Agenda  

§  Introduc)on  to    (15  mins)  

§  Hands  on  workflows  in  HLA  Twin  (45  mins)  §  Per-­‐sample  indexing  with  Holotype  HLA  X4  data  

§  High  Quality  7-­‐locus  Holotype  HLA  X2  data  §  Novel  alleles  with  IMGT/HLA  v3.10  

§  Export  consensus  

§  Novel  alleles  with  IMGT/HLA  v3.19  

§  Ques)ons  -­‐  just  put  your  hand  up  –  we’ll  be  right  over!  

Omixon  HLA  Twin™  (So4ware)  

§  SoXware  component  of  Holotype  HLA  

§  Automated  genotyping  aXer  MiSeq  run  

§  Preconfigured  Holotype  HLA-­‐specific  se\ngs  

§  Two  algorithms  for  determining  HLA  genotypes  –  Consensus  Genotyping  (Assembly)    –  Sta)s)cal  Genotyping  (Alignment  to  IMGT/HLA)  

§  Traffic  light  system  for  data  interpreta)on  and  workflow  management  

PASSED  WARNING  FAILED  

The  Traffic  Light  System  

Implementa@on  HLA  Twin  

Summary  cards  

View  budon  

Sample  Details  

Browse  Alignment  

Naviga)on  budons  

3  Hands-­‐on  Workflows  in  Twin    

§  Per-­‐sample  indexing  with  Holotype  HLA  X4  data  

§  High  Quality  7-­‐locus  Holotype  HLA  X2  data  

§  Novel  Allele  Discovery  –  Discover  novel  allele  with  IMGT/HLA  3.10  

§  Export  consensus  

–  Resolve  novel  allele  by  reanalyzing  with  IMGT/HLA  3.19