AP Biology Discussion Notes

Wednesday 01/21/2015

Goals for Today• Be able to describe how restriction

enzymes work and why they are valuable to scientists

• Be able to describe & demonstrate gel electrophoresis.

• Be able to stop smugglers and other bad guys using restriction enzymes!

Question of the Day 1/21• In real life, since we couldn’t actually count

base pairs to determine the fragment length, how could we separate fragments of DNA based on length? (What tool or test could we use?)

Biotechnology Virtual Labs

• TURN IN

• Would you expect the DNA from the Downy woodpecker and the Hairy woodpecker to be similar? Would you expect it to be the SAME? Explain your answers.

Restriction EnzymesStop smugglers!

–Form a group of 4 each do a different enzyme

–Fill in your charts about fragment length

–Get blue sheet and analyze your results with gel electrophoresis.

–Finish analysis Questions to help future scientists stop smugglers!

Gel Electrophoresis

Each sample (unknown, elephant, mammoth) will go its own lane of the “Gel” Draw a picture of your gel

Gel Electrophoresis

Each sample (unknown, elephant, mammoth) will go its own lane of the “Gel” Draw a picture of your gel

Restriction EnzymesStop smugglers!

–Form a group of 4 each do a different enzyme

–Fill in your charts about fragment length

–Get blue sheet and analyze your results with gel electrophoresis.

–Finish analysis Questions to help future scientists stop smugglers!

Species of the Day 1/21

• Bald Eagle– Haliaeetus leucocephalus

Species of the Day 1/21

• Bald Eagle– Haliaeetus leucocephalus

Species of the Day 1/21• Bald Eagle

– Haliaeetus leucocephalus

American Robin

Species of the Day 1/21• Bald Eagle

– Haliaeetus leucocephalus

If I have a DNA sequence from a Leucistic eagle that appears to only differ at one base pair sequence from other traditionally colored eagles, how could I test to see if this was true for other traditionally colored eagles & Leucistic eagles without sequencing their DNA? Explain how you would do this!