Pace EP can beconfigured with up to 8 channels and electrode assemblies forcommon 4-, 6-, and 8-well culture dishes are available. Our chronicpacers have been used to culture a variety of cells that areelectrically coupled in vivo, including smooth and striated muscle.They have also been used to facilitate the differentiation of stemcells. Looking for a better way to culture cells? Try the C-Pace EP.

4 Well 6 Well

8 Well 35 mm


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5 Years of the C-Pace: A short review

It has been more than 5 years since Ionoptix brought a device to thescientific research community that allowed chronic electricalstimulation of cells in culture. Since the introduction of the C-Pace,researchers have found a wide range of applications for it, for manydifferent cell types. Here we provide a brief review of the history ofthe chronic electric stimulation of cells in culture, the applications itis being used for and citations for the relevant papers.

History

We started working on a device for chronic electrical stimulation ofcells in culture after requests from several cardiac research groups.A paper by the group of Ralph Kelly and Thomas Smith (Berger, et al.1994) showed the benefits of pacing adult cardiac myocytes inculture. Mechanical activity of myocytes in culture allowed them toretain their phenotype while retaining their contractile characteristics.This work was elaborated on in later years by the group of GeorgeCooper and Paul McDermott. They showed that chronic pacing notonly allowed the retention of phenotype(Kato, et al. 1995) but alsoenhanced protein synthesis (Ivester, Kent, et al. 1993)(Ivester,Tuxworth, et al. 1995). Based on their work and ideas we decided todevelop a commercially available multi-channel pacer.

Cardiac muscle; adult, neonatal, embryonic and cell lines

Early users of the C-Pace confirmed the usefulness of the C-Paceunit for myocyte culture. It can be used to establish a stable primaryculture that, for example, allows expression of proteins usingadenovirus transfection while maintaining the ability to studyexcitation-contraction coupling (Mills, et al. 2006)(Ahlers 2005). Thechronic pacing of cells does alter signaling pathways favorably butcan also predispose a fraction of the cells to apoptosis (Kuramochi,Guo, et al. 2006)(Kuramochi, Lim, et al. 2004). It is possible that theinflexible surface of culture dishes causes a problem. A futuredevelopment could be the use of flexible substrates to better mimicphysiological conditions.

Apart from chronic stimulation, the system has also been used forshort-term bulk stimulation of cultured cells to achieve a variety ofeffects. Examples are to achieve the translocation of calmodulinkinase (W. Thiel, et al. 2008) or transcription factors from thecytoplasm to the nucleus (Schroder, Byse and Satin 2009). Anotherreport indicates that chronic pacing during virus transfectionincreases transfection efficiency (Rana, et al. 2008). Increasing themechanical activity with pacing induces elevated myocytemetabolism. Field stimulation of large quantities of cells has beenshown to be a useful tool in the study of myocyte metabolism(Luiken, et al. 2001).

Interesting studies have been performed using the C-Pace system tomimic tachycardia. Brundel et al used it to look at stress responsesin HL1 cell lines upon induced tachycardia (Brundel, Henning, et al.2006)(Brundel, Shiroshita-Takeshita, et al. 2006). The group of S.Nattel has published a very elegant study showing electricalremodeling in cultured canine myocytes with high frequency pacingover a 24 hour period (Qi, et al. 2008). This is one of several studiesto show that pacing exerts its initial effect by activating L-typecalcium channels and thus elevating intracellular calcium levels. L-type calcium channels are obviously important for calciumhomeostasis in adult cardiac cells, but also suggest that pacing canbe used to great effect on non-excitatory cells.

Works Cited

Ahlers, B. A. "Effects of sarcoplasmic reticulum Ca2+-ATPase overexpression inpostinfarction rat myocytes." Journal of Applied Physiology 98, no. 6 (Feb2005): 2169-2176.

Berger, H J, et al. "Continual electric field stimulation preserves contractilefunction of adult ventricular myocytes in primary culture." The American journalof physiology 266, no. 1 Pt 2 (Jan 1994): H341-9.

Brundel, Bianca J J M, et al. "Induction of heat shock response protects theheart against atrial fibrillation." Circulation Research 99, no. 12 (Dec 2006):1394-402.

Brundel, Bianca J J M, Robert H Henning, Lei Ke, Isabelle C van Gelder, HarryJ G M Crijns, and Harm H Kampinga. "Heat shock protein upregulation protectsagainst pacing-induced myolysis in HL-1 atrial myocytes and in human atrialfibrillation." Journal of Molecular and Cellular Cardiology 41, no. 3 (Sep 2006):555-62.

C-Pace Cell Culture EP Stimulator (continued)

Cardiogenic fibroblasts, stem cells and bonemarrow cells

The work of Genovese and colleagues show the potential ofelectrostimulation for stem cell differentiation and provides clueswhy electrostimulation has such a strong effect on musclephenotype. In an initial study the group showed thatelectrostimulation can induce cardiac pre-commitment of stem cellsor fibroblasts (J. Genovese, et al. 2008). It possibly does so by theupregulation of follistatin which is considered to be a key modulatorin muscle development, differentiation and regeneration’ (J.Genovese, et al. 2009).

Skeletal muscle

The C-Pace has been used successfully on skeletal preparations. Acouple of papers have been published employing C2C12 cell lines,where pacing can be used to induce de-novo sarcomere formation inincompletely differentiated myotubes (Fujita, Nedachi and Kanzaki2007). Alternatively, in fully differentiated myotubes, stimulation canbe used to achieve a hypertrophic response, rather similar to thesignaling programs seen in intact muscle upon exercise (Nedachi,Fujita and Kanzaki, American Journal of Physiology- EndocrinologyAnd Metabolism 2008)(Nedachi, Hatakeyama, et al. 2009).

Other uses; nerve cells and osteoblasts

The potential scope for chronic pacing is almost limitless as manycell types contain voltage sensitive channels. In neuronal fibroblastselectrical stimulation can be used to modulate the differentiation ofembryonic stem cells to a neurological phenotype (Yamada, et al.2007). The C-Pace system has been used in neuronal fibroblasts toinduce expression of the NMDA receptor by activating L-typecalcium channels (Okutsu, et al. 2008). Another application is in theculture of osteoblasts, where low level currents can help with theattachment to artificial surfaces and enhance proliferation ofosteoblasts (Ercan and Webster 2008).

Future

The list of uses for the C-Pace system will likely expand as it isapplied to many different types of experiments. We will strive tokeep improving the design of the C-Pace and the C-Dishes tosupport ongoing research and new uses. The device was developedin close cooperation with the scientific community and we will try tokeep the communication going so that we can improve the designaccording to your needs.

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C-Pace Cell Culture EP Stimulator (continued)

Ercan, Batur, and Thomas J Webster. "Greater osteoblast proliferation onanodized nanotubular titanium upon electrical stimulation." Internationaljournal of nanomedicine 3, no. 4 (Jan 2008): 477-85.

Fujita, Hideaki, Taku Nedachi, and Makoto Kanzaki. "Accelerated de novosarcomere assembly by electric pulse stimulation in C2C12 myotubes."Experimental cell research 313, no. 9 (May 2007): 1853-65.

Genovese, J, C Spadaccio, J Langer, and J Habe. "Electrostimulation inducescardiomyocyte predifferentiation of fibroblasts." Biochemical and BiophysicalResearch Communications, Jan 2008.

Genovese, Jorge A, Cristiano Spadaccio, Hernan Garcia Rivello, YoshiyaToyoda, and Amit N Patel. "Electrostimulated bone marrow humanmesenchymal stem cells produce follistatin." Cytotherapy 11, no. 4 (Jan 2009):448-56.

Ivester, C T, et al. "Electrically stimulated contraction accelerates proteinsynthesis rates in adult feline cardiocytes."The American journal of physiology265, no. 2 Pt 2 (Aug 1993): H666-74.

Ivester, C T, W J Tuxworth, G Cooper, and P J McDermott. "Contractionaccelerates myosin heavy chain synthesis rates in adult cardiocytes by anincrease in the rate of translational initiation." The Journal of biologicalchemistry, 270, no. 37 (Sep 1995): 21950-7.

Kato, S, C T Ivester, G Cooper, M R Zile, and P J McDermott. "Growth effectsof electrically stimulated contraction on adult feline cardiocytes in primaryculture." The American journal of physiology 268, no. 6 Pt 2 (Jun 1995): H2495-504.

Ke, Lei, et al. "Calpain mediates cardiac troponin degradation and contractiledysfunction in atrial fibrillation." Journal of Molecular and Cellular Cardiology45, no. 5 (Nov 2008): 685-93.

Krüger, Martina, Christine Sachse, Wolfram H Zimmermann, ThomasEschenhagen, Stefanie Klede, and Wolfgang A Linke. "Thyroid hormoneregulates developmental titin isoform transitions via the phosphatidylinositol-3-kinase/ AKT pathway." Circulation Research 102, no. 4 (Feb 2008): 439-47.

Kuramochi, Yukio, Chee Chew Lim, Xinxin Guo, Wilson S Colucci, Ronglih Liao,and Douglas B Sawyer. "Myocyte contractile activity modulates norepinephrinecytotoxicity and survival effects of neuregulin-1beta." American journal ofphysiology Cell physiology 286, no. 2 (Feb 2004): C222-9.

Kuramochi, Yukio, Xinxin Guo, Douglas B Sawyer, and Chee Chew Lim. "Rapidelectrical stimulation induces early activation of kinase signal transductionpathways and apoptosis in adult rat ventricular myocytes." Exp Physiol 91, no. 4 (Jul 2006): 773-80.

Luiken, J J, J Willems, G J van der Vusse, and J F Glatz. "Electrostimulationenhances FAT/CD36-mediated long-chain fatty acid uptake by isolated ratcardiac myocytes." Am J Physiol Endocrinol Metab 281, no. 4 (Oct 2001): E704-12.

Mills, Geoffrey D, Hajime Kubo, David M Harris, Remus M Berretta, ValentinoPiacentino, and Steven R Houser. "Phosphorylation of phospholamban atthreonine-17 reduces cardiac adrenergic contractile responsiveness in chronicpressure overload-induced hypertrophy." Am J Physiol Heart Circ Physiol 291,no. 1 (Jul 2006): H61-70.

Nedachi, T, H Fujita, and M Kanzaki. "Contractile C2C12 myotube model forstudying exercise-inducible responses in skeletal muscle." American Journal ofPhysiology- Endocrinology And Metabolism, Jan 2008.

Nedachi, T, H Hatakeyama, T Kono, M Sato, and M Kanzaki. "Characterizationof contraction-inducible CXC chemokines and their roles in C2C12 myocytes."Am J Physiol Endocrinol Metab, Jul 2009.

Okutsu, S, H Hatakeyama, M Kanazaki, and H Tsubokawa. "Electric PulseStimulation Induces NMDA Glutamate Receptor mRNA in NIH3T3 MouseFibroblasts." The Tohoku Journal of Experimental Medicine, Jan 2008.

Qi, X. Y, et al. "Cellular Signaling Underlying Atrial Tachycardia Remodeling ofL-type Calcium Current." Circulation Research 103, no. 8 (Oct 2008): 845-854.

Rana, O, E Saygili, C Meyer, C Gemein, and A Krüttgen …. "Regulation ofnerve growth factor in the heart: The role of the calcineurin–NFAT pathway."Journal of Molecular and Cellular Cardiology, Jan 2008.

Saeko. "Microsoft Word - îéémò_ï∂0627âúí√_íÒèo_." Jun 2008: 1-21.

Schroder, Elizabeth, Miranda Byse, and Jonathan Satin. "L-type calciumchannel C terminus autoregulates transcription." Circulation Research 104, no.12 (Jun 2009): 1373-81.

Thiel, W. H, et al. "Proarrhythmic Defects in Timothy Syndrome RequireCalmodulin Kinase II." Circulation 118, no. 22 (Nov 2008): 2225-2234.

Yamada, Masahisa, et al. "Electrical stimulation modulates fate determinationof differentiating embryonic stem cells."Stem Cells 25, no. 3 (Mar 2007): 562-70.

Order # Product

RM1 72-9460 C-PaceEP Cell Culture EP Stimulator + EHV100

RM1 72-9461 High Voltage Output Bank for C-PaceEP

RM1 72-9462 4-Well C-Dish Culture Dish Electrode System. Fits NunclonPolystrene Rectangular Well Dishes or Multidishes.

RM1 72-9463 6-Well C-Dish Culture Dish Electrode System. Fits Nunclonand Corning 35 x 10 mm Dishes.

RM1 72-9464 6-Well C-Dish Culture Dish Electrode System. Fits FalconBrand 35 x 10 mm Dishes.

RM1 72-9465 6-Well C-Dish Culture Dish Electrode System. Fits Falcon andCorning 6-Well Plates.

RM1 72-9468 6-Well C-Dish Culture Dish Electrode System. Fits FlexcellBioflex and Uniflex Plates.

RM1 72-9464 6-Well C-Dish Culture Dish Electrode System. Fits Nunclon 8-Well Rectangular Dishes or Multidishes.


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Stimulator C

The Stimulator C is a compact, highly stable square wave pulsestimulator that features precise digital timing control. A large LCDdisplay (2 lines at 40 characters) and minimum of controls makesetup and operation easy. All relevant stimulation parameters areshown on the display which provides a rapid view of the currentsettings. Several stimulation modes are possible (repetitive, single,triggered, tetanic...).

The last settings used are always stored in memory and recalledwhen the unit is turned on. Two stimulation pattern can bepredefined and stored in the non volatile memory. These pattern caneasily be recalled by selecting it in the menu.

The basic rhythm can be displayed either as frequency in Hertz or as period in msec respectively sec. The adjustment of the timing iscontrolled by a microcontroller to avoid wrong settings.

The powerful (20 VA) and fast (rise and fall time < 1µs) constantvoltage (up to 50 Volt) output allows stimulation of all types oftissues from direct contact nerve to tissue bath field stimulation. Theoutput is floating, not connected to earth (ungrounded). The outputhas a very low impedance (< 0.1 Ω). The maximum output current is±400 mA.

Order # Product

RM1 73-3713 Stimulator C, 220 VAC, 50 Hz

RM1 73-3716 Stimulator C, 115 VAC, 60 Hz

KEY FEATURESDigital controls

Precise timing accuracy

Compact

Bright display simplifies setup and view of all settings

Monophasic, biphasic and alternating modes

High current output facilitates field stimulation

Stimulator C Specifications

STIMULATION MODE SINGLE: single stimulus triggered by MANUALbutton or by remote contact

FREE RUN: repeating stimulus

GATED: repeating stimulus triggered by MANUAL button or by remote contact

TRIGGER: pulse (TTL, negative-going edge) starts delay circuit

TETANIC: 50 Hz duty cycle square wave. Burst of 2.5 secmaximum duration gated by MANUAL button or remotecontact

STIMULATION TIMINGFREQUENCY

0.01 to 1000 Hz in 42 coarse steps and and continousfine setting with 10 µs resolution respectively

1 ms to 100 s in 41 coarse steps and and continous fine setting with 10 µs resolution

DELAY TIME (leading the stimuli): 0.1 to 500 msec in 32 coarse steps and and continous fine setting with 10 µs resolution

WIDTH 10 µsec to 100 msec in 32 coarse steps and continousfine setting with 2 µs resolution

STIMULUS OUTPUT 1. Output voltage 0 to 50 V; Current ±400 mA (rise andfall time < 1 µs at 50v ±400 mA)

2. Monophasic (Pos or Neg), Biphasic, Alternated

3. Output circuit floating, not connected to earth(ungrounded)

INDICATION 1. LCD display (2 lines of 40 characters each with LED backlight) to indicate stimulus parameters and settings

2. LED for stimulus pulse

3. Beep (to indicate errors in setting)

STIMULUS OUTPUT 1. Output voltage 0 to 50 V; Current ±400 mA (rise andfall time < 1 µs at 50v ±400 mA)

2. Monophasic (Pos or Neg), Biphasic, Alternated

3. Output circuit floating, not connected to earth(ungrounded)

TRIGGER IN/OUTPUT BNC socket Input or Ouput depending on the selected mode.

INPUT SIGNAL TTL level, negative edge or active low

OUTPUT SIGNAL TTL level active low

DIMENSIONS, H x W x W 120 x 240 x 250 mm (4.7 x 9.4 x 9.8 in)


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Stimulator CS for Isolated Cells

The stimulator CS Type 223 is a new development of an electronicstimulator for the electrical stimulation of isolated cells, e.g.myocytes. Stimulating isolated cells needs a high impulse current totrigger a maximum of cells independent of the size, shape speciallyin cell culture medium having a low resistance. This is of importancein experiments where the threshold voltage of cell reaction has to bedetermined. To reduce electrochemistry effects such as polarizationof the electrodes and double layer of gas bubbles biphasicstimulation with perfect symmetry is requested. To be able toactivate an action potential by depolarizing the membrane and toavoid further influence of the stimuli on the cell reaction, short andpowerful impulses are required.

Stimulation Mode and Timing are displayed on a LCD display and can easily be set in a step through mode using the ‘Menu’ button andthe adjust knob. The last setting of the timing is stored in a backupmemory and recalled automatically after switching on theinstrument. The Stimulus Output Voltage is indicated on the built-indigital voltmeter and can be adjusted on two potentiometers:“coarse” and “fine” with adjustment in the range of 0 to 45 V. Theoutput is floating, not connected to earth (ungrounded). The outputhas a very low impedance (less than 1 Ω). To avoid overheating ofthe output circuit a dynamic current limiter is built in. It limits themaximum output current relative to the Stimulation Width.

KEY FEATURESEasy to use

Constant voltage monophasic, biphasic or alternating

stimulation (> 1A)

High output impulse current

Backup memory for timing setting

Specially adapted for isolated cells stimulation

Stimulator CS for Isolated Cells Specifications

STIMULATION MODE SINGLE/STRT single stimulus triggered by START switch or by remote contact

REPEAT/STRT repeating stimulus triggered by STARTswitch or by remote contact

TRIGGER pulse (TTL, negative-going edge) starts delaycircuit; START switch must be in OFF position

TIMING:

STIMULATIONFREQUENCY

0.05 to 100 Hz (in 29 steps) 0.05, 0.1, 0.2, 0.3, 0.4, 0.5,0.6,0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50,60, 70, 80, 90, 100 Hz

DELAY TIME (leading the stimuli): 2 to 50 msec (in 7 steps) 2, 5, 10, 20,30, 40, 50 msec

STIMULUS WIDTH 10 µsec to 10 msec (in 28 steps) 10, 20, 30, 40, 50, 60,70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 µsec, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 msec

STIMULUS OUTPUT 1. Output voltage 0 to 50 V; Pulse Current up to 1 A atmax. pulse width (10 msec)

2. Monophasic, Biphasic, Alternated

3. Output circuit floating, not connected to earth (ungrounded)

INDICATION 1. LCD module (4 lines of 16 characters each) to indicatestimulus parameter settings (except amplitude);

2. Digital voltmeter (DVM) to indicate output voltagesetting range selectable: 0 - 19.99 V <—> 0 - 50.0 V

3. LED for stimulus pulse

4. Beep (with each stimulus pulse), can be switched off

5. 2 LEDs for polarity of next stimulus: + or -)

TRIGGER INPUT/OUTPUT:

INPUT BNC socket, input signal starts time delay, TTL level,negative active edge

OUTPUT BNC socket, signal: delay time, open collector output (2 kΩ to +5V)

WEIGHT 3.5 kg (7.7 lb)

DIMENSIONS, H x W x W 110 x 300 x 250 mm (4.3 x 11.8 x 9.8 in)

Order # Product

RM1 73-0485 Stimulator CS, 220 VAC, 50 Hz

RM1 73-2667 Stimulator CS, 115 VAC, 60 Hz


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Synthetic Scaffold Material

Medical device companies rely on textile building blocks for theirinnovative medical devices. Non-woven bio-textiles have becomethe material of choice for many tissue engineering and regenerativemedicine applications, and with superior surface area, high voidvolume, and excellent permeability, they are now increasingly usedin a wide variety of restorative applications ranging from orthopedicreconstruction and wound management to cosmetic surgery.

How it works:

Composed of felt created by a carding and needle-punch process,non-woven structures provide greater surface area than most otherbiomedical textiles, and bring a different set of benefits toimplantables. Most notably, the ability for customized performanceand controlled absorption profiles. Currently, most device OEMsrequire non-woven scaffolds composed of absorbable biomaterials,meant to facilitate re-growth before disintegration.

Common non-woven applications include:

• Scaffolds for tissue engineering

• Scaffolds for stem cell applications

BIOFELT™ tissue scaffolds can be engineered to a wide variety ofsizes and densities, using PGA, PLA and 90/10. BIOFELT™ can be cutinto disks and squares as small as 2 mm or fabricated as tubes withIDs from 1-10mm. All scaffold products are packaged individually inheat sealed foil bags.

96 well plate with 96 scaffold nibs inserted to use in cell adhesionstudies with your cells 96 well plate16 wells of each type of materialor same material in all 96 well specify when ordering 88-0960Ddifferent materials 88-0960S same material.

Order # Product

RM1 88-0244 Synthetic Scaffold, 1 mm






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Synthetic Scaffold Materials

Aligned 3-D substrates mimic specific human in vivo environments(brain and cardiac tissue) allowing cells to align and orient as they doin vivo which facilitates faster screening and more effective cancerand stem cell research. As a result, researchers are able to moreaccurately study the effects of various chemical compounds on cellbehavior and differentiate stem cells faster and more efficiently. Thisis especially true as researchers attempt to model and measure cellmigration (i.e. metastasis) from the primary tumor or differentiatestem cells into Cardiomyocyte. This technology allows high-throughput testing previously possible only in specialized labs andlive cell imaging through the 3-D nanofibers.

Test cell/scaffold adhesion, perfect recellurization and decellurization protocols, study tissue geometry signaling factors and more.

Works in Multi-well imaging bioreactor (pages 44-45) and HarvardApparatus 3D organ bioreactors.

Randomly Oriented Fibers & Aligned Fibers:Chambers, Dishes and Well Plate Inserts

KEY FEATURESAny diameter, length, or thickness

Choose from a variety of biodegradable and

non-biodegradable polymers

Mechanical properties can be tailored

Shape and design of scaffold can be changed to

reproduce other organs of interest

Order # Product

RM1 88-0212 24-Well Plate, Random Fiber, 700 nm



RM1 88-0215 8 Chamber Slide, Random Fiber, 700 nm

RM1 88-0216 60 mm Dish, Random Fiber, 700 nm

RM1 88-0217 100 mm Dish, Random Fiber, 700 nm

RM1 88-0218 6-Well Plate Inserts (6 inserts), Random Fiber, 700 nm



Aligned Fibers:

RM1 88-0221 24-Well Plate, Aligned Fiber, 700 nm



RM1 88-0224 8 Chamber Slide, Aligned Fiber, 700 nm

RM1 88-0225 60 mm Dish, Aligned Fiber, 700 nm

RM1 88-0226 100 mm Dish, Aligned Fiber, 700 nm

RM1 88-0227 6-Well Plate Inserts (6 inserts), Aligned Fiber, 700 nm



Others:

RM1 88-0230 6 Well IMEMS, 700 nm

Figure 1: Aligned white matter in the human brain. Figure 2: Human brain tumor cells (green) deposited on vertically aligned nanofibers (black).

Nanofiber Blood Vessels and Tracheas

24-Well Plate 96-Well Plate 384-Well Plate

8 Chamber Slide 60 mm Dish 6, 12, 24 WellPlate Inserts

Call 508-893-8999 for Custom Contructs, Blood Vessels, Trachea, etc.


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Lab Site Heparin CoatingApply to Glass and Metals for Medical Devices and Regenerative Medicine Applications

KEY FEATURES:No more blood clotting in bioreactors or clogging

flow circuits

No more scaffold fouling due to clotting

No more loss of proteins due to non-selective binding

to plastics, metals and glass

No more cell aggregation in blood pumping systems

Easy as 1,2,3 to coat your material: PRIME,COAT, RINSE = Long lasting Heparin Coating onTreated Surface

A proprietary heparin conjugate that is a unique surface modificationtool which can be applied to glass, plastic and metal surfaces.Improve the performance of medical device and regenerativemedicine equipment. Lab Site can be used to treat pumps, tubes,stents, beakers, flasks, bioreactors, implants, catheters and more.

Order # Product

RM1 88-0241 Lab Site Heparin Coating Kit+

Each Kit Includes:

RM1 88-0236 PAV Priming Formulation, 125 mg*

RM1 88-0237 CHC Coating Formulation, 25 mg*

RM1 88-0238 Priming Buffer pH 9, 500 ml

RM1 88-0239 Coating Buffer pH 4, 500 ml

RM1 88-0240 Acetylation Buffer pH 10.5, 500 ml

* Supplied as concentrated solution. + Acetic Anhydride must be purchased separately.

Lab Site - mode of action- MIMICS NATURE

Blood vessels present heparin sulphate at the interface towards blood,providing binding sites for antithrombin (AT). Such binding sites servethe purpose of enhancing the anticoagulant effect of AT, and hencethe endothelial surface of the blood vessels is an active tissue thatcontinuously interacts with the circulating blood.

When AT binds to the pentasaccharide sequence in heparin, astructural reorientation is induced in AT which implies that thethrombin binding site of AT becomes more readily available. Thisresults in a thousand-fold increase of the inhibition rate withoutaltering the stoichiometry. Once an irreversible AT-protease complexhas been formed, the affinity between heparin and AT decreases andthe complex may leave the surface and the immobilized heparin isready to attract another AT molecule. This explains the catalytic role ofheparin, which may occur both with soluble heparin, heparinsulphate and heparin adequately immobilized at a solid surface. Itshould be emphasized that CHS™ is not consumed in the process, butmerely acts as a catalyst to potentiate the inhibitory capacity of AT.

Dialysis Buffer OutDialysis

Buffer In

Sample Solution Out

Sample Solution In

SampleChamber

Membrane(1.2 in x 3.2 in)

Dialysis BufferChamber


BIOREACTORS

Now sterilizable, in-line dialysis system to detoxify 3D organbioreactor perfusate. Remove DMSO, salts, and cell signallingmolecules.

The Flow-Thru DIALYZER is a unique system for the rapid dialysis ofsample volumes from 20 µl to 100 ml. It provides a large surfacearea for Flow-Thru on-line dialysis with minimal sample loss. Theentire dialysis unit is made of Teflon, an inert material, and has twoseparate serpentine channels superimposed on each other andseparated by a dialysis membrane. The length of each channel isabout 700 mm. Five different chambers are available (20 µl, 75 µl,150 µl, 300 µl and 600 µl). Chambers of different volumes can alsobe superimposed on each other for specific applications.

Until now, commercially available options for preparing microliter volumeshave suffered major drawbacks including high back pressure, clogging,leakage and limited surface chemistry options. LabSmith’s new CapTitecartridges offer customizable packing and re-use for easy samplepreparation in both prototypes and high-throughput, automated systems.

Filtration or Solid PhaseExtractionIn-Line PEEK cartridge for removal of toxic components

Flow Through Device AccessoriesFor Removal of Toxins, Metabolites or Signaling Products

Flow-Thru DIALYZER™ & Membranes

A. REGENERATED CELLULOSE MEMBRANES

1k Da MWCO 10k Da MWCO



B. CELLULOSE ACETATE MEMBRANES:

100 Da MWCO 10k Da MWCO

500 Da MWCO 25k Da MWCO



5k Da MWCO

C. POLYCARBONATE MEMBRANES:

0.01 µm

0.05 µm

0.60 µm

KEY FEATURESFast

Cost-effective

Preparation of microfluidic volumes

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Polestar Fluorescence-based Biosensors, detectionof pH, CO2 and O2,see page 69-72

Infrared ContrastImaging (IRCI),see page 76-81

Table of Contents

PLUGSYS Modular Measuring Control System 66

PowerLab Data Acquisition System 67-68

Polestar Florescence-based Biosensors 69-72

Universal Perfusion Solution Monitor 73

Handheld Microscopes 74-75

Infrared Contrast Imaging (IRCI) 76

Infrared Contrast Imaging (IRCI) Poster fold out between 76-77

IRCI Cameras 77-79

IRCI Camera Selection Guide 80-81

Infrared Contrast Imaging Guide 82-85

IRCI Imaging Kidney and Lung 86

Visualizing Vascularization Changes 87

Visualization of Vessel and Vascular Leaks During Surgery 88

Visualizing Aerosols 89

Visualization of Water Flow 90

Emissivity, Enhanced Visualization with IRCI 91-93

Visualize with ICI Imaging 94-95


biosensing


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A Range of Harvard Apparatus BIOSENSING Probes areAvailable for Virtually Any Metabolic or PhysiologicalMeasurement

APPLICATION AREASRespiratory Mechanics

Cardiovascular Physiology

Absorption

Metabolism

Excretion

Nutrition

Electrophysiology

Biopotentials

Animal Behavior

Dosing

Vascularization

Tissue Viability

MEASUREMENT PROBESBlood Pressure

Force: Isometric, Isotonic, Auxotonic

Differential Air Pressure

Flow Probes

Capnographs

Pulse Oxymeters

Temperature Monitoring

Thermocouples

ECC/Electrogram

Monophasic Action Potentials

Weight Transducers

Electrical Stimulation Electrodes

Physiological Imaging Devices

Blood Gas Probes: pH, O2, CO

2

Ion & Compound Sensors

- Glucose

- Lactate

- Nitric Oxide

- pH

- Peroxide

- Potassium

- Sodium

Infrared Contrast Imaging

Hand Held Microscopy

Blood Gas Monitoring


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PLUGSYS Modular Measuring & Control System

General System Description

PLUGSYS is a flexible measuring and control system developed foruse in physiological and pharmacological research. Its modulardesign accepts a wide pallet of different plug-in function modulessuch as bridge amplifier, differentiator, integrator, heart rate meter,ECG amplifier etc. All these modules fit directly into the PLUGSYSmain frame, which is available in a 19 inch rack or as a desktop case.

The PLUGSYS main frame is the base of the measuring system. Itconsists of the case, the standard power supply, and the bussystem. The system has 20 slots for PLUGSYS modules, but themaximum number of installable modules in practice depends on thewidth of the module. The PLUGSYS configuration for mostapplications will result in a maximum of 10 to 15 modules per case.

One of the leading features of the PLUGSYS measuring system isthe slot-independent design, that means that all configurationsettings are done on the plug-in modules and not on the system bus.The system can be modified or reconfigured by the using jumpersand dip switches installed on the PLUGSYS modules.

The PLUGSYS is not only a conventional modular analog measuringsystem, it includes specific interface modules and applicationsoftware for data acquisition and analysis.

PLUGSYS System Cases and Minicase

The basic system cases with internal system bus are suppliedcompletely wired and ready for use. The different models haveeither 6, 10 or 20 positions (slot units = SU) which accept thePLUGSYS modules. A MiniCase is also available that has 4 slot unitsand with an optional Case Extension that increases the number to 6slot units.

The Type 601 cases provides a total space of 10 slot units. The Type603 cases provides a total space of 20 slot units. The width of thefunction PLUGSYS modules equals either one or a multiple of it slotunits. For instance, the width of the CFBA Bridge Amplifier is equalto two slot units, therefore it would occupy 2 slot spaces in thePLUGSYS case. The maximum number of PLUGSYS modules —such as a Bridge Amplifier, Differentiator, Integrator, or Heart RateModule — which can be plugged into a unit system case dependson the number of slot units required by each module.

The MiniCase housing is a low-cost and space-saving alternative for1 or 2 modules and allows PLUGSYS modules to be used asconventional stand-alone units. Unlike the PLUGSYS housings Type601 and 603, the MiniCase has no system bus for power supply orsignal link between the individual modules. For this reason, theMiniCase systems are supplied custom wired and configured to suityour requirements. The MiniCase is only suitable for use with certainmodules or module combinations. Please contact Harvard Apparatusfor more information.

KEY FEATURESUniversal modular measuring and controlling

system for recording and data acquisition

Wide range of modules:

– Transducer amplifiers (force, displacement,

pressure, flow)

– Biopotential amplifiers (ECG, EEG, EMG, AP, MAP)

– Amplifiers for biochemical sensors (O2, pH,

ion selective)

– Calculating modules (differentiator, integrator,

heart rate)

– I/O modules (trigger output, recorder output,

trigger input, signal input)

– Controlling and special function modules (ventilator

module, liquid level controller, programmable

stimulator module)

– Interface to recording devices, data acquisition

and controlling

Modular structure permits multi application

platform use:

– 3D Organ Bioreactors

– Hemodynamics

– Pulmonary

– Isolated organ

– Biopotentials

– Combinations (hemodynamics and pulmonary)

Modules can be interconnected internally. There is no

need for external cables which reduces clutter and

confusion. The same is true for recorder and data

acquisition connections. Simple, clutter free

connections reduce the chance of error.

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Call 508-893-8999 for ConfigurationAssistance and Quotation.

T E C H N I C A L S U P P O R T


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PowerLab Data Acquisition Systems with LabChart orLabChart Pro SoftwareADInstruments PowerLab & Harvard Apparatus Your Superior Solution for Data Acquisition

• Atrial & Ventricular Pressure

• Biological Temperature

• Biochemical Sensors

• Blood Pressure – Invasive

• Blood Pressure – Non Invasive

• Blood Flow

• Cardiac Output

• Cardiac Function

• CO2 and NH3

• Conductivity

• Cystometry

• Dissolved O2

• Dose Response

• ECG, EKG

• EEG & ECoG

• EMG

• Evoked Potentials

• Extracellular Recordings

• Gas Analysis

• Gastrointestinal Mobility

• Hemodynamics

• Intracellular Recordings

• Ionic Concentrations

• Isometric Tension

• Isotonic Contraction

• Langendorff Heart

• Membrane Potential

• Microvascular Tension

• Mouse Applications

• Muscle Tissue Contraction

• Neurophysiology

• Nitric Oxide

• Oocyte Voltage Clamping

• Organ Bath Experiments

• Patch and Voltage Clamping

• Perfused Organs

• pH and Ionic Concentrations

• Phenotyping

• Pulse Oximetry

• Respiratory Air Flow

• Sleep

• Sympathetic Nerve Activity

• Telemetry

• Temperature

• Ussing Chamber

• Ventricular Pressure

• Ventricular Pressure-Volume

• Wire Myography

• Working Heart

Harvard Apparatus continues to be theworld leader in measurement ofphysiological signals with anunsurpassed selection of transducers,probes and electrodes for biologicalresearch. Harvard Apparatus also offersthe most comprehensive range of DataAcquisition and Analysis optionsavailable anywhere. From signaldetection, to amplification andconditioning, to digitization and analysis,Harvard Apparatus provides definitiveand complete physiology solutions.

PowerLab data acquisition systems provide the power and versatility to fast track research. The following is a selection of life science researchapplications for which PowerLab systems are commonly used.


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PowerLab Data Acquisition Systems with LabChart orLabChart Pro Software (continued)

The PowerLab Advantage:

PowerLab Data Acquisition Systems are complete data acquisitionand analysis solutions for academic institutions, governmentorganizations and private industry. Supplied with LabChart™ andScope™ software, PowerLab® systems offer comprehensive datarecording, display and analysis features for a wide variety of researchapplications.

Around the PowerLab system we provide a range of amplifiers,instruments and specialized analysis software to provide a completeresearch solution.

LabChart Software

LabChart is suitable for research on any species – from humans tomice to flies. The software provides the capability to continuouslyrecord and display up to 16 channels of data, perform online oroffline calculations, display numerous analysis windows andautomatically extract data. Quick and easy set up of experimentalparameters, powerful computation and analysis features are just thebeginning.

Configuring recording parameters, such as range and filters, takesseconds, with all of the information, including settings, calibrationsand computed values, saved in a single file. Parameters of interestare easily extracted to an internal spreadsheet and can be exportedfor further analysis or graphing.

LabChart Modules

LabChart modules are software add-ons that provide comprehensiveanalysis feature sets for specific research applications. For the latestlist of modules see the softeware updates section of our website.

Hardware

PowerLab systems combine software-controlled input ofparameters, extensive signal conditioning options, variable samplingspeeds and powerful real-time computations with the advantages ofcomputer-based data display and analysis. They offer thefunctionality of a chart recorder, XYT plotter, digital voltmeter andstorage oscilloscope in one compact unit.

PowerLab systems connect to Windows and Macintosh computersusing high-speed USB. Four, eight and sixteen-channel models areavailable with sampling and online computation speeds of up to 200kHz per channel (400 kHz aggregate). They feature independentlyselectable input sensitivities, high-pass, low-pass and anti-aliasingfilters, 16-bit resolution, analog outputs as well as digital input andoutput connections for triggering and controlling external devices.

Signal Conditioners

A wide range of software-controlled preamplifiers is available for usewith PowerLab data acquisition systems. Once connected, the signalconditioners are automatically identified and configured by thePowerLab system with all settings stored when the data file is savedon your computer.

Instruments, Transducers and Accessories

An extensive selection of instruments, transducers and accessoriesis also available to cover numerous life science applications. Third-party products sourced from gold-standard manufacturers are fullycompatible with PowerLab recorders and signal conditioners.

KEY FEATURESProven track record with installations in thousands of

research institutes, universities, hospital and

commercial laboratories around the world.

Power and flexibility to be used in a variety of life science

applications. Start with a PowerLab system and simply

add an appropriate amplifier, instrument or transducer.

Quality and reliability: all products are manufactured

under a quality system certified by an accredited body

as complying with ISO 9001:2000.

Data integrity is paramount. PowerLab data acquisition

systems are calibrated and tested to deliver data you

can trust.

GLP and 21 CFR Part 11 compliance is facilitated when a

PowerLab system is used with the GLP Module and GLP

Server software. Together they provide the required

user interface, audit trail and signing components for

non-repudiation of data.

Intuitive and powerful software allows researchers to

concentrate on the science. With no programming

required, the software is mastered quickly and provides

comprehensive recording, display and analysis features.

Combine with Harvard Apparatus research systems to

create complete solutions for virtually any bio-research

application.

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Polestar Fluorescence-based Biosensors, detection of pH, CO

2and O

2

Harvard Apparatus – Polestar Fluorescence-based Biosensorsrepresent breakthrough technology in the measurement of typicalblood gas parameters in tissue engineering, organ perfusion andgeneral physiology applications.

In contrast to traditional ion-sensitive chemosensors, these opticalbiosensors use a controlled optical source that emits light signals atspecified frequencies which excite an ion-specific sensor spot incontact with the test medium. Changes in the parameter of interestare reported as a proportional change in some characteristic(intensity, phase shift, etc.) of the fluorescence. Because they areinherently self-referencing, this detection technology eliminates thepotential for measurement error arising from changes in sampleproperties (i.e. flow rate, viscosity, etc…) over the duration of anexperiment. This ensures stable, drift-free calibration and hencereliable measurements throughout the lifespan of a sensing element.

KEY FEATURESIdeal for Tissue Engineering and Regenerative

Medicine applications

Completely Sterilizable sensors withstand CIP (Clean-In-

Place), SIP (Steam-In-Place), gamma, and autoclave

conditions

USP Class VI-certified sensors are shipped pre-calibrated

Optical detection technology based on direct

fluorescent measurement

State-of-the-art technology utilizing advanced digital

signal processing electronics

Automatic calibration simplifies operation requirements

Automatic temperature and pressure compensation

ensure measurement accuracy

No reference or electrolyte required

Rugged sensors with stable readings throughout the life

of the sensing element

Dedicated Signal Processor

Automatic Gain Control ensures optimum

measurement reliability

Continuous signal output to any DAQ system

Polestar Flourescence-based Biosensors Specifications

pH

RAPID RESPONSE T90 < 30 sec

DETECTION RANGES 4.7-5.5-10

ACCURACY 0.1

REFERENCE No reference required

CALIBRATION Pre-calibrated or easy 1- or 2- point calibration

O2

SENSOR DETECTION Dissolved or gas-phased


DETECTION RANGES

HIGH LEVEL 0-500% Air, 0-100% Dissolved

MEDIUM LEVEL 0-150% Air

LOW LEVEL 0-20% Air


CO2

SENSOR DETECTION Dissolved or gas-phased


DETECTION RANGE 0-30%

ACCURACY 0.5%

RESOLUTION 0.2


Harvard Apparatus exclusive! Tri-fold inlineflow manifold for one to three sensor typesideal for bioreactor use or other sterileapplications

Unlike polargraphic oxygen electrodes, this technology is non ion-consumptive. These easy-to-use biosensors are free of themaintenance complications inherent in traditional chemosensingrequiring only cleaning and dry storage. There is no need forelectrolyte, membrane changes, or pre-polarization of the electrodeamplifier.

Optical biosensor accuracy, while independent of flow rate, istemperature dependent where the effect is most pronounced withthe O2 and CO2 measurements. In order to ensure the accuracy ofmeasurements regardless of temperature fluctuations, temperatureloss through tubing or other unstable temperature conditions (as canoccur during calibration with gas vs. liquid), the RTD probe is availableto provide a signal to the monitor for compensation of anytemperature-dependent change. The flow through sensors areavailable with a threaded port to accommodate the RTD probe. Fordip-type sensors, the RTD probe can simply be immersed in the fluid.


2and O

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Flow-through sensors with zerodead-volume for integrationwith isolated organ systems.

Standard probes for general lab use

Puncture probes for non-flow smallvolume or soft tissue measurements.

iDot optical bag ports forsingle-use applications

Peel and Stick sensors forplacement within anytranslucent vessel.

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Don't see the right sensor for your application? Contact

Technical Support at 800-272-2775 for Custom Sensor Options

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2and O

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In an in-house study, a CO2sensor (iDOT) was run withcontinuous 3 second samplingduring 0 CO2 phase and 10second sampling during the 4excursions. This study wasperformed in air saturated withdistilled, de-ionized water at 37deg C and periodically checkedagainst certified gas mixturesof CO2. At a 10 secondsampling rate, the total of200,000 samples taken in thisstudy would last 23 days.

Figure 1 Figure 2 Figure 3 Figure 4

Figure 1: After 22K samples Figure 2: After 48K samples Figure 3: After 95K samples

Figure 4: After 200K samples

15% CO2

15%

8% CO2

7.8%

4% CO2

3.7%

15% CO2

15%

8% CO2

7.8%

20% CO2

19.4%

4% CO2

3.8%

15% CO2

14.4%

8% CO2

7.9%

4% CO2

3.7%

15% CO2

14%

8% CO2

7.7%

4% CO2

3.7%

The results shown in the figure (right) illustrate the stability of Polestar’spH measurements over time. During this test, pH measurements weretaken in a phosphate buffer at a 1 second sampling rate for six days.Three sets of pH excursions were conducted in the physiological pHrange to compare Polestar’s sensor performance to a Mettler ToledopH electrode. Polestar’s sensor performance was determined at thebeginning of the test, after approximately 250,000 measurements andagain after more the 500,000 measurements. In the initial excursiontest Polestar’s pH sensor exactly matched the electrode. After 250,000measurements, the Polestar sensor matched the electrode within 0.02.After 500,000 measurements, the Polestar sensor measured between0.04 and 0.07 pH units within the electrode measurement. Followingthe third excursion test a one-point calibration was performed causingPolestar’s sensor to match the standard electrode. The 500,000+ pHmeasurements taken in this test are equivalent to approximately 2months of testing at a 10 second sampling rate measurement.


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Order # Product

STEP ONE - Choose Optical Process Monitor for the appropriatenumber of measurements*:

RM1 88-0140 DSP4000, 1 Channel




*Parameter firmware sold separately.

STEP TWO - Choose Fiber Optic Cables (one per channel in desired length**:

RM1 88-0153 Fiber Optic Cable, Armored, 1 m

RM1 88-0154 Fiber Optic Cable, Armored, 3 m

**5 and 10 meter cables available upon request

STEP THREE - Choose the Data Acquisition Interface Cablecorresponding to the number of measured channels(compatible with Analog BNC input):

RM1 88-0151 DAQ-DSP4000, 1 Channel Interface Cable




STEP FOUR - Choose appropriate power supply:

RM1 88-0149 DSP4000, AC Adapter, 115 V

RM1 88-0193 DSP4000, AC Adapter, 230 V

Order # Product

STEP FIVE - Choose your sensor type and configuration(s):

RM1 88-0136 Tri-Fold Flow Cell, 1 Sensor



RM1 88-0155 Puncture Probe, 24G, O2 Sub-PPB

RM1 88-0156 Puncture Probe, 24G, O2 PPB

RM1 88-0157 Puncture Probe, 24G, O2 PPM

RM1 88-0158 O2 Flowcell, PPM

RM1 88-0139 O2 Flowcell, PPB

RM1 88-0159 pH Flowcell, 5.5-10

RM1 88-0160 CO2 Flowcell

RM1 88-0194 1/16" Tri-Fold Connect Kit



STEP SIX - Choose the firmware that corresponds to your sensors:

RM1 88-0144 O2 Firmware, PPM

RM1 88-0145 pH Firmware, 5.5-10

RM1 88-0146 CO2 Firmware

RM1 88-0147 O2 Firmware, Sub-PPB

RM1 88-0148 O2 Firmware, PPB

STEP SEVEN - Add Temperature Compensation:

RM1 88-0197 RTD Probe for DSP4000, 3 ft

RM1 88-0232 RTD Extension Cable, 10 ftPo

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2and O

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Universal Perfusion Solution MonitorProven electrochemical detection of pH, pO

2, pCO

2in hydrodynamic systems

KEY FEATURESRugged construction

Noise free design, no cross talk

High sensitivity

Good linearity

For studies involving isolated or in situ perfused organs (heart, lung,liver, kidney), it is often important to continuously monitor pO2, pCO2and/or pH of the perfusate or effluate. Which parameter is measureddepends on the organ used and on the type of experiment conducted.

The universal perfusion solution monitor permits precise continuous ordiscontinuous measurement in liquid media of these three keyparameters pO2, pCO2 and pH. The electrodes are all side stream flow-through electrodes and require a pulsation-free roller pump to deliverconstant flow of perfusate through the electrode at flow rates in therange from 0.5 to 2 ml/min. The solution is pulled through each of theelectrodes in series. The pH sensor requires an external referenceelectrode (both the pO2 and pCO2 contain internal referenceelectrodes). A solid state leak free reference system is used. Becauseof the high impedance of these sensors, screening or shielding of themeasuring circuit is required to guard against electrostatic dischargesand other electrical disturbances. The shielding case also provides aconvenient point of attachment to the multi-electrode mounting plate.Sensors are available individually or configured as a complete packagewith suitable amplifiers and data aquisition.

Detail of pH, pO2 & pCO2Sensor System

Detail of pO2 Sensor

Call 508-893-8999 for a custom configuration and full

system quotation.



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Handheld MicroscopesOverview

High Magnification - Up to 200x

Up to 200x of glorious magnification depending onthe distance of the DinoLite from your object.

Connects to USB 2.0

Digitize your microscopy experience allowing youto easily record and share what you’re looking at.

White LED Lighting

Illuminate objects with build in white LED’s. Withbuilt in LED’s, you don’t have to worry about nothaving light.

Measurement and CalibrationCapabilities

Unlock all the features on our DinoCapture andDinoCapture 2.0 software with the ability tomeasure and calibrate measurements.

NaturalColor Technology

With our NaturalColor Technology, the DinoLiteshow natural color respresentation of what yourlooking at. Even in low-lighting situations, theNaturalColor Technology allow images to retainclear and natural look.

MicroTouch II - Convenient andNoticeable On Scope Trigger

Easy to find and easier to take pictures with ourMicroTouch II Technology, you will greatly minimizeblur while taking a picture under magnification thanclicking a button.

Take Pictures and Record Video

For all USB models, you can take pictures as wellas record video directly to your computer.

M WD FOV (x) FOV (y)20 51.7 21.0 15.730 24.1 14.0 10.540 11.0 10.5 7.950 3.8 8.4 6.360 -0.6 7.0 5.2200 -0.4 2.1 1.6210 0.6 2.0 1.5220 1.7 1.9 1.4230 2.8 1.8 1.4

Figure 1: Working Distance Chart; M = magnification rate, WD = working distance, FOV = fieldof view, Unit = mm

The farther away the lowerthe magnification obtained

Approximately 50x and 200xmagnification at touchproximity


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Handheld Microscopes (continued)

Digital Microscopy 0-500X with Integral Illumination: Small, Powerful and Economical

RM1 88-0000

Handheld Microscope

0.3M/Resolution

640 x 480

10~200X Various

Magnification

1/4" Color CMOS

Built-in 4 white LED’s

RM1 88-0026

Handheld Microscope

0.3M/Resolution

640x480

USB 2.0 Output

10~200X Various

Magnification

LED on/off controlled

by software

AM311ST with

Trigger Button

RM1 88-0058

Handheld Iris

Microscope

1.3 MP

USB 2.0 Output

Resolution 1280 x 1024

USB 2.0


10x -20x Various

Magnification

RM1 88-0057

Handheld Iris

Microscope

1.3 MP

Resolution 1280x1024

USB 2.0


500X fixed

Magnification

RM1 88-0051

Handheld Iris Microscope

Pocket sized 3.5" LCD display/image capture

Real time image or video Storage and playback

Up to 200X variable magnification

Built-in 8-Always-On white LEDs lighting

(Option near UV light)

Microscope Resolution 628 x 586

LCD Screen Resolution 320 x 240

RM1 88-0032

Handheld Microscope

0.3M/Resolution 640x480

AV (Video)/TV Output

1/4" Color CMOS


RM1 88-0003, RM1 88-0009

Handheld Microscope

0.3M/Resolution

640 x 480

USB 2.0 Output

10~200X Various

Magnification

Measurement feature

include (*)


RM1 88-0024RM1 88-0053

Handheld Microscope

0.3 MP / 1.3 MP

USB 2.0 Output / or TV

10~50X / 55X~90X

Magnification

Built-in 4/6 white LED’

RM1 88-0054RM1 88-0055RM1 88-0056

Handheld Dental Scope

0.3 MP (TV) 1.3 MP (USB)

USB 2.0 Output / or TV

Various Magnification

Built-in 8 white LED’

Contact us for a systemrecommendation for your particular

application.

T E C H N I C A LS U P P O R T

Infrared Contrast Imaging (IRCI)Advanced Metabolic and Physiological Evaluation of Organisms, Organs, Tissues & Cells

Physiological Fluidics Monitoring

Vascularization/Perfusion

Disease Monitoring with Enhanced Visualization

Infrared Contrast Imaging, (IRCI)…a technology partnership to advance yourability to visualize biological events:

KEY FEATURESDepth Information: Features which may be embedded in

materials are revealed as emission travels through and

out of the material creating apparent effect of being able

to see through material.

Reveal features in materials that have any or all of these

characteristics: composition differences, different surface

coating or finishes, different temperature, different

tonicity, different shapes. These features are revealed as

a feature even if they are not detectable in the visual

spectrum.

Assign a quantitative value to a spatial portion of an

image. Replacing subjective judgments to discriminate

between images.

Provide image enhancement requiring no markers

stains or dyes to visualize otherwise invisible features

an object.

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IRCI Cameras

KEY FEATURESHigh accuracy within temperature ranges

of -20°C up to 900°C

Easy to use - small size

Excellent thermal sensitivity (NETD) of 0.08 K

Exchangeable lenses with 9° FOV and 31° FOV

Real-time thermography with 100 Hz frame rate

USB 2.0 interface, USB powered device

1 m, 5 m or 20 m cable length

Analog input and output, trigger interface

Extremely lightweight (250 g) and rugged (IP65)

Size 45 mm x 45 mm x 62 mm

Diamond Series IR Cameras - Miniature,High-Performace IR Imaging IRCI 9 / IRCI100


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KEY FEATURESBuilt-in LCD Display

2-year warranty

One hand operation

-20 to 250°C range for temperature

Economical IR Camera for MonitoringTemperature i7 / i40 / i50 / i60 and E-Series

KEY FEATURESThermal imaging of 1000 micron area at 3 micron

resolution

IR Microscope, SC-Series

IRCI Cameras (continued)

FLIR i7

FLIR i50

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KEY FEATURESUsability is key. Ideal for visualizing metabolically

inactive areas

Visualize features of tissue and organs with poor visible

contrast now can be visible, display areas of low

vascularization

Built-in video screen

Built-in tripod

USB for easy file transfer

Small, Compact and Powerful by FLIR

IRCI Cameras (continued)

Front

Back

FLIR T300

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Image Order # Product Temp Range Color LCD Touch (Inch)

ImageResolution

Sensitivity(NETD) Lower

the MoreSensitive

The smaller therange the more

sensitive thecamera

The higher thenumber the higher

the resolution

The lower the Mknumber the higher

the sensitivity

Dimond Series

RM1 88-0244 IRCI 9 -20 to 100°C PC-USB 160 x 120 80 mk @ 31°C

RM1 88-0245 IRCI 100 -20 to 100°C PC-USB 160 x 121 80 mk @ 31°C

IB Series

RM1 88-0162 FLIR i7 -20 to 250°C 2.8 120 x 120 100 mk @ +25°C

E Series

RM1 88-0175 FLIR E60BX -20 to 120°C 3.5 320 x 240 50 mk @ +25°C




T Series

RM1 88-0180 FLIR T620 -40 to 650°C 4.3 640 x 481 50 mk @ +30°C

RM1 88-0182 FLIR T360 -20 to 650°C 3.5 640 x 483 50 mk @ +30°C

RM1 88-0183 FLIR T300 -20 to 650°C 3.5 640 x 484 50 mk @ +30°C

RM1 88-0185 FLIR B360 -20 to 120°C 3.5 640 x 486 50 mk @ +30°C

RM1 88-0186 FLIR B300 -20 to 120°C 3.5 640 x 487 50 mk @ +30°C

SC 600 Series

RM1 88-0187 FLIR SC305 -20 to +350°C 320 x 240 50 mk @ +30°C



IRCI Camera Selection Guide

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WaterResistant

ThermalSensistivity

(°C)Battery Life VIS Cam Frame Rate

(Hz)Warranty

(Years) ZoomSW

(QuickReport).

Higher framerate for motion

is better

SW can bepurchasedseparately

Immersable to 30 ft 0.7 PC No 9 2 No Video Maker

Immersable to 30 ft 0.7 PC No 100 2 No Video Maker

Groundbreaking Affordability > Best in Class Performance

Not immersable <0.1 5 No 9 2 No Yes

No other compact Thermal Imaging Camera Comes Close

Not immersable 0.5 3 to 4 3 60 2 1-4x Yes



Not immersable 0.1 3 to 4 3 60 2 1x Yes

Compact IR Camera for Research

Not immersable 0.5 3 5 30 2 1-4x Yes

Not immersable 0.52 3 3.5 30 2 1-4x Yes

Not immersable 0.53 3 3.5 30 2 1-2x No

Not immersable 0.5 3 5 30 2 1-4x Yes

Not immersable 0.5 3 3.1 30 2 1-2x Yes

High Sensitivity Uncooled Infrared Camera for Scientific Applications

Not immersable 0.5 2 No 9 2 No No

Not immersable 0.5 2 No 60 2 No No

Not immersable 0.56 2 No 50, 100, 200 2 No No

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x-ray

ultraviolet

visible

Infrared

radio

Infrared spectrum 0.7 to 1000 micrometers (microns)ELECTROMANETIC SPECTRUM

10-12 10-10 10-8 10-6 10-4 10-2 1 102 104

Wavelength (meters)

700 nm to 20000 nm

ISO 20473 Scheme

DESIGNATION ABBREVIATION WAVELENGTH

Near Infrared NIR 0.78 - 3 µm

Mid Infrared MIR 3 - 50 µm

Far Infrared FIR 50 - 1000 µm

Infrared Contrast Imaging (IRCI) (continued)

What is an IRCI image showing you?

Figure A: The visible picture of what the IR camera is looking at toget the image in C. Since the vessel is under 4 mm of tissue youreye cannot see the embedded vessel or any of the features of thevessel wall inside surface.

Figure B: This shows the actual IR image of a vessel under about 4mm of tissue (no contrast in visible light). The vessel opening, sidewalls of the vessel wall and the tissue surround it and fluid flowinside the tube are very clear with high contrast.

How does it work?

An infrared thermometer measures temperature by detecting theinfrared energy emitted by all materials which are at temperaturesabove absolute zero, (0°Kelvin). This configuration facilitatestemperature measurement from a distance without contact with theobject to be measured. IRCI utilizes the different variables whichcause the emissivities of an object and its contents to be differentwithin a sample and hence provide visible contrast not available inthe visible domain.

Figure C and D: Represent the top of the object being viewed (atthe surface or embedded, respectively and shows those energiescoming to the surface and displays these energies in a chosenpalette of colors to allow for an image to be formed.

Figure D:

1. The surrounding heat in the environment provides heat to the all objects.

2. This heat is absorbed by the object and then the object emits heat emissions.

3. Depending on the composition, thickness, texture, temperature,surface finish of the materials being viewed, the emitted light isbent so, if the emissions have enough energy to reach thesurface, they are observed as contrasted features.

4. The camera can pick up these surface emissions (a shadow ofthe bent emissions) and displays them in the spatial locationthey hit on the array detector in the camera. This creates anemission display in degrees of energy getting to the surface inthe proper spatial location creating the image in C.

Figure A: Visual Image Figure B: IR Camera Figure C: Surface energy Figure D: Embedded energy transfer

The IRCI camera takes advantage of this emitted radiation and

displays the radiation as as spatially accurate emissions

recorder. This image is made up of actual spatial accurate

emission intensities data which show variations in the material.

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Different kinds of materials and gases have different energyemissivities, and will therefore emit IR at different intensities for a giventemperature. The emissivity of a material or gas is a function of itsmolecular structure and surface characteristics. It is not generally afunction of color unless the source of the color is a radically differentsubstance to the main body of material. A practical example of this ismetallic paints which incorporate significant amounts of aluminum.Most paints have the same emissivity irrespective of color, butaluminum has a very different emissivity which will therefore modifythe emissivity of metallized paints.

Just as is the case with visible light, the more highly polished somesurfaces are, the more IR energy the surface will reflect. The surfacecharacteristics of a material will therefore also influence its emissivity.In temperature measurement this is most significant in the case ofinfrared opaque materials which have an inherently low emissivity. Thusa highly polished piece of stainless steel will have a much loweremissivity than the same piece with a rough, machined surface. This isbecause the grooves created by the machining prevent as much of the

IR energy from being reflected. In addition to molecular structure andsurface condition, a third factor affecting the apparent emissivity of amaterial or gas is the wavelength sensitivity of the sensor, known asthe sensor's spectral response. These IR cameras can see bothtemperature differences of materials over large areas depending on thelens and can see at high resolution, depending on # pixels. Sincedifferent materials have characteristics that change the emissivity of thesamples, IR imaging can visualize changes on both temperature andcreate contrasted features in an image by emissivity differences. Thesedifferences allow for monitoring physiological and metabolical changesin a experiment using IRCI imaging. This allows one to see theformation in real time of an cardiac infarct (area of tissue that will have achange emmissivity with reduced vascularization) as shown below.When visible inspection can see little contrast IRCI imaging maybe ableto see it clearly. This is a great advantage for evaluating sites for stemcell drug injections or know where highly vasularized are or not. Our tovisualize tissue changes do to reduced metabolic activity or change indensity or surface coating not able to be seen with visible light.

How does the IR Image see more than your eyes?

Represent quantitative data with color

Every pixel in the camera’s array detector has an emission intensitythat is then converted to an image using colors as an expression ofthe quantitative measurement of the intensity at a given pixel. Sothese images are really the representation of the quantitative valueof the emission data. The choice of a proper palette is importantbecause the pallet can make a feature in the data easier or moredifficult to see. This data can then be displayed and analyzed in anyspreadsheet application.

Figure A: IRCI image of a rat heart in an open chest surgery. This is a normal heart displayingnormal vascularization.

Figure B: Image of heart post infarct. The green and yellow island is the area of reducedvascularization causing an emmissivity change that can be easily be visualized with IRCI.


Visualization of events otherwise difficult to see with visible light imaging alone

Harvard Apparatus in conjunction with FLIR have developed a newway to utilize infrared imaging technology to allow clear visualizationof histological, physiological and metabolic phenomena down to thecellular level. This technique also allows for the visualization ofevents difficult to see in the visible domain, such as physiological andmetabolic changes in live tissue, contrast of physical features withlow visible contrast and visualization of application of therapeuticagents through injection or aerosol. This technology permitsquantitative assessment and viewing of biological events that untilnow have relied on subjective observations. Such increasedobservation and measurement ability can significantly impactdetection of and treatment for abnormal conditions and thus improvethe understanding of low visible contrast biological events byallowing them to be visualized.

Infrared emission is influenced in a predictable way by surfacecomposition, texture, chemical make up, thickness, tonicity andtemperature. These emissions are detected as distinct colorpatterns on the reported image, clearly identifying both physical and

physiological features that cannot be seen using only the visual lightspectrum. We call this new utilization of IR imaging InfraredContrast Imaging- IRCI. Since IRCI utilizes the natural, ambientinfrared emission energies radiated from any object - no fixatives,dyes or stains are used and no excitation signal is required - it isparticularly well suited to sensitive physiological and regenerativemedicine preparations where cells and tissue may be damaged byexposure to contrast agents or certain light frequencies.

IRCI has been employed as a high-sensitivity, relatively low-costmethod in many applications where visible examination reveals littleinformation. Some examples are:

• Visualization of tissue being affected by changes in

vascularization i.e. visualizing cardiac infarct

• Tissue in a bioreactor visualizing tissue and cell confluence

• Visualization of spray coatings and spray coverage.

Emmissivity differences create contrast with IRCI

IRCI has the ability to highlight contrast features by detecting theirinfrared emmissivity, the measurement of infrared light energy givenoff (emitted) from an object. There are many factors which affect anobject’s emmissivity. Changes or enhancement of any of thesevariables will be factors in the detection of physiological, metabolic ormorphological events.

IRCI allows you to see reflections of embedded features on thesurface which allows you to visualize features embedded under tissue.This image clearly shows the hand’s vascular network as though theskin was transparent.

Figure A: Image of hand before using IRCI.

Figure B: Image of hand while using IRCI, vasular network is clearly shown as if skin weretransparent. High resolution images of embedded vasculature can be captured with IRCI.

Figure C: IRCI allows you to see the unseen beneath the skin.

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Utilizing IR emissivity to create high contrast images: Revealing the metabolic, vascular and structural feature of living tissues

In vivo montoring and identification of theboundaries of cardiac infarcts for the purpose of site directed stem cell injection

Using a FLIR infrared camera for Infrared contrast imaging (IRCI) enablesclear visualization of physiological and structural features of living tissuewhich are otherwise very difficult to detect. This is demonstrated in arodent model where cardiac infarct was induced via ligation of the leftanterior descending coronary artery (LAD). Areas of advanced cardiacinfarct generally display a faint and localized “paleness” at the epicenter ofthe infarct, where myocardial injury is greatest and most pronounced. Theimages below clearly show, in a time dependent manner, widespreadchanges in detected emmissivity on the myocardial surface after LADligation. What may be partially visible to an experienced observer using thenaked eye, is now readily apparent to even the uninitiated user of IRCItechnology. As a result, this technique enables routine procedures to bedone without extensive training and with greater accuracy. This techniquehas clear utility in stem cell therapy applications where precise localizationof cells is critical to effective treatment.

Note: Thanks to Dr. Marcello Rota Brigham’s & Woman's Hospital for experimentalassistance. The visual image shows injections being made into a rat heart using theHarvard Apparatus NanoCool Cell injection system. Injection location is guided by theIRCI camera image.

Heart

InfarctArea

Time Zero to Infarct

InfarctArea

Heart

One Minute After Infarct

Heart

InfarctArea

30 Seconds After Infarct

Rat Orientation


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IRCI Imaging: Decellularized Kidney and Lung

IRCI Images ofDecellurized Kidney

A decellurized kidney was imagedduring a recellurization perfusion.The IRCI images show detailedanatomical feature as well as theperfusion path. The IRCI camera hasthe advantage of visualizing about 5mm depth into the decellurizedkidney, with minimal training,methods development andeconomy compared to otherimaging techniques.

IRCI Images ofDecellurized Lung

The decellurized lung was visualizedwith the IRCI camera. The IRCIcamera is imaging surface featuresand down to about 1cm into thetissue revealing features if alveolibeneath the visceral pleurapassages. IRCI is an easy way to see features in a organ that cannot beseen by visible inspection without the use of stains or dyes.

Decellurized Kidney

3D Kidney and Lung Bioreactor

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Decellurized Lung

Note: Harvard Apparatus would like to thank Dr. Daniel Weiss for IRCI imaging assistance.

Decellularized Kidney: Time lapse from perfusion start to 3 minutes of perfusion

Perfusion Path

Perfusion Path


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Visualizing Vascularization Changes

Experiment: Heating pad set at 42°C was placed in joint of the arm.Arm temperature was 34°C Real time images where taken with theIRCI camera.

Description of pictures:

A. The instant heating pad was placed on the skin

B. 10 seconds after heating pad placement

C. 30 Seconds after heating pad placement

The IRCI camera is monitoring in real time the changes in temperature on vascular and tissue levels due to the remote temperature changecaused by the heating pad.

Result:

The IRCI camera can see subtle changes in vascularization temperature,and tissue hydration. For visualization disease states, metabolic activity,or physical features in surface texture and sample density, IRCI providesunique insights.

Experiment: Study the effects of surface modification that have no visible contrast.


Time lapse sequence

A. A tissue that has burn locations above the times.

B. IRCI image of burns at locations above the times

Results:

The burns of 5 and 10 seconds are not visible with the naked eye.The IRCI camera detects the change in emissivity of burned,revealing otherwise invisible tissue changes.

A B C

A B

Visualizing Surface Tissue Damage

5s 10s 15s 5s 10s 15s

Heating Pad


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IRCI image of hematoma-like inclusion forming under 3 mm tissue

Another use of IRCI is the visualization of events occurring beneaththe surface of tissue layers. In Figure A , a tubing section was placed4 mm into a tissue sample. The IRCI camera can visualize the tubingwall structure and flowing liquid with very high resolution eventhough it is embedded in tissue.

Puncturing the tubing from the inside without disturbing the tissuesurface (Figure B) results in clear visualization of the leaking fluid.That is, the hematoma like inclusion of liquid is clearly seen withIRCI. This demonstrates that IRCI can resolve sub-surface events ina completely non invasive manner. Puncturing the tubing from theoutside (i.e. going through the tissue) as in Figure C, produces aneven more obvious leakage plume, similar to what would beobserved during vascular laceration or incision.

Clearly, both surface facing and sub-surface leaks can be seen withhigh resolution using IRCI. This requires no dyes and allows leakdetection in layers without disturbing surface tissues. Harvard Apparatus will be using this technique to enhance the ability ofresearchers to locate vascular abnormalities or specific locations insurgical procedures.

Visualization of Vessel and Vascular Leaks During SurgeryCapture Images in High Resolution

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Figure A: Submerged Vessel

Figure B: Hematoma

Figure C: Blood Leak


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olsApplication of growth factors on regenerated organ scaffolds (as in the

Harvard Apparatus InBreath bioreactor) or spraying drugs on stents, isdifficult without adding visualization dyes and markers to confirmcoverage. On the other hand, use of IRCI cameras is an easy way tovisualize aerosol delivery both in the airborne dispersal pattern and theactual delivered coating on the devices in high resolution and withoutusing any visualization dyes or markers. This is especially important inbiological and medical systems where dyes and markers can representa toxic component to the user or sample.

Visualization of airborne aerosol

IR cameras used as IRCI devices can easily see in real-time, sprays andaerosol dispersal patterns which would not otherwise be clearly visible.This is very useful when working with the Harvard Apparatus NanoCoolCell delivery system. Aerosol content can be visualized on the surfaceto judge coating effectiveness.

Visualize spraypattern fromnozzle in real-time

Harvard Apparatus has utilized FLIR cameras in a way that not onlyprovides temperature imaging but also produces high contrast imagesin biological and medical applications.

This new technique uses the camera’s ability to detect IR emmissivityfrom objects as a function of their surface texture, composition,temperature, shape and more. This new utilization of IR imaging hasbeen called IRCI - Infrared Contrast Imaging. This powerful technologycan visualize with high resolution, experimental conditions based ontissue vascularization, structure or metabolic state.

This enables collection of quantitative data in application areas where ithas not been easily available because of difficulty in visualizingtransparent liquids with minimal visible contrast.

For more information on the uses of IRCI call Harvard Apparatus forinformation on IR imaging. Aerosol detection and coating ideal for stentcoating monitoring, drug spray coating, cell injection monitoring.

Unocoatedtissue withembedded flow channels

Same tissuewith embeddedflow channelbeing coatedwith transparentfluid

Visualizing Aerosols


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Visualization of Water FlowWater flowing into water, visualizing the eddy path and flow paths

Experiemental Question: How can you visualize a transparentsolution continuously flowing into a transparent solution?


Time lapse sequence

A. Bowl with tube on left side about 1/2" under an inch of water

B. Water being pumped into the water for two seconds

C. Water being pump into bowl at 5 seconds

D. Water being pumped into bowl for 8 seconds

Results: The IRCI camera is ideal for visualizing a flow and thedynamics of the flow, in real-time, in liquids. This could be to studythe hydrodynamics of a container or to study the mixing dynamics ofdifferent liquids.

A B

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What is Emissivity?

The emissivity of a material (usually written ε or e) is the relativeability of its surface to emit energy by radiation. It is the ratio ofenergy radiated by a particular material to energy radiated by a blackbody at the same temperature. It is a measure of a material's abilityto radiate absorbed energy. A true black body would have an ε = 1while any real object would have ε < 1. Emissivity is a dimensionlessquantity , so it does not have units. In general, the duller and blackera material is, the closer its emissivity is to 1. The more reflective amaterial is, the lower its emissivity. Highly polished silver has anemissivity of about 0.02.

Emissivity depends on factors such as:

Temperature -Emission angle –Wavelength -Sample thickness -Geometric shapes -Chemical composition

A typical engineering assumption is to assume that a surface'sspectral emissivity and absorptivity do not depend on wavelength, sothat the emissivity is a constant. This is known as the "gray bodyassumption".

Although it is common to discuss the "emissivity of a material" (suchas the emissivity of highly polished silver), the emissivity of amaterial does in general depend on its thickness. The emissivitiesquoted for materials are for samples of infinite thickness (which, inpractice, means samples which are optically thick) — thinnersamples of material will have reduced emissivity.

When dealing with non-black surfaces, the deviations from idealblack body behavior are determined by both the geometricalstructure and the chemical composition, and follow Kirchhoff's law ofthermal radiation: emissivity equals absorptivity (for an object inthermal equilibrium), so that an object that does not absorb allincident light will also emit less radiation than an ideal black body.

Both the intensity of emitted radiation, and the wavelength at which itis most intense, vary with the surface temperature of the emittingbody. Objects at a particular temperature emit radiation over a range ofwavelengths. The intensity of radiation emitted by an object, as afunction of wavelength and surface temperature, can be described by:Q). =A I (A5(e (BO.T) -1)] (1) where Q,. is the intensity of emittedradiation (W) at any particular wavelength, is the wavelength (rn), T isthe surface temperature (K) and A and 8 are constants: 3.742 x108W.IJm4 • m-2 and 1.439 x 104 IJm . K respectively (Plank 1959).Equation (1) generates a series of curves which describe the amountof radiation emitted at each wavelength, for bodies of different surfacetemperatures (Figure 1). The total radiation emitted by a body (Olot)can be obtained by integrating equation 1 across the entire wavelengthrange. The value of this integral is: QIDI =a . T4 (2) where a is theStefan-Boltzmann constant (5.67 x 10.-8 W. m~2 . K~4).

Environmental Physiology of Animals

Pat Willmer et al Blackwell Science LTD

Many biological functions are made uo of lots of steps, the individualcomponents of which cannot easily be measured. In such cases it isnot appropriate to derive an activation energy and instead Q10 valuesare used to describe the effects of temperature on the rate of thefunction being studied. Usually reaction rates are compared at two ormore different temperatures to show how thermally dependent aparticular function is. The Q10 value is then the ratio of the velocitycontstants K1 (at t°C) and K2 (at t + 10°C).

Q10 = K2(t + 10°C /K1(t°C)

or more generally, the ratio between K1 and K2 connected for a 10°Cdifference, i.e.:

Q10 = (K2/K1) (10/t2 - t1)

Emissivity is the Critical Variable to Enhanced Visualization with IRCI

Q10 values for a variety of reactions involved in biological processes

Temperature (°C) Q10

Physical Reactions

Diffusion 20 1.03

Biochemical Reactions

Cytochrome Reductase (possum) >20 1.5

<20 2.5Pyruvate Disease V (Rat) >25 1.7

<25 3.2

Haemoglobin Coagulation 60 13.8

Physiological Reactions

Anemone Oxygen Consumption 5 2.0

15 2.3

Crayfish Heart Rate 5 2.4

15 1.6

25 0.8

Crustacean Gill Movements 5 3.8

Beetle Oxygen Consumption 10 2.4

20 2.1

Insect Thermal Induction of Diapause 20 1.4

10 3.7

Torpid Mammal Oxygen Consumption 20 4.1

Figure 1: Thermal radiation from bodies of different temperatures: the higher the surfacetemperature, the shorter the wavelength and the higher the intensity. Note especially thespectral distribution from the Sun (6000+ °C), from a Sun-heated rock or animal surface at 65°C,and from a mammalian body surface at about 26°C (Dotted line show peak wavelengths.)(Adapted from Hardy 1949; Bond et al. 1967.


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Emissivity is the Critical Variable to Enhanced Visualization with IRCI (continued)

Emissivities and relectrances of various materialsMaterial Emissivity Reflectance (%)Water 0.95Cork 0.95Glass 0.94Wood 0.90Ice 0.92Steel 0.07Aluminium 0.20Copper 0.03Insect Cuticle

Black matt 2-6Black shiny 4-23Pale colors 6-25White 10-35

Small shellDark 2-30White 95

Human skinBlack 5-9White 5-10

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Figure 2: Color effects in the pigeon. Radiant heat reaching the skin under a high insolationregime is greater for black plumage at low wind speeds but greater for while plumage at highwind speeds. (Adapted from Walsberg & King 1978.)

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Utilizing IR for measuring temperature and emmissivity allows for themonitoring of physiological events where chages in vascularizationeffect temperature of the tissues. This technology can be utilized tovisualize tissues and cells where the features are normally of poorvisible contrast. This method of looking at physiological events andseeing poorly visible features more clearly can allow for better:

• Site evaluation assessment of viable or necrotic

areas of tissues

• Observation of morphological features

• Track injection flow paths in real time to evaluate

injection quality

Know more about your experimentwith IRCI imaging

Accurate Noncontact Infrared Measurement For VisualizingPhysiological, Metabolic and Morphological features with IRCI

To assure accurate noncontact infrared temperature measurementkeep in mind the following:

• Distance to Target (Spot) Ratio

• Field of View

• Environmental Conditions

• Ambient Temperatures

• Emissivity

Distance to Target Ratio

The distance controls the energy hitting the detector, the closer thecamera the more signal being picked up.

Field-of-view

The array must have the correct lens in front of it to get as muchillumination of the emitted light onto as many pixels as possible.When choosing a system the lens should be selected to give you theproper working distance and the magnification to display the area ofinterest on as much of the array as possible.

Environmental Conditions

Watch for environmental conditions in the working area. Steam,dust, smoke, etc., can prevent accurate measurement by obstructingthe unit's optics. Dew point or the moisture in the air can causeblurring of the image. Also glass containers or certain plastics willabsorb IR energy rendering them IR opaque. If this happens replacethe material with a IR transparent material if possible.

Ambient Temperatures (the surrounding temperature)

If the thermometer is exposed to abrupt ambient temperaturedifferences of 20 degrees or more, allow it to adjust to the newambient temperature for at least 20 minutes. The camera fixed-mounted sensors are specified for performance within certainambient temperature ranges. For example, for high ambienttemperatures, some camera’s offers air cooling and water coolingoptions as well as accessories, such as Thermojacket.

Emissivity

Emissivity is the measure of an object's ability to emit infraredenergy. Emitted energy indicates the temperature of theobject. Emissivity can have a value from 0 (shiny mirror) to 1.0(blackbody).


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The IR has the ability to contrast objects by their emmissivity, the measurement of energy given off from an object. There are many factorseffecting the emmissivity of an object, so if two objects in the image are at the same temperature but one has a shiny surface the other isrough they will appear as distinct when viewing emmissivity. Below are some of the variables of emissivity that can be manipulated to visualizemetabolic, physiology and morphological features or biological samples.

Emission angle

Differences in the angle from which the emitted energy comes willallow the camera to reveal contrast details.

Surface finish

The surface finish bends the emitted light and can reduce or enhancethe energy to create IR contrast differences.

Sample thickness

The thickness of the sample can affect the emitted energy to create contrast.

Wavelength

The wavelengths have different energy levels And the samples havedifferent absorbance's of these energies, so they can be used tocreate contrast.

Temperature

The camera can be calibrated to accurately measure temperaturedifferences.

Chemical composition

Different materials have different emissivity characteristics. Thisfacilitates contrast enhancement.

Geometric shapes

The angles of emitted energy from different shaped objects cancreate contrast in the IRCI imaging.

gamm

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ultraviolet

visible

Infrared

radio

10-12 10-10 10-8 10-6 10-4 10-2 1 102 104

700 nm to 20000 nm

Rough Smooth

Thick Thin

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Emissivity is the Critical Variable to Enhanced Visualization with IRCI (continued)

Emissivity Differences Create Contrast with IR-CI


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Example of IRCI Imaging& Morphological FeauresIn Vivo Monitoring of an Infarct and Identifing the Non-Infarct

The Harvard Apparatus IRCI camera can allow you to see features thathave little or no visible contrast i.e. area of infarct of the heart. Theimages clearly show the difference in the vasularized areas of the heartversus the damaged areas. Although a trained operator can see somecolor change visibly, the peripheral areas of reduced vascularization arenot visible without the IR imaging. This technique allows routineprocedures to be performed without extensive training and provides theability to see features which are otherwise undetectable.

In Vivo or In Vitro Visualization of Low-Visible-Contrast SamplesVisualization of Vascular Areas

Visible spectrum viewing cannot detect the vascularization that can beseen with the IRCI image. IRCI cannot see through the skin but it cansee the emission on the skin surface as if the skin were

removed. This allows one to see reduced or increased vascularity ormetabolic activity.

IRCI Enhanced Visualization, See What You’ve Been Missing

Rat Orientation

The visible light image appears to be totallyhomogenous emulsions of tissue

The IRCI image clearly shows the heterogenous nature of the tissue

Visualize features in tissue like scar tissue, less metabolically active cells, or physical features

FaceHand Blood Vessels of Hand

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Areas to Inject Stem Cell Treatment

Time Zero to Induced Cardiac Infarct 30 Seconds After Infarct One Minute After Infarct

Suitable Blood Flow NIBPPoor Blood Flow, i.e. Rat Tail Shut Down Coronary Artery Flow

The plume at the end of the needle is the actualfluid being injected into the site of the tissue. Thiscannot be seen visually.

Injection Plume

Suture

Nanomite Needle

Heart

InfarctArea

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Assess Rodent Suitability for Non-InvasiveBlood Pressure (NIBP) Measurements

Ex-Vivo Perfusion Analysis


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Table of Contents

Regenerative Medicine Phases 97-98

Solutions for Regenerative Medicine Applications 99-102

Selected References 103-104

Index 105-108


total solutions & support


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TOTAL SOLUTIONS & SUPPORT

Harvard Apparatus Provides Equipment and Expertise for All Phases of Regenerative Medicine Applications

PHASE 1PREPARATION

& HARVESTINGSurgical preparation, anesthesia

and ventilation, surgical procedures,food and drug delivery, animal

and tissue handling, processing and storage.

PHASE 2ENGINEERING

& MODIFICATIONGene delivery and modification, cell growth, differentiation and

manipulation.

PHASE 3CULTURING & GROWTH

Scaffold preparation, decellularizaton and

recellularization, tissue seeding,scale-up and growth underphysiological conditions.

PHASE 4PHYSIOLOGICAL

ASSESSMENT & CONDITIONMONITORING

Validation of growth-phase and end-stage organ/tissue construct,Monitoring of organ development

for transplant suitability.

TRANSITION

ANIMALORGAN-

TISSUE-CELL

RESEARCH CLINICAL

Equipment& Methodologies

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NEW Systems for Advancing Regenerative Medicine & Tissue Engineering

Phase 1Treatment, Preparation & Harvesting1 Identification Products

➤ Tattoo➤ Clips➤ RFID

2 Hair Removal Products

3 Surgical Equipment➤ Operating Tables & Lights➤ Magnification Products

4 Surgical Instruments➤ Tools➤ Needles➤ Sutures➤ Catheters➤ Sterilization Equipment

5 Anesthesia Equipment

6 Ventilation Equipment

7 Vital-Sign Monitoring➤ Capnographs➤ Homeothermic Blankets➤ Pulse Oximeters➤ Blood Pressure➤ EKG➤ Others

8 Recovery Chambers

9 Infusion Products➤ Drug Delivery➤ Anesthesia Delivery➤ Nutrient Delivery

Phase 2Engineering & Modification

1Cell or Tissue Modification: In Vivo, In Vitro, Gene Delivery

➤ Pneumatic Femto Injectors➤ Electroporation➤ Electrofusion➤ Iontophoresis➤ Liposomes➤ Mechanical Injectors

2 Cell or Tissue Accessories➤ Capillary Tubes, Pullers and Forges➤ Homogenizers➤ Manipulators

3 Perfusion Chambers➤ Imaging Culture Plates

4 Molecular Isolation, Purification Analysis➤ Amino Acid Analysis➤ Electrophoresis➤ Western Blot➤ Protein-Ligand Interactions➤ Protein-Protein Interactions➤ DNA purification Kits

5 Molecular Characterization➤ Spectrometers

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Phase 3Culturing & Tissue Growth1 Cell & Tissue Preparation

2 Cell Growth➤ Media Infusion Pumps

a. physiologicalb. continous flow

➤ Imaging Chambers

3 Cell Sorting➤ Capillary Glass➤ Manipulators

4 Cell Harvesting

5 3D Organ Bioreactors

6 Spinner Flasks

7 Centrifuges

8 Pipetting Systems

9 Tissue Culture

10 Incubators

11 Shakers

12 Continuous flow pumps

Phase 4Physiological Assessment & Condition Monitoring

Cells

1Microscopic Imaging & Perfusion Chambers

2 Microscopic Environmental Controls

3 Electrophysiology Products

4 Condition Monitors

Tissues5 Isolated Tissue & Perfusion Baths

Organs6 Lung Regeneration Bioreactor

7 3D Bioreactors – Hollow Organs

Sensors8 Ion Sensors

Ammonia, Calcium, Chloride, Ethanol,Glucose,.Lactate, Nitrate, Nitric Oxide, Nitrite,Peroxide, Potassium, Sodium, Urea

9 Flow

10 Temperature

11 Pressure

12 Force

13 pH, O2, CO

2


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For Animal, Organ & Cellular Physiologyand Behavioral Research

BEHAVIORAL

Fully integrated research systems covering a range of powerfulbehavioral assays.Publication BH2.

PHYSIOLOGY

Equipment for virtually any Animal, Organ or Cell Biology Experimentfrom animal handling to drug infusion to physiological monitoring.Publication PY2.

For Cell/Tissue Engineering, Imagingand Electrophysiology

Our Harvard Apparatus Family: We have Solutions for all Regenerative Medicine Research ApplicationsWe have Solutions for all Regenerative Medicine Research Applications

TRANSFECTION

A comprehensive line of instruments andaccessories for both electroporation andelectrofusion of mammalian, bacterial, yeast,fungi, insect and plant cells and tissues.Publication BTX1.

CELL MODIFICATION

This guide explains Liposomes, PneumaticInjection, Iontophoresis, Electroporation andMechanical Injectors.Publication CM1.

MICROSCOPIC LIVE CELL:

IMAGING, INJECTION &

ELECTROPHYSIOLOGY

Amplifiers, imaging, micromanipulation andperfusion for the electrophysiological andneurological sciences.Publication W4.

Harvard Apparatus has the largest global support network ofexperts and broadest range of specialized products to assist you in finding the best solutions for your experimental research. Call 508-893-8999 or email [email protected] for technicalsupport or to request a catalog or Technical Application Guide.


Specialized Tools for Model Organisms andEx-Vivo Physiology Systems, like the IH-SRfor Isolated Heart Perfusion

ISOLATED HEART

Detailed system guide for the ultimate ex-vivo perfusion system forsmall rodent heart, the IH-SR.Publication HSE1.

MODEL ORGANISMS

Features products used specifically for smaller organisms includingDrosophila, Nematodes, Xenopus and Zebrafish.Publication MO1.

Specialized Guidesfor Bioresearch

EPITHELIAL TRANSPORT

Discusses the theory of operation andpresents the largest collection of UssingSystems available.Publication WB1.

CELLULAR

ELECTROPHYSIOLOGY TOOLS

Detailed guide for a range of tools for cellbased electrophysiology assays.Publication WB2.

BILAYER WORKSTATION

Focus on the Planar Lipid BilayerWorkstation as a foundation for drugscreening as well as ion channel structure.Publication WB3.

INTEGRATED COMPONENTS

FOR LIVE-CELL IMAGING

Focus on the chambers, perfusion andtemperature control for live cell imaging.Publication WB4.

Our Harvard Apparatus Family: We have Solutions for all Regenerative Medicine Research Applications (continued)



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Advanced Organ, Tissue and CellularEngineering and Therapy Tools for Regenerative Medicine

REGENERATIVE MEDICINE

3D Bioreactors for Lung, Trachea/Bronchus & Blood Vessels. StemCell Injection Systems for Fast Bolus Injection, Pressure-ControlledFluid Delivery, Active Cooling & Mixing of Cell Suspensions forInjection, Electrospinning.Publication RM1.

Smooth High Accuracy Flow,Very High, Very Low orPhysiological Flow Rates

NANO & MICROFLUIDICS, PUMPS & INFUSION

Pulsatile Blood Pumps, Peristaltic Pumps, Syringe Pumps and full line of connectors, tubing and accessories.




Publication PC1.

Publication MF1. Publication IG1.


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Molecular Biology and Assays, Sample Preparation and Separations

1D AND 2D ELECTROPHORESIS, WESTERN BLOT,

IMAGING SYSTEMS, PROTEIN BINDING SYSTEMS, SPE

CLEAN-UP DIALYSIS CLEAN-UP, PCR REAGENTS,

INCUBATORS AND MIXERS

BIOCHROM, Publication B1: Amino Acid Analyzers, Full Family of Spectrometers

BTX, Publication BTX1:The In Vivo and In Vitro Experts in Electroporation and Electrofusion

DENVILLE SCIENTIFIC, Publication DS1: A Complete Family or PCR, Incubators and Mixers

HOEFER, Publication H1::The Electrophoresis People, Molecular Sample Preparation




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Selected ReferencesOur extensive references cover all areas of interest for Regenerative Medicine

Sample Research Publications:

1. Spontaneous hair cell regeneration in the mouse utricle following gentamicin ototoxicity ..BIOREACTOR K Kawamoto, M Izumikawa, LA Beyer, GM … - Hearing Research, 2009 - Elsevier... ifmissing hair cells can be replaced by a regenerative treatment ... Infusion speed was controlled by a syringe pump (Harvard Apparatus Inc., Holliston, MA) at the ...

2. Regenerative medicine bioprocessing: concentration and behaviour of adherent cell …BIOREACTOR BJH Zoro, S Owen, RAL Drake, C Mason, M … - Biotechnology and Bioengineering, 2009 -interscience.wiley.com ... Received March 27, 2009; Accepted April 3, 2009 ... for primary cells typical in regenerative medicine. ... vertically onto a syringe Pump (PHD2000, Harvard Apparatus...

3. Towards microfabricated biohybrid artificial lung modules for chronic respiratory support…BIOREACTOR /Materials KA Burgess, HH Hu, WR Wagner, WJ … - Biomedical Microdevices, 2009 -Springer... 215 McGowan Institute for Regenerative Medicine, University ... Biomed Microdevices (2009) 11:117–127 121 ... PHD 2000 syringe pump (Harvard Apparatus, Holliston, MA ...

4. An automated system for delivery of an unstable transcription factor to hematopoietic stem …BIOREACTOR E Csaszar, G Gavigan, M Ungrin, C Therien, … - Biotechnology and Bioengineering,2009 - interscience.wiley.com... Ontario, Canada 7 McEwen Centre for Regenerative Medicine, University ... ß 2009 Wiley Periodicals, Inc ... a Model 33 Twin Syringe Pump (#553333, Harvard Apparatus, St ...

5. Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cell… BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … Engineering PartC: …, 2010 - liebertonline.com... 16 Issue 3: May 26, 2010 Online Ahead of Print: February 26, 2010 Online Ahead ... Cell SortingAnalysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. ... 1 Hz,4 ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA ...

6. BMI1 sustains human glioblastoma multiforme stem cell renewal…STEM CELL cjb.net [HTML]M Abdouh, S Facchino, W Chatoo, V … - Journal of …, 2009 - neuro.cjb.net... This work revealed thatPcG proteins are required in human GBM to sustain cancer-initiatingstem cell renewal. Materials and Methods. ... The scalp was closed with wound clips (Harvard Apparatus). Animals were followed daily fordevelopment of neurological deficits. ...

7. In vivo multimodal imaging of stem cell transplantation in a rodent model of Parkinson's disease…STEM CELL J Jackson, C Chapon, W Jones, E Hirani, A … - Journal of neuroscience …, 2009- Elsevier... 6-Hydroxydopamine (6-OHDA) was injected into the right striatum at a dose of 16 g, in a volume of 4 L at a rate of 1 L/min, using a micropipettor (Harvard Apparatus, Edenbridge, UK). ...Post-implantation stem cell localisation was assessed using high-resolution MR images. ...

8. Effects of ischemic preconditioning on regenerative capacity of hepatocyte in the …STEM CELL A Bedirli, M Kerem, H Pasaoglu, O Erdem, E … - Journal of Surgical Research, 2005 - Elsevier...model have shown that initiation of the regenerative response depends ... and the common bile duct with a microvascular clamp (Harvard Apparatus, Inc., Hollinston ...

9. Development of microfluidics as endothelial progenitor cell capture technology for …STEM CELL BD Plouffe, T Kniazeva, JE Mayer Jr, SK … - The FASEB Journal, 2009 - FASEB... using a HarvardApparatus PHD 2000 syringe pump (Harvard Apparatus, .23 October 2009 ... EPCs are a potential tool in regenerative medicine and likely ...

10. Substantial detrusor overactivity in conscious spontaneously hypertensive rats with …STEM CELL LH Jin, KE Andersson, YH Kwon - Scandinavian Journal of Urology and Nephrology, 2009 -informaworld.com... 2 Wake Forest Institute for Regenerative Medicine, Wake ... ISSN 1651-2065 online # 2009 Informa UK ... a microinjection pump (PHD22/2000 pump; Harvard Apparatus). ...

11. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELLS/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com...Received 14 February 2009; Accepted 9 December 2009; Published online 7 February 2010. ...analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... Cell fusion by Electrocell manipulator (ECM 2001, BTX Harvard Apparatus) and incubated ...

12. Pilot study to investigate the possibility of cytogenetic and physiological changes in bio- …SCAFFOLD MATERIALS/ electrospinning H Kempski, N Austin, A Roe, S Chatters, SN … - Regen.Med., 2008 - Future Medicine... 3 s -1 (PHD 4400, HARVARD Apparatus Ltd., Edenbridge ... Materials for Tissue Engineering & Regenerative Medicine2,158 ... (2009) Bio-electrospraying embryonic stem cells ...

13. In vivo bone tissue engineering using mesenchymal stem cells on a novel electrospun …SCAFFOLD MATERIALS/ electrospinning M Shin, H Yoshimoto, JP Vacanti - Tissue engineering, 2004- liebertonline.com... Q 5 0.1 mL/min, PHD 2000 syringe pump; Harvard Apparatus, Holliston, MA ... 2009. Nanostructured polymer scaffolds for tissue engineering and regenerative medicine ...

14. Silk fibroin microtubes for blood vessel engineering…SCAFFOLD MATERIALS nih.gov M Lovett, C Cannizzaro, L Daheron, B … - Biomaterials, 2007 - Elsevier... c Center for RegenerativeMedicine, Massachusetts General Hospital, Harvard Medical School ... the bioreactor using a syringe pump (Harvard Apparatus, Holliston, MA ...

15. Quantitative Analysis of Neural Stem Cell Migration and Tracer Clearance in the Rat Brain by MRI…STEM CELL JA Flexman, DJ Cross, LN Tran, T Sasaki, Y Kim, S … - Molecular Imaging and… - Springer... DOI: 10.1007/s11307-010-0311-3 Mol Imaging Biol (2010) ... morphologically transplanted cell migration in the rat brain and parametrically estimate neural stem cell migration speed. ... the skull)using a picoliter syringe pump (Pico Plus Syringe Pump; Harvard Apparatus; Holliston ...

16. Therapeutic effect of genetically engineered mesenchymal stem cells in rat experimental leptomeningeal glioma model. STEM CELL C Gu, S Li, T Tokuyama, N Yokota, H Namba - Cancerletters, 2009 - Elsevier... Mesencult ® murine mesenchymal stem cell medium) after aspiration by 5 ml syringe connected with 23 G needle ... the subarachnoid space by a 50 µl microsyringe (HamiltonCompany, Reno, NV) connected with a 27 G needle and a microinjector (Harvard Apparatus, Inc., South ...

16. Magnetosonoporation: Instant magnetic labeling of stem cells… STEM CELL B Qiu, D Xie, P Walczak, X Li, J Ruiz- … - Magnetic …, 2010 - interscience.wiley.com... VC 2010 Wiley-Liss, Inc ...basal medium (Stemcells Inc., Vancouver, Canada), adding NeuroCult supple- ments for neural stem cell differentiation and ... Using a nanoinjector (Harvard Apparatus, Holliston, MA), the left brain hemisphereswere locally implanted with approximately 8 ...

17. A seeding device for tissue engineered tubular structures BIOREACTOR L Soletti, A Nieponice, J Guan, JJ Stankus, WR Wagner … - Biomaterials, 2006 - Elsevier... The tees were connected to aprecision syringe pump (Harvard Apparatus Inc.,Holliston, MA, USA) outside the chamber by means of hydraulic rotating joints (DeublinCo., Waukegan, IL, USA) and polyvinyl chloride (PVC) tubing. ...

18. A novel culture system shows that stem cells can be grown in 3D and under physiologic pulsatile conditions for tissue engineering of vascular grafts 171.65.102.190….BIOREACTOR[PDF]O Abilez, P Benharash, M Mehrotra, E … - Journal of Surgical …, 2006 - Elsevier... system that would allow future testing of mechanical, biochemical, and thermal stimuli on stem cell differentiation into... (B) Photo of the bioreactor (left) and ... The pulsatile pump was a Harvard Apparatus Model 1405 (Harvard Apparatus, Holliston, MA) modified for computer control...

APPLICATION GUIDESBilayer Workstation

Bolus Injections

Cell Infusion

Electrode

Culture Dish Feeding

Drug Injections

Electrospinning Scaffolds

Imaging

Isolated Heart System

Nanofluidics

Stem Cell Injections

Stem Cell Collection and Harvesting

Ussing Chamber

Ventilating Animals


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19. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization…BIOREACTOR MLovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation,and ...Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

20. A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle–Derived Stem Cells BIOREACTOR KC Clause, JP Tinney, LJ Liu, B … - … EngineeringPart C: …, 2010 - liebertonline.com... Activated Cell Sorting Analysis of Unsorted Muscle-Derived Stem Cells and Muscle-Derived Stem Cell-3DGB. MDSC-3D collagen gel bioreactor (MDSC-3DGB) construction... 1 Hz, 4 ms, 50–100 V, rectangular pulses) using a stimulator (Harvard Apparatus, Holliston, MA). ...

21. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLDMATERIAL F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte,and mesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

22. Fabrication and Characterization of Prosurvival Growth Factor Releasing, Anisotropic Scaffolds for Enhanced Mesenchymal Stem Cell Survival/Growth and Orientation …SCAFFOLDMATERIALS F Wang, Z Li, K Tamama, CK Sen, J Guan - Biomacromolecules, 2009 - ACS Publications... It has been shown to enhance survival of many cell types including smooth muscle cell, cardiomyocyte,and mesenchymal stem cell.(21-23) Besides, IGF-1 ... The gelatin/BSA/IGF-1 solution was fed at a rate of 0.2 mL/h by syringe pump A (Harvard Apparatus) into the inner tubing. ...

23. Porous nanocrystalline silicon membranes as highly permeable and molecularly thin substrates for cell culture …SCAFFOLD MATERIAL AA Agrawal, BJ Nehilla, KV Reisig, TR Gaborski, DZ… - Biomaterials, 2010 - Elsevier... Received 8 March 2010; accepted 16 March 2010. Available online 15 April 2010. Abstract. ...An open perfusion microincubator (Harvard Apparatus) was used to maintaincells at 37 °C and mineral oil was floated on top of the media to minimize evaporation. ...

24. A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning… SCAFFOLD MATERIAL nih.gov [HTML]F Teulé, AR Cooper, WA Furin, D Bittencourt, EL … -Nature protocols, 2009 - nature.com... Bacteria have quicker generation times and do not require a very sophisticated laboratory setup,thus reducing time and expenses. Additionally, the production can beeasily scaled-up using bioreactors. ... no. P-259); Harvard Pump 11 plus single syringe (Harvard Apparatus, cat. ...

25. Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials …SCAFFOLD MATERIALnisco.ch [PDF]SN Jayasinghe, N Suter - Biomicrofluidics, 2010 - link.aip.org... Biomicrofluidics 4, 014106 (2010)]. ... glass microslides, while for scanning electron microscopy (SEM) and transmission electronmicroscopy (TEM), fibers were collected ... at the lowest possible flow rate allowed by our present syringe pump, (PHD4400, Harvard Apparatus Ltd., Kent ...

26. Characterization and tensile strength of HPC-PEO composite fibers produced by electrospinning …SCAFFOLD MATERIAL L Francis, A Balakrishnan, KP Sanosh, E Marsano - Materials Letters,2010 - Elsevier ... strength of HPC-PEO composite fibers produced by electrospinning, Materials Letters (2010), doi: 10.1016/j ... PEO, Sigma Aldrich, Mw = 10,000) were used as starting precursors to fabricatenano fibers. ... of 250 m and was mounted on a syringe pump (Harvard Apparatus serial n A ...

27. Tbx3 improves the germ-line competency of induced pluripotent stem cells..STEM CELL/ Electroporation J Han, P Yuan, H Yang, J Zhang, BS Soh, P Li, SL Lim, … - Nature, 2010 - nature.com ...karyotype analyses, Y. Loon Lee, P. Gaughwin and colleagues from the Stem Cell and Developmental ... h, inactivated feeder cells and fresh media were added, and the culture was then ...2-cell fusion by Electrocell manipulator (ECM 2001, BTX Harvard Apparatus) and incubated ...

28. Osteochondral Interface Tissue Engineering Using Macroscopic Gradients of Bioactive Signals …STEM CELLS NH Dormer, M Singh, L Wang, CJ Berkland, MS … - Annals of biomedical …,2010 - Springer ... 0090-6964/10/0600-2167/0 © 2010 Biomedical Engineering Society ... There is a growing interest in hUCMSCs as a mesenchymal stem cell source, as they have been ... in a con- trolledmanner using programmable syringe pumps (PHD 22/2000, Harvard Apparatus, Inc., Holliston ...

29. Engineered Early Embryonic Cardiac Tissue Increases Cardiomyocyte Proliferation by Cyclic Mechanical Stretch via p38-MAP Kinase Phosphorylation …BIOREACTOR nih.gov[HTML]KC Clause, JP Tinney, LJ Liu, BB Keller, … - … Engineering Part A, 2009 - liebertonline.com ... 1 Hz, 4 ms, and 70–100 V) using a stimulator (Harvard Apparatus, Holliston, MA). ... Pasumarthi, KB,Soonpaa, MH, and Field, LJ Myocyte and myogenic stem cell transplantation in ... A Three-Dimensional Gel Bioreactor for Assessment of Cardiomyocyte Induction in Skeletal Muscle ...

30. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CCAnamelechi, EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation forthree-dimensional polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

31. Dynamic Seeding of Perfusing Human Umbilical Vein Endothelial Cells (HUVECs) onto Dual-Function Cell Adhesion Ligands: Arg-Gly-Asp (RGD)− Streptavidin and …BIOREACTOR CCAnamelechi, EC Clermont, MT Novak, WM … - Langmuir, 2009 - ACS Publications... combined a dynamic seeding system with a bioreactor proliferation phase to increase cellular adhesion and proliferation forthree-dimensional polymer ... The dynamic seeding system consisted of a 10 mL syringe mounted on a Harvard Apparatus pump, infusing cells onto Teflon ...

32. Regeneration and orthotopic transplantation of a bioartificial lung-BIOREACTOR HC Ott, B Clippinger, C Conrad, C Schuetz, I … - Nature Medicine, 2010 - nature.com ... Received 22 December2009 Accepted 04 July 2010 Published online 13 July 2010 ... black arrowheads), axial (gray arrowheads) and septal (black arrows) elastic fibers in decellularized ...trachea and was placed in a sterile, water-jacketed organ chamber (Harvard Apparatus). ...

33. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org …BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006... 1. Alow-magnification micrograph of an entire well is shown in Figure 5. Also on post-seeding day 1, the cell culture medium was ... These syringes were loaded into a programmable pump capable of supporting 10individual syringes (PHD2000, Harvard Apparatus, Holliston, MA ... Cited by 53 - Related articles - All 9 versions

34. … Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and Prevascularization …BIOREACTORS MLovett, D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem CellDifferentiation, and ... the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA ...

35. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett,D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... tube embedded within the collagen gel using a programmable syringe pump (Remote PHD Push/Pull ProgrammablePump; Harvard Apparatus, Holliston, MA). For oxygen measurements,a custom housing for the bioreactor and oxygen measurement probe was ...

36. Cardiac tissue engineering using perfusion bioreactor systems …BIOREACTOR nih.gov [HTML]M Radisic, A Marsano, R Maidhof, Y Wang, G … - Nature protocols, 2008 - nature.com... only withinthe construct, that is, at the construct outlet oxygen concentration stops varying as a function of bioreactor length ... 97063A134), coated with PTFE by Microsurfaces; Stereomicroscope; Sterilization pouches;Syringe pump (Push/Pull; Harvard Apparatus or WPI Instruments ...

37. Simple Modular Bioreactors for Tissue Engineering: A System for Characterization of Oxygen Gradients, Human Mesenchymal Stem Cell Differentiation, and … BIOREACTOR M Lovett,D Rockwood, A Baryshyan, DL … - … Engineering Part C: …, 2010 - liebertonline.com... Medford, MA 02155. E-mail: Received: April 19, 2010 Accepted: June 1, 2010. ... 4 µL/min) of the silk tube embeddedwithin the collagen gel using a programmable syringe pump (Remote PHD Push/Pull Programmable Pump; Harvard Apparatus, Holliston, MA). ...

38. Distribution of NTPDase5 and NTPDase6 and the regulation of P2Y receptor signalling in the rat cochlea nih.gov …BIOREACTOR [HTML]MG O'Keeffe, PR Thorne, GD Housley, SC Robson, …- Purinergic …, 2010 - Springer... Purinergic Signalling (2010) 6:249–261 251 Page 4. ... Plastic tubing was placed inside the inlet and outlet holes and both tubes were connected to separate Hamilton syringessecured on a push–pull syringe pump (Harvard Apparatus, USA). ...

39. Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes sbi.org…BIOREACTOR [PDF]BJ Kane, MJ Zinner, ML Yarmush, M Toner - Anal. Chem, 2006 -Thesesyringes were loaded into a programmable pump capable of supporting 10 individual syringes (PHD2000, Harvard Apparatus, Holliston, MA). ... demonstrated that cocultured primary hepatocytes and fibroblastsin a flat plate bioreactor were not adversely effected by wall ...

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Regenerative Medicine Catalog Index

Product Page

% Concentration Mixer............................................................................133-Axis Imaging Platform ..........................................................................433D Organ Reactors

Single Organ ....................................................................................34-43Multiwell ..........................................................................................44-53Hollow Organs..................................................................................37-40

Arteries ....................................................................................37-40Cartilage ..................................................................................37-40Intestines ................................................................................37-40Lung ..........................................................................................37-40Trachea ....................................................................................37-40

Solid Organs ....................................................................................39-42Kidney ............................................................................................41Ligaments ......................................................................................42Linear Stress & Relation Bioreactor ........................................42Liver................................................................................................41Muscles ........................................................................................42

4 Simultaneous Perfusing Imaging Bioreactors ..........................34-4312-Well Imaging Bioreator ................................................................34-4324-Well Imaging Bioreator ................................................................34-4348-Well Imaging Bioreator ................................................................34-4396-Well Imaging Bioreator ................................................................34-43

AAmino Acid Analyzer ..................................Request Biochrom CatalogAcquisition & Control Systems ........................................................66-68

PLUGSYS ................................................................................................66PowerLab..........................................................................................67-68

Aerosol Visualization ..................................................................80-81, 89Anesthesia Systems................................96-102, Request Catalog (PY2)Animal Infusion Pumps ..........................................................17-20, 25-26Applications

Animal Infusion Pumps ......................................................17-20, 25-26Biosensors........................................................................................64-95Bioreactors ......................................................................................34-59Blood Gas Monitoring ....................................................................69-72Compositional Blending ......................................................................13Contrast Imaging (IRCI) ..................................................................76-95Cooled Stem Cell Injectors ..............................................................9-12Data Acquisition ..............................................................................66-68Decellurization ......................................................................................54Electrospinning Pumps ........................................................................14Hand-Held Injection ..........................................................................9-12Metabolic Imaging ....................................................................80-81, 87Microfluidics Pump ........................................................................17-20Organ Perfusion ..............................................................................23-24Organ Regeneration........................................................................34-59Physiological Imaging ..............................................................80-81, 87Perfusion Pumps................................................................................9-33Recellurization ................................................................................34-59Sensors..................................................................................43-53, 74-95Small Gauge Needles ........................96-102, Request Catalog (PY2)Stem Cell Injection ............................................................................9-12Stereotaxic Injection ........................................................................9-12Zero Dead Volume Injection ..............................................................11

Product Page

Arteries Bioreactors ..........................................................................39-42Autoclips......................................................96-102, Request Catalog PY2

BBehavioral Products ....................99, Request a Panlab Catalog (BH1)

Coulbourn Instruments Catalog (CB1) Bibliographies..................................................................................103-104Biomaterials ........................................................................................60-62Biphasic Stimulator ............................................................................55-57Bioreactors ..........................................................................................34-59

3D Organ Reactors..........................................................................34-59Single Organ ....................................................................................34-59Multiwell ..........................................................................................42-59

Bioreactors by OrganHollow Organs..................................................................................37-40

Arteries ....................................................................................37-40Cartilage ..................................................................................76-77Intestines ................................................................................37-40Lung ..........................................................................................37-40Trachea ....................................................................................37-40

Solid Organs ....................................................................................41-42Kidney ............................................................................................41Ligaments ......................................................................................42Linear Stress & Relaxation Bioreactor ....................................42Liver ..........................................................................................41-43Muscles ........................................................................................42Skin ................................................................................................42

Biosensors ............................................................................................64-95Blood Pressure ....................................96-102, Request Catalog (PY2)Capnographs ........................................96-102, Request Catalog (PY2)CO2 ..........................................................81-84, Request Catalog (PY2)Confluence ..........................................................................86-109, 53-65Flow ......................................................96-102, Request Catalog (PY2)Force......................................................96-102, Request Catalog (PY2)O2 ..............................................................81-84, Request Catalog (PY2)Ph ............................................................81-84, Request Catalog (PY2)Pressure................................................96-102, Request Catalog (PY2)Temperature ........................................96-102, Request Catalog (PY2)

Biphasic Stimulation ..........................................................................55-59Blood Gas..............................................................................................69-72Blood Pump ..........................................................................................21-22Blood Pressure ........................................96-102, Request Catalog (PY2)Bolus Injection ..........................................................................................16Burns ..........................................................................................................87

CCameras ................................................................................................74-95

Visual Digital Microscopes............................................................74-75Infrared Camera ..............................................................................76-95

Camera Positioning Platform ............................................................43-45Capnographs ............................................96-102, Request Catalog (PY2)Cell Biology........................................99, Request Warner Catalog (W4)Cell Bioreactor ....................................................................................44-53


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Cell Culture Equipment ..........................Request Denville Catalog (D1)Cell Culture Perfusion Pumps ................................................................15Cell Culture Stimulator ......................................................................55-59Cell Friendly Pumps............................................................................23, 33Cell Injection System............................................................................9-11Cell Therapy Injection Systems..........................................................9-11Centrifugal Pump ......................................................................................23Coating Heparin ........................................................................................62Clot Reducing Coating ............................................................................62CO2 Microscope Stage Incubator ....................................................44-54CO2 Sensor ............................................................................................69-72Complex Mixture Delivery ......................................................................13Confluence Detection ..................................................................43, 74-95Contrast Imaging..................................................................................76-95Connectors Tubing ....................................................................................29Contact Information ....................................................Inside Back CoverCooled Stem Cell Injectors..................................................................9-11

DData Acquisition ..................................................................................66-68Decellurization Manifold ........................................................................54Dialyzer Flow Through ............................................................................63Digital Gas Mixer ................................................................................51-53Digital Microscope ............................................................................74-75Dual In-Line Solution Heater/Cooler ....................................................30

EElectric CO2 Microscope Stage Incubator ....................................44-54Electrical Stimulation

Cell Culture EP Stimulator..............................................................55-57Stimulator-C for Organs and Tissues ................................................58Stimulator-CS for Isolated Cells ........................................................59

Electrophysiology....................................96-102, Request Catalog (PY2)Electroporation ....................................99, Request BTX Catalog (BTX1)Electrofusion ........................................99, Request BTX Catalog (BTX1)Electrophoresis ..........................................Request Hoefer Catalog (H1)Electrospinning Pump ..............................................................................14Embolism Visualization ................................................................80-81, 88

FFast Bolus Injector for Cell Therapy ....................................................16Flow Sensor..............................................96-102, Request Catalog (PY2)Flow Through Dialysis Accessories ....................................................63Fluid Control System

Buffer ....................................................................................12-22, 25-29Media ....................................................................................12-22, 25-29Perfuse/Inject ......................................................................12-22, 25-29Ventilation ............................................96-102, Request Catalog (PY2)

Fluorescence-Based pH, CO2, O2 Biosensors ................................69-71Force Transducer ....................................96-102, Request Catalog (PY2)

Product Page

GGas Mixer..............................................................................................51-53Gradient Solution System ........................................................................13

HHair Removal ............................................96-102, Request Catalog (PY2)Hand-Held Injector................................................................................9-11Hand-Held Pump....................................................................................9-11Hand-Held Pump Cooled ......................................................................9-11Harvard Apparatus Advantage ................................................................6Harvard Bioscience Companies ..........................................................4-5Heparin Coating ........................................................................................62High Pressure Injections Pump..............................................................20Hollow Organ Bioreactor ..................................................................37-38Homeothermic Blanket ..........................96-102, Request Catalog (PY2)Hydrostatic Perfusion Chamber Decellurization ..........................74-95

IImaging

Infrared Camera ..............................................................................76-95Aerosol Visualization ......................................................80-81, 89Blood Source Leak Detection ........................................80-81, 88Burn Assessment ............................................................80-81, 87Embolism Visualization ....................................................80-81, 88Hydrodynamic Mixing Visualization..............................80-81, 90Hematoma Visualization..................................................80-81, 88Infarct Visualization ............................................80-81, 83, 85, 87Metabolic Activity Visualization ....................................80-81, 87Organ Perfusion Visualization..................................80-81, 86, 95Vascularization Visualization..........................................80-81, 87

Visual Microscopes5x-40X Magnification............................................................................7410X-200X Magnification ......................................................................7420X-200X Magnification ......................................................................74400X-470X Magnification ....................................................................74500X Magnification ..............................................................................74Microscope Eyepiece ..........................................................................74Camera Positioning Platform ..............................................................43

Imaging Bioreactor ............................................................................43-53InBreath 3D Organ Bioreactor ..........................................................37-38Incubator

Microscope Stage ........................99, Request Warner Catalog (W4)Table Top ..............................................Request Denville Catalog (D1)

Infrared Imaging Lung ..............................................................................86Injector into Cells-PDMI ........................96-102, Request Catalog (PY2)Intestine 3D Organ Bioreactor ..........................................................37-38Imaging Perfusion Chambers ........99, Request Warner Catalog (W4)Imaging Multi-Well Bioreactor ........................................................44-54IRCI Contrast Imaging ........................................................................76-95

KB-2 Kidney 3D Organ Bioreactor Porcine ................................41-51Isolated Organ Baths ............................Request a HSE Catalog (HSE1)



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KKidney 3D Organ Bioreactor ..................................................................41LB-2 3D Bioreactor Rat Mouse Lung ..............................................39-40

LLigaments ..................................................................................................42Linear Stress Bioreactor ........................................................................42Liquid Sensors......................................................................................81-83Liver ............................................................................................................41LVB-3 3D Bioreactor Porcine Liver ..................................................69-71Lung 3D Organ Bioreactor ................................................................39-40

MMCP Pump Drive ......................................................................................25MCP Pump Head ..................................................................................26-28Metabolic Imaging Systems..............................................................74-95Microdialysis ..........................................Request CMA Catalog (CMA1)Microfluidic Pump ..............................................................................17-20Microscopes ..............................................................................................74

5X-40X Magnification ..........................................................................7410X-200X Magnification ......................................................................7420X-200X Magnification ......................................................................74400X-470X Magnification ....................................................................74500X Magnification ..............................................................................74Microscope Eyepiece ..........................................................................74

Microscope Cameras Eyepiece ............................................................74Multi-Well Imaging Bioreactor ........................................................44-53

1-, 2-, 4-, 6-, 12-, 24-, 48-, 96-Well Templates..............................45-46

N-ONanoCool ................................................................................................9-11Optical Loops ..........................................96-102, Request Catalog (PY2)Ordering Information ..................................................Inside Back CoverO2 Sensor ..............................................................................................69-72Organ Bioreactors ..............................................................................34-41Organ Perfusion ..................................................................................23-24Organ Regeneration ............................................................................34-59

PPCR ............................................................Request Denville Catalog (D1)Peristaltic Pumps ................................................................................24-25Pump Heads..........................................................................................26-28Perfusing Organs ................................................................................34-41Perfusion Pumps..................................................................................12-33pH, CO2, O2 Perfusion Monitors ........................................................69-72Physiological Imaging..................................................................80-81, 87Pressure Controlled Infusion Pump ......................................................12Pressure Sensor ......................................96-102, Request Catalog (PY2)

Product Page

Push-Pull Pump, Continuous Flow ........................................................15Posters

Trachea..............................................................fold out between 40-41Infrared Imaging Infarct ................................fold out between 76-77Infrared Imaging Kidney ................................fold out between 40-41Lung....................................................................fold out between 40-41Cooled Stem Cell Injector ..................................fold out between 8-9Trachea & Bronchi ..........................................fold out between 40-41X,Y,Z Imaging Bioreactor Platform................fold out between 44-45

PowerLab ..............................................................................................67-69Pressure Controlled Injection Systems ................................................12Pressure Sensors ....................................96-102, Request Catalog (PY2)Pulsatile Blood Pump ........................................................................21-22PLUGSYS ....................................................................................................66Pumps ....................................................................................................12-33

Syringe Pump ....................................................................................9-20Advanced Programmable ....................................................17-20% Concentration Gradient..........................................................13Cell Injection..............................................................................9-11Continuous Flow ..........................................................................15Cooled Cell Injection................................................................9-11Electrospinning ............................................................................14Fast Bolus Injectors ....................................................................16Hand Held Injector ..................................................................9-11High Pressure Injection ..............................................................20Pressure Controlled Flow ..........................................................12Pulsatile Blood ........................................................................21-22Stereotaxic Injector ................................................................9-11

Centrifugal ..................................................................................23-29, 33Cell Perfusion Gentle ............................................................23, 33

Peristaltic..........................................................................................24-25Cell Gentle ........................................................................25-26, 28Traditional ..........................................................................25-26, 29

Pump Accessories ..............................................................................29-30Pump Guide ................................................................................................31Pump Theory ........................................................................................32-33Puncture Probes, pH1 O2 CO2 ................................................43-53, 74-95

RReactor Acquisition & Control System ..........................................66-68Reactor Vessel ....................................................................................34-59Recellurization Chambers ......................................................................54References ........................................................................................103-104Respiratory Monitor ................................96-102, Request Catalog (PY2)

SScaffolds ..............................................................................................60-61

Synthetic ..........................................................................................60-61Sensors ......................................................................................43-53, 74-95

Blood Pressure ....................................96-102, Request Catalog (PY2)Capnographs ........................................96-102, Request Catalog (PY2)CO2 ..........................................................................................43-53, 74-95Confluence........................................................................................77-95


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Sensors (continued)Flow........................................................96-102, Request Catalog (PY2)Force......................................................96-102, Request Catalog (PY2)Metabolic Imaging ....................................................................80-81, 87O2 ............................................................................................43-53, 74-95pH............................................................................................43-53, 74-95Pressure................................................96-102, Request Catalog (PY2)Temperature ........................................96-102, Request Catalog (PY2)

Small Gauge Needles ..............................................................................11Solid Organs ........................................................................................39-42Solution Heaters & Coolers In-line ......................................................30Spectrophotometers ........................Request a Biochrom Catalog (B1) Stage Incubator....................................................................................34-43Stem Cell Injection ..................................................................................11Sterilized Sensors

CO2......................................................................................................69-73O2 ........................................................................................................69-73pH ......................................................................................................69-73

Stereotaxic Drug & Stem Cell Injectors ..........................................9-11Stimulators

Cell Culture EP Stimulator..............................................................55-57Stimulator-C for Organs and Tissues ................................................58Stimulator-CS For Isolated Cells ........................................................59

Surgical EquipmentAnesthesia Circuits ............................96-102, Request Catalog (PY2)Autoclips ..............................................96-102, Request Catalog (PY2)Homeothermic Blankets ....................96-102, Request Catalog (PY2)Lights ....................................................96-102, Request Catalog (PY2)Digital Microscopes............................96-102, Request Catalog (PY2)Optical Loops ......................................96-102, Request Catalog (PY2)Sutures ..................................................96-102, Request Catalog (PY2)Syringes ................................................96-102, Request Catalog (PY2)Tables ....................................................96-102, Request Catalog (PY2)Tools ......................................................96-102, Request Catalog (PY2)Ventilators ............................................96-102, Request Catalog (PY2)

Syringe Heaters and Coolers ..................................................................30Syringe Pumps ..................................101, Request Pump Catalog (PC1)Synthetic Scaffold Materials ............................................................60-61

TTable-Top Incubator ..................96-102, Request Denville Catalog (D1)Tattoo Equipment ....................................96-102, Request Catalog (PY2)Temperature Sensors ..........................................................................76-95Technical Support ........................................................508-893-8999 (US)Trachea 3D Organ Bioreators............................................................37-38Tubing & Connectors ..............................96-102, Request Catalog (PY2)

U-VULTRA-Microliter Injection System ......................................................11Ventilators ................................................96-102, Request Catalog (PY2)Visual Digital Microscopes ..............................................................74-75

Product Page

Visualization Cameras ........................................................................74-95Infrared..............................................................................................74-95Visual Digital Microscopy ..............................................................74-95

Vital Sign Monitoring ............................96-102, Request Catalog (PY2)

W-ZWaste Mangement ..............................................................................63-73

CO2......................................................................................................69-71Filtration..................................................................................................63O2 ........................................................................................................69-73O2 ........................................................................................................69-73Dialysis....................................................................................................63

Western Blot ..........................................Request a Hoefer Catalog (H1)What’s NEW ..............................................................................................1-2X,Y,Z, Bioreactor Imaging Platform ......................................................43Zero Dead Volume Needles ....................................................................11


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UNITED STATES Harvard Apparatus84 October Hill RoadHolliston, Massachusetts 01746, USAphone 508.893.8999toll free 800.272.2775 (USA Only)fax 508.429.5732e-mail [email protected] www.harvardapparatus.com

Warner Instruments1125 Dixwell AvenueHamden, Connecticut 06514, USAphone 203.776.0664toll free 800.599.4203 (USA Only)fax 203.776.1278e-mail [email protected] www.warneronline.com

Coulbourn InstrumentsAttn: Sales Department5583 Roosevelt StreetWhitehall, Pennsylvania 18052, USAphone 610.395.3771fax 610.391.1333e-mail [email protected] www.coulbourn.com

CANADA Harvard Apparatus CanadaAttn: Sales Department6010 Vanden AbeeleSaint-Laurent, Quebec, H4S 1R9, Canadaphone 514.335.0792

800.361.1905 (Canada only)fax 514.335.3482e-mail [email protected] www.harvardapparatus.ca

FRANCE Harvard Apparatus, S.A.R.L.Attn: Sales Department6 Avenue des AndesMiniparc – Bat. 891952 Les Ulis Cedex, Francephone 33.1.64.46.00.85Fax 33.1.64.46.94.38e-mail [email protected] www.harvardapparatus.fr

GERMANY Hugo Sachs ElektronikHarvard Apparatus, GmbHGruenstrasse 1D-79232 March-Hugstetten, Germanyphone 49.7665.92000fax 49.7665.920090e-mail [email protected] www.hugo-sachs.de

SPAIN Panlab, S.L.Harvard Apparatus SpainC/Energia, 11208940 Cornellà, BarcelonaSpainphone 34.934.750.697 (International Sales)phone 934.190.709 (Sales in Spain)fax 34.934.750.699e-mail [email protected] www.panlab.com

SWEDEN CMA Microdialysis, ABBox 2SE-171 18 SolnaSwedenphone +46 8 470 10 00fax +46 8 470 10 50e-mail [email protected] www.microdialysis.com

UNITED KINGDOM Harvard Apparatus, Ltd.Attn: Sales DepartmentFircroft Way, EdenbridgeKent TN8 6HE, United Kingdomphone 44.1732.864001fax 44.1732.863356e-mail [email protected] www.harvardapparatus.co.uk

Harvard Apparatus believes in providing the best technical support. Our support specialists can assist you with pre-sales recommendations and in configuringcomplex infusion or anesthesia set-ups, selecting the appropriate surgical equipment, or selecting the best pump for Microdialysis. After purchase we assist inmaking sure your system performs your tasks to your satisfaction.

Web Services for You!• Easy to find what you want: Search box or product buttons

• Full specifications and descriptions

• On-line operator manuals downloadable as PDF’s

• 1000’s of on-line publications downloadable as PDF’s

• A wide range of physiological, anatomical, biological,

chemical information sources for you to downloadable

• Easy to find product selectors

• Pricing

• On-line e-commerce so you can purchase anytime (US only)

World-wide development & manufacturing producing innovative,

highest performance products validated to global quality standards

With manufacturing plants in Germany, Spain, United Kingdom, & United States and built to pass CB SCHEME, CE, CSA, ETL, RoHS & Weee, UL quality standards.

Note: For Research Use Only. Not for use inhumans unless proper investigational deviceregulations have been followed.

Technical Support

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UNITED STATES Harvard Apparatus84 October Hill RoadHolliston, Massachusetts 01746, USAphone 508.893.8999toll free 800.272.2775 (USA Only)fax 508.429.5732e-mail [email protected] www.harvardapparatus.com

Warner Instruments1125 Dixwell AvenueHamden, Connecticut 06514, USAphone 203.776.0664toll free 800.599.4203 (USA Only)fax 203.776.1278e-mail [email protected] www.warneronline.com

Coulbourn InstrumentsAttn: Sales Department5583 Roosevelt StreetWhitehall, Pennsylvania 18052, USAphone 610.395.3771fax 610.391.1333e-mail [email protected] www.coulbourn.com

CANADA Harvard Apparatus CanadaAttn: Sales Department6010 Vanden AbeeleSaint-Laurent, Quebec, H4S 1R9, Canadaphone 514.335.0792

800.361.1905 (Canada only)fax 514.335.3482e-mail [email protected] www.harvardapparatus.ca

FRANCE Harvard Apparatus, S.A.R.L.Attn: Sales Department6 Avenue des AndesMiniparc – Bat. 891952 Les Ulis Cedex, Francephone 33.1.64.46.00.85Fax 33.1.64.46.94.38e-mail [email protected] www.harvardapparatus.fr

GERMANY Hugo Sachs ElektronikHarvard Apparatus, GmbHGruenstrasse 1D-79232 March-Hugstetten, Germanyphone 49.7665.92000fax 49.7665.920090e-mail [email protected] www.hugo-sachs.de

SPAIN Panlab, S.L.Harvard Apparatus SpainC/Energia, 11208940 Cornellà, BarcelonaSpainphone 34.934.750.697 (International Sales)phone 934.190.709 (Sales in Spain)fax 34.934.750.699e-mail [email protected] www.panlab.com

SWEDEN CMA Microdialysis, ABBox 2SE-171 18 SolnaSwedenphone +46 8 470 10 00fax +46 8 470 10 50e-mail [email protected] www.microdialysis.com

UNITED KINGDOM Harvard Apparatus, Ltd.Attn: Sales DepartmentFircroft Way, EdenbridgeKent TN8 6HE, United Kingdomphone 44.1732.864001fax 44.1732.863356e-mail [email protected] www.harvardapparatus.co.uk

Harvard Apparatus believes in providing the best technical support. Our support specialists can assist you with pre-sales recommendations and in configuringcomplex infusion or anesthesia set-ups, selecting the appropriate surgical equipment, or selecting the best pump for Microdialysis. After purchase we assist inmaking sure your system performs your tasks to your satisfaction.

Web Services for You!• Easy to find what you want: Search box or product buttons

• Full specifications and descriptions

• On-line operator manuals downloadable as PDF’s

• 1000’s of on-line publications downloadable as PDF’s

• A wide range of physiological, anatomical, biological,

chemical information sources for you to downloadable

• Easy to find product selectors

• Pricing

• On-line e-commerce so you can purchase anytime (US only)

World-wide development & manufacturing producing innovative,

highest performance products validated to global quality standards

With manufacturing plants in Germany, Spain, United Kingdom, & United States and built to pass CB SCHEME, CE, CSA, ETL, RoHS & Weee, UL quality standards.

Note: For Research Use Only. Not for use inhumans unless proper investigational deviceregulations have been followed.

Technical Support

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84 October Hill RoadHolliston, MA 01746phone 508.893.8999, toll-free 800.272.2775fax 508.429.5732email [email protected] www.harvardapparatus.com

PRSRT STDUS POSTAGE

PAIDHOLLISTON, MAPERMIT NO. 72

Visit us online at:www.harvardapparatus.com

84 October Hill RoadHolliston, MA 01746

phone 508.893.8999, toll-free 800.272.2775fax 508.429.5732

email [email protected]

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