Amazon Biotechnology CenterAdrian Martin Pohlit, Ph.D.

General Manager

Topics:1. Research Group 6. Technology chain of products/services2. Vision 7. In vitro biotechnology applied to3. Objectives Amazonian plant reproduction4. Human Resources5. Facilities (infrastructure)

Amazonian Biotechnology and Bioeconomy

At CBA our goal is to be a nationally and internationally recognized technological institution that supports

sustainable bioeconomy and technological innovation based on Amazonian Biodiversity.

Institutional Vision

• Promote innovation in products and services in cooperation with processing and

manufacturing companies based on materials from the Amazon biodiversity.

• Prospect technology, new raw materials and biomolecules derived from

Amazonian biodiversity with potential for sustainable economic use.

• Provide technological services and quality systems to meet demands of bio-

based industries and public institutions in the Amazon region.

• Contribute to capacity building and the training of human resources in

technologies that support bioeconomy in the Amazon region.

• Densify technology and add value to raw material supply chains based on

Amazon biodiversity.

Strategic objectives

Facilities - Infrastructure

Facilities

Constructed area:12,000 m²

25 laboratories4 industrial units

- Showroom / Exhibition

Hall

- Restaurant

- Administration building

- Guest accommodations

FacilitiesBuilding A: 12 laboratories

floor 1• Microbiology• CQ microbiological• Fermentation• Biochemistry• Genetic sequencing

floor 2• Pharmacotechniques cosmetics• Famacotecnica drugs• Food Development• CQ of food / water

Building B: Analytical Center

• mass spectrometer high resoução Q-Tof

• mass spectrometer Maldi-tof• 5 chromatographs liquid several

detectors• GC-MS and GC-FID• Atomic Absorption

Spectrophotometer• Detection of metals by plasma

ICP-MS

Building B: Analytical Center

• Nuclear magnetic resonance

Building D: Chemistry and Pharmacognosy / Plant

biotechnology• Production of plant extracts• Chemistry of Natural

Products• Separation molecules• Vegetative propagation by

germplasm• seedling production in vitro

Building C: Pharmacology

• In vitro Pharmacology• Toxicology and

Pharmacokinetics• Alternative methods• Biotery

• Central utilities• Pilot plant• Business incubator

New polymeric

biomaterials, textiles,

fabrics, biofuels

RAW MATERIAL

(PLANT,

MICROORGANISM)

INTERMEDIATE

(INGREDIENT,

BIOACTIVE

AGENT)

Phytotherapeutic agents,

pharmaceuticals

TECHNOLOGY CHAIN: RAW MATERIALS, PRODUCTS, SERVICES FROM BIODIVERSITY

Type II Industries

Type III Industries

Dry plants,

packaged

food,

aromas,

medicinals

Extracts: nutritional,

aromatic, medicinal

Biomolecules, markers,

chemical standards

Modified biomolecules,

derivatives

FINISHED

PRODUCT

(ON THE

SHELF)

Foodstuffs, fermented

products, beverages,

energy drinks, etc.

Paints, cosmetics,

perfumes, disinfectants,

insect repellents, etc.

Industrial micro

organisms, enzymes, etc.

TECHNOLOGICAL

DENSIFICATION

MARKETABLE

Biomaterials, fibers, etc.

BIODIVERSITY

COMPONENT

Fresh

plants,

nuts, fruit,

raw

materials

Type I Industries

Type 0 Industries

• Prospecting potential: foods, medicinals, cosmetics, etc.

• Distribution and occurrence of the species

• Establishment of origin, georeferencing

• Collection, botanic characterization, depositing in

collections

• Regulatory documentation for access to genetic

property

• Establishment of socioenvironmental parameters.

• Support for productive systems thru plant biotechnology

tools: selection, improvement, cloning, production of

plantlets, pest control.

IDENTIFICATION

QUALIFICATION

CBA support thru process-based technologies for type 0 industries1

1Type 0 Industry = agroindustries and related producers

Type 0

Industries

BIODIVERSITY

COMPONENT

Fresh plants,

nuts, fruit,

extracted raw

materials, etc.

• Pharmacognostic characterization

• Parameters for drying and storage

• Chemical and microbiological quality control ofplant raw materials (PRMs)

• Quality parameters for production, storageand commercialization

• Development / process improvement

• Viability of industrial production of PRMs

PRIMARY PROCESSING

CBA support thru process-based technologies for type 1 industries1

Type I Industries

RAW MATERIAL

(PLANT,

MICROORGANISM)

Dry plants,

packaged foods,

aromatic and

medicinal plants

• Extraction and separation of molecules

• Chemical characterization

• Potential applications of extracts and molecules

• Physicochemical analyses and definition ofmetrological parameters for quality control.

• Chemical and microbiological quality control

• Process development and improvement

• Process scale-up

• Economicity and productivity parameters

• Proof of efficacy and safety

SECONDARY PROCESSING

CBA support thru process-based technologiesfor type 2 industries

Type II Industries

INTERMEDIATE

(INGREDIENT,

BIOACTIVE AGENT)

Extracts: foods, aromatic

and medicinal

Biomolecules, markers,

chemical standards

Modified biomolecules and

derivatives

Industrial microorganisms,

enzymes, etc.

Biomaterials, fibers, etc.

• Formulation development

• Compatibility and stability studies

• Physicochemical characterization

• Chemical / microbiological quality

control

• Process development and

optimization

• Proof of efficacy and safety

SECONDARY

PROCESSING

CBA support thru process-based technologies

for type 3 industries

Type III

Industries

FINISHED PRODUCT (ON

THE SHELF)

New polymeric

biomaterials, textiles,

fabrics, biofuels, etc.

Phytotherapeutic agentes,

phytopharmaceuticals

Foodstuffs, fermented

products, beverages,

energy drinks, etc.

Pigments, perfumes,

aromas, disinfectants,

insect repellents, etc.

CBA: Cell Culture Laboratory and Micropropagation

CBA: Growing Room

Capacity: 242,000 plantlets

CBA: Green House Infrastructure

Capacity: 10,000 – 30,000 young plants

CBA: Medicinal Plants

Projetos Tempo de

cultivo

T. M. Cultivo

in vitro

T. M. Cultivo

Convencional

Cephaelis

ipecacuanha60 dias 1:4 1:1

Quassia

amara60 dias 1:4 1:1

Simaba

cedron60 dias 1:5 1:1

Ocimum

basilicum30 dias 1:3 1:1

Cephaelis ipecacuanha

Ocimum basilicum

Simaba cedron

“Tulip” Heliconia Dimitri sukri

CBA: Production of Amazonian Ornamental Plants

CBA: Food palmsTucumã (Palm): Astrocaryum aculeatum

Convencional In vitro

Tempo de

germinação

1,5 anos 30 dias

Taxa de

Germinação

50% 70%

30 dias 45 dias

60 dias

CBA: FibersCurauá: Bromeliads

Tempo de

cultivo

T. M. Cultivo

in vitro

T. M. Cultivo

Convencional

Ananas

erectifolius

6 Meses 625 20Ananas

lucidus

Centro de Biotecnologia da Amazônia—CBA Av. Gov. Danilo de Matos Areosa, nº 690 Distrito Industrial Cep: 69075-351-Manaus-AM

Tel: +55 92 3237-2073 www.suframa.gov.br/cba

Thank you!

Adrian Pohlit, Ph.D.

adrian.pohlit@gmail.com