Ang II (+)Ang II (–)

SA-

-gal

D

AP

I

Supplemental Figure 1

Ald

oste

rone

con

cent

ratio

n (n

M)

0

0.2

0.4

0.6

0.8

1

1

Ang II (+)

Ang II (–) **

*

*

**

2 3 4 5 6 7 (days)

*

A

Supplemental Figure 2A

Supplemental Figure 2B,2C

B

*

*

*

0

80

60

40

20

SA

--g

al-p

osit

ive

cell

s(%

of

tota

l cel

ls)

0 0.2 0.4 0.8 1

Aldo (nM)

0

16

4

8

12

**

**p21

0

2

4*

*

p53

0

2

4

6 ***

*

p16

0 0.2 0.4 0.8 1

Aldo (nM)

0

2

4

6

*

**

*p27

Fol

d ch

ange

Fol

d ch

ange

Fol

d ch

ange

Fol

d ch

ange

p21

p53

p16

p27

-SMactin

0 0.2 0.4 0.8 1

Aldo (nM)

C

-SM actin

Ki-ras2A

Ang II+Aldo 0 1 3 (days)

0

*

*

**

3

2

1

4

5

5 7

Ki-

ras2

A(f

old

chan

ge)

Supplemental Figure 3

Name Sequence

S

AS

S

AS

S

AS

Ki-ras2A-15’- CGAAACAGGCUCAGGAGUUTT -3’

5’- AACUCCUGAGCCUGUUUCGTT -3’

5’- CCGAGAAAUUCGAAAACAUTT -3’

5’- AUGUUUUCGAAUUUCUCGGTT -3’

5’- AAGAAGAAGUCAAGGACAATT -3’

5’- UUGUCCUUGACUUCUUCUUTT -3’

Supplemental Table 1: Sequences for cocktail of siRNA targeting Ki-ras2A

Ki-ras2A-2

Ki-ras2A-3

Supplemental Table 1

Name Sequence

S

AS

S

AS

S

AS

5’- GCGCCGUACGAGGUAUAAATT -3’

5’- UUUAUACCUCGUACGGCGCTT -3’

5’- AUAGAACGACAACGCUAUATT -3’

5’- UAUAGCGUUGUCGUUCUAUTT -3’

5’- CGAUUAAGAAACGAAUAGGTT -3’

5’- CCUAUUCGUUUCUUAAUCGTT -3’

Scrambled control-1

Scrambled control-2

Scrambled control-3

Supplemental Table 2: Sequences for cocktail of scrambled version of Ki-ras2A-siRNA as control

Supplemental Table 2